RchiOBHm_Chr2g0153031

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003698
Physical Location & Seq
Reverse (-)
70421880 .. 70423001
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52215

Sequence Viewer

Length: 768 bp
ATGGGATTGGAATACACTTCACCAGCTTTTGCATCTGCCATGTCCAACATGATTCCATCGATTACCTTCATCTTAGCTGTTTTGTGCAGGATGGAACAGTTTGATATTTCCAAGCCTGGTACCCAAGCCAAGATTGGAGGAACTCTTGCTGCCTTTGCTGGTGCAACACTCATGACCCTCTACAAGGGCGTTGCAGTAATTACAATGCACACCAAAAGCTCCTATCAAACTGCAGGTACCTCAAAATCATCTACACACGCAGAATGGATCAAGGGCTCCCTTGTCCTTATTGTTTCATATAGTTCAGTTGCAGCATCCTATATCTTACAGACATCAACAATTAAAATGTATCCAGCACCAATCACTCTTACATCATTGACCTGCTTATCTGGGACACTGCTCTCAACAATCATGGCAGCAATTCTAGATCACAAAGCATCTTCTTGGAAGTTATCATGGAACACACTTCTTGCTCCTATGTATAGTGGAACTGTGATTTTCGGAGTCACATTTTATGTTCAGACCTTAGTAGTAAAAACAAAAGGCCCAGTTTTCATGGCAGCTTTTAGACCACTAGCTACCATTGTTGTAGCTATAATGGGACTACTCATTTTAGGAGAAGCTATATACTCGGGAAGTGTTTTGGGGGCTTTCTTGATAGTTCTTGGTCTGTATGCAACTCTTTGGGGAAAGAAAAAAGAGAAGGAGAAGAAGTCAATGGATCATGGTATATCTGAGCAAGCTATTGACATCAATTTAGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

255

Amino Acids

27.29

Weight (kDa)

9.4

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 91 - 228 2.2e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 224, 389
Acc65I GGTACC 2 cut(s) 119, 236
AccB1I GGYRCC 2 cut(s) 119, 236
AclWI GGATC 2 cut(s) 275, 729
AfaI GTAC 2 cut(s) 121, 238
AfiI CCNNNNNNNGG 1 cut(s) 184
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 8 cut(s) 26, 77, 219, 563, 578, 593, 623, 743
AluI AGCT 8 cut(s) 26, 77, 219, 563, 578, 593, 623, 743
AlwI GGATC 2 cut(s) 275, 729
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 4 cut(s) 149, 311, 416, 560
Asp718I GGTACC 2 cut(s) 119, 236
AspS9I GGNCC 1 cut(s) 545
AsuHPI GGTGA 1 cut(s) 12
AvaI CYCGRG 1 cut(s) 631
BanI GGYRCC 2 cut(s) 119, 236
BanII GRGCYC 1 cut(s) 278
BbvI GCAGC 4 cut(s) 136, 323, 428, 572
BccI CCATC 2 cut(s) 64, 85
BciT130I CCWGG 1 cut(s) 117
BciVI GTATCC 1 cut(s) 360
BfaI CTAG 2 cut(s) 425, 575
BfmI CTRYAG 1 cut(s) 231
BfuAI ACCTGC 2 cut(s) 224, 389
BfuI GTATCC 1 cut(s) 360
BisI GCNGC 4 cut(s) 150, 312, 417, 561
BlsI GCNGC 4 cut(s) 151, 313, 418, 562
Bme1390I CCNGG 1 cut(s) 117
BmeT110I CYCGRG 1 cut(s) 631
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 3 cut(s) 121, 238, 277
BmrFI CCNGG 1 cut(s) 117
BmrI ACTGGG 1 cut(s) 542
BmsI GCATC 3 cut(s) 41, 323, 446
BmuI ACTGGG 1 cut(s) 542
Bsa29I ATCGAT 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 698, 728
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse1I ACTGG 1 cut(s) 548
BseBI CCWGG 1 cut(s) 117
BseCI ATCGAT 1 cut(s) 59
BseGI GGATG 2 cut(s) 96, 314
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 1 cut(s) 548
BseXI GCAGC 4 cut(s) 136, 323, 428, 572
BsgI GTGCAG 1 cut(s) 106
BshFI GGCC 1 cut(s) 546
BshNI GGYRCC 2 cut(s) 119, 236
BshVI ATCGAT 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 631
BslFI GGGAC 2 cut(s) 406, 615
BslI CCNNNNNNNGG 1 cut(s) 184
BsmFI GGGAC 2 cut(s) 406, 615
BsnI GGCC 1 cut(s) 546
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 278
Bsp143I GATC 3 cut(s) 267, 427, 721
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 1 cut(s) 727
BspDI ATCGAT 1 cut(s) 59
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 3 cut(s) 121, 238, 277
BspMAI CTGCAG 1 cut(s) 235
BspMI ACCTGC 2 cut(s) 224, 389
BspPI GGATC 2 cut(s) 275, 729
BspT107I GGYRCC 2 cut(s) 119, 236
BsrI ACTGG 1 cut(s) 548
BssMI GATC 3 cut(s) 267, 427, 721
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 99, 493
BstC8I GCNNGC 1 cut(s) 741
BstDEI CTNAG 3 cut(s) 73, 526, 735
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 2 cut(s) 96, 314
BstKTI GATC 3 cut(s) 270, 430, 724
BstMBI GATC 3 cut(s) 267, 427, 721
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 4 cut(s) 136, 323, 428, 572
Bsu15I ATCGAT 1 cut(s) 59
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 1 cut(s) 546
BsuTUI ATCGAT 1 cut(s) 59
BtsCI GGATG 2 cut(s) 96, 314
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 1 cut(s) 395
BveI ACCTGC 2 cut(s) 224, 389
Cac8I GCNNGC 1 cut(s) 741
CciI TCATGA 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 545
ClaI ATCGAT 1 cut(s) 59
Csp6I GTAC 2 cut(s) 120, 237
CviAII CATG 7 cut(s) 40, 49, 172, 412, 456, 556, 725
CviQI GTAC 2 cut(s) 120, 237
DdeI CTNAG 3 cut(s) 73, 526, 735
DpnI GATC 3 cut(s) 269, 429, 723
DpnII GATC 3 cut(s) 267, 427, 721
Eco24I GRGCYC 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 631
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 278
FaeI CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
FaqI GGGAC 2 cut(s) 406, 615
FatI CATG 7 cut(s) 39, 48, 171, 411, 455, 555, 724
Fnu4HI GCNGC 4 cut(s) 150, 312, 417, 561
FokI GGATG 2 cut(s) 103, 301
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 4 cut(s) 150, 312, 417, 561
FspBI CTAG 2 cut(s) 425, 575
GluI GCNGC 4 cut(s) 150, 312, 417, 561
HaeIII GGCC 1 cut(s) 546
Hin1II CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
HinfI GANTC 2 cut(s) 52, 504
HphI GGTGA 1 cut(s) 12
Hpy188I TCNGA 3 cut(s) 503, 522, 736
Hpy188III TCNNGA 4 cut(s) 172, 425, 633, 655
HpyAV CCTTC 2 cut(s) 76, 697
HpyCH4III ACNGT 2 cut(s) 99, 493
HpyCH4V TGCA 8 cut(s) 32, 87, 164, 194, 208, 233, 311, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 3 cut(s) 73, 526, 735
Hsp92II CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
KpnI GGTACC 2 cut(s) 123, 240
Kzo9I GATC 3 cut(s) 267, 427, 721
LmnI GCTCC 3 cut(s) 224, 281, 478
Lsp1109I GCAGC 4 cut(s) 136, 323, 428, 572
LweI GCATC 3 cut(s) 41, 323, 446
MaeI CTAG 2 cut(s) 425, 575
MaeIII GTNAC 1 cut(s) 505
MalI GATC 3 cut(s) 269, 429, 723
MboI GATC 3 cut(s) 267, 427, 721
MboII GAAGA 2 cut(s) 432, 721
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 4 cut(s) 198, 339, 420, 754
MlyI GAGTC 1 cut(s) 513
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 3 cut(s) 131, 188, 250
MseI TTAA 1 cut(s) 342
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 155
NdeII GATC 3 cut(s) 267, 427, 721
NlaIII CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
NlaIV GGNNCC 3 cut(s) 121, 238, 277
NmuCI GTSAC 1 cut(s) 505
PagI TCATGA 1 cut(s) 171
PfeI GAWTC 1 cut(s) 52
PkrI GCNGC 4 cut(s) 151, 313, 418, 562
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 121, 238, 277
PspPI GGNCC 1 cut(s) 545
PstI CTGCAG 1 cut(s) 235
RsaI GTAC 2 cut(s) 121, 238
RsaNI GTAC 2 cut(s) 120, 237
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 4 cut(s) 150, 312, 417, 561
Sau3AI GATC 3 cut(s) 267, 427, 721
Sau96I GGNCC 1 cut(s) 545
SchI GAGTC 1 cut(s) 513
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 278
SfaNI GCATC 3 cut(s) 41, 323, 446
SfcI CTRYAG 1 cut(s) 231
Sse9I AATT 4 cut(s) 198, 339, 420, 754
SspMI CTAG 2 cut(s) 425, 575
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 99, 493
TaqI TCGA 1 cut(s) 59
TasI AATT 4 cut(s) 198, 339, 420, 754
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 4 cut(s) 149, 311, 416, 560
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 58, 285, 544
TspRI CASTG 1 cut(s) 402
XagI CCTNNNNNAGG 1 cut(s) 182
XbaI TCTAGA 1 cut(s) 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 2 cut(s) 425, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.