RchiOBHm_Chr2g0156811

Thymidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006534
Physical Location & Seq
Reverse (-)
73359270 .. 73361056
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52558

Sequence Viewer

Length: 777 bp
ATGAAACTCTTCCTCTTCCCATCTTCTCTCTCCTTCTCTTCCCTAATTTTCAATCCCTTTTCTCACTGTAACCCTAAATCCATGGCCTCCTTCAAACCCACAATCCCCCTGACCAGCGAATCCGCCCCTTCCCGCCGCCCCCCCTCGGGCGAGGTCCACGTCATCATGGGTCCCATGTTCGCCGGCAAGACCACCGCTCTCCTGCGCCGGATCAAGTCCGAGGGCGCCAACGGCAGGAATGTGGCGATGATAAAATCGAGCAAGGATAACAGATACGCCGTCGATTCGGTGGTGACTCATGATGGGGCGAAATTTCCATGCTGGGCTCTGCCGGATCTGTCCTCGTTTAGGCACAAATTTGGAGGTGATGCTTATGATAAGCTTGATGTTATTGGTATCGATGAGGCTCAATTTTTCGAAGATCTATATGATTTCTGCTGCACGGCTGCTGATCATGATGGGAAAACTGTGATTGTTGCAGGCTTGGATGGCGATTACTTAAGGAAAAGCTTTGGCTCGGTGCTTGATGTAGTGCCTCTTGCTGATACAGTGACCAAATTGACAGCTCGATGTGAAATGTGTGGCAAACGAGCTTTCTTTACCCTGAGGAAGACGGAGGAGACGAAGACTGAACTGATTGGCGGGGCTGATGTCTACATGCCTGTTTGTCGCCAACACTATGTCAATGGACAAGTTCTCATCAAATCTGCAAGAAGTGTTGTGGAGTCTCATAAATTTAAGAGTGGCACCATTTCAGAAGCAGCTGCAGTTATTTAG

Protein Analysis

258

Amino Acids

28.23

Weight (kDa)

8.62

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TK PF00265 50 - 227 1.3e-53 Thymidine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 224, 746
AccBSI CCGCTC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 654
AciI CCGC 5 cut(s) 123, 133, 136, 195, 642
AclWI GGATC 2 cut(s) 218, 342
AcsI RAATTY 3 cut(s) 311, 356, 734
AcyI GRCGYC 1 cut(s) 225
AfiI CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
AflII CTTAAG 1 cut(s) 499
AgsI TTSAA 2 cut(s) 52, 94
AjiI CACGTC 1 cut(s) 160
AluBI AGCT 5 cut(s) 382, 510, 566, 593, 764
AluI AGCT 5 cut(s) 382, 510, 566, 593, 764
Alw26I GTCTC 2 cut(s) 614, 732
AlwI GGATC 2 cut(s) 218, 342
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 4 cut(s) 438, 446, 761, 764
ApoI RAATTY 3 cut(s) 311, 356, 734
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 2 cut(s) 207, 227
AspS9I GGNCC 2 cut(s) 154, 170
AsuHPI GGTGA 2 cut(s) 304, 377
AsuII TTCGAA 1 cut(s) 417
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 2 cut(s) 154, 170
AxyI CCTNAGG 1 cut(s) 605
BanI GGYRCC 2 cut(s) 224, 746
BanII GRGCYC 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 617, 632
BbvI GCAGC 4 cut(s) 425, 433, 751, 773
BccI CCATC 4 cut(s) 28, 296, 452, 482
BceAI ACGGC 3 cut(s) 247, 263, 459
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 2 cut(s) 614, 732
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 1 cut(s) 228
BfrI CTTAAG 1 cut(s) 499
BglII AGATCT 1 cut(s) 421
BisI GCNGC 5 cut(s) 136, 439, 447, 762, 765
BlsI GCNGC 5 cut(s) 137, 440, 448, 763, 766
Bme18I GGWCC 2 cut(s) 154, 170
BmeT110I CYCGRG 1 cut(s) 145
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 154, 170
BmiI GGNNCC 4 cut(s) 171, 172, 226, 748
BmsI GCATC 1 cut(s) 358
BpiI GAAGAC 2 cut(s) 617, 632
Bpu14I TTCGAA 1 cut(s) 417
Bsa29I ATCGAT 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 81, 144, 219
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
Bse118I RCCGGY 1 cut(s) 182
Bse21I CCTNAGG 1 cut(s) 605
BseCI ATCGAT 1 cut(s) 399
BseDI CCNNGG 3 cut(s) 81, 144, 219
BseGI GGATG 1 cut(s) 493
BseLI CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
BseMII CTCAG 1 cut(s) 596
BseRI GAGGAG 1 cut(s) 632
BseXI GCAGC 4 cut(s) 425, 433, 751, 773
BseYI CCCAGC 1 cut(s) 321
BsgI GTGCAG 1 cut(s) 424
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 2 cut(s) 224, 746
BshVI ATCGAT 1 cut(s) 399
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 3 cut(s) 183, 208, 332
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
BsmAI GTCTC 2 cut(s) 614, 732
BsmBI CGTCTC 1 cut(s) 614
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 145
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 4 cut(s) 210, 334, 421, 451
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 5 cut(s) 123, 133, 136, 195, 642
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 597
BspDI ATCGAT 1 cut(s) 399
BspHI TCATGA 2 cut(s) 298, 454
BspLI GGNNCC 4 cut(s) 171, 172, 226, 748
BspMAI CTGCAG 1 cut(s) 769
BspPI GGATC 2 cut(s) 218, 342
BspT104I TTCGAA 1 cut(s) 417
BspT107I GGYRCC 2 cut(s) 224, 746
BspTI CTTAAG 1 cut(s) 499
BsrBI CCGCTC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 3 cut(s) 81, 144, 219
BssMI GATC 4 cut(s) 210, 334, 421, 451
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 3 cut(s) 68, 469, 550
Bst6I CTCTTC 3 cut(s) 14, 20, 43
BstACI GRCGYC 1 cut(s) 225
BstAFI CTTAAG 1 cut(s) 499
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 184, 481
BstDEI CTNAG 1 cut(s) 605
BstDSI CCRYGG 1 cut(s) 81
BstENI CCTNNNNNAGG 1 cut(s) 346
BstF5I GGATG 1 cut(s) 493
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 2 cut(s) 207, 227
BstKTI GATC 4 cut(s) 213, 337, 424, 454
BstMAI GTCTC 2 cut(s) 614, 732
BstMBI GATC 4 cut(s) 210, 334, 421, 451
BstMWI GCNNNNNNNGC 2 cut(s) 231, 489
BstNSI RCATGY 1 cut(s) 661
BstSFI CTRYAG 1 cut(s) 765
BstV1I GCAGC 4 cut(s) 425, 433, 751, 773
BstV2I GAAGAC 2 cut(s) 617, 632
BstX2I RGATCY 2 cut(s) 334, 421
BstYI RGATCY 2 cut(s) 334, 421
Bsu15I ATCGAT 1 cut(s) 399
Bsu36I CCTNAGG 1 cut(s) 605
BsuRI GGCC 1 cut(s) 86
BsuTUI ATCGAT 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 260
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 1 cut(s) 493
BtsIMutI CAGTG 2 cut(s) 64, 555
Cac8I GCNNGC 2 cut(s) 184, 481
CciI TCATGA 2 cut(s) 298, 454
CfoI GCGC 2 cut(s) 207, 227
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 154, 170
ClaI ATCGAT 1 cut(s) 399
CviAII CATG 7 cut(s) 82, 166, 175, 299, 318, 455, 658
DdeI CTNAG 1 cut(s) 605
DinI GGCGCC 1 cut(s) 226
DpnI GATC 4 cut(s) 212, 336, 423, 453
DpnII GATC 4 cut(s) 210, 334, 421, 451
Eam1104I CTCTTC 3 cut(s) 14, 20, 43
EarI CTCTTC 3 cut(s) 14, 20, 43
EciI GGCGGA 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 328
Eco47I GGWCC 2 cut(s) 154, 170
Eco81I CCTNAGG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 328
EgeI GGCGCC 1 cut(s) 226
EheI GGCGCC 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 614
FaeI CATG 7 cut(s) 85, 169, 178, 302, 321, 458, 661
FaqI GGGAC 1 cut(s) 156
FatI CATG 7 cut(s) 81, 165, 174, 298, 317, 454, 657
FauI CCCGC 2 cut(s) 140, 635
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 5 cut(s) 136, 439, 447, 762, 765
FokI GGATG 1 cut(s) 500
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 5 cut(s) 136, 439, 447, 762, 765
GlaI GCGC 2 cut(s) 206, 226
GluI GCNGC 5 cut(s) 136, 439, 447, 762, 765
GsaI CCCAGC 1 cut(s) 325
HaeII RGCGCY 1 cut(s) 228
HaeIII GGCC 1 cut(s) 86
HapII CCGG 3 cut(s) 183, 208, 332
HhaI GCGC 2 cut(s) 207, 227
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 7 cut(s) 85, 169, 178, 302, 321, 458, 661
Hin6I GCGC 2 cut(s) 205, 225
HinP1I GCGC 2 cut(s) 205, 225
HindIII AAGCTT 2 cut(s) 380, 508
HinfI GANTC 4 cut(s) 119, 284, 295, 725
HpaII CCGG 3 cut(s) 183, 208, 332
HphI GGTGA 2 cut(s) 304, 377
Hpy166II GTNNAC 2 cut(s) 157, 655
Hpy188I TCNGA 2 cut(s) 220, 757
Hpy188III TCNNGA 2 cut(s) 299, 455
Hpy8I GTNNAC 2 cut(s) 157, 655
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 43, 100, 138
HpyCH4III ACNGT 3 cut(s) 68, 469, 550
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 4 cut(s) 441, 479, 710, 767
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 489
HpyF3I CTNAG 1 cut(s) 605
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 7 cut(s) 85, 169, 178, 302, 321, 458, 661
HspAI GCGC 2 cut(s) 205, 225
KasI GGCGCC 1 cut(s) 224
KflI GGGWCCC 1 cut(s) 170
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 4 cut(s) 210, 334, 421, 451
Lsp1109I GCAGC 4 cut(s) 425, 433, 751, 773
LweI GCATC 1 cut(s) 358
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 3 cut(s) 68, 292, 550
MalI GATC 4 cut(s) 212, 336, 423, 453
MbiI CCGCTC 1 cut(s) 197
MboI GATC 4 cut(s) 210, 334, 421, 451
MboII GAAGA 6 cut(s) 7, 15, 30, 431, 622, 637
MflI RGATCY 2 cut(s) 334, 421
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 6 cut(s) 45, 311, 356, 410, 557, 734
Mly113I GGCGCC 1 cut(s) 225
MlyI GAGTC 2 cut(s) 289, 734
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 500, 738
MspA1I CMGCKG 1 cut(s) 764
MspCI CTTAAG 1 cut(s) 499
MspI CCGG 3 cut(s) 183, 208, 332
MwoI GCNNNNNNNGC 2 cut(s) 231, 489
NaeI GCCGGC 1 cut(s) 184
NarI GGCGCC 1 cut(s) 225
NcoI CCATGG 1 cut(s) 81
NdeII GATC 4 cut(s) 210, 334, 421, 451
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 7 cut(s) 85, 169, 178, 302, 321, 458, 661
NlaIV GGNNCC 4 cut(s) 171, 172, 226, 748
NmuCI GTSAC 2 cut(s) 292, 550
NspI RCATGY 1 cut(s) 661
NspV TTCGAA 1 cut(s) 417
PagI TCATGA 2 cut(s) 298, 454
PcsI WCGNNNNNNNCGW 1 cut(s) 620
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 119, 284
PkrI GCNGC 5 cut(s) 137, 440, 448, 763, 766
PleI GAGTC 2 cut(s) 289, 733
PluTI GGCGCC 1 cut(s) 228
PpsI GAGTC 2 cut(s) 289, 733
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 321
PspN4I GGNNCC 4 cut(s) 171, 172, 226, 748
PspPI GGNCC 2 cut(s) 154, 170
PspPPI RGGWCCY 1 cut(s) 170
PstI CTGCAG 1 cut(s) 769
PsuI RGATCY 2 cut(s) 334, 421
PvuII CAGCTG 1 cut(s) 764
SaqAI TTAA 2 cut(s) 500, 738
SatI GCNGC 5 cut(s) 136, 439, 447, 762, 765
Sau3AI GATC 4 cut(s) 210, 334, 421, 451
Sau96I GGNCC 2 cut(s) 154, 170
SchI GAGTC 2 cut(s) 289, 734
SduI GDGCHC 1 cut(s) 328
SetI ASST 8 cut(s) 156, 162, 367, 384, 512, 568, 595, 766
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 765
SfoI GGCGCC 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 2 cut(s) 154, 170
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 6 cut(s) 45, 311, 356, 410, 557, 734
SsiI CCGC 5 cut(s) 123, 133, 136, 195, 642
SspDI GGCGCC 1 cut(s) 224
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 68, 469, 550
TaiI ACGT 1 cut(s) 162
TaqI TCGA 5 cut(s) 257, 282, 399, 417, 568
TasI AATT 6 cut(s) 45, 311, 356, 410, 557, 734
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 2 cut(s) 119, 284
Tru1I TTAA 2 cut(s) 500, 738
Tru9I TTAA 2 cut(s) 500, 738
TscAI CASTG 2 cut(s) 71, 555
TseFI GTSAC 2 cut(s) 292, 550
TseI GCWGC 4 cut(s) 438, 446, 761, 764
Tsp45I GTSAC 2 cut(s) 292, 550
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 2 cut(s) 71, 555
Vha464I CTTAAG 1 cut(s) 499
VpaK11BI GGWCC 2 cut(s) 154, 170
XagI CCTNNNNNAGG 1 cut(s) 346
XapI RAATTY 3 cut(s) 311, 356, 734
XceI RCATGY 1 cut(s) 661
XmiI GTMKAC 1 cut(s) 654
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.