RchiOBHm_Chr4g0402221

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002370
Physical Location & Seq
Forward (+)
20733632 .. 20734558
927 bp
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UTR
Exon/CDS
Intron
PRQ37404

Sequence Viewer

Length: 861 bp
ATGTTTTACCTTAGCTATCTTATTTGTATGGGGGTGAAATTTGCACACCCCCTTTTACATTCCACACACCCTTTGCATTTTTTTAATATTTCTTATCAATCCACTATATTTCTCTTCCAAAACTACCCTTCTCTCTCTCTCCCCCCCCTCCCTCCCTCCCGGTTTCCATCTGAATCTGAATCTGAGTCTGACCCATCTCACTGTAATCCTCCGCTACCCACATCCCAACATCAAGAACAATCTTTATCTCCAAACCAAGTCCCGGAAGTTGCAGAACCAGCGATCCTGGGATCATCGTCGCCGCCGCCGTCGGAGCTAGACTTCCCAATCGAATGGCCCCCCCACGGGAATCTCAGCCTGGACTGGGTTCTTCCCGTCCGAGTTTTCGATTCCTTGGCTCTTTGCGCCTCCAAGATTCTCCACAAAGAACCCAATTGTGTCAAGATCGACGACTGCGGCCCTAACTCCACCGCCGTCGTCGGAGACGTCCACAGCCAGTTGCACGACGTTCTTTTTCTTCTGAAGGATGCTGGGTACCCTTCGGAGAATCGAGTCTTTGTCTTCAATGGGGATTATGTTGACAGAGGAGCTTGGGGTCTTGAGACTTTCTTGATTTTACTGGCTTGGAAAGTGTTGATGCTGAAAAGCGTTTATCTTTTGAGAGGTAACCATGAATCCAAGTACTGTACTTCTGTTTATGGGTTTGAGAAGGAAGTGCTAGTCAAGTATAGAGATGACGATGGTAAAGATAAAGTAGGTAAACATGTGTATAGAAAATGCCTGGGATGCTTTGAAAGGCTTCCTCTTACGTCCATTATAAATGGCCATGTATATACTGCTCATGGAGGGCTAGCTGTTTAG

Protein Analysis

286

Amino Acids

32.02

Weight (kDa)

5.93

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 158 - 283 2.5e-21 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 818
AatII GACGTC 1 cut(s) 489
Acc65I GGTACC 1 cut(s) 534
AccB1I GGYRCC 1 cut(s) 534
AciI CCGC 5 cut(s) 212, 302, 305, 456, 471
AclWI GGATC 2 cut(s) 277, 298
AcoI YGGCCR 1 cut(s) 823
AcsI RAATTY 1 cut(s) 38
AcuI CTGAAG 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 3 cut(s) 536, 683, 688
AfiI CCNNNNNNNGG 4 cut(s) 262, 344, 345, 364
AflIII ACRYGT 1 cut(s) 763
AgsI TTSAA 2 cut(s) 565, 794
AjnI CCWGG 3 cut(s) 285, 357, 780
AloI GAACNNNNNNTCC 2 cut(s) 267, 299
AluBI AGCT 4 cut(s) 15, 316, 590, 854
AluI AGCT 4 cut(s) 15, 316, 590, 854
Alw26I GTCTC 2 cut(s) 477, 596
AlwI GGATC 2 cut(s) 277, 298
AoxI GGCC 3 cut(s) 335, 457, 823
ApoI RAATTY 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 798
Asp718I GGTACC 1 cut(s) 534
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 2 cut(s) 336, 458
AsuC2I CCSGG 2 cut(s) 160, 263
AsuHPI GGTGA 1 cut(s) 46
AsuNHI GCTAGC 1 cut(s) 850
BalI TGGCCA 1 cut(s) 825
BanI GGYRCC 1 cut(s) 534
BbsI GAAGAC 1 cut(s) 553
BccI CCATC 3 cut(s) 175, 202, 734
BceAI ACGGC 2 cut(s) 292, 458
BciT130I CCWGG 3 cut(s) 287, 359, 782
BcnI CCSGG 2 cut(s) 160, 263
BcoDI GTCTC 2 cut(s) 477, 596
BfaI CTAG 3 cut(s) 317, 719, 851
BisI GCNGC 3 cut(s) 302, 305, 457
BlsI GCNGC 3 cut(s) 303, 306, 458
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 5 cut(s) 160, 263, 287, 359, 782
BmgT120I GGNCC 2 cut(s) 336, 458
BmiI GGNNCC 2 cut(s) 338, 536
BmrFI CCNGG 5 cut(s) 160, 263, 287, 359, 782
BmrI ACTGGG 1 cut(s) 373
BmsI GCATC 3 cut(s) 517, 627, 776
BmtI GCTAGC 1 cut(s) 854
BmuI ACTGGG 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 553
Bpu10I CCTNAGC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 2 cut(s) 160, 263
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 4 cut(s) 286, 343, 393, 781
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 344, 345, 364
Bse1I ACTGG 3 cut(s) 368, 496, 624
BseBI CCWGG 3 cut(s) 287, 359, 782
BseDI CCNNGG 4 cut(s) 286, 343, 393, 781
BseGI GGATG 3 cut(s) 221, 532, 791
BseLI CCNNNNNNNGG 4 cut(s) 262, 344, 345, 364
BseMII CTCAG 2 cut(s) 174, 367
BseNI ACTGG 3 cut(s) 368, 496, 624
BseRI GAGGAG 1 cut(s) 600
BseYI CCCAGC 1 cut(s) 530
BshFI GGCC 3 cut(s) 337, 459, 825
BshNI GGYRCC 1 cut(s) 534
BsiSI CCGG 2 cut(s) 160, 263
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 4 cut(s) 262, 344, 345, 364
BsmAI GTCTC 2 cut(s) 477, 596
BsmBI CGTCTC 1 cut(s) 477
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 3 cut(s) 337, 459, 825
Bsp143I GATC 3 cut(s) 282, 290, 444
BspACI CCGC 5 cut(s) 212, 302, 305, 456, 471
BspANI GGCC 3 cut(s) 337, 459, 825
BspCNI CTCAG 2 cut(s) 175, 366
BspLI GGNNCC 2 cut(s) 338, 536
BspOI GCTAGC 1 cut(s) 854
BspPI GGATC 2 cut(s) 277, 298
BspT107I GGYRCC 1 cut(s) 534
BsrI ACTGG 3 cut(s) 368, 496, 624
BssECI CCNNGG 4 cut(s) 286, 343, 393, 781
BssMI GATC 3 cut(s) 282, 290, 444
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 3 cut(s) 287, 359, 782
Bst4CI ACNGT 2 cut(s) 203, 686
Bst6I CTCTTC 1 cut(s) 119
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 852
BstDEI CTNAG 3 cut(s) 11, 183, 353
BstDSI CCRYGG 1 cut(s) 343
BstEII GGTNACC 1 cut(s) 665
BstF5I GGATG 3 cut(s) 221, 532, 791
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 3 cut(s) 285, 293, 447
BstMAI GTCTC 2 cut(s) 477, 596
BstMBI GATC 3 cut(s) 282, 290, 444
BstMWI GCNNNNNNNGC 4 cut(s) 278, 313, 404, 786
BstNI CCWGG 3 cut(s) 287, 359, 782
BstNSI RCATGY 1 cut(s) 767
BstPI GGTNACC 1 cut(s) 665
BstSCI CCNGG 5 cut(s) 158, 261, 285, 357, 780
BstV2I GAAGAC 1 cut(s) 553
BstXI CCANNNNNNTGG 1 cut(s) 333
BsuRI GGCC 3 cut(s) 337, 459, 825
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 3 cut(s) 221, 532, 791
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 852
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 2 cut(s) 336, 458
Csp6I GTAC 3 cut(s) 535, 682, 687
CviAII CATG 4 cut(s) 671, 764, 827, 842
CviQI GTAC 3 cut(s) 535, 682, 687
DdeI CTNAG 3 cut(s) 11, 183, 353
DpnI GATC 3 cut(s) 284, 292, 446
DpnII GATC 3 cut(s) 282, 290, 444
EaeI YGGCCR 1 cut(s) 823
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 393
Eco57I CTGAAG 1 cut(s) 542
Eco91I GGTNACC 1 cut(s) 665
EcoO65I GGTNACC 1 cut(s) 665
EcoRII CCWGG 3 cut(s) 285, 357, 780
EcoT14I CCWWGG 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 1 cut(s) 477
FaeI CATG 4 cut(s) 674, 767, 830, 845
FaqI GGGAC 1 cut(s) 245
FatI CATG 4 cut(s) 670, 763, 826, 841
Fnu4HI GCNGC 3 cut(s) 302, 305, 457
FokI GGATG 3 cut(s) 208, 539, 798
Fsp4HI GCNGC 3 cut(s) 302, 305, 457
FspBI CTAG 3 cut(s) 317, 719, 851
GlaI GCGC 1 cut(s) 406
GluI GCNGC 3 cut(s) 302, 305, 457
GsaI CCCAGC 1 cut(s) 534
HaeIII GGCC 3 cut(s) 337, 459, 825
HapII CCGG 2 cut(s) 160, 263
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 4 cut(s) 674, 767, 830, 845
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 9 cut(s) 173, 179, 185, 349, 389, 415, 547, 552, 674
HpaII CCGG 2 cut(s) 160, 263
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 3 cut(s) 490, 580, 761
Hpy188I TCNGA 9 cut(s) 172, 178, 184, 190, 313, 380, 482, 522, 544
Hpy188III TCNNGA 4 cut(s) 233, 442, 599, 610
Hpy8I GTNNAC 3 cut(s) 490, 580, 761
Hpy99I CGWCG 6 cut(s) 301, 313, 452, 479, 482, 509
HpyAV CCTTC 4 cut(s) 138, 517, 549, 703
HpyCH4III ACNGT 2 cut(s) 203, 686
HpyCH4IV ACGT 3 cut(s) 486, 507, 809
HpyCH4V TGCA 4 cut(s) 44, 76, 272, 502
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 313, 404, 786
HpyF3I CTNAG 3 cut(s) 11, 183, 353
HpySE526I ACGT 3 cut(s) 486, 507, 809
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 4 cut(s) 674, 767, 830, 845
HspAI GCGC 1 cut(s) 405
KpnI GGTACC 1 cut(s) 538
Kzo9I GATC 3 cut(s) 282, 290, 444
LmnI GCTCC 2 cut(s) 313, 587
LweI GCATC 3 cut(s) 517, 627, 776
MaeI CTAG 3 cut(s) 317, 719, 851
MaeII ACGT 3 cut(s) 486, 507, 809
MaeIII GTNAC 1 cut(s) 665
MalI GATC 3 cut(s) 284, 292, 446
MboI GATC 3 cut(s) 282, 290, 444
MboII GAAGA 4 cut(s) 106, 362, 509, 553
MfeI CAATTG 1 cut(s) 433
MlsI TGGCCA 1 cut(s) 825
MluCI AATT 2 cut(s) 38, 433
MluNI TGGCCA 1 cut(s) 825
MlyI GAGTC 2 cut(s) 194, 561
MmeI TCCRAC 2 cut(s) 291, 460
MnlI CCTC 9 cut(s) 158, 162, 166, 219, 418, 578, 656, 813, 839
Mox20I TGGCCA 1 cut(s) 825
MroXI GAANNNNTTC 1 cut(s) 798
MscI TGGCCA 1 cut(s) 825
MseI TTAA 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 825
MspI CCGG 2 cut(s) 160, 263
MspR9I CCNGG 5 cut(s) 160, 263, 287, 359, 782
MunI CAATTG 1 cut(s) 433
MvaI CCWGG 3 cut(s) 287, 359, 782
MwoI GCNNNNNNNGC 4 cut(s) 278, 313, 404, 786
NciI CCSGG 2 cut(s) 160, 263
NdeII GATC 3 cut(s) 282, 290, 444
NheI GCTAGC 1 cut(s) 850
NlaIII CATG 4 cut(s) 674, 767, 830, 845
NlaIV GGNNCC 2 cut(s) 338, 536
NspI RCATGY 1 cut(s) 767
PciI ACATGT 1 cut(s) 763
PcsI WCGNNNNNNNCGW 2 cut(s) 305, 483
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 7 cut(s) 173, 179, 349, 389, 415, 547, 674
PfoI TCCNGGA 1 cut(s) 261
PkrI GCNGC 3 cut(s) 303, 306, 458
PleI GAGTC 2 cut(s) 193, 560
PpsI GAGTC 2 cut(s) 193, 560
PscI ACATGT 1 cut(s) 763
PsiI TTATAA 1 cut(s) 818
Psp6I CCWGG 3 cut(s) 285, 357, 780
PspEI GGTNACC 1 cut(s) 665
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 3 cut(s) 285, 357, 780
PspN4I GGNNCC 2 cut(s) 338, 536
PspPI GGNCC 2 cut(s) 336, 458
RsaI GTAC 3 cut(s) 536, 683, 688
RsaNI GTAC 3 cut(s) 535, 682, 687
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 3 cut(s) 302, 305, 457
Sau3AI GATC 3 cut(s) 282, 290, 444
Sau96I GGNCC 2 cut(s) 336, 458
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 2 cut(s) 194, 561
ScrFI CCNGG 5 cut(s) 160, 263, 287, 359, 782
SfaNI GCATC 3 cut(s) 517, 627, 776
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse9I AATT 2 cut(s) 38, 433
SsiI CCGC 5 cut(s) 212, 302, 305, 456, 471
SspI AATATT 1 cut(s) 88
SspMI CTAG 3 cut(s) 317, 719, 851
StyD4I CCNGG 5 cut(s) 158, 261, 285, 357, 780
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 2 cut(s) 203, 686
TaiI ACGT 3 cut(s) 489, 510, 812
TaqI TCGA 4 cut(s) 330, 387, 447, 550
TasI AATT 2 cut(s) 38, 433
TatI WGTACW 2 cut(s) 681, 686
TauI GCSGC 3 cut(s) 304, 307, 459
TfiI GAWTC 7 cut(s) 173, 179, 349, 389, 415, 547, 674
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 687
TspRI CASTG 1 cut(s) 206
XapI RAATTY 1 cut(s) 38
XceI RCATGY 1 cut(s) 767
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 3 cut(s) 317, 719, 851
ZraI GACGTC 1 cut(s) 487
ZrmI AGTACT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.