RchiOBHm_Chr4g0426431

Ubiquitin-2 like Rad60 SUMO-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003492
Physical Location & Seq
Forward (+)
51915364 .. 51915828
465 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39548

Sequence Viewer

Length: 465 bp
ATGCAAATTTTCGTGAAAACTCTAACTGGGAAGACCATAACTCTCGAGGTTGAGGGTAGCGATACCATCGACAATGTCAAAGCCAAGATCCAGGATAAAGAAGGCATCCCTCCTGATCAACAGAGACTGATCTTTGCTGGCAAGCAATTGGAAGATGGCAGAACTCTTGCGGATTATAACATTCAAAAGGAGTCGACTCTTCACCTGGTGTTGAGGCTGAGGGGAGGAACTATGATCAAGGTGAAGACTCTCACTGGGAAAGAAATTGAGATTGATATTGAATTGACTGATACCATTGACCGAATCAAGGAAAGGGTTGAGGAGAAAGAGGGGATTCCTCCGCTGCAACAAAGGCTGATTTACGGTGGCAAGCAGCTTGGAGATGACAAAACTGCTAAAGAATACAAAATCGAGGGTGGCTCTGTCCTTCATCTTGTACTTGCATTACGTGGTGGTAGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

154

Amino Acids

17.2

Weight (kDa)

6.17

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 4.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 5.1e-32 Ubiquitin family
ubiquitin PF00240 79 - 149 6.9e-27 Ubiquitin family
Rad60-SLD PF11976 79 - 147 1.5e-14 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccI GTMKAC 1 cut(s) 194
AciI CCGC 2 cut(s) 170, 341
AclWI GGATC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 6
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 2 cut(s) 185, 281
AjnI CCWGG 2 cut(s) 90, 204
AluBI AGCT 1 cut(s) 376
AluI AGCT 1 cut(s) 376
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 82
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 44
ApeKI GCWGC 2 cut(s) 343, 373
ApoI RAATTY 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 194, 253
AvaI CYCGRG 1 cut(s) 44
BbsI GAAGAC 2 cut(s) 38, 251
BbvCI CCTCAGC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 330, 385
BccI CCATC 2 cut(s) 74, 149
BciT130I CCWGG 2 cut(s) 92, 206
BclI TGATCA 2 cut(s) 115, 234
BcoDI GTCTC 1 cut(s) 118
BisI GCNGC 2 cut(s) 344, 374
BlsI GCNGC 2 cut(s) 345, 375
Bme1390I CCNGG 2 cut(s) 92, 206
BmeT110I CYCGRG 1 cut(s) 44
BmrFI CCNGG 2 cut(s) 92, 206
BmrI ACTGGG 2 cut(s) 36, 264
BmsI GCATC 1 cut(s) 114
BmuI ACTGGG 2 cut(s) 36, 264
BpiI GAAGAC 2 cut(s) 38, 251
BplI GAGNNNNNCTC 2 cut(s) 404, 436
Bpu10I CCTNAGC 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 449
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 31, 259
BseBI CCWGG 2 cut(s) 92, 206
BseGI GGATG 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 2 cut(s) 31, 259
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 2 cut(s) 330, 385
BsiHKCI CYCGRG 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 118
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 4 cut(s) 87, 115, 129, 234
BspACI CCGC 2 cut(s) 170, 341
BspCNI CTCAG 1 cut(s) 210
BspPI GGATC 1 cut(s) 82
BsrI ACTGG 2 cut(s) 31, 259
BssMI GATC 4 cut(s) 87, 115, 129, 234
Bst2UI CCWGG 2 cut(s) 92, 206
Bst4CI ACNGT 1 cut(s) 365
Bst6I CTCTTC 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 449
BstC8I GCNNGC 3 cut(s) 139, 143, 371
BstDEI CTNAG 1 cut(s) 218
BstF5I GGATG 1 cut(s) 105
BstKTI GATC 4 cut(s) 90, 118, 132, 237
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 4 cut(s) 87, 115, 129, 234
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNI CCWGG 2 cut(s) 92, 206
BstSCI CCNGG 2 cut(s) 90, 204
BstV1I GCAGC 2 cut(s) 330, 385
BstV2I GAAGAC 2 cut(s) 38, 251
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BtsCI GGATG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 139, 143, 371
CaiI CAGNNNCTG 1 cut(s) 127
CsiI ACCWGGT 1 cut(s) 204
Csp6I GTAC 1 cut(s) 437
CviJI RGCY 5 cut(s) 83, 217, 355, 376, 420
CviKI_1 RGCY 5 cut(s) 83, 217, 355, 376, 420
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 1 cut(s) 218
DpnI GATC 4 cut(s) 89, 117, 131, 236
DpnII GATC 4 cut(s) 87, 115, 129, 234
DraIII CACNNNGTG 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 90, 204
FaiI YATR 3 cut(s) 38, 177, 233
FbaI TGATCA 2 cut(s) 115, 234
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 344, 374
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 2 cut(s) 344, 374
GluI GCNGC 2 cut(s) 344, 374
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 5 cut(s) 191, 196, 247, 303, 334
HphI GGTGA 2 cut(s) 194, 253
Hpy166II GTNNAC 1 cut(s) 195
Hpy188III TCNNGA 3 cut(s) 13, 44, 113
Hpy8I GTNNAC 1 cut(s) 195
HpyAV CCTTC 2 cut(s) 95, 437
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 1 cut(s) 448
HpyCH4V TGCA 3 cut(s) 4, 346, 443
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 1 cut(s) 448
Ksp22I TGATCA 2 cut(s) 115, 234
Kzo9I GATC 4 cut(s) 87, 115, 129, 234
LpnPI CCDG 8 cut(s) 12, 77, 104, 123, 126, 191, 218, 240
Lsp1109I GCAGC 2 cut(s) 330, 385
LweI GCATC 1 cut(s) 114
MabI ACCWGGT 1 cut(s) 204
MaeII ACGT 1 cut(s) 448
MalI GATC 4 cut(s) 89, 117, 131, 236
MboI GATC 4 cut(s) 87, 115, 129, 234
MboII GAAGA 4 cut(s) 43, 164, 191, 256
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 1 cut(s) 87
MluCI AATT 4 cut(s) 6, 146, 264, 281
MlyI GAGTC 3 cut(s) 190, 200, 241
MseI TTAA 1 cut(s) 463
MspA1I CMGCKG 1 cut(s) 343
MspR9I CCNGG 2 cut(s) 92, 206
MunI CAATTG 1 cut(s) 146
MvaI CCWGG 2 cut(s) 92, 206
MwoI GCNNNNNNNGC 1 cut(s) 352
NdeII GATC 4 cut(s) 87, 115, 129, 234
PaeR7I CTCGAG 1 cut(s) 44
PfeI GAWTC 2 cut(s) 303, 334
PflFI GACNNNGTC 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 2 cut(s) 345, 375
PleI GAGTC 3 cut(s) 190, 199, 241
PpsI GAGTC 3 cut(s) 190, 199, 241
Ppu21I YACGTR 1 cut(s) 449
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 90, 204
PspGI CCWGG 2 cut(s) 90, 204
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 87
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
SalI GTCGAC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 463
SatI GCNGC 2 cut(s) 344, 374
Sau3AI GATC 4 cut(s) 87, 115, 129, 234
SchI GAGTC 3 cut(s) 190, 200, 241
ScrFI CCNGG 2 cut(s) 92, 206
SetI ASST 5 cut(s) 51, 207, 243, 378, 451
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 4 cut(s) 6, 146, 264, 281
SsiI CCGC 2 cut(s) 170, 341
StyD4I CCNGG 2 cut(s) 90, 204
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 1 cut(s) 451
TaqI TCGA 4 cut(s) 45, 69, 194, 411
TaqII GACCGA 1 cut(s) 315
TasI AATT 4 cut(s) 6, 146, 264, 281
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 2 cut(s) 303, 334
Tru1I TTAA 1 cut(s) 463
Tru9I TTAA 1 cut(s) 463
TscAI CASTG 1 cut(s) 259
TseI GCWGC 2 cut(s) 343, 373
TspDTI ATGAA 1 cut(s) 419
TspRI CASTG 1 cut(s) 259
Tth111I GACNNNGTC 1 cut(s) 74
XapI RAATTY 1 cut(s) 6
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.