Rorug05G0088500

ubiquitin-NEDD8-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003492
Physical Location & Seq
Forward (+)
7613603 .. 7614972
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0088500.1

Sequence Viewer

Length: 1179 bp
ATGAAGTGGGGTAGTGGTAGCAAAACCAAAAAGCCTCCACCTTCCTTTTCTGCTTCTTCTTCTTCTTCTTCTTCTACTTCTAAGCCTTCTTTGATCTCCCATGTCTTTCCAAGTTCATGGCTTTCGAAGTTCAAGCAAAAGACTGGCAATTCAGAGTCGAAACCCAGTAAAGTAAATCGAAAAGGGAAGCGGAATTCTCCATCTTTGGGTTCACCAAGGATTGCTAGTGGGGGTGGTGGCAGATTCTATGGTGATGATGATGGTGTTGATGGATATTGGAGGCTGTCTTTTGGTGAGGATAGTGTTGAAGGAGAGAAGAAGAGTAGGGGTGCATTACGATCGGTTCGGTATGAATCCGATGAAGAATTTGAAGCTAAGCCCTCGAAGGTGATAGGCATGAGTAAAAGTGGGAATGCTATAGAGTGGAAGTTGAGGAAAGGAAATAAAGGAGTTTCAGGGAAGAAGGGGTTAGAATTAGAAAGAGAAGCAGAGGAATTAGAAGAGAGATCAAGCAAAACAGGCGAAGAGGATGATGCACTGGAAATGTTAGCAGCAAGAACAATGTTGACAAAGGAGAAGGATAGTAAGCATGGATTGGCTTCTGGTGCAAGGAAATATCATTATGCATCTTCGAATCTCAAAACAATTGAAGAAGAAGGTTCTGAAGAAAAGCCGACTTCAGATTGGCAAAAATTGAAGGAAAGGAAAATACAAGAGGTGATGGAAAAGAGTGAGAAACATAGGAAATCTCTCTACATAAGCAGGGACTTACAGAGGAAACCAAGACCAAAGCGGAGTAACAGAGTCAGAGTTTGCTCTCCAAGAACAGCTTCAAGAGTTGAAATCTGCAAAATCAAAGCTCTCCAAGAGATGGCGAAAGCGAAAAAGGCAGCTAAGGAGAGAAAGGTGCAGCAGGTGAGAACAGGCTTGGATAGCTTTGCAGTGGTGAAGTGCTCATTCGACCCGCAACAGGACTTCAGAGATTCCATGGTTGAGATGATTGTGGAGAAAAAGATTACCCGGCCAGACGATCTTGAAGAGCTCTTAGCTTGTTACCTGACACTGAATTCCGATGAATACCATGATCTCATCATCAAGGTATTCCGGCAGGTATGGTTTGATTTGAATCAAACCTGTTTTGGTAGTGAACTACAGAATGATCAATGTTGCAATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

392

Amino Acids

44.22

Weight (kDa)

9.34

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 319 - 375 1.7e-25 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 904
Acc36I ACCTGC 2 cut(s) 904, 1099
AccB7I CCANNNNNTGG 1 cut(s) 871
AciI CCGC 3 cut(s) 190, 793, 965
AcoI YGGCCR 1 cut(s) 1022
AcsI RAATTY 3 cut(s) 193, 365, 1066
AcuI CTGAAG 3 cut(s) 663, 684, 961
AfiI CCNNNNNNNGG 3 cut(s) 206, 871, 970
AgsI TTSAA 9 cut(s) 133, 308, 371, 650, 697, 834, 842, 1037, 1126
AjuI GAANNNNNNNTTGG 2 cut(s) 814, 846
AluBI AGCT 7 cut(s) 374, 830, 860, 893, 936, 1042, 1049
AluI AGCT 7 cut(s) 374, 830, 860, 893, 936, 1042, 1049
Alw21I GWGCWC 2 cut(s) 956, 1044
AoxI GGCC 1 cut(s) 1022
ApeKI GCWGC 3 cut(s) 551, 890, 910
ApoI RAATTY 3 cut(s) 193, 365, 1066
Asp700I GAANNNNTTC 1 cut(s) 829
AsuC2I CCSGG 1 cut(s) 1021
AsuHPI GGTGA 7 cut(s) 204, 263, 305, 400, 730, 928, 958
AsuII TTCGAA 2 cut(s) 125, 632
BanII GRGCYC 1 cut(s) 1044
Bbv12I GWGCWC 2 cut(s) 956, 1044
BbvI GCAGC 3 cut(s) 563, 902, 922
BccI CCATC 5 cut(s) 208, 254, 263, 715, 865
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BclI TGATCA 1 cut(s) 1159
BcnI CCSGG 1 cut(s) 1021
BfaI CTAG 2 cut(s) 225, 1177
BfmI CTRYAG 2 cut(s) 417, 1151
BfuAI ACCTGC 2 cut(s) 904, 1099
BisI GCNGC 3 cut(s) 552, 891, 911
BlpI GCTNAGC 1 cut(s) 375
BlsI GCNGC 3 cut(s) 553, 892, 912
Bme1390I CCNGG 1 cut(s) 1021
BmrFI CCNGG 1 cut(s) 1021
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 2 cut(s) 523, 635
BmuI ACTGGG 1 cut(s) 159
Bpu10I CCTNAGC 1 cut(s) 894
Bpu1102I GCTNAGC 1 cut(s) 375
Bpu14I TTCGAA 2 cut(s) 125, 632
BpuMI CCSGG 1 cut(s) 1021
BsaBI GATNNNNATC 1 cut(s) 1125
BsaJI CCNNGG 2 cut(s) 215, 987
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 871, 970
Bse1I ACTGG 3 cut(s) 148, 165, 543
Bse3DI GCAATG 1 cut(s) 1177
Bse8I GATNNNNATC 1 cut(s) 1125
BseDI CCNNGG 2 cut(s) 215, 987
BseGI GGATG 1 cut(s) 535
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 3 cut(s) 206, 871, 970
BseMI GCAATG 1 cut(s) 1177
BseNI ACTGG 3 cut(s) 148, 165, 543
BseXI GCAGC 3 cut(s) 563, 902, 922
BsgI GTGCAG 1 cut(s) 929
Bsh1285I CGRYCG 1 cut(s) 341
BshFI GGCC 1 cut(s) 1024
BsiEI CGRYCG 1 cut(s) 341
BsiHKAI GWGCWC 2 cut(s) 956, 1044
BsiSI CCGG 2 cut(s) 1021, 1105
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 3 cut(s) 206, 871, 970
BsmFI GGGAC 1 cut(s) 779
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 1 cut(s) 1024
Bsp119I TTCGAA 2 cut(s) 125, 632
Bsp1286I GDGCHC 2 cut(s) 956, 1044
Bsp143I GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
Bsp1720I GCTNAGC 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 987
BspACI CCGC 3 cut(s) 190, 793, 965
BspANI GGCC 1 cut(s) 1024
BspMI ACCTGC 2 cut(s) 904, 1099
BspQI GCTCTTC 1 cut(s) 1032
BspT104I TTCGAA 2 cut(s) 125, 632
BsrDI GCAATG 1 cut(s) 1177
BsrI ACTGG 3 cut(s) 148, 165, 543
BssECI CCNNGG 2 cut(s) 215, 987
BssMI GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
BssT1I CCWWGG 2 cut(s) 215, 987
Bst6I CTCTTC 4 cut(s) 314, 495, 519, 1032
BstBI TTCGAA 2 cut(s) 125, 632
BstDEI CTNAG 4 cut(s) 81, 375, 894, 1045
BstDSI CCRYGG 1 cut(s) 987
BstF5I GGATG 1 cut(s) 535
BstKTI GATC 6 cut(s) 96, 341, 509, 1033, 1087, 1162
BstMBI GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
BstMCI CGRYCG 1 cut(s) 341
BstMWI GCNNNNNNNGC 4 cut(s) 519, 605, 887, 933
BstSCI CCNGG 1 cut(s) 1019
BstSFI CTRYAG 2 cut(s) 417, 1151
BstV1I GCAGC 3 cut(s) 563, 902, 922
BstXI CCANNNNNNTGG 1 cut(s) 117
BsuRI GGCC 1 cut(s) 1024
BtgI CCRYGG 1 cut(s) 987
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 3 cut(s) 536, 948, 1061
BveI ACCTGC 2 cut(s) 904, 1099
CviAII CATG 6 cut(s) 101, 117, 397, 590, 988, 1082
DdeI CTNAG 4 cut(s) 81, 375, 894, 1045
DpnI GATC 6 cut(s) 95, 340, 508, 1032, 1086, 1161
DpnII GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
EaeI YGGCCR 1 cut(s) 1022
Eam1104I CTCTTC 4 cut(s) 314, 495, 519, 1032
EarI CTCTTC 4 cut(s) 314, 495, 519, 1032
Ecl136II GAGCTC 1 cut(s) 1042
Eco130I CCWWGG 2 cut(s) 215, 987
Eco24I GRGCYC 1 cut(s) 1044
Eco53kI GAGCTC 1 cut(s) 1042
Eco57I CTGAAG 3 cut(s) 663, 684, 961
EcoICRI GAGCTC 1 cut(s) 1042
EcoRI GAATTC 2 cut(s) 193, 1066
EcoT14I CCWWGG 2 cut(s) 215, 987
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 1044
ErhI CCWWGG 2 cut(s) 215, 987
FaeI CATG 6 cut(s) 104, 120, 400, 593, 991, 1085
FalI AAGNNNNNCTT 2 cut(s) 814, 846
FaqI GGGAC 1 cut(s) 779
FatI CATG 6 cut(s) 100, 116, 396, 589, 987, 1081
FauI CCCGC 1 cut(s) 972
FbaI TGATCA 1 cut(s) 1159
Fnu4HI GCNGC 3 cut(s) 552, 891, 911
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 1 cut(s) 1044
Fsp4HI GCNGC 3 cut(s) 552, 891, 911
FspBI CTAG 2 cut(s) 225, 1177
GluI GCNGC 3 cut(s) 552, 891, 911
HaeIII GGCC 1 cut(s) 1024
HapII CCGG 2 cut(s) 1021, 1105
Hin1II CATG 6 cut(s) 104, 120, 400, 593, 991, 1085
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HinfI GANTC 7 cut(s) 155, 243, 353, 634, 804, 983, 1126
HpaII CCGG 2 cut(s) 1021, 1105
HphI GGTGA 7 cut(s) 204, 263, 305, 400, 730, 928, 958
Hpy166II GTNNAC 3 cut(s) 212, 567, 1148
Hpy188I TCNGA 7 cut(s) 154, 358, 664, 682, 809, 980, 1072
Hpy188III TCNNGA 2 cut(s) 834, 1034
Hpy8I GTNNAC 3 cut(s) 212, 567, 1148
HpyAV CCTTC 8 cut(s) 51, 96, 302, 379, 457, 571, 650, 691
HpyCH4V TGCA 8 cut(s) 332, 536, 608, 626, 849, 910, 941, 1170
HpyF10VI GCNNNNNNNGC 4 cut(s) 519, 605, 887, 933
HpyF3I CTNAG 4 cut(s) 81, 375, 894, 1045
Hsp92II CATG 6 cut(s) 104, 120, 400, 593, 991, 1085
Ksp22I TGATCA 1 cut(s) 1159
Kzo9I GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
LguI GCTCTTC 1 cut(s) 1032
Lsp1109I GCAGC 3 cut(s) 563, 902, 922
LweI GCATC 2 cut(s) 523, 635
MaeI CTAG 2 cut(s) 225, 1177
MaeIII GTNAC 2 cut(s) 797, 1052
MalI GATC 6 cut(s) 95, 340, 508, 1032, 1086, 1161
MboI GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 2 cut(s) 956, 1044
MluCI AATT 8 cut(s) 148, 193, 365, 473, 494, 645, 692, 1066
MlyI GAGTC 2 cut(s) 164, 813
MnlI CCTC 9 cut(s) 45, 273, 289, 391, 426, 484, 520, 709, 768
Mph1103I ATGCAT 1 cut(s) 628
MroXI GAANNNNTTC 1 cut(s) 829
MspI CCGG 2 cut(s) 1021, 1105
MspR9I CCNGG 1 cut(s) 1021
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 1 cut(s) 418
MwoI GCNNNNNNNGC 4 cut(s) 519, 605, 887, 933
NciI CCSGG 1 cut(s) 1021
NcoI CCATGG 1 cut(s) 987
NdeII GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
NlaIII CATG 6 cut(s) 104, 120, 400, 593, 991, 1085
NsiI ATGCAT 1 cut(s) 628
NspV TTCGAA 2 cut(s) 125, 632
PaqCI CACCTGC 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 1032
PctI GAATGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 829
PfeI GAWTC 5 cut(s) 243, 353, 634, 983, 1126
PflMI CCANNNNNTGG 1 cut(s) 871
PkrI GCNGC 3 cut(s) 553, 892, 912
Ple19I CGATCG 1 cut(s) 341
PleI GAGTC 2 cut(s) 163, 812
PpsI GAGTC 2 cut(s) 163, 812
Psp124BI GAGCTC 1 cut(s) 1044
PvuI CGATCG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 1044
SapI GCTCTTC 1 cut(s) 1032
SatI GCNGC 3 cut(s) 552, 891, 911
Sau3AI GATC 6 cut(s) 93, 338, 506, 1030, 1084, 1159
SchI GAGTC 2 cut(s) 164, 813
ScrFI CCNGG 1 cut(s) 1021
SduI GDGCHC 2 cut(s) 956, 1044
SfaNI GCATC 2 cut(s) 523, 635
SfcI CTRYAG 2 cut(s) 417, 1151
SfuI TTCGAA 2 cut(s) 125, 632
Sse9I AATT 8 cut(s) 148, 193, 365, 473, 494, 645, 692, 1066
SsiI CCGC 3 cut(s) 190, 793, 965
SspMI CTAG 2 cut(s) 225, 1177
SstI GAGCTC 1 cut(s) 1044
StyD4I CCNGG 1 cut(s) 1019
StyI CCWWGG 2 cut(s) 215, 987
TaqI TCGA 6 cut(s) 125, 158, 178, 383, 632, 960
TasI AATT 8 cut(s) 148, 193, 365, 473, 494, 645, 692, 1066
TfiI GAWTC 5 cut(s) 243, 353, 634, 983, 1126
TscAI CASTG 3 cut(s) 543, 948, 1068
TseI GCWGC 3 cut(s) 551, 890, 910
TspDTI ATGAA 5 cut(s) 17, 105, 366, 375, 1089
TspRI CASTG 3 cut(s) 543, 948, 1068
Van91I CCANNNNNTGG 1 cut(s) 871
XapI RAATTY 3 cut(s) 193, 365, 1066
XmnI GAANNNNTTC 1 cut(s) 829
XspI CTAG 2 cut(s) 225, 1177
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.