RchiOBHm_Chr4g0433581

GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003624
Physical Location & Seq
Reverse (-)
57664978 .. 57665733
756 bp
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UTR
Exon/CDS
Intron
PRQ40207

Sequence Viewer

Length: 555 bp
ATGCTGATATTAGTTTTCAACACTGCCCACTGGTGGAATTACTGGAAAACAGCTAGAGGACTACACTATTACCAAGAAGGCAACTATGTGCACCCAAGGCTAAAGGCTCTTGAAGCATACAAGAGAGCTCTCACCACTTGGGCTAGATGGGTGGACATGAACATCAATCCCAACCGGACTCGGGTCTTCTTCCGAGGATATGCATATTCCCATTACAGTAATGGGCAATGGAACTCAGGAGGACATTGCCTCAAAGAAACAGAACCAATCTTTAACGAAGCTCATTTATCGAAGTATCCTTCGAAAATGAGAGCTGTAGAGTCTGTACTTCAAGGAATGAAAACTCCGGTGACGTATTTTAACATAAGTAAGCTTACAGATTGCAGAAAAGATGCCCACCCTTCCATTTACAGAATGGAATACAAGACAAAAGAAGAAGAAATTGCTGCTGAACGATCTCAGGATTGCAACCATTGGTGCTTGCCGGGTGTTCCGGATACTTGGAACGAATTGCTATATGCCTCGTTTTTGAAGGACGGATGGGAATCAGGCTAG

Protein Analysis

184

Amino Acids

21.77

Weight (kDa)

8.8

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 3 - 174 1.8e-57 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 493
AfaI GTAC 1 cut(s) 327
AfiI CCNNNNNNNGG 2 cut(s) 33, 181
AgsI TTSAA 4 cut(s) 19, 113, 332, 532
AluBI AGCT 5 cut(s) 53, 128, 281, 314, 373
AluI AGCT 5 cut(s) 53, 128, 281, 314, 373
Alw21I GWGCWC 2 cut(s) 93, 130
Alw44I GTGCAC 1 cut(s) 89
Ama87I CYCGRG 1 cut(s) 180
Aor13HI TCCGGA 1 cut(s) 493
ApaLI GTGCAC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 2 cut(s) 124, 361
AsuII TTCGAA 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 93
BanII GRGCYC 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 178
Bbv12I GWGCWC 2 cut(s) 93, 130
BbvI GCAGC 1 cut(s) 433
BccI CCATC 2 cut(s) 141, 534
BciVI GTATCC 2 cut(s) 306, 490
BcnI CCSGG 1 cut(s) 486
BfaI CTAG 3 cut(s) 54, 144, 553
BfmI CTRYAG 1 cut(s) 315
BfuI GTATCC 2 cut(s) 306, 490
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 1 cut(s) 486
BmeT110I CYCGRG 1 cut(s) 180
BmrFI CCNGG 1 cut(s) 486
BmsI GCATC 1 cut(s) 382
BoxI GACNNNNGTC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 178
Bpu14I TTCGAA 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 95, 193
BsaWI WCCGGW 3 cut(s) 174, 346, 493
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 181
Bse1I ACTGG 2 cut(s) 35, 47
Bse3DI GCAATG 2 cut(s) 233, 244
Bse8I GATNNNNATC 1 cut(s) 544
BseAI TCCGGA 1 cut(s) 493
BseDI CCNNGG 2 cut(s) 95, 193
BseGI GGATG 1 cut(s) 545
BseJI GATNNNNATC 1 cut(s) 544
BseLI CCNNNNNNNGG 2 cut(s) 33, 181
BseMI GCAATG 2 cut(s) 233, 244
BseMII CTCAG 2 cut(s) 249, 473
BseNI ACTGG 2 cut(s) 35, 47
BseSI GKGCMC 1 cut(s) 93
BseXI GCAGC 1 cut(s) 433
BsiHKAI GWGCWC 2 cut(s) 93, 130
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 4 cut(s) 175, 347, 485, 494
BslI CCNNNNNNNGG 2 cut(s) 33, 181
BsoBI CYCGRG 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 93, 130
Bsp13I TCCGGA 1 cut(s) 493
Bsp143I GATC 1 cut(s) 455
BspCNI CTCAG 2 cut(s) 248, 472
BspEI TCCGGA 1 cut(s) 493
BspT104I TTCGAA 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 233, 244
BsrI ACTGG 2 cut(s) 35, 47
BssECI CCNNGG 2 cut(s) 95, 193
BssMI GATC 1 cut(s) 455
BssT1I CCWWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 482
BstDEI CTNAG 2 cut(s) 235, 459
BstF5I GGATG 1 cut(s) 545
BstKTI GATC 1 cut(s) 458
BstMBI GATC 1 cut(s) 455
BstMWI GCNNNNNNNGC 2 cut(s) 97, 113
BstPAI GACNNNNGTC 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 178
BsuI GTATCC 2 cut(s) 306, 490
BtsCI GGATG 1 cut(s) 545
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 21, 28
Cac8I GCNNGC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 326
CviAII CATG 1 cut(s) 157
CviJI RGCY 9 cut(s) 53, 100, 107, 128, 143, 281, 314, 373, 552
CviKI_1 RGCY 9 cut(s) 53, 100, 107, 128, 143, 281, 314, 373, 552
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 2 cut(s) 235, 459
DpnI GATC 1 cut(s) 457
DpnII GATC 1 cut(s) 455
Ecl136II GAGCTC 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 95
EcoT22I ATGCAT 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 1 cut(s) 160
FaiI YATR 8 cut(s) 87, 118, 158, 201, 205, 365, 517, 519
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 447
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 3 cut(s) 54, 144, 553
GluI GCNGC 1 cut(s) 447
HapII CCGG 4 cut(s) 175, 347, 485, 494
Hin1II CATG 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 3 cut(s) 178, 320, 545
HpaII CCGG 4 cut(s) 175, 347, 485, 494
HphI GGTGA 2 cut(s) 124, 361
Hpy166II GTNNAC 2 cut(s) 91, 154
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 4 cut(s) 110, 237, 461, 494
Hpy8I GTNNAC 2 cut(s) 91, 154
HpyAV CCTTC 4 cut(s) 71, 309, 411, 526
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 4 cut(s) 91, 203, 384, 468
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 113
HpyF3I CTNAG 2 cut(s) 235, 459
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 1 cut(s) 160
Kpn2I TCCGGA 1 cut(s) 493
Kzo9I GATC 1 cut(s) 455
LpnPI CCDG 9 cut(s) 16, 28, 188, 222, 360, 446, 498, 507, 534
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 1 cut(s) 382
MaeI CTAG 3 cut(s) 54, 144, 553
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 349
MalI GATC 1 cut(s) 457
MboI GATC 1 cut(s) 455
MboII GAAGA 4 cut(s) 178, 181, 446, 449
MhlI GDGCHC 2 cut(s) 93, 130
MluCI AATT 3 cut(s) 37, 441, 509
MlyI GAGTC 2 cut(s) 172, 329
MnlI CCTC 5 cut(s) 50, 188, 233, 260, 532
Mph1103I ATGCAT 1 cut(s) 205
MroI TCCGGA 1 cut(s) 493
MseI TTAA 2 cut(s) 273, 360
MspI CCGG 4 cut(s) 175, 347, 485, 494
MspR9I CCNGG 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 97, 113
NciI CCSGG 1 cut(s) 486
NdeII GATC 1 cut(s) 455
NlaIII CATG 1 cut(s) 160
NmuCI GTSAC 1 cut(s) 349
NsiI ATGCAT 1 cut(s) 205
NspV TTCGAA 1 cut(s) 302
PfeI GAWTC 1 cut(s) 545
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 2 cut(s) 172, 328
PpsI GAGTC 2 cut(s) 172, 328
PshAI GACNNNNGTC 1 cut(s) 182
Psp124BI GAGCTC 1 cut(s) 130
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
SacI GAGCTC 1 cut(s) 130
SaqAI TTAA 2 cut(s) 273, 360
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 1 cut(s) 455
SchI GAGTC 2 cut(s) 172, 329
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 2 cut(s) 93, 130
SetI ASST 6 cut(s) 55, 130, 283, 316, 356, 375
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 1 cut(s) 315
SfuI TTCGAA 1 cut(s) 302
Sse9I AATT 3 cut(s) 37, 441, 509
SspMI CTAG 3 cut(s) 54, 144, 553
SstI GAGCTC 1 cut(s) 130
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 218
TaiI ACGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 290, 302
TasI AATT 3 cut(s) 37, 441, 509
TatI WGTACW 1 cut(s) 325
TfiI GAWTC 1 cut(s) 545
Tru1I TTAA 2 cut(s) 273, 360
Tru9I TTAA 2 cut(s) 273, 360
TscAI CASTG 2 cut(s) 28, 35
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 2 cut(s) 173, 353
TspGWI ACGGA 1 cut(s) 552
TspRI CASTG 2 cut(s) 28, 35
VneI GTGCAC 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 2 cut(s) 218, 412
XspI CTAG 3 cut(s) 54, 144, 553
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.