Rh4CG418300

GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003624
Physical Location & Seq
Forward (+)
66303956 .. 66314987
11032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG418300.1

Sequence Viewer

Length: 3246 bp
ATGAACAGAGAGAAAAGAGTTTTTATCAATATGGGAACAATCAGTGGTTTCTTGATGTTGTTGTGTAAGGTGGGTTTACTTGTTTTGGTATTGGCAGAGGCTTCTTCTGCAACTCTCTCTCCTACTGGTGTAAATTATGAAGTTGAAGCTTTGGTGGCCATAAAAAGCGATCTAATTGACCCACACAATGTTTTGGAGAACTGGGATAGTAACTCTGTAGATCCTTGTAGCTGGAGGATGGTTACTTGCACACCTGATGGCTATGTATCTGCTCTGGACCTTTCTTTCAACAATCTCAGTGGCTCCTTGCCAAAAGTATCTGCAAGAACTTTCAAAATTGTTGGTAACCCTTTGATTTGTGGCCTGAAGGCTGCGAACAACTGTTCTGCTGTCTTGCCGGAGCCATTATCTTTCCCACCTGATGCTCTAAACGCTCAATCAGACTCTGGGTCGCAACGCCACCACTTGACTGTAGTCTTTGCTGCAAGCTTTGGTGCTGCCTTTGCTGTTATAATAATAATTGGGTTACTTGTTTGGTTAAGATATAGACACAACCAGCAGATATTCTTCGACGTCAATGATCAATATGACCCTGAAGTATGCTTGGGTCATTTGAAAAGGTATACATTTAAGGAGCTTCGGACAGCAACAGACCATTTTAACTCTAAGAACATTCTTGGAAGAGGTGGCTTTGGGATTGTATTCAAGGGATGTTTGAATGATGGGACTCTGGTGGCGGTTAAGAGGCTGAAGGATTACAACACTGCTGGTGGTGAAATTCAGTTTCAGACAGAAGTTGAGATGATAAGTTTGGCAGTCCATCGGAATCTTCTGAGGCTTTGTGGGTTTTGTTCAACTGAGAATGAAAGACTTCTTGTTTACCCTTACATGCCTAATGGAAGCGTAGCATCTCGACTAAGAGATCATATTCATGGTCGGCCAGCTATAGACTGGGCAAGGAGAAAAAGAATAGCCTTGGGTCAAAAGGCTTTGGATTTTGGAAGAGTTGCAAACCAGAAGGGTGTAATGCTTGATTGGGTTAAGAAGCTCCATCAGGAGGGAAAGCTAAATCTGATGGGGGTTGAGACACCAAGGTTCCGGTCATGTGAACACCCTCATCAAAGATATTCAGATTTTATAGATGAATCTTCACTTGTGGTAGAAGCTATGGAGCTTTCTGGTCCTAGGTTTGCACGGAGAGATTCTAGACTTGGAGTAAAGCAGAGAGCAGAAGATGAAGGTCCAGCCGTCCAGGAGGTTAAGGGCCGGCCACGTGTCCGGCTATGTGTCGGCGGGAACCCAATTCGTATTTGTGTCGCTCTTCGGTTCCGGACAGTTAATAAAGAAGGAGACGAAAGCCTCACCAATCTGACCAACGTACCCTTCACAAAAGCAAAAGCCATGGATTCCAAGAAGCTAGAGCAGTTTTTCTCACCCAGAAGAAAAGTGGTTTCTGGGTTTAGTTTGGGAATTGGAGCCTCTCTGTTTGTAATCGCCGTACTATTTGTCTCTTACTCCTTAAAGAGTCCTACTGTGGACCAAGGTTTCTATACTGTAAATTCCTCTTTTGTTTCATGGCCTTTCTCGTTTTCAAGCACTGCAAATTCTGTTACTTCAGAAATGCAAGACTCTGCGGACTTGAACAAGACCCAATTGGGGAATTTCACAGAGGAAGTTAAAGATGGAAGCTCTGGGGTTGAAGGAGGAAGGGTGGAGCATAAAGATGGTGAGTTTGGTTCAAATGAGGGAGGGGGTGTTTTGGGGATTTTCCATTTGGGGAATTCCTCTGAGATTTTGAATAATGGAAGCTTGCATGTTGAAGGTGGAGGTGGGAAAGAGACAGTTTTGGAGAAGTCCCAGATGGGGAATTTTACAGAGGCAGTTAAAGATGGAAGCTTTGTGGTTGAGGAAGGAAGAGTTCAACAGAAAGATAATAACTCAGATTCAAGAAATTCCTCTGAGATTTTGAAATTTGAGAGCTTGCATGGTGAAGGTGGGAAGGGAATTGGGAATTTAAGCCTTTCTGGGAAAGAAGATGTGCATGCAGAGAAAACAGTTGAAGGTAGTTTTTCAAAGAATTCTAGTAATGGGGATGGTAAGGTAACAGAAGATCATCAAGGAAATTCAACACAGAAAAATGAAATCATTGAGCTTGATTCTCAAACTTGGGAAATGCCTAGTGACTCTGATAAAAAATGTGATATTTTTGATGGTAAATGGGTGAGAGATGATTCAAAGCCGTACTATCCTGGAGGTTCTTGTCCACACGTTGATAAGGTTTGGAATTGCCACTCCAATCGAAGGCCAGACAGTGCTTTCCTAAAATGGAAATGGCAGCCAAATGGGTGTGATATCCCAAGTTTGAATGCAACTGATTTTCTGGAGAGGTTGAGAGGAAAGAGGTTGGTTTTTGTGGGGGATTCACTGAATAGGAACATGTGGGAGTCTCTTGTGTGCATTCTCCGTCACAGCGTTCCGCACAAGAAAAAGGTATATGAGATTTCGGGAAAGAAGGAATTTAAGAAGAAGGGGATATATGCATTCAGATTTGAGGAGTACAATTGTTCCCTGGATTTTGTGGTTGCTCCATTCCTTGTTAGGGAGTCAACATTCACAGGCAAAAACTGTTCGTTTGAGACATTGAGGTTGGATTTGATGGACCGGACTACAACAACGTATCGTGATGCTGATGTTATAGTTTTTAATACTGGTCATTGGTGGACTCATGATAAAACTTCTAGAGGAGAAGACTATTACCAAGAAGGCAACTATGTGCATCCAAGGCTCAAGGCTCTTGAAGCATACAAGAGAGCTCTCACCACTTGGGCGAGATGGGTGGACAGATACATCAATGCCAACAGGACTCGGGTTTTCTTCAGAGGATATTCAGTCACCCATTTCAGTGGAGGGCAATGGAACTCAGGTGGACAGTGCCACAAAGAAACAGAACCAATCTTTAATGAGACTTATCTAACGCATTACCCTTCGAAAATGAGAGCTCTAGAGAGTGTGCTTAAAGAAATGAAAACTCCAGTGACATATCTTAACATTAGTAGGCTTACGGATTATAGAAAAGACGGACACCCTTCCATTTACAGAACGATATACAAGACAAAGGAAGAACAAATTACAGCTGAACAATCTCAGGATTGCAGTCATTGGTGCTTGCCTGGGGTTCCGGATACTTGGAATGAATTGTTATACGCTTTGCTGCTGCAGACCGGATGGGGATCAGGGCAAAAATGA

Protein Analysis

1081

Amino Acids

121.25

Weight (kDa)

8.26

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 45 - 84 2.6e-14 Leucine rich repeat N-terminal domain
Pkinase PF00069 221 - 309 3.3e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 223 - 321 1.5e-13 Protein tyrosine and serine/threonine kinase
PMR5N PF14416 731 - 784 1.6e-20 PMR5 N terminal Domain
PC-Esterase PF13839 785 - 1069 1.2e-100 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
AatII GACGTC 1 cut(s) 576
AccI GTMKAC 1 cut(s) 623
AccIII TCCGGA 2 cut(s) 1329, 3178
AciI CCGC 4 cut(s) 737, 1293, 1634, 2477
AclWI GGATC 2 cut(s) 215, 3238
AcoI YGGCCR 3 cut(s) 156, 938, 1268
AcuI CTGAAG 5 cut(s) 386, 615, 770, 1599, 2860
AcvI CACGTG 1 cut(s) 1274
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 4 cut(s) 1380, 1500, 2243, 2558
AfiI CCNNNNNNNGG 5 cut(s) 1057, 1656, 1863, 2301, 2599
AflIII ACRYGT 3 cut(s) 1273, 2266, 2436
AhdI GACNNNNNGTC 1 cut(s) 448
AjnI CCWGG 4 cut(s) 1251, 2248, 2568, 3169
AjuI GAANNNNNNNTTGG 2 cut(s) 1367, 1399
Alw21I GWGCWC 2 cut(s) 2815, 3001
Alw26I GTCTC 7 cut(s) 1079, 1344, 1513, 1832, 2451, 2630, 2957
AlwI GGATC 2 cut(s) 215, 3238
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 2865
Aor13HI TCCGGA 2 cut(s) 1329, 3178
AoxI GGCC 7 cut(s) 156, 361, 938, 1264, 1268, 1577, 2303
ApeKI GCWGC 6 cut(s) 371, 482, 497, 2335, 3211, 3214
Asp700I GAANNNNTTC 1 cut(s) 870
AspA2I CCTAGG 1 cut(s) 1184
AspS9I GGNCC 6 cut(s) 277, 1181, 1241, 1264, 1537, 2659
AsuHPI GGTGA 8 cut(s) 785, 1354, 1425, 1739, 2000, 2233, 2809, 2884
AsuII TTCGAA 1 cut(s) 2987
AvaI CYCGRG 1 cut(s) 2865
AvaII GGWCC 5 cut(s) 277, 1181, 1241, 1537, 2659
AvrII CCTAGG 1 cut(s) 1184
BaeI ACNNNNGTAYC 2 cut(s) 1362, 1395
BalI TGGCCA 1 cut(s) 158
BanII GRGCYC 2 cut(s) 2815, 3001
BbrPI CACGTG 1 cut(s) 1274
BbsI GAAGAC 1 cut(s) 2754
Bbv12I GWGCWC 2 cut(s) 2815, 3001
BbvI GCAGC 6 cut(s) 358, 469, 484, 2347, 3198, 3201
BceAI ACGGC 3 cut(s) 1232, 1481, 2224
BciT130I CCWGG 4 cut(s) 1253, 2250, 2570, 3171
BciVI GTATCC 1 cut(s) 3175
BclI TGATCA 1 cut(s) 580
BcoDI GTCTC 7 cut(s) 1079, 1344, 1513, 1832, 2451, 2630, 2957
BfaI CTAG 7 cut(s) 1185, 1206, 1418, 2082, 2178, 2739, 3002
BfmI CTRYAG 4 cut(s) 216, 471, 945, 3215
BfuI GTATCC 1 cut(s) 3175
BisI GCNGC 6 cut(s) 372, 483, 498, 2336, 3212, 3215
BlnI CCTAGG 1 cut(s) 1184
BlsI GCNGC 6 cut(s) 373, 484, 499, 2337, 3213, 3216
Bme1390I CCNGG 4 cut(s) 1253, 2250, 2570, 3171
Bme18I GGWCC 5 cut(s) 277, 1181, 1241, 1537, 2659
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 2865
BmgT120I GGNCC 6 cut(s) 277, 1181, 1241, 1264, 1537, 2659
BmiI GGNNCC 7 cut(s) 304, 402, 1097, 1298, 1328, 1477, 3177
BmrFI CCNGG 4 cut(s) 1253, 2250, 2570, 3171
BmrI ACTGGG 2 cut(s) 211, 961
BmsI GCATC 4 cut(s) 412, 917, 2674, 2785
BmuI ACTGGG 2 cut(s) 211, 961
BoxI GACNNNNGTC 1 cut(s) 473
BpiI GAAGAC 1 cut(s) 2754
BpmI CTGGAG 4 cut(s) 253, 2271, 2402, 3015
Bpu14I TTCGAA 1 cut(s) 2987
BpuEI CTTGAG 1 cut(s) 2771
BsaAI YACGTR 1 cut(s) 1274
BsaBI GATNNNNATC 1 cut(s) 3229
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 8 cut(s) 975, 1091, 1184, 1401, 1540, 2568, 2780, 3170
BsaWI WCCGGW 5 cut(s) 1098, 1329, 2661, 3178, 3221
BsaXI ACNNNNNCTCC 6 cut(s) 103, 133, 1695, 1725, 1740, 1770
Bsc4I CCNNNNNNNGG 5 cut(s) 1057, 1656, 1863, 2301, 2599
Bse118I RCCGGY 1 cut(s) 1266
Bse1I ACTGG 5 cut(s) 130, 206, 956, 2713, 3032
Bse3DI GCAATG 1 cut(s) 2918
Bse8I GATNNNNATC 1 cut(s) 3229
BseAI TCCGGA 2 cut(s) 1329, 3178
BseBI CCWGG 4 cut(s) 1253, 2250, 2570, 3171
BseDI CCNNGG 8 cut(s) 975, 1091, 1184, 1401, 1540, 2568, 2780, 3170
BseGI GGATG 5 cut(s) 243, 716, 2098, 2776, 3230
BseJI GATNNNNATC 1 cut(s) 3229
BseLI CCNNNNNNNGG 5 cut(s) 1057, 1656, 1863, 2301, 2599
BseMI GCAATG 1 cut(s) 2918
BseMII CTCAG 8 cut(s) 310, 824, 849, 1779, 1950, 1953, 2934, 3158
BseNI ACTGG 5 cut(s) 130, 206, 956, 2713, 3032
BseRI GAGGAG 2 cut(s) 2567, 2757
BseXI GCAGC 6 cut(s) 358, 469, 484, 2347, 3198, 3201
BshFI GGCC 7 cut(s) 158, 363, 940, 1266, 1270, 1579, 2305
BsiHKAI GWGCWC 2 cut(s) 2815, 3001
BsiHKCI CYCGRG 1 cut(s) 2865
BsiSI CCGG 8 cut(s) 398, 1099, 1267, 1279, 1330, 2662, 3179, 3222
BslFI GGGAC 2 cut(s) 739, 1840
BslI CCNNNNNNNGG 5 cut(s) 1057, 1656, 1863, 2301, 2599
BsmAI GTCTC 7 cut(s) 1079, 1344, 1513, 1832, 2451, 2630, 2957
BsmBI CGTCTC 1 cut(s) 1344
BsmFI GGGAC 2 cut(s) 739, 1840
BsmI GAATGC 3 cut(s) 2371, 2457, 2540
BsnI GGCC 7 cut(s) 158, 363, 940, 1266, 1270, 1579, 2305
BsoBI CYCGRG 1 cut(s) 2865
Bsp119I TTCGAA 1 cut(s) 2987
Bsp1286I GDGCHC 2 cut(s) 2815, 3001
Bsp13I TCCGGA 2 cut(s) 1329, 3178
Bsp143I GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
Bsp19I CCATGG 1 cut(s) 1401
BspACI CCGC 4 cut(s) 737, 1293, 1634, 2477
BspANI GGCC 7 cut(s) 158, 363, 940, 1266, 1270, 1579, 2305
BspCNI CTCAG 8 cut(s) 309, 825, 850, 1780, 1951, 1952, 2933, 3157
BspEI TCCGGA 2 cut(s) 1329, 3178
BspHI TCATGA 1 cut(s) 2725
BspLI GGNNCC 7 cut(s) 304, 402, 1097, 1298, 1328, 1477, 3177
BspMAI CTGCAG 1 cut(s) 3219
BspPI GGATC 2 cut(s) 215, 3238
BspQI GCTCTTC 1 cut(s) 1326
BspT104I TTCGAA 1 cut(s) 2987
BsrDI GCAATG 1 cut(s) 2918
BsrFI RCCGGY 1 cut(s) 1266
BsrI ACTGG 5 cut(s) 130, 206, 956, 2713, 3032
BssAI RCCGGY 1 cut(s) 1266
BssECI CCNNGG 8 cut(s) 975, 1091, 1184, 1401, 1540, 2568, 2780, 3170
BssMI GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
BssNAI GTATAC 1 cut(s) 624
BssNI GRCGYC 1 cut(s) 573
BssT1I CCWWGG 6 cut(s) 975, 1091, 1184, 1401, 1540, 2780
Bst1107I GTATAC 1 cut(s) 624
Bst2UI CCWGG 4 cut(s) 1253, 2250, 2570, 3171
Bst6I CTCTTC 4 cut(s) 676, 997, 1326, 1909
BstACI GRCGYC 1 cut(s) 573
BstBAI YACGTR 1 cut(s) 1274
BstBI TTCGAA 1 cut(s) 2987
BstC8I GCNNGC 7 cut(s) 487, 942, 1268, 1811, 1982, 2043, 3167
BstDSI CCRYGG 1 cut(s) 1401
BstEII GGTNACC 1 cut(s) 344
BstF5I GGATG 5 cut(s) 243, 716, 2098, 2776, 3230
BstKTI GATC 6 cut(s) 172, 223, 583, 925, 2113, 3233
BstMAI GTCTC 7 cut(s) 1079, 1344, 1513, 1832, 2451, 2630, 2957
BstMBI GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
BstMWI GCNNNNNNNGC 6 cut(s) 107, 155, 431, 503, 2782, 2798
BstNI CCWGG 4 cut(s) 1253, 2250, 2570, 3171
BstNSI RCATGY 4 cut(s) 892, 1817, 2045, 2440
BstPAI GACNNNNGTC 1 cut(s) 473
BstPI GGTNACC 1 cut(s) 344
BstSCI CCNGG 4 cut(s) 1251, 2248, 2568, 3169
BstSFI CTRYAG 4 cut(s) 216, 471, 945, 3215
BstV1I GCAGC 6 cut(s) 358, 469, 484, 2347, 3198, 3201
BstV2I GAAGAC 1 cut(s) 2754
BstX2I RGATCY 1 cut(s) 220
BstXI CCANNNNNNTGG 1 cut(s) 2903
BstYI RGATCY 1 cut(s) 220
BstZ17I GTATAC 1 cut(s) 624
BsuI GTATCC 1 cut(s) 3175
BsuRI GGCC 7 cut(s) 158, 363, 940, 1266, 1270, 1579, 2305
BtgI CCRYGG 1 cut(s) 1401
BtsCI GGATG 5 cut(s) 243, 716, 2098, 2776, 3230
BtsI GCAGTG 2 cut(s) 762, 1596
BtsIMutI CAGTG 9 cut(s) 49, 304, 762, 1596, 2317, 2423, 2908, 2936, 3039
Cac8I GCNNGC 7 cut(s) 487, 942, 1268, 1811, 1982, 2043, 3167
CaiI CAGNNNCTG 1 cut(s) 446
CciI TCATGA 1 cut(s) 2725
Cfr10I RCCGGY 1 cut(s) 1266
Cfr13I GGNCC 6 cut(s) 277, 1181, 1241, 1264, 1537, 2659
Csp6I GTAC 4 cut(s) 1379, 1499, 2242, 2557
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviQI GTAC 4 cut(s) 1379, 1499, 2242, 2557
DpnI GATC 6 cut(s) 171, 222, 582, 924, 2112, 3232
DpnII GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
DriI GACNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 3 cut(s) 156, 938, 1268
Eam1104I CTCTTC 4 cut(s) 676, 997, 1326, 1909
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 4 cut(s) 676, 997, 1326, 1909
Ecl136II GAGCTC 2 cut(s) 2813, 2999
Eco130I CCWWGG 6 cut(s) 975, 1091, 1184, 1401, 1540, 2780
Eco24I GRGCYC 2 cut(s) 2815, 3001
Eco32I GATATC 1 cut(s) 2353
Eco47I GGWCC 5 cut(s) 277, 1181, 1241, 1537, 2659
Eco53kI GAGCTC 2 cut(s) 2813, 2999
Eco57I CTGAAG 5 cut(s) 386, 615, 770, 1599, 2860
Eco72I CACGTG 1 cut(s) 1274
Eco88I CYCGRG 1 cut(s) 2865
Eco91I GGTNACC 1 cut(s) 344
EcoICRI GAGCTC 2 cut(s) 2813, 2999
EcoO65I GGTNACC 1 cut(s) 344
EcoRI GAATTC 2 cut(s) 1780, 2077
EcoRII CCWGG 4 cut(s) 1251, 2248, 2568, 3169
EcoRV GATATC 1 cut(s) 2353
EcoT14I CCWWGG 6 cut(s) 975, 1091, 1184, 1401, 1540, 2780
EcoT22I ATGCAT 1 cut(s) 2542
EcoT38I GRGCYC 2 cut(s) 2815, 3001
ErhI CCWWGG 6 cut(s) 975, 1091, 1184, 1401, 1540, 2780
Esp3I CGTCTC 1 cut(s) 1344
FaqI GGGAC 2 cut(s) 739, 1840
FauI CCCGC 1 cut(s) 1286
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 6 cut(s) 372, 483, 498, 2336, 3212, 3215
FokI GGATG 5 cut(s) 250, 723, 2105, 2763, 3237
FriOI GRGCYC 2 cut(s) 2815, 3001
FseI GGCCGGCC 1 cut(s) 1270
Fsp4HI GCNGC 6 cut(s) 372, 483, 498, 2336, 3212, 3215
FspBI CTAG 7 cut(s) 1185, 1206, 1418, 2082, 2178, 2739, 3002
GluI GCNGC 6 cut(s) 372, 483, 498, 2336, 3212, 3215
GsuI CTGGAG 4 cut(s) 253, 2271, 2402, 3015
HaeIII GGCC 7 cut(s) 158, 363, 940, 1266, 1270, 1579, 2305
HapII CCGG 8 cut(s) 398, 1099, 1267, 1279, 1330, 2662, 3179, 3222
Hin1I GRCGYC 1 cut(s) 573
HincII GTYRAC 1 cut(s) 2607
HindII GTYRAC 1 cut(s) 2607
HindIII AAGCTT 4 cut(s) 147, 487, 1807, 1894
HpaII CCGG 8 cut(s) 398, 1099, 1267, 1279, 1330, 2662, 3179, 3222
HphI GGTGA 8 cut(s) 785, 1354, 1425, 1739, 2000, 2233, 2809, 2884
Hpy99I CGWCG 1 cut(s) 575
HpyCH4IV ACGT 5 cut(s) 573, 1273, 1377, 2268, 2675
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 155, 431, 503, 2782, 2798
HpySE526I ACGT 5 cut(s) 573, 1273, 1377, 2268, 2675
Hsp92I GRCGYC 1 cut(s) 573
Kpn2I TCCGGA 2 cut(s) 1329, 3178
KroI GCCGGC 1 cut(s) 1266
KroNI GCCGGC 1 cut(s) 1268
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
LguI GCTCTTC 1 cut(s) 1326
LmnI GCTCC 8 cut(s) 308, 400, 634, 1053, 1171, 1475, 1714, 2590
Lsp1109I GCAGC 6 cut(s) 358, 469, 484, 2347, 3198, 3201
LweI GCATC 4 cut(s) 412, 917, 2674, 2785
MaeI CTAG 7 cut(s) 1185, 1206, 1418, 2082, 2178, 2739, 3002
MaeII ACGT 5 cut(s) 573, 1273, 1377, 2268, 2675
MalI GATC 6 cut(s) 171, 222, 582, 924, 2112, 3232
MboI GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
MfeI CAATTG 2 cut(s) 1652, 2560
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 2 cut(s) 2815, 3001
MlsI TGGCCA 1 cut(s) 158
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 9 cut(s) 437, 721, 1534, 1622, 2177, 2453, 2612, 2716, 2857
MmeI TCCRAC 1 cut(s) 2628
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 2542
MroI TCCGGA 2 cut(s) 1329, 3178
MroNI GCCGGC 1 cut(s) 1266
MroXI GAANNNNTTC 1 cut(s) 870
MscI TGGCCA 1 cut(s) 158
MslI CAYNNNNRTG 3 cut(s) 930, 1722, 2901
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 3134
MspI CCGG 8 cut(s) 398, 1099, 1267, 1279, 1330, 2662, 3179, 3222
MspR9I CCNGG 4 cut(s) 1253, 2250, 2570, 3171
MunI CAATTG 2 cut(s) 1652, 2560
Mva1269I GAATGC 3 cut(s) 2371, 2457, 2540
MvaI CCWGG 4 cut(s) 1253, 2250, 2570, 3171
MwoI GCNNNNNNNGC 6 cut(s) 107, 155, 431, 503, 2782, 2798
NaeI GCCGGC 1 cut(s) 1268
NcoI CCATGG 1 cut(s) 1401
NdeII GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
NgoMIV GCCGGC 1 cut(s) 1266
NlaIV GGNNCC 7 cut(s) 304, 402, 1097, 1298, 1328, 1477, 3177
NmuCI GTSAC 4 cut(s) 2180, 2465, 2890, 3034
NsiI ATGCAT 1 cut(s) 2542
NspI RCATGY 4 cut(s) 892, 1817, 2045, 2440
NspV TTCGAA 1 cut(s) 2987
PaeI GCATGC 1 cut(s) 2045
PagI TCATGA 1 cut(s) 2725
PciI ACATGT 1 cut(s) 2436
PciSI GCTCTTC 1 cut(s) 1326
PctI GAATGC 3 cut(s) 2371, 2457, 2540
PdiI GCCGGC 1 cut(s) 1268
PdmI GAANNNNTTC 1 cut(s) 870
PfeI GAWTC 8 cut(s) 826, 1145, 1202, 1406, 1943, 2156, 2231, 2420
PfoI TCCNGGA 2 cut(s) 1251, 2248
PkrI GCNGC 6 cut(s) 373, 484, 499, 2337, 3213, 3216
PleI GAGTC 9 cut(s) 437, 721, 1533, 1622, 2177, 2452, 2611, 2716, 2857
PmaCI CACGTG 1 cut(s) 1274
PmlI CACGTG 1 cut(s) 1274
PpsI GAGTC 9 cut(s) 437, 721, 1533, 1622, 2177, 2452, 2611, 2716, 2857
Ppu21I YACGTR 1 cut(s) 1274
PscI ACATGT 1 cut(s) 2436
PshAI GACNNNNGTC 1 cut(s) 473
PsiI TTATAA 1 cut(s) 512
Psp124BI GAGCTC 2 cut(s) 2815, 3001
Psp6I CCWGG 4 cut(s) 1251, 2248, 2568, 3169
PspCI CACGTG 1 cut(s) 1274
PspEI GGTNACC 1 cut(s) 344
PspGI CCWGG 4 cut(s) 1251, 2248, 2568, 3169
PspN4I GGNNCC 7 cut(s) 304, 402, 1097, 1298, 1328, 1477, 3177
PspPI GGNCC 6 cut(s) 277, 1181, 1241, 1264, 1537, 2659
PstI CTGCAG 1 cut(s) 3219
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 3134
RigI GGCCGGCC 1 cut(s) 1270
RsaI GTAC 4 cut(s) 1380, 1500, 2243, 2558
RsaNI GTAC 4 cut(s) 1379, 1499, 2242, 2557
RseI CAYNNNNRTG 3 cut(s) 930, 1722, 2901
SacI GAGCTC 2 cut(s) 2815, 3001
SapI GCTCTTC 1 cut(s) 1326
SatI GCNGC 6 cut(s) 372, 483, 498, 2336, 3212, 3215
Sau3AI GATC 6 cut(s) 169, 220, 580, 922, 2110, 3230
Sau96I GGNCC 6 cut(s) 277, 1181, 1241, 1264, 1537, 2659
SchI GAGTC 9 cut(s) 437, 721, 1534, 1622, 2177, 2453, 2612, 2716, 2857
ScrFI CCNGG 4 cut(s) 1253, 2250, 2570, 3171
SduI GDGCHC 2 cut(s) 2815, 3001
SfaNI GCATC 4 cut(s) 412, 917, 2674, 2785
SfcI CTRYAG 4 cut(s) 216, 471, 945, 3215
SfuI TTCGAA 1 cut(s) 2987
SinI GGWCC 5 cut(s) 277, 1181, 1241, 1537, 2659
SmiMI CAYNNNNRTG 3 cut(s) 930, 1722, 2901
SmlI CTYRAG 1 cut(s) 2786
SmoI CTYRAG 1 cut(s) 2786
SphI GCATGC 1 cut(s) 2045
SsiI CCGC 4 cut(s) 737, 1293, 1634, 2477
SspMI CTAG 7 cut(s) 1185, 1206, 1418, 2082, 2178, 2739, 3002
SstI GAGCTC 2 cut(s) 2815, 3001
StyD4I CCNGG 4 cut(s) 1251, 2248, 2568, 3169
StyI CCWWGG 6 cut(s) 975, 1091, 1184, 1401, 1540, 2780
TaiI ACGT 5 cut(s) 576, 1276, 1380, 2271, 2678
TaqI TCGA 4 cut(s) 570, 913, 2299, 2987
TatI WGTACW 1 cut(s) 2556
TfiI GAWTC 8 cut(s) 826, 1145, 1202, 1406, 1943, 2156, 2231, 2420
TscAI CASTG 9 cut(s) 49, 304, 769, 1603, 2317, 2430, 2908, 2936, 3039
TseFI GTSAC 4 cut(s) 2180, 2465, 2890, 3034
TseI GCWGC 6 cut(s) 371, 482, 497, 2335, 3211, 3214
Tsp45I GTSAC 4 cut(s) 2180, 2465, 2890, 3034
TspGWI ACGGA 4 cut(s) 1210, 2453, 3077, 3093
TspRI CASTG 9 cut(s) 49, 304, 769, 1603, 2317, 2430, 2908, 2936, 3039
VpaK11BI GGWCC 5 cut(s) 277, 1181, 1241, 1537, 2659
XbaI TCTAGA 3 cut(s) 1205, 2738, 3001
XceI RCATGY 4 cut(s) 892, 1817, 2045, 2440
XcmI CCANNNNNNNNNTGG 2 cut(s) 948, 1444
XmaJI CCTAGG 1 cut(s) 1184
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 870
XspI CTAG 7 cut(s) 1185, 1206, 1418, 2082, 2178, 2739, 3002
ZraI GACGTC 1 cut(s) 574
Zsp2I ATGCAT 1 cut(s) 2542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.