RchiOBHm_Chr5g0001751

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008901
Physical Location & Seq
Reverse (-)
963281 .. 964382
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28316

Sequence Viewer

Length: 366 bp
ATGGTCTCAATGCCAACGATTAAGCCCCCGCTCTCTCTTGATTTCGACACCTCTGTTTTCAACAAGGAGAAGATCAACCTCGCTGGTCACGATGAGTATATTGTAAAGGGAGAAAGGTACTTGTTCCATCTGCCGCCTGATGCCTTCAAGGGCATCAAGCAGATCGGTGTTATCGGTTGGGGTTCACAGGGACCTGCTCAAGCTCAAAACTTAAGAGATTCTCTAGCTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTTAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACCGAGGAGAATGGGACTTTAGGTGACATATATATGGGAAACAATCTCAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

121

Amino Acids

13.29

Weight (kDa)

7.99

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 53 - 104 1.7e-10 Acetohydroxy acid isomeroreductase, NADPH-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccBSI CCGCTC 1 cut(s) 31
AccII CGCG 1 cut(s) 297
AciI CCGC 2 cut(s) 29, 134
AclWI GGATC 2 cut(s) 267, 280
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 1 cut(s) 119
AflII CTTAAG 1 cut(s) 211
AgsI TTSAA 2 cut(s) 61, 148
AluBI AGCT 4 cut(s) 203, 227, 293, 302
AluI AGCT 4 cut(s) 203, 227, 293, 302
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 2 cut(s) 267, 280
ApeKI GCWGC 1 cut(s) 299
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 301, 344
AvaII GGWCC 1 cut(s) 191
BamHI GGATCC 1 cut(s) 272
BbvI GCAGC 1 cut(s) 311
BccI CCATC 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 1 cut(s) 224
BfrI CTTAAG 1 cut(s) 211
BfuAI ACCTGC 1 cut(s) 202
BisI GCNGC 2 cut(s) 134, 300
BlsI GCNGC 2 cut(s) 135, 301
Bme18I GGWCC 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 2 cut(s) 192, 274
BmsI GCATC 2 cut(s) 130, 162
Bpu10I CCTNAGC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 297
BslFI GGGAC 2 cut(s) 204, 337
BsmAI GTCTC 1 cut(s) 10
BsmFI GGGAC 2 cut(s) 204, 337
Bso31I GGTCTC 1 cut(s) 10
Bsp143I GATC 3 cut(s) 72, 162, 272
BspACI CCGC 2 cut(s) 29, 134
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 192, 274
BspMI ACCTGC 1 cut(s) 202
BspPI GGATC 2 cut(s) 267, 280
BspTI CTTAAG 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 10
BsrBI CCGCTC 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 3 cut(s) 72, 162, 272
BstAFI CTTAAG 1 cut(s) 211
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 263, 356
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 3 cut(s) 75, 165, 275
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 3 cut(s) 72, 162, 272
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 272
BstYI RGATCY 1 cut(s) 272
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 118
CviJI RGCY 6 cut(s) 25, 203, 227, 262, 293, 302
CviKI_1 RGCY 6 cut(s) 25, 203, 227, 262, 293, 302
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 2 cut(s) 263, 356
DpnI GATC 3 cut(s) 74, 164, 274
DpnII GATC 3 cut(s) 72, 162, 272
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 191
FaiI YATR 5 cut(s) 99, 338, 340, 342, 344
FaqI GGGAC 2 cut(s) 204, 337
FauI CCCGC 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 134, 300
Fsp4HI GCNGC 2 cut(s) 134, 300
FspBI CTAG 1 cut(s) 224
GlaI GCGC 1 cut(s) 298
GluI GCNGC 2 cut(s) 134, 300
HhaI GCGC 1 cut(s) 299
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 1 cut(s) 218
HphI GGTGA 2 cut(s) 301, 344
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 3 cut(s) 38, 89, 253
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 154, 262
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 2 cut(s) 263, 356
HspAI GCGC 1 cut(s) 297
Kzo9I GATC 3 cut(s) 72, 162, 272
LpnPI CCDG 5 cut(s) 69, 150, 173, 207, 288
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 2 cut(s) 130, 162
MaeI CTAG 1 cut(s) 224
MaeIII GTNAC 2 cut(s) 86, 332
MalI GATC 3 cut(s) 74, 164, 274
MbiI CCGCTC 1 cut(s) 31
MboI GATC 3 cut(s) 72, 162, 272
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 272
MnlI CCTC 3 cut(s) 61, 89, 307
MseI TTAA 3 cut(s) 21, 212, 285
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 211
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeII GATC 3 cut(s) 72, 162, 272
NlaIV GGNNCC 2 cut(s) 192, 274
NmuCI GTSAC 2 cut(s) 86, 332
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 2 cut(s) 135, 301
PpuMI RGGWCCY 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 191
PspN4I GGNNCC 2 cut(s) 192, 274
PspPI GGNCC 1 cut(s) 191
PspPPI RGGWCCY 1 cut(s) 191
PsuI RGATCY 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
RseI CAYNNNNRTG 1 cut(s) 341
SaqAI TTAA 3 cut(s) 21, 212, 285
SatI GCNGC 2 cut(s) 134, 300
Sau3AI GATC 3 cut(s) 72, 162, 272
Sau96I GGNCC 1 cut(s) 191
SetI ASST 9 cut(s) 53, 81, 119, 196, 205, 229, 295, 304, 334
SfaNI GCATC 2 cut(s) 130, 162
SinI GGWCC 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 198, 211
SmoI CTYRAG 2 cut(s) 198, 211
SsiI CCGC 2 cut(s) 29, 134
SspI AATATT 1 cut(s) 244
SspMI CTAG 1 cut(s) 224
TaqI TCGA 2 cut(s) 45, 239
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 3 cut(s) 21, 212, 285
Tru9I TTAA 3 cut(s) 21, 212, 285
TseFI GTSAC 2 cut(s) 86, 332
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 2 cut(s) 86, 332
TspDTI ATGAA 2 cut(s) 270, 303
Vha464I CTTAAG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 191
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.