Rmu_sc0002083.1_g000023

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002083.1
OG0008901
Physical Location & Seq
Forward (+)
105975 .. 113091
7117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002083.1_g000023.1.cds

Sequence Viewer

Length: 1278 bp
atgcgactgcttaatgatttgctccaatggattgtgcggcccgaggatcgaaggtctatgaccttgggcagaatatcaggtaatcacgtctttggccggacgagtggttacgtttcaggatggctccaccttcatcctctaaagcccacacggtggactctcgccttctcctccgccgtctccaagtccctcaaccctctcaggctcagagccgcctctcgcggtgctgctattggccgcggatcctctctcagagctcgtatggtctcaatgctgacgattaagcccccgctctctcttgatttcgacacctctgttttcaacaaggagaagatcaacctcgctggtcacgatgagtatattgtaaagggagaaaggtacttgttccatctgccgcctgatgccttcccaggtgtatataaagatttggatgaggtgttgcagtcaaagacggtgtacttgaatgtttccaaaggtgttcttgccaagaccaaggatttggtagctgcgtttgggtcggatgatcagaccaagatttgtttagagatcttggataaaggggagcttcaggttgctgggaaggagagagagtctcagttgtccagtcaatttcgggatatcgccactattgttatgcagaaaacctataatccagagaaggcatcaagcagatcggtgttatcgggacctgctcaagctcagaacttaagagattctctagctgaaacaaagtcgaatattgtggtcaagattgggctcaggaagggatcccgttcatttaatgaagctcgcacagctggtttcaccgaggagaatgggactttaggtgacatatgggaaacaatctcagagagtgatcttgtgttgctactaatttctgatcccgcacagggcatgttggaaatatacagctctttgtctgaagctgacaagaaggagtttgagactgcatatagtgcctcctattatccttgcatggacatcctctatgaatgctataaggatgtagcaagcggcagttatttcgttcctgacattgtggtggctgcagtaattggagttatgaccagttggcctgtaggccctctggtacctatttgtggtcgttggttagccaggtcatccattttgcaagtcttattgcatcctagtgtgctagacttggctctgtcatctcagttctttccatatagcacggctgcacctaagagagttgtcttccagcatacttttcttactgcaggtatccaaactagcacgagttatcatgtgaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

425

Amino Acids

47.0

Weight (kDa)

8.82

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 697, 1232
Acc65I GGTACC 1 cut(s) 1090
AccB1I GGYRCC 1 cut(s) 1090
AccB7I CCANNNNNTGG 1 cut(s) 153
AccBSI CCGCTC 1 cut(s) 292
AccII CGCG 2 cut(s) 222, 240
AclWI GGATC 6 cut(s) 54, 237, 250, 762, 775, 875
AcoI YGGCCR 2 cut(s) 94, 235
AcuI CTGAAG 2 cut(s) 551, 942
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 3 cut(s) 380, 458, 1092
AfiI CCNNNNNNNGG 3 cut(s) 153, 890, 1100
AflII CTTAAG 1 cut(s) 706
AgsI TTSAA 2 cut(s) 322, 463
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 2 cut(s) 409, 1115
AluBI AGCT 9 cut(s) 257, 506, 565, 698, 722, 788, 797, 912, 926
AluI AGCT 9 cut(s) 257, 506, 565, 698, 722, 788, 797, 912, 926
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 4 cut(s) 184, 271, 597, 938
AlwI GGATC 6 cut(s) 54, 237, 250, 762, 775, 875
Ama87I CYCGRG 1 cut(s) 41
AoxI GGCC 5 cut(s) 38, 94, 235, 1073, 1081
ApeKI GCWGC 4 cut(s) 227, 506, 1046, 1199
ArsI GACNNNNNNTTYG 2 cut(s) 481, 513
Asp718I GGTACC 1 cut(s) 1090
AspS9I GGNCC 3 cut(s) 39, 686, 1082
AsuHPI GGTGA 2 cut(s) 796, 839
AvaI CYCGRG 1 cut(s) 41
AvaII GGWCC 1 cut(s) 686
BamHI GGATCC 2 cut(s) 242, 767
BanI GGYRCC 1 cut(s) 1090
BanII GRGCYC 2 cut(s) 259, 759
BauI CACGAG 1 cut(s) 1258
BbsI GAAGAC 1 cut(s) 1210
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 4 cut(s) 214, 493, 1033, 1186
BccI CCATC 2 cut(s) 114, 396
BceAI ACGGC 2 cut(s) 161, 1212
BcgI CGANNNNNNTGC 2 cut(s) 1006, 1040
BciT130I CCWGG 2 cut(s) 411, 1117
BciVI GTATCC 1 cut(s) 1256
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 4 cut(s) 184, 271, 597, 938
BfaI CTAG 4 cut(s) 719, 1149, 1157, 1254
BfmI CTRYAG 3 cut(s) 1047, 1077, 1239
BfrI CTTAAG 1 cut(s) 706
BfuAI ACCTGC 2 cut(s) 697, 1232
BfuI GTATCC 1 cut(s) 1256
BglII AGATCT 1 cut(s) 546
BisI GCNGC 9 cut(s) 38, 213, 228, 238, 395, 507, 1015, 1047, 1200
BlsI GCNGC 9 cut(s) 39, 214, 229, 239, 396, 508, 1016, 1048, 1201
Bme1390I CCNGG 2 cut(s) 411, 1117
Bme18I GGWCC 1 cut(s) 686
BmeT110I CYCGRG 1 cut(s) 41
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 3 cut(s) 39, 686, 1082
BmiI GGNNCC 5 cut(s) 125, 244, 687, 769, 1092
BmrFI CCNGG 2 cut(s) 411, 1117
BmsI GCATC 3 cut(s) 391, 671, 1153
BpiI GAAGAC 1 cut(s) 1210
BplI GAGNNNNNCTC 2 cut(s) 577, 609
Bpu10I CCTNAGC 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 678
BsaI GGTCTC 1 cut(s) 271
BsaJI CCNNGG 6 cut(s) 42, 63, 238, 409, 492, 807
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 890, 1100
Bse1I ACTGG 2 cut(s) 603, 1068
BseBI CCWGG 2 cut(s) 411, 1117
BseDI CCNNGG 6 cut(s) 42, 63, 238, 409, 492, 807
BseGI GGATG 8 cut(s) 125, 133, 436, 526, 981, 1009, 1121, 1144
BseLI CCNNNNNNNGG 3 cut(s) 153, 890, 1100
BseMII CTCAG 8 cut(s) 214, 220, 265, 608, 713, 772, 861, 1190
BseNI ACTGG 2 cut(s) 603, 1068
BseRI GAGGAG 2 cut(s) 160, 824
BseXI GCAGC 4 cut(s) 214, 493, 1033, 1186
BseYI CCCAGC 1 cut(s) 575
BsgI GTGCAG 1 cut(s) 1185
Bsh1236I CGCG 2 cut(s) 222, 240
BshFI GGCC 5 cut(s) 40, 96, 237, 1075, 1083
BshNI GGYRCC 1 cut(s) 1090
BsiHKAI GWGCWC 1 cut(s) 259
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 1 cut(s) 97
BslFI GGGAC 3 cut(s) 172, 699, 832
BslI CCNNNNNNNGG 3 cut(s) 153, 890, 1100
BsmAI GTCTC 4 cut(s) 184, 271, 597, 938
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 3 cut(s) 172, 699, 832
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 5 cut(s) 40, 96, 237, 1075, 1083
Bso31I GGTCTC 1 cut(s) 271
BsoBI CYCGRG 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 259, 759
Bsp143I GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
BspANI GGCC 5 cut(s) 40, 96, 237, 1075, 1083
BspCNI CTCAG 8 cut(s) 213, 219, 264, 607, 712, 771, 860, 1189
BspFNI CGCG 2 cut(s) 222, 240
BspLI GGNNCC 5 cut(s) 125, 244, 687, 769, 1092
BspMAI CTGCAG 2 cut(s) 1051, 1243
BspMI ACCTGC 2 cut(s) 697, 1232
BspPI GGATC 6 cut(s) 54, 237, 250, 762, 775, 875
BspT107I GGYRCC 1 cut(s) 1090
BspTI CTTAAG 1 cut(s) 706
BspTNI GGTCTC 1 cut(s) 271
BsrBI CCGCTC 1 cut(s) 292
BsrI ACTGG 2 cut(s) 603, 1068
BssECI CCNNGG 6 cut(s) 42, 63, 238, 409, 492, 807
BssMI GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
BssSI CACGAG 1 cut(s) 1258
BssT1I CCWWGG 2 cut(s) 63, 492
Bst2BI CACGAG 1 cut(s) 1258
Bst2UI CCWGG 2 cut(s) 411, 1117
Bst4CI ACNGT 2 cut(s) 153, 454
BstAFI CTTAAG 1 cut(s) 706
BstAPI GCANNNNNTGC 1 cut(s) 956
BstC8I GCNNGC 2 cut(s) 790, 1012
BstDEI CTNAG 9 cut(s) 200, 206, 251, 594, 699, 758, 847, 1176, 1206
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 8 cut(s) 125, 133, 436, 526, 981, 1009, 1121, 1144
BstFNI CGCG 2 cut(s) 222, 240
BstKTI GATC 9 cut(s) 49, 245, 336, 526, 549, 674, 770, 859, 883
BstMAI GTCTC 4 cut(s) 184, 271, 597, 938
BstMBI GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
BstMWI GCNNNNNNNGC 2 cut(s) 794, 956
BstNI CCWGG 2 cut(s) 411, 1117
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 2 cut(s) 409, 1115
BstSFI CTRYAG 3 cut(s) 1047, 1077, 1239
BstUI CGCG 2 cut(s) 222, 240
BstV1I GCAGC 4 cut(s) 214, 493, 1033, 1186
BstV2I GAAGAC 1 cut(s) 1210
BstX2I RGATCY 3 cut(s) 242, 546, 767
BstXI CCANNNNNNTGG 1 cut(s) 499
BstYI RGATCY 3 cut(s) 242, 546, 767
BsuI GTATCC 1 cut(s) 1256
BsuRI GGCC 5 cut(s) 40, 96, 237, 1075, 1083
BtgI CCRYGG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 8 cut(s) 125, 133, 436, 526, 981, 1009, 1121, 1144
BveI ACCTGC 2 cut(s) 697, 1232
Cac8I GCNNGC 2 cut(s) 790, 1012
Cfr13I GGNCC 3 cut(s) 39, 686, 1082
Cfr42I CCGCGG 1 cut(s) 241
Csp6I GTAC 3 cut(s) 379, 457, 1091
CviAII CATG 3 cut(s) 895, 976, 1268
CviQI GTAC 3 cut(s) 379, 457, 1091
DdeI CTNAG 9 cut(s) 200, 206, 251, 594, 699, 758, 847, 1176, 1206
DpnI GATC 9 cut(s) 48, 244, 335, 525, 548, 673, 769, 858, 882
DpnII GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
DraIII CACNNNGTG 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 94, 235
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 257
Eco130I CCWWGG 2 cut(s) 63, 492
Eco24I GRGCYC 2 cut(s) 259, 759
Eco31I GGTCTC 1 cut(s) 271
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 686
Eco53kI GAGCTC 1 cut(s) 257
Eco57I CTGAAG 2 cut(s) 551, 942
Eco88I CYCGRG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 257
EcoO109I RGGNCCY 2 cut(s) 686, 1082
EcoRII CCWGG 2 cut(s) 409, 1115
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 2 cut(s) 63, 492
EcoT38I GRGCYC 2 cut(s) 259, 759
ErhI CCWWGG 2 cut(s) 63, 492
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 3 cut(s) 898, 979, 1271
FalI AAGNNNNNCTT 4 cut(s) 465, 497, 549, 581
FaqI GGGAC 3 cut(s) 172, 699, 832
FatI CATG 3 cut(s) 894, 975, 1267
FauI CCCGC 2 cut(s) 297, 892
FauNDI CATATG 1 cut(s) 833
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 9 cut(s) 38, 213, 228, 238, 395, 507, 1015, 1047, 1200
FokI GGATG 8 cut(s) 120, 132, 443, 533, 968, 1016, 1108, 1131
FriOI GRGCYC 2 cut(s) 259, 759
Fsp4HI GCNGC 9 cut(s) 38, 213, 228, 238, 395, 507, 1015, 1047, 1200
FspBI CTAG 4 cut(s) 719, 1149, 1157, 1254
GluI GCNGC 9 cut(s) 38, 213, 228, 238, 395, 507, 1015, 1047, 1200
GsaI CCCAGC 1 cut(s) 579
HaeIII GGCC 5 cut(s) 40, 96, 237, 1075, 1083
HapII CCGG 1 cut(s) 97
Hin1II CATG 3 cut(s) 898, 979, 1271
HinfI GANTC 3 cut(s) 157, 590, 713
HpaII CCGG 1 cut(s) 97
HphI GGTGA 2 cut(s) 796, 839
Hpy166II GTNNAC 2 cut(s) 156, 457
Hpy188I TCNGA 8 cut(s) 209, 254, 520, 528, 702, 850, 880, 922
Hpy188III TCNNGA 9 cut(s) 117, 299, 350, 614, 653, 684, 748, 760, 1031
Hpy8I GTNNAC 2 cut(s) 156, 457
HpyAV CCTTC 8 cut(s) 45, 140, 175, 415, 574, 652, 757, 928
HpyCH4III ACNGT 2 cut(s) 153, 454
HpyCH4IV ACGT 2 cut(s) 87, 111
HpyCH4V TGCA 9 cut(s) 442, 637, 950, 975, 1049, 1132, 1144, 1202, 1241
HpyF10VI GCNNNNNNNGC 2 cut(s) 794, 956
HpyF3I CTNAG 9 cut(s) 200, 206, 251, 594, 699, 758, 847, 1176, 1206
HpySE526I ACGT 2 cut(s) 87, 111
Hsp92II CATG 3 cut(s) 898, 979, 1271
KpnI GGTACC 1 cut(s) 1094
Ksp22I TGATCA 1 cut(s) 523
KspI CCGCGG 1 cut(s) 241
Kzo9I GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
LmnI GCTCC 3 cut(s) 27, 129, 562
Lsp1109I GCAGC 4 cut(s) 214, 493, 1033, 1186
LweI GCATC 3 cut(s) 391, 671, 1153
MaeI CTAG 4 cut(s) 719, 1149, 1157, 1254
MaeII ACGT 2 cut(s) 87, 111
MaeIII GTNAC 3 cut(s) 107, 347, 827
MalI GATC 9 cut(s) 48, 244, 335, 525, 548, 673, 769, 858, 882
MbiI CCGCTC 1 cut(s) 292
MboI GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
MboII GAAGA 2 cut(s) 343, 1210
MflI RGATCY 3 cut(s) 242, 546, 767
MhlI GDGCHC 2 cut(s) 259, 759
MluCI AATT 3 cut(s) 608, 873, 1053
MlyI GAGTC 2 cut(s) 151, 599
MmeI TCCRAC 2 cut(s) 498, 879
MseI TTAA 4 cut(s) 12, 282, 707, 780
MslI CAYNNNNRTG 2 cut(s) 1040, 1149
MspA1I CMGCKG 2 cut(s) 240, 797
MspCI CTTAAG 1 cut(s) 706
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 2 cut(s) 411, 1117
Mva1269I GAATGC 1 cut(s) 998
MvaI CCWGG 2 cut(s) 411, 1117
MvnI CGCG 2 cut(s) 222, 240
MwoI GCNNNNNNNGC 2 cut(s) 794, 956
NdeI CATATG 1 cut(s) 833
NdeII GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
NlaIII CATG 3 cut(s) 898, 979, 1271
NlaIV GGNNCC 5 cut(s) 125, 244, 687, 769, 1092
NmuCI GTSAC 2 cut(s) 347, 827
NspI RCATGY 1 cut(s) 898
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PctI GAATGC 1 cut(s) 998
PfeI GAWTC 1 cut(s) 713
PflMI CCANNNNNTGG 1 cut(s) 153
PkrI GCNGC 9 cut(s) 39, 214, 229, 239, 396, 508, 1016, 1048, 1201
PleI GAGTC 2 cut(s) 151, 598
PpsI GAGTC 2 cut(s) 151, 598
PpuMI RGGWCCY 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 686
Psp6I CCWGG 2 cut(s) 409, 1115
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 2 cut(s) 409, 1115
PspN4I GGNNCC 5 cut(s) 125, 244, 687, 769, 1092
PspPI GGNCC 3 cut(s) 39, 686, 1082
PspPPI RGGWCCY 1 cut(s) 686
PstI CTGCAG 2 cut(s) 1051, 1243
PsuI RGATCY 3 cut(s) 242, 546, 767
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 3 cut(s) 380, 458, 1092
RsaNI GTAC 3 cut(s) 379, 457, 1091
RseI CAYNNNNRTG 2 cut(s) 1040, 1149
SacI GAGCTC 1 cut(s) 259
SacII CCGCGG 1 cut(s) 241
SaqAI TTAA 4 cut(s) 12, 282, 707, 780
SatI GCNGC 9 cut(s) 38, 213, 228, 238, 395, 507, 1015, 1047, 1200
Sau3AI GATC 9 cut(s) 46, 242, 333, 523, 546, 671, 767, 856, 880
Sau96I GGNCC 3 cut(s) 39, 686, 1082
SchI GAGTC 2 cut(s) 151, 599
ScrFI CCNGG 2 cut(s) 411, 1117
SduI GDGCHC 2 cut(s) 259, 759
SfaNI GCATC 3 cut(s) 391, 671, 1153
SfcI CTRYAG 3 cut(s) 1047, 1077, 1239
Sfr303I CCGCGG 1 cut(s) 241
SgrBI CCGCGG 1 cut(s) 241
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 2 cut(s) 1040, 1149
SmlI CTYRAG 2 cut(s) 693, 706
SmoI CTYRAG 2 cut(s) 693, 706
Sse9I AATT 3 cut(s) 608, 873, 1053
SspI AATATT 1 cut(s) 739
SspMI CTAG 4 cut(s) 719, 1149, 1157, 1254
SstI GAGCTC 1 cut(s) 259
StyD4I CCNGG 2 cut(s) 409, 1115
StyI CCWWGG 2 cut(s) 63, 492
TaaI ACNGT 2 cut(s) 153, 454
TaiI ACGT 2 cut(s) 90, 114
TaqI TCGA 3 cut(s) 49, 306, 734
TasI AATT 3 cut(s) 608, 873, 1053
TatI WGTACW 1 cut(s) 456
TauI GCSGC 5 cut(s) 40, 215, 240, 397, 1017
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 4 cut(s) 12, 282, 707, 780
Tru9I TTAA 4 cut(s) 12, 282, 707, 780
TseFI GTSAC 2 cut(s) 347, 827
TseI GCWGC 4 cut(s) 227, 506, 1046, 1199
Tsp45I GTSAC 2 cut(s) 347, 827
TspDTI ATGAA 4 cut(s) 122, 765, 798, 1005
Van91I CCANNNNNTGG 1 cut(s) 153
Vha464I CTTAAG 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 686
XceI RCATGY 1 cut(s) 898
XspI CTAG 4 cut(s) 719, 1149, 1157, 1254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.