Rh5BG548400

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008901
Physical Location & Seq
Forward (+)
87107576 .. 87108958
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG548400.1

Sequence Viewer

Length: 696 bp
ATGCCTTTCCGGTTCAGAATTTTCTCCAAACAGTGCCATGTGGATTTCGTCATCACCATTTCTATCAACCTGGATGAGCAGCTGATGGGTTCGTCAGGGATGGCTCCACCTTCATCCTCTAAAGCCCACGCGGCGGCTCCCTCCACTCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCAGAGCCGCCTCTCGCGGTGCTGCTATTGGCCGCGGATCCGCTCTCAGAGCTCGTATGGTCTCAATGCCGACGATTAAGCCCCCGCTCTCTCGTGATTTCGACACCTCTGTTTTCAACAAGGAGAATATCAACCTCGCTGGTCACGATGAGAGTATTCATAGTGATTTGAGATATGGTATTTGGTTTTTGTTGATTTTGCAGTATATTGTAAAGGGAGAAAGGTACTTGTTCCATCTGCCGCCTGATGCCTTCAACTCTTCAAGGACATCAAGCATATCGGTGTTATCGGTTGGGGGACCTGCTCAAGCTCAGAACTTAAGAGATTCTCTAGCTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCATCGAGGAGAATGGGACTTTAGGTGACATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

231

Amino Acids

25.1

Weight (kDa)

9.37

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 502
AccBSI CCGCTC 2 cut(s) 236, 280
AccII CGCG 4 cut(s) 131, 210, 228, 597
AclWI GGATC 5 cut(s) 225, 238, 567, 580, 680
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 133
AflII CTTAAG 1 cut(s) 511
AgsI TTSAA 3 cut(s) 310, 448, 456
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 6 cut(s) 82, 245, 503, 527, 593, 602
AluI AGCT 6 cut(s) 82, 245, 503, 527, 593, 602
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 2 cut(s) 172, 259
AlwI GGATC 5 cut(s) 225, 238, 567, 580, 680
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 3 cut(s) 79, 215, 599
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 1 cut(s) 599
AspS9I GGNCC 1 cut(s) 491
AsuHPI GGTGA 2 cut(s) 46, 644
AvaII GGWCC 1 cut(s) 491
BamHI GGATCC 2 cut(s) 230, 572
BanII GRGCYC 2 cut(s) 247, 564
BauI CACGAG 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 91, 202, 611
BccI CCATC 3 cut(s) 79, 94, 435
BceAI ACGGC 1 cut(s) 149
BciT130I CCWGG 1 cut(s) 71
BcoDI GTCTC 2 cut(s) 172, 259
BfaI CTAG 1 cut(s) 524
BfrI CTTAAG 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 502
BglI GCCNNNNNGGC 1 cut(s) 131
BisI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 434, 600
BlsI GCNGC 8 cut(s) 81, 133, 136, 202, 217, 227, 435, 601
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 5 cut(s) 105, 138, 232, 492, 574
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 3 cut(s) 79, 105, 113
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 6 cut(s) 202, 208, 253, 518, 577, 666
BseRI GAGGAG 2 cut(s) 148, 629
BseXI GCAGC 3 cut(s) 91, 202, 611
Bsh1236I CGCG 4 cut(s) 131, 210, 228, 597
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 3 cut(s) 160, 504, 637
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 2 cut(s) 172, 259
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 3 cut(s) 160, 504, 637
BsnI GGCC 1 cut(s) 225
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 247, 564
Bsp143I GATC 4 cut(s) 230, 572, 661, 685
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 6 cut(s) 201, 207, 252, 517, 576, 665
BspFNI CGCG 4 cut(s) 131, 210, 228, 597
BspLI GGNNCC 5 cut(s) 105, 138, 232, 492, 574
BspMI ACCTGC 1 cut(s) 502
BspPI GGATC 5 cut(s) 225, 238, 567, 580, 680
BspTI CTTAAG 1 cut(s) 511
BspTNI GGTCTC 1 cut(s) 259
BsrBI CCGCTC 2 cut(s) 236, 280
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 4 cut(s) 230, 572, 661, 685
BssSI CACGAG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 285
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 33
Bst6I CTCTTC 1 cut(s) 457
BstAFI CTTAAG 1 cut(s) 511
BstC8I GCNNGC 1 cut(s) 595
BstDEI CTNAG 6 cut(s) 188, 194, 239, 504, 563, 652
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 3 cut(s) 79, 105, 113
BstFNI CGCG 4 cut(s) 131, 210, 228, 597
BstHHI GCGC 1 cut(s) 599
BstKTI GATC 4 cut(s) 233, 575, 664, 688
BstMAI GTCTC 2 cut(s) 172, 259
BstMBI GATC 4 cut(s) 230, 572, 661, 685
BstMWI GCNNNNNNNGC 3 cut(s) 131, 242, 599
BstNI CCWGG 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 69
BstUI CGCG 4 cut(s) 131, 210, 228, 597
BstV1I GCAGC 3 cut(s) 91, 202, 611
BstX2I RGATCY 2 cut(s) 230, 572
BstYI RGATCY 2 cut(s) 230, 572
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 3 cut(s) 79, 105, 113
BtsIMutI CAGTG 1 cut(s) 38
BveI ACCTGC 1 cut(s) 502
Cac8I GCNNGC 1 cut(s) 595
CfoI GCGC 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 491
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 1 cut(s) 418
CviAII CATG 1 cut(s) 38
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 6 cut(s) 188, 194, 239, 504, 563, 652
DpnI GATC 4 cut(s) 232, 574, 663, 687
DpnII GATC 4 cut(s) 230, 572, 661, 685
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 457
EciI GGCGGA 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 2 cut(s) 247, 564
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 549
EcoICRI GAGCTC 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 69
EcoT38I GRGCYC 2 cut(s) 247, 564
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 1 cut(s) 41
FaiI YATR 9 cut(s) 39, 251, 354, 369, 399, 470, 638, 640, 694
FaqI GGGAC 3 cut(s) 160, 504, 637
FatI CATG 1 cut(s) 37
FauI CCCGC 1 cut(s) 285
FauNDI CATATG 1 cut(s) 638
Fnu4HI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 434, 600
FokI GGATG 3 cut(s) 86, 100, 112
FriOI GRGCYC 2 cut(s) 247, 564
Fsp4HI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 434, 600
FspBI CTAG 1 cut(s) 524
GlaI GCGC 1 cut(s) 598
GluI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 434, 600
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 10
HhaI GCGC 1 cut(s) 599
Hin1II CATG 1 cut(s) 41
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HinfI GANTC 1 cut(s) 518
HpaII CCGG 1 cut(s) 10
HphI GGTGA 2 cut(s) 46, 644
Hpy188I TCNGA 6 cut(s) 17, 197, 242, 507, 655, 685
Hpy188III TCNNGA 4 cut(s) 287, 338, 553, 565
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 4 cut(s) 120, 163, 454, 562
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 394
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 242, 599
HpyF3I CTNAG 6 cut(s) 188, 194, 239, 504, 563, 652
Hsp92II CATG 1 cut(s) 41
HspAI GCGC 1 cut(s) 597
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 4 cut(s) 230, 572, 661, 685
LmnI GCTCC 2 cut(s) 109, 142
Lsp1109I GCAGC 3 cut(s) 91, 202, 611
LweI GCATC 1 cut(s) 430
MaeI CTAG 1 cut(s) 524
MaeIII GTNAC 2 cut(s) 335, 632
MalI GATC 4 cut(s) 232, 574, 663, 687
MbiI CCGCTC 2 cut(s) 236, 280
MboI GATC 4 cut(s) 230, 572, 661, 685
MboII GAAGA 1 cut(s) 444
MflI RGATCY 2 cut(s) 230, 572
MhlI GDGCHC 2 cut(s) 247, 564
MluCI AATT 2 cut(s) 18, 678
MnlI CCTC 9 cut(s) 127, 151, 169, 188, 195, 214, 310, 338, 607
MseI TTAA 3 cut(s) 270, 512, 585
MslI CAYNNNNRTG 1 cut(s) 473
MspA1I CMGCKG 3 cut(s) 82, 228, 602
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 71
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 4 cut(s) 131, 210, 228, 597
MwoI GCNNNNNNNGC 3 cut(s) 131, 242, 599
NdeI CATATG 1 cut(s) 638
NdeII GATC 4 cut(s) 230, 572, 661, 685
NlaIII CATG 1 cut(s) 41
NlaIV GGNNCC 5 cut(s) 105, 138, 232, 492, 574
NmuCI GTSAC 2 cut(s) 335, 632
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PfeI GAWTC 1 cut(s) 518
PkrI GCNGC 8 cut(s) 81, 133, 136, 202, 217, 227, 435, 601
PpuMI RGGWCCY 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 5 cut(s) 105, 138, 232, 492, 574
PspPI GGNCC 1 cut(s) 491
PspPPI RGGWCCY 1 cut(s) 491
PsuI RGATCY 2 cut(s) 230, 572
PvuII CAGCTG 2 cut(s) 82, 602
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 1 cut(s) 473
SacI GAGCTC 1 cut(s) 247
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 3 cut(s) 270, 512, 585
SatI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 434, 600
Sau3AI GATC 4 cut(s) 230, 572, 661, 685
Sau96I GGNCC 1 cut(s) 491
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 2 cut(s) 247, 564
SfaNI GCATC 1 cut(s) 430
Sfr303I CCGCGG 1 cut(s) 229
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 473
SmlI CTYRAG 2 cut(s) 498, 511
SmoI CTYRAG 2 cut(s) 498, 511
Sse9I AATT 2 cut(s) 18, 678
SspI AATATT 1 cut(s) 544
SspMI CTAG 1 cut(s) 524
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 294, 539, 612
TasI AATT 2 cut(s) 18, 678
TauI GCSGC 5 cut(s) 134, 137, 203, 228, 436
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 3 cut(s) 270, 512, 585
Tru9I TTAA 3 cut(s) 270, 512, 585
TscAI CASTG 1 cut(s) 38
TseFI GTSAC 2 cut(s) 335, 632
TseI GCWGC 3 cut(s) 79, 215, 599
Tsp45I GTSAC 2 cut(s) 335, 632
TspDTI ATGAA 5 cut(s) 102, 341, 570, 598, 603
TspRI CASTG 1 cut(s) 38
Vha464I CTTAAG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 491
XapI RAATTY 1 cut(s) 18
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.