Rh2AG337200

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008901
Physical Location & Seq
Forward (+)
48896269 .. 48901087
4819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG337200.1

Sequence Viewer

Length: 1287 bp
ATGTCGGCTGCAACTTCCTCCTTCTCTCCAGCCCTCACCGCTACTCCACCTTCATCCTCCAAAGCCCACGCGGCGGCTCCCTCCACTCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCAGAGCCGCCTCTCGCGCTGCTGCTGTTGGCCGCGGATCCGCTCTCGGTGCTCGTATGGTCTCAATGCCGACGATTAAGGCCCCGCTCTCTCTTGATTTCGACACCTCTGTTTTCAACAAGGAGAAGGTCAACCTCGCTGGTCACGATGAGTATATTGTAAAGGGAGGAAGGGACTTGTTCCATCTGCTGCCCGATGCCTTCAAGGGCATCAAGCAGATCGGTGTTATCGGTTGGGGTTCCCAGGGACCTGCTCAAGCTCAGAACTTGAGAGATTCTCTAGCTGAAGCAAAGTCAAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACTGAGGAGAATGGGACTTTAGGCGATATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATTCTGCACAGGCAGATAATTATGAGAAAATATTTTCACACATGAAACCAAATAGCATACTTGGTCTTTCCCACGGTTTCCTTCTAGGGCATTTGCAGTCGGAGGGACTTGACTTTCCCAAGAACATCAGTGTAATTGCTGTATGCCCAAAGGGAATGGGTCCGTCAGTAAGGAGACTTTATGTCCAAGGCAAAGAAATAAATGGTGCGGGAATTAATTCAAGTTTTGCAGTCCACCAGGATGTTGATGGGAGAGCCACAGATGTGGCTCTGGGCTGGTCGGTTGCCCTTGGTTCTCCTTTCACATTTGTCACTACGCTAGAGCAGGAATACAAAAGTGACATCTTTGGCGAGCGAGGCATACTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTCAGAAGATACACTGACAGCGGAATGAGTGAAGATTCTGCTTATAAAAATACTGTTGAATGTGTTACTGGAATTGTGTCAAGAACCATATCAACTAAGGGCATGTTGGAAATATACAGCTCTTTGTCTGAAGCTGACAAGAAGGAGTTTGAGACTGCATATAGTGCCTCCTATTATCCTTGCATGGACATCCTCTATGAATGCTATGAGGATGTAGCAAGCGGCAGTGAGATCCGCAGTGTTGTTTTGGCTGGGCGGCGATTTTATGTCTGTTCTCCTATCTGTTGCCAGTATTTAATAGTTTTTAGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

428

Amino Acids

46.01

Weight (kDa)

6.47

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 116 - 290 8.6e-32 Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C PF01450 299 - 408 5.7e-29 Ketol-acid reductoisomerase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 391
AccBSI CCGCTC 2 cut(s) 176, 220
AccII CGCG 4 cut(s) 71, 150, 168, 486
AclWI GGATC 5 cut(s) 165, 178, 456, 469, 1198
AcoI YGGCCR 1 cut(s) 163
AcuI CTGAAG 2 cut(s) 438, 1122
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 4 cut(s) 250, 337, 795, 1031
AhdI GACNNNNNGTC 1 cut(s) 755
AjnI CCWGG 2 cut(s) 375, 810
AleI CACNNNNGTG 1 cut(s) 836
AloI GAACNNNNNNTCC 2 cut(s) 956, 988
AluBI AGCT 6 cut(s) 392, 416, 482, 491, 1092, 1106
AluI AGCT 6 cut(s) 392, 416, 482, 491, 1092, 1106
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 4 cut(s) 112, 199, 742, 1118
AlwI GGATC 5 cut(s) 165, 178, 456, 469, 1198
AoxI GGCC 2 cut(s) 163, 213
ApeKI GCWGC 5 cut(s) 8, 152, 155, 322, 488
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 1 cut(s) 790
AspLEI GCGC 2 cut(s) 152, 488
AspS9I GGNCC 3 cut(s) 214, 380, 734
AsuHPI GGTGA 1 cut(s) 28
AvaII GGWCC 2 cut(s) 380, 734
BamHI GGATCC 2 cut(s) 170, 461
BanII GRGCYC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 187
BbvI GCAGC 4 cut(s) 139, 142, 309, 500
BccI CCATC 2 cut(s) 324, 815
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 2 cut(s) 377, 812
BcoDI GTCTC 4 cut(s) 112, 199, 742, 1118
BfaI CTAG 3 cut(s) 413, 659, 893
BfuAI ACCTGC 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 71
Bme1390I CCNGG 2 cut(s) 377, 812
Bme18I GGWCC 2 cut(s) 380, 734
BmeRI GACNNNNNGTC 1 cut(s) 755
BmgT120I GGNCC 3 cut(s) 214, 380, 734
BmiI GGNNCC 7 cut(s) 78, 172, 216, 373, 381, 463, 735
BmrFI CCNGG 2 cut(s) 377, 812
BmsI GCATC 2 cut(s) 319, 351
BplI GAGNNNNNCTC 2 cut(s) 394, 426
BpmI CTGGAG 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 372, 421
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 6 cut(s) 166, 375, 376, 646, 760, 862
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 956, 986
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 1045, 1261
BseBI CCWGG 2 cut(s) 377, 812
BseDI CCNNGG 6 cut(s) 166, 375, 376, 646, 760, 862
BseGI GGATG 4 cut(s) 53, 820, 1161, 1189
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 6 cut(s) 142, 148, 407, 466, 492, 555
BseNI ACTGG 2 cut(s) 1045, 1261
BseRI GAGGAG 2 cut(s) 88, 518
BseXI GCAGC 4 cut(s) 139, 142, 309, 500
BseYI CCCAGC 1 cut(s) 1223
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 4 cut(s) 71, 150, 168, 486
BshFI GGCC 2 cut(s) 165, 215
BsiHKAI GWGCWC 1 cut(s) 187
BslFI GGGAC 6 cut(s) 100, 320, 393, 526, 693, 952
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 4 cut(s) 112, 199, 742, 1118
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 6 cut(s) 100, 320, 393, 526, 693, 952
BsmI GAATGC 1 cut(s) 1178
BsnI GGCC 2 cut(s) 165, 215
Bso31I GGTCTC 1 cut(s) 199
Bsp1286I GDGCHC 2 cut(s) 187, 453
Bsp143I GATC 5 cut(s) 170, 351, 461, 550, 1203
BspANI GGCC 2 cut(s) 165, 215
BspCNI CTCAG 6 cut(s) 141, 147, 406, 465, 493, 554
BspFNI CGCG 4 cut(s) 71, 150, 168, 486
BspLI GGNNCC 7 cut(s) 78, 172, 216, 373, 381, 463, 735
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 5 cut(s) 165, 178, 456, 469, 1198
BspTNI GGTCTC 1 cut(s) 199
BsrBI CCGCTC 2 cut(s) 176, 220
BsrI ACTGG 2 cut(s) 1045, 1261
BssECI CCNNGG 6 cut(s) 166, 375, 376, 646, 760, 862
BssMI GATC 5 cut(s) 170, 351, 461, 550, 1203
BssT1I CCWWGG 2 cut(s) 760, 862
Bst2UI CCWGG 2 cut(s) 377, 812
Bst4CI ACNGT 2 cut(s) 650, 1027
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstC8I GCNNGC 3 cut(s) 484, 926, 1192
BstDEI CTNAG 7 cut(s) 128, 134, 393, 452, 501, 541, 1068
BstDSI CCRYGG 2 cut(s) 166, 646
BstF5I GGATG 4 cut(s) 53, 820, 1161, 1189
BstFNI CGCG 4 cut(s) 71, 150, 168, 486
BstHHI GCGC 2 cut(s) 152, 488
BstKTI GATC 5 cut(s) 173, 354, 464, 553, 1206
BstMAI GTCTC 4 cut(s) 112, 199, 742, 1118
BstMBI GATC 5 cut(s) 170, 351, 461, 550, 1203
BstMWI GCNNNNNNNGC 7 cut(s) 38, 71, 149, 182, 488, 930, 1136
BstNI CCWGG 2 cut(s) 377, 812
BstNSI RCATGY 1 cut(s) 1078
BstSCI CCNGG 2 cut(s) 375, 810
BstUI CGCG 4 cut(s) 71, 150, 168, 486
BstV1I GCAGC 4 cut(s) 139, 142, 309, 500
BstX2I RGATCY 3 cut(s) 170, 461, 1203
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 3 cut(s) 170, 461, 1203
BsuRI GGCC 2 cut(s) 165, 215
BtgI CCRYGG 2 cut(s) 166, 646
BtsCI GGATG 4 cut(s) 53, 820, 1161, 1189
BtsI GCAGTG 2 cut(s) 1204, 1216
BtsIMutI CAGTG 5 cut(s) 498, 709, 984, 1204, 1216
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 3 cut(s) 484, 926, 1192
CfoI GCGC 2 cut(s) 152, 488
Cfr13I GGNCC 3 cut(s) 214, 380, 734
Cfr42I CCGCGG 1 cut(s) 169
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 4 cut(s) 616, 953, 1075, 1156
DdeI CTNAG 7 cut(s) 128, 134, 393, 452, 501, 541, 1068
DpnI GATC 5 cut(s) 172, 353, 463, 552, 1205
DpnII GATC 5 cut(s) 170, 351, 461, 550, 1203
DriI GACNNNNNGTC 1 cut(s) 755
EaeI YGGCCR 1 cut(s) 163
Eam1105I GACNNNNNGTC 1 cut(s) 755
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 2 cut(s) 760, 862
Eco24I GRGCYC 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 199
Eco47I GGWCC 2 cut(s) 380, 734
Eco57I CTGAAG 2 cut(s) 438, 1122
EcoO109I RGGNCCY 2 cut(s) 214, 380
EcoRII CCWGG 2 cut(s) 375, 810
EcoT14I CCWWGG 2 cut(s) 760, 862
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 2 cut(s) 760, 862
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 4 cut(s) 619, 956, 1078, 1159
FaqI GGGAC 6 cut(s) 100, 320, 393, 526, 693, 952
FatI CATG 4 cut(s) 615, 952, 1074, 1155
FauI CCCGC 2 cut(s) 225, 775
FokI GGATG 4 cut(s) 40, 827, 1148, 1196
FriOI GRGCYC 1 cut(s) 453
FspBI CTAG 3 cut(s) 413, 659, 893
GlaI GCGC 2 cut(s) 151, 487
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 2 cut(s) 165, 215
HhaI GCGC 2 cut(s) 152, 488
Hin1II CATG 4 cut(s) 619, 956, 1078, 1159
Hin6I GCGC 2 cut(s) 150, 486
HinP1I GCGC 2 cut(s) 150, 486
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 4 cut(s) 407, 575, 965, 1007
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 265, 808
Hpy188I TCNGA 7 cut(s) 137, 396, 544, 574, 676, 977, 1102
Hpy188III TCNNGA 5 cut(s) 227, 278, 442, 454, 1053
Hpy8I GTNNAC 2 cut(s) 265, 808
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 9 cut(s) 31, 60, 103, 253, 297, 343, 451, 665, 1108
HpyCH4III ACNGT 2 cut(s) 650, 1027
HpyCH4V TGCA 6 cut(s) 11, 581, 670, 803, 1130, 1155
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 71, 149, 182, 488, 930, 1136
HpyF3I CTNAG 7 cut(s) 128, 134, 393, 452, 501, 541, 1068
Hsp92II CATG 4 cut(s) 619, 956, 1078, 1159
HspAI GCGC 2 cut(s) 150, 486
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 5 cut(s) 170, 351, 461, 550, 1203
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 4 cut(s) 139, 142, 309, 500
LweI GCATC 2 cut(s) 319, 351
MaeI CTAG 3 cut(s) 413, 659, 893
MaeIII GTNAC 4 cut(s) 275, 883, 911, 1036
MalI GATC 5 cut(s) 172, 353, 463, 552, 1205
MbiI CCGCTC 2 cut(s) 176, 220
MboI GATC 5 cut(s) 170, 351, 461, 550, 1203
MboII GAAGA 2 cut(s) 990, 1016
MflI RGATCY 3 cut(s) 170, 461, 1203
MhlI GDGCHC 2 cut(s) 187, 453
MluCI AATT 7 cut(s) 567, 592, 708, 786, 790, 957, 1044
MlyI GAGTC 1 cut(s) 974
MmeI TCCRAC 2 cut(s) 654, 1059
MroXI GAANNNNTTC 1 cut(s) 790
MseI TTAA 4 cut(s) 210, 474, 789, 1268
MslI CAYNNNNRTG 2 cut(s) 813, 836
MspA1I CMGCKG 3 cut(s) 168, 491, 993
MspR9I CCNGG 2 cut(s) 377, 812
Mva1269I GAATGC 1 cut(s) 1178
MvaI CCWGG 2 cut(s) 377, 812
MvnI CGCG 4 cut(s) 71, 150, 168, 486
MwoI GCNNNNNNNGC 7 cut(s) 38, 71, 149, 182, 488, 930, 1136
NdeII GATC 5 cut(s) 170, 351, 461, 550, 1203
NlaIII CATG 4 cut(s) 619, 956, 1078, 1159
NlaIV GGNNCC 7 cut(s) 78, 172, 216, 373, 381, 463, 735
NmuCI GTSAC 3 cut(s) 275, 883, 911
NspI RCATGY 1 cut(s) 1078
OliI CACNNNNGTG 1 cut(s) 836
PasI CCCWGGG 1 cut(s) 376
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1178
PdmI GAANNNNTTC 1 cut(s) 790
PfeI GAWTC 3 cut(s) 407, 575, 1007
PleI GAGTC 1 cut(s) 973
PpsI GAGTC 1 cut(s) 973
PpuMI RGGWCCY 1 cut(s) 380
PshBI ATTAAT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 1017
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 375, 810
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 2 cut(s) 375, 810
PspN4I GGNNCC 7 cut(s) 78, 172, 216, 373, 381, 463, 735
PspPI GGNCC 3 cut(s) 214, 380, 734
PspPPI RGGWCCY 1 cut(s) 380
PsuI RGATCY 3 cut(s) 170, 461, 1203
PvuII CAGCTG 1 cut(s) 491
RseI CAYNNNNRTG 2 cut(s) 813, 836
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 4 cut(s) 210, 474, 789, 1268
Sau3AI GATC 5 cut(s) 170, 351, 461, 550, 1203
Sau96I GGNCC 3 cut(s) 214, 380, 734
SchI GAGTC 1 cut(s) 974
ScrFI CCNGG 2 cut(s) 377, 812
SduI GDGCHC 2 cut(s) 187, 453
SfaNI GCATC 2 cut(s) 319, 351
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 2 cut(s) 380, 734
SmiMI CAYNNNNRTG 2 cut(s) 813, 836
SmlI CTYRAG 2 cut(s) 387, 400
SmoI CTYRAG 2 cut(s) 387, 400
Sse9I AATT 7 cut(s) 567, 592, 708, 786, 790, 957, 1044
SspI AATATT 2 cut(s) 433, 606
SspMI CTAG 3 cut(s) 413, 659, 893
StyD4I CCNGG 2 cut(s) 375, 810
StyI CCWWGG 2 cut(s) 760, 862
TaaI ACNGT 2 cut(s) 650, 1027
TaqI TCGA 1 cut(s) 234
TasI AATT 7 cut(s) 567, 592, 708, 786, 790, 957, 1044
TauI GCSGC 6 cut(s) 74, 77, 143, 168, 1197, 1231
TfiI GAWTC 3 cut(s) 407, 575, 1007
Tru1I TTAA 4 cut(s) 210, 474, 789, 1268
Tru9I TTAA 4 cut(s) 210, 474, 789, 1268
TscAI CASTG 5 cut(s) 505, 709, 991, 1204, 1216
TseFI GTSAC 3 cut(s) 275, 883, 911
TseI GCWGC 5 cut(s) 8, 152, 155, 322, 488
Tsp45I GTSAC 3 cut(s) 275, 883, 911
TspDTI ATGAA 6 cut(s) 42, 459, 492, 632, 941, 1185
TspGWI ACGGA 1 cut(s) 726
TspRI CASTG 5 cut(s) 505, 709, 991, 1204, 1216
VpaK11BI GGWCC 2 cut(s) 380, 734
VspI ATTAAT 1 cut(s) 789
XceI RCATGY 1 cut(s) 1078
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmnI GAANNNNTTC 1 cut(s) 790
XspI CTAG 3 cut(s) 413, 659, 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.