Rmu_sc0015288.1_g000001

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0015288.1
OG0008901
Physical Location & Seq
Forward (+)
761 .. 2093
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0015288.1_g000001.1.cds

Sequence Viewer

Length: 621 bp
atgtcggctgcaacttcctccttctctccagccctcaccgctactccaccttcatcctccaaatcccacgcggcggctccctccactctcgccttctcctccgccgtctccaagtccctcaaccctctcaggctcagagccgcctctcgtgctgctgctgatggccgcggatccgctctcggagctcgtatggtctcaatgccgacgattaagcccccgctctctcttgatttcgacacctctgttttcaacaaggagaaggtcaacctcgctggtcacgatgagtatattgtaaagggaggaagggacttgttccatctgctgcctgatgccttcaagggcatcaagcagatcggtgtcatcggttggggttcccagggacctgctcaagctcagaacttgagagattctctagctgaagcaaagtcaaatattgtggtcaagattgggctcaggaagggatcccgttcatttaatgaagctcgcgcagctggtttcactgaggagaatgggactttaggtgatatatgggaaacaatctcagagagtgatcttgtgttgctactaatctctgattctgcacaggtatgttggttctgcattggaaagttgtacatctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

206

Amino Acids

21.74

Weight (kDa)

9.17

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 391
AccBSI CCGCTC 2 cut(s) 176, 220
AccII CGCG 3 cut(s) 71, 168, 486
AciI CCGC 9 cut(s) 39, 71, 74, 102, 141, 166, 168, 174, 218
AclWI GGATC 4 cut(s) 165, 178, 456, 469
AcoI YGGCCR 1 cut(s) 163
AcuI CTGAAG 1 cut(s) 438
AfaI GTAC 1 cut(s) 614
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 2 cut(s) 250, 337
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 5 cut(s) 185, 392, 416, 482, 491
AluI AGCT 5 cut(s) 185, 392, 416, 482, 491
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 2 cut(s) 112, 199
AlwI GGATC 4 cut(s) 165, 178, 456, 469
AoxI GGCC 1 cut(s) 163
ApeKI GCWGC 5 cut(s) 8, 152, 155, 322, 488
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 28, 533
AvaII GGWCC 1 cut(s) 380
BamHI GGATCC 2 cut(s) 170, 461
BanII GRGCYC 2 cut(s) 187, 453
BauI CACGAG 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 187
BbvI GCAGC 4 cut(s) 139, 142, 309, 500
BccI CCATC 2 cut(s) 155, 324
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 377
BcoDI GTCTC 2 cut(s) 112, 199
BfaI CTAG 1 cut(s) 413
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 9 cut(s) 9, 72, 75, 141, 153, 156, 166, 323, 489
BlsI GCNGC 9 cut(s) 10, 73, 76, 142, 154, 157, 167, 324, 490
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 5 cut(s) 78, 172, 373, 381, 463
BmrFI CCNGG 1 cut(s) 377
BmsI GCATC 2 cut(s) 319, 351
BplI GAGNNNNNCTC 2 cut(s) 394, 426
BpmI CTGGAG 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 372, 421
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 3 cut(s) 166, 375, 376
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 1 cut(s) 73
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 3 cut(s) 166, 375, 376
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 6 cut(s) 142, 148, 407, 466, 492, 555
BseRI GAGGAG 2 cut(s) 88, 518
BseXI GCAGC 4 cut(s) 139, 142, 309, 500
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 3 cut(s) 71, 168, 486
BshFI GGCC 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 187
BslFI GGGAC 4 cut(s) 100, 320, 393, 526
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 2 cut(s) 112, 199
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 4 cut(s) 100, 320, 393, 526
BsnI GGCC 1 cut(s) 165
Bso31I GGTCTC 1 cut(s) 199
Bsp1286I GDGCHC 2 cut(s) 187, 453
Bsp1407I TGTACA 1 cut(s) 612
Bsp143I GATC 4 cut(s) 170, 351, 461, 550
BspACI CCGC 9 cut(s) 39, 71, 74, 102, 141, 166, 168, 174, 218
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 6 cut(s) 141, 147, 406, 465, 493, 554
BspFNI CGCG 3 cut(s) 71, 168, 486
BspLI GGNNCC 5 cut(s) 78, 172, 373, 381, 463
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 4 cut(s) 165, 178, 456, 469
BspTNI GGTCTC 1 cut(s) 199
BsrBI CCGCTC 2 cut(s) 176, 220
BsrGI TGTACA 1 cut(s) 612
BssECI CCNNGG 3 cut(s) 166, 375, 376
BssMI GATC 4 cut(s) 170, 351, 461, 550
BssSI CACGAG 1 cut(s) 147
Bst2BI CACGAG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 377
BstAUI TGTACA 1 cut(s) 612
BstC8I GCNNGC 1 cut(s) 484
BstDEI CTNAG 6 cut(s) 128, 134, 393, 452, 501, 541
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 3 cut(s) 71, 168, 486
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 4 cut(s) 173, 354, 464, 553
BstMAI GTCTC 2 cut(s) 112, 199
BstMBI GATC 4 cut(s) 170, 351, 461, 550
BstMWI GCNNNNNNNGC 4 cut(s) 38, 149, 182, 488
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 375
BstUI CGCG 3 cut(s) 71, 168, 486
BstV1I GCAGC 4 cut(s) 139, 142, 309, 500
BstX2I RGATCY 2 cut(s) 170, 461
BstYI RGATCY 2 cut(s) 170, 461
BsuRI GGCC 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 53
BtsIMutI CAGTG 1 cut(s) 498
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 484
CfoI GCGC 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 380
Cfr42I CCGCGG 1 cut(s) 169
Csp6I GTAC 1 cut(s) 613
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviQI GTAC 1 cut(s) 613
DdeI CTNAG 6 cut(s) 128, 134, 393, 452, 501, 541
DpnI GATC 4 cut(s) 172, 353, 463, 552
DpnII GATC 4 cut(s) 170, 351, 461, 550
EaeI YGGCCR 1 cut(s) 163
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 185
Eco24I GRGCYC 2 cut(s) 187, 453
Eco31I GGTCTC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 438
EcoICRI GAGCTC 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 380
EcoRII CCWGG 1 cut(s) 375
EcoT38I GRGCYC 2 cut(s) 187, 453
Esp3I CGTCTC 1 cut(s) 112
FaiI YATR 5 cut(s) 191, 288, 527, 529, 589
FaqI GGGAC 4 cut(s) 100, 320, 393, 526
FauI CCCGC 1 cut(s) 225
Fnu4HI GCNGC 9 cut(s) 9, 72, 75, 141, 153, 156, 166, 323, 489
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 2 cut(s) 187, 453
Fsp4HI GCNGC 9 cut(s) 9, 72, 75, 141, 153, 156, 166, 323, 489
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 487
GluI GCNGC 9 cut(s) 9, 72, 75, 141, 153, 156, 166, 323, 489
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 1 cut(s) 165
HhaI GCGC 1 cut(s) 488
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 2 cut(s) 407, 575
HphI GGTGA 2 cut(s) 28, 533
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 5 cut(s) 137, 182, 396, 544, 574
Hpy188III TCNNGA 4 cut(s) 227, 278, 442, 454
Hpy8I GTNNAC 1 cut(s) 265
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 7 cut(s) 31, 60, 103, 253, 297, 343, 451
HpyCH4V TGCA 3 cut(s) 11, 581, 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 149, 182, 488
HpyF3I CTNAG 6 cut(s) 128, 134, 393, 452, 501, 541
HspAI GCGC 1 cut(s) 486
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 4 cut(s) 170, 351, 461, 550
LmnI GCTCC 2 cut(s) 82, 182
Lsp1109I GCAGC 4 cut(s) 139, 142, 309, 500
LweI GCATC 2 cut(s) 319, 351
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 1 cut(s) 275
MalI GATC 4 cut(s) 172, 353, 463, 552
MbiI CCGCTC 2 cut(s) 176, 220
MboI GATC 4 cut(s) 170, 351, 461, 550
MflI RGATCY 2 cut(s) 170, 461
MhlI GDGCHC 2 cut(s) 187, 453
MseI TTAA 2 cut(s) 210, 474
MslI CAYNNNNRTG 1 cut(s) 586
MspA1I CMGCKG 2 cut(s) 168, 491
MspR9I CCNGG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 3 cut(s) 71, 168, 486
MwoI GCNNNNNNNGC 4 cut(s) 38, 149, 182, 488
NdeII GATC 4 cut(s) 170, 351, 461, 550
NlaIV GGNNCC 5 cut(s) 78, 172, 373, 381, 463
NmuCI GTSAC 1 cut(s) 275
PasI CCCWGGG 1 cut(s) 376
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PfeI GAWTC 2 cut(s) 407, 575
PkrI GCNGC 9 cut(s) 10, 73, 76, 142, 154, 157, 167, 324, 490
PpuMI RGGWCCY 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 187
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 5 cut(s) 78, 172, 373, 381, 463
PspPI GGNCC 1 cut(s) 380
PspPPI RGGWCCY 1 cut(s) 380
PsuI RGATCY 2 cut(s) 170, 461
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
RseI CAYNNNNRTG 1 cut(s) 586
SacI GAGCTC 1 cut(s) 187
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 2 cut(s) 210, 474
SatI GCNGC 9 cut(s) 9, 72, 75, 141, 153, 156, 166, 323, 489
Sau3AI GATC 4 cut(s) 170, 351, 461, 550
Sau96I GGNCC 1 cut(s) 380
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 2 cut(s) 187, 453
SfaNI GCATC 2 cut(s) 319, 351
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 586
SmlI CTYRAG 2 cut(s) 387, 400
SmoI CTYRAG 2 cut(s) 387, 400
SsiI CCGC 9 cut(s) 39, 71, 74, 102, 141, 166, 168, 174, 218
SspI AATATT 1 cut(s) 433
SspMI CTAG 1 cut(s) 413
SstI GAGCTC 1 cut(s) 187
StyD4I CCNGG 1 cut(s) 375
TaqI TCGA 1 cut(s) 234
TatI WGTACW 1 cut(s) 612
TauI GCSGC 4 cut(s) 74, 77, 143, 168
TfiI GAWTC 2 cut(s) 407, 575
Tru1I TTAA 2 cut(s) 210, 474
Tru9I TTAA 2 cut(s) 210, 474
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 5 cut(s) 8, 152, 155, 322, 488
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 42, 459, 492
TspRI CASTG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 380
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.