Rroxscaffold_3G00244700

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008901
Physical Location & Seq
Forward (+)
37528103 .. 37529430
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244700.1

Sequence Viewer

Length: 585 bp
ATGGCTCCACCTTCATCCTTTAAAGCCCACACGGCGGCTCCCTCCACTCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCGGGCTCATAGCCGCCTCTCGCGATGCGCTATTGGCCTCGGATCCGCTCTCGCAGCTCGTATGGTCTCAATGCCGACGATTAAGCCCCCGTTCTCTCTTGATTTCGACACCTCTCGTTTTCAACAAGGAGAAGATCAACCTCGCCGGTCACGATGAGTATATTGTAAAGGGAGAAAGGTACTTGTTCCATTCGCCGCCCGATGCCTTCAAGGGCATCAAGCAGATCGGTGTTATCGGTTGGGGTTTACAGGGACCTGCTCAAGCTCAGAACTTGAGAGATTCTCTAGCTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTCGGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACCGAGGAGAATGGGACTTTAGGTGACATATATATGGGAAACAATCTCGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCCGCACAGGTATGTTGGTTCTGCATTGGAAAGTCGTACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

194

Amino Acids

20.83

Weight (kDa)

8.79

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 103 - 183 8.9e-09 Acetohydroxy acid isomeroreductase, NADPH-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 352
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 2 cut(s) 111, 447
AciI CCGC 6 cut(s) 35, 63, 102, 134, 284, 543
AclWI GGATC 5 cut(s) 125, 138, 417, 430, 533
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 2 cut(s) 269, 578
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 2 cut(s) 211, 298
AluBI AGCT 5 cut(s) 145, 353, 377, 443, 452
AluI AGCT 5 cut(s) 145, 353, 377, 443, 452
Alw26I GTCTC 2 cut(s) 73, 159
AlwI GGATC 5 cut(s) 125, 138, 417, 430, 533
Ama87I CYCGRG 3 cut(s) 89, 413, 506
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 2 cut(s) 142, 449
AspLEI GCGC 2 cut(s) 118, 449
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 451, 494
AvaI CYCGRG 3 cut(s) 89, 413, 506
AvaII GGWCC 1 cut(s) 341
BamHI GGATCC 2 cut(s) 130, 422
BanII GRGCYC 2 cut(s) 96, 414
BbvI GCAGC 2 cut(s) 154, 461
BceAI ACGGC 2 cut(s) 48, 50
BcoDI GTCTC 2 cut(s) 73, 159
BfaI CTAG 1 cut(s) 374
BfuAI ACCTGC 1 cut(s) 352
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 5 cut(s) 36, 102, 143, 284, 450
BlsI GCNGC 5 cut(s) 37, 103, 144, 285, 451
Bme18I GGWCC 1 cut(s) 341
BmeT110I CYCGRG 3 cut(s) 89, 413, 506
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 5 cut(s) 6, 39, 132, 342, 424
BmsI GCATC 3 cut(s) 103, 280, 312
BplI GAGNNNNNCTC 2 cut(s) 355, 387
BpuEI CTTGAG 2 cut(s) 333, 382
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 126, 462
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 126, 462
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 368
BseRI GAGGAG 2 cut(s) 49, 479
BseXI GCAGC 2 cut(s) 154, 461
Bsh1236I CGCG 2 cut(s) 111, 447
BshFI GGCC 1 cut(s) 125
BsiHKCI CYCGRG 3 cut(s) 89, 413, 506
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 3 cut(s) 61, 354, 487
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 73, 159
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 3 cut(s) 61, 354, 487
BsnI GGCC 1 cut(s) 125
Bso31I GGTCTC 1 cut(s) 159
BsoBI CYCGRG 3 cut(s) 89, 413, 506
Bsp1286I GDGCHC 2 cut(s) 96, 414
Bsp143I GATC 6 cut(s) 130, 222, 312, 422, 514, 538
Bsp68I TCGCGA 1 cut(s) 111
BspACI CCGC 6 cut(s) 35, 63, 102, 134, 284, 543
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 367
BspFNI CGCG 2 cut(s) 111, 447
BspLI GGNNCC 5 cut(s) 6, 39, 132, 342, 424
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 5 cut(s) 125, 138, 417, 430, 533
BspTNI GGTCTC 1 cut(s) 159
BsrBI CCGCTC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 126, 462
BssMI GATC 6 cut(s) 130, 222, 312, 422, 514, 538
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 2 cut(s) 111, 447
BstHHI GCGC 2 cut(s) 118, 449
BstKTI GATC 6 cut(s) 133, 225, 315, 425, 517, 541
BstMAI GTCTC 2 cut(s) 73, 159
BstMBI GATC 6 cut(s) 130, 222, 312, 422, 514, 538
BstMWI GCNNNNNNNGC 4 cut(s) 32, 122, 142, 449
BstUI CGCG 2 cut(s) 111, 447
BstV1I GCAGC 2 cut(s) 154, 461
BstX2I RGATCY 2 cut(s) 130, 422
BstYI RGATCY 2 cut(s) 130, 422
BsuRI GGCC 1 cut(s) 125
BtgZI GCGATG 1 cut(s) 126
BtsCI GGATG 1 cut(s) 14
BtuMI TCGCGA 1 cut(s) 111
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 1 cut(s) 445
CfoI GCGC 2 cut(s) 118, 449
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 268, 577
CviQI GTAC 2 cut(s) 268, 577
DdeI CTNAG 1 cut(s) 354
DpnI GATC 6 cut(s) 132, 224, 314, 424, 516, 540
DpnII GATC 6 cut(s) 130, 222, 312, 422, 514, 538
DraI TTTAAA 1 cut(s) 22
EciI GGCGGA 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 96, 414
Eco31I GGTCTC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 3 cut(s) 89, 413, 506
EcoO109I RGGNCCY 1 cut(s) 341
EcoT38I GRGCYC 2 cut(s) 96, 414
Esp3I CGTCTC 1 cut(s) 73
FaiI YATR 8 cut(s) 98, 151, 249, 488, 490, 492, 494, 553
FaqI GGGAC 3 cut(s) 61, 354, 487
FauI CCCGC 1 cut(s) 550
Fnu4HI GCNGC 5 cut(s) 36, 102, 143, 284, 450
FriOI GRGCYC 2 cut(s) 96, 414
Fsp4HI GCNGC 5 cut(s) 36, 102, 143, 284, 450
FspBI CTAG 1 cut(s) 374
GlaI GCGC 2 cut(s) 117, 448
GluI GCNGC 5 cut(s) 36, 102, 143, 284, 450
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 234
HhaI GCGC 2 cut(s) 118, 449
Hin6I GCGC 2 cut(s) 116, 447
HinP1I GCGC 2 cut(s) 116, 447
HinfI GANTC 1 cut(s) 368
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 451, 494
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 3 cut(s) 130, 357, 538
Hpy188III TCNNGA 7 cut(s) 110, 187, 239, 403, 415, 506, 508
Hpy8I GTNNAC 1 cut(s) 335
Hpy99I CGWCG 1 cut(s) 168
HpyAV CCTTC 4 cut(s) 21, 64, 304, 412
HpyCH4V TGCA 1 cut(s) 564
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 122, 142, 449
HpyF3I CTNAG 1 cut(s) 354
HspAI GCGC 2 cut(s) 116, 447
Kzo9I GATC 6 cut(s) 130, 222, 312, 422, 514, 538
LmnI GCTCC 2 cut(s) 10, 43
LpnPI CCDG 5 cut(s) 247, 323, 357, 438, 533
Lsp1109I GCAGC 2 cut(s) 154, 461
LweI GCATC 3 cut(s) 103, 280, 312
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 236, 482
MalI GATC 6 cut(s) 132, 224, 314, 424, 516, 540
MbiI CCGCTC 1 cut(s) 136
MboI GATC 6 cut(s) 130, 222, 312, 422, 514, 538
MboII GAAGA 1 cut(s) 232
MflI RGATCY 2 cut(s) 130, 422
MhlI GDGCHC 2 cut(s) 96, 414
MluCI AATT 1 cut(s) 531
MnlI CCTC 9 cut(s) 52, 70, 89, 96, 115, 136, 210, 239, 457
MseI TTAA 3 cut(s) 21, 170, 435
MslI CAYNNNNRTG 2 cut(s) 491, 550
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 234
MvnI CGCG 2 cut(s) 111, 447
MwoI GCNNNNNNNGC 4 cut(s) 32, 122, 142, 449
NdeII GATC 6 cut(s) 130, 222, 312, 422, 514, 538
NlaIV GGNNCC 5 cut(s) 6, 39, 132, 342, 424
NmuCI GTSAC 2 cut(s) 236, 482
NruI TCGCGA 1 cut(s) 111
PaeR7I CTCGAG 1 cut(s) 506
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 5 cut(s) 37, 103, 144, 285, 451
PpuMI RGGWCCY 1 cut(s) 341
Psp5II RGGWCCY 1 cut(s) 341
PspN4I GGNNCC 5 cut(s) 6, 39, 132, 342, 424
PspPI GGNCC 1 cut(s) 341
PspPPI RGGWCCY 1 cut(s) 341
PsuI RGATCY 2 cut(s) 130, 422
PvuII CAGCTG 1 cut(s) 452
RruI TCGCGA 1 cut(s) 111
RsaI GTAC 2 cut(s) 269, 578
RsaNI GTAC 2 cut(s) 268, 577
RseI CAYNNNNRTG 2 cut(s) 491, 550
SaqAI TTAA 3 cut(s) 21, 170, 435
SatI GCNGC 5 cut(s) 36, 102, 143, 284, 450
Sau3AI GATC 6 cut(s) 130, 222, 312, 422, 514, 538
Sau96I GGNCC 1 cut(s) 341
SduI GDGCHC 2 cut(s) 96, 414
SfaNI GCATC 3 cut(s) 103, 280, 312
Sfr274I CTCGAG 1 cut(s) 506
SinI GGWCC 1 cut(s) 341
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 491, 550
SmlI CTYRAG 3 cut(s) 348, 361, 506
SmoI CTYRAG 3 cut(s) 348, 361, 506
Sse9I AATT 1 cut(s) 531
SsiI CCGC 6 cut(s) 35, 63, 102, 134, 284, 543
SspI AATATT 1 cut(s) 394
SspMI CTAG 1 cut(s) 374
TaqI TCGA 3 cut(s) 194, 389, 507
TasI AATT 1 cut(s) 531
TauI GCSGC 3 cut(s) 38, 104, 286
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 3 cut(s) 21, 170, 435
Tru9I TTAA 3 cut(s) 21, 170, 435
TseFI GTSAC 2 cut(s) 236, 482
TseI GCWGC 2 cut(s) 142, 449
Tsp45I GTSAC 2 cut(s) 236, 482
TspDTI ATGAA 2 cut(s) 420, 453
VpaK11BI GGWCC 1 cut(s) 341
XhoI CTCGAG 1 cut(s) 506
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.