Rh7CG194400

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008901
Physical Location & Seq
Reverse (-)
17099383 .. 17116665
17283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG194400.1

Sequence Viewer

Length: 2346 bp
ATGGGTTCGTCAGGGATGGCTCCACCTTCATCCTCTACTCCACCTTCATCCTCTAAAGCCCACGCAGCGGCTCCCTCCACTCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCAGAGCCGCCTCTCGCGGTGCTGCTATTGGCCGCGGATCCGCTCTCAGAGCTCGTATGGTCTCAATGCCGACGATTAAGCCCCCGCTCTCTCTTGATTTCGACACCTCTGTTTTCAACAAGGAGAAGATCAACCTCGCTGGTCACGATGAGTATATTGTAAAGGGAGGAAGGTACTTGTTCCATCTGCTGCCTGATGCCTTCAAGGGCATCAAGCAGATCGGTGTTATCAGTTGGGGTTCACAGGGACCTGCTCAAGCTCAGAACTTAAGAGATTCTCTAGCTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACCGAGGAGAATGGGACTTTAGGTGACATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCTGCACAGGCAGATAATTATGAGAAAATATTTTCGCACATGAAACCAAATAGCATATTTGGTCTTTCCCACGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTCAGAAGATACACCGACAGCGGAATGAGTGAAGATTCTGCTTATAAAAATACTATTGAATGTGTTACTGGAATTGTGTCAAGAACCATATCAACTAAGGGCATGTTGGAAATATACAGCTCTTTGTCTGAAGCTGACAAGAAGGAGTTTGAGACTGCATATAGTGCCTCCTATTATCCTTGCATGGACATCCTCTATGAATGCTATGAGGATGTAGCAAGCGGCAGTGAGATCCGTAGCATTGTTTTGGCTGGGCGGCGATTTTATGGAGCTGTTGAACGTTGTAGTGTTTCAAAACGTCGGAAACTTAACTGGGAATTGCCTGTTACTGAAGTGCAAATGACCGATTCACCTGATTCGACGGTGGTACGTAAGGACGGAGAGAAAGTGGGTATCGAGGCAGAGATGATTTCGGGTACTTCGGAGTTGTTCAATGTGCCAGTTGTAGCTGCGGGTTTTGGGGTTGAGAATGTCCCACTGGAATCATCAGAAAAGGGGGCGAAATTGACACATGAGGAAGTGAAAGCAATGGAAAAGAAAGACGGGTTCCTGCATGCTCATCCAGAGAGGCTTCTGCCTTTGCAAACTACTCATAGTCCCAATTTCTTGGGGCTGCAACAAGGGTTCGGACTTTGGAAAGGGACTAATTACGGGGAAGGTGTGATCATTGGAGTTTTGGATATTGGAATATCTCCAGATCATCCCTCATTTAGTGATGATGGAGTGCCACATCCCCTAGCGAAATGGAAAGGCAAGTGCGAGTTCAATGGGACAGCCATCTACAAGGTCTGCACCGAGGCAAGCTGTGCCGAAAGTGACATGCTAGTAGCTCTTGACACTGCTGTTGAGGACGGTGTGGATGTGCTCTCACTGTCCATAGGTGCAAATCCCAATAACCAAACTGAGTTCTGTGCCTCAGGATCTCTTGTAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGAGAGGTGGAGGAGTGGGAAGAATTGCAGAAGGGGAAGAAGTGAAAAGAGCAGGAGGTGCTGCCATGATTCTAGTGAACCAAATCACTAATGACTATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGAGATGCACTTGCTCCGATGGTTGCTTCTTTCTCATCTAGAGGACAGAGCATAGCTAGCCCCGGAATTTTGAAACCTGACATCATTGGTCCTGGTGTTAACATGCTCGCTGCATGGCCTGTTTCTGTGGATAATGTCACAGCATTTAACATGATTTCAGGTACCTCAATGTCATGCCCACACCTAAGTGGCATTCCAGCCTTGATCAAGAGTTCACACCCATATTGGTCACCTGCTGCCATCAAATCCACCATTATGACAACCGCTGATATGCTAAACCTCAAAGGCACTCCAATTGTTGATAGATTAACTGTCCCAGCAGAAATCTATAGCACTGGCTCAAGCCATGTCAACCCTTCAAAAGCCAATGACCCGGGGCTCATCTATGACACAAAACCAGAAGATTACATTCCTTACTTGTGTGGTCTGAACTACACAGACAATCAAATAGAGATAATCACCCAACAAAGAGTAAACTGCTCTGAAGTAGGAATCACAGCTGAAGCTCAAACTCAATTACACCAGAATTGTGACAAATGTTGGCCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

781

Amino Acids

83.47

Weight (kDa)

5.45

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 114 - 216 4.6e-14 Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C PF01450 214 - 317 8.3e-21 Ketol-acid reductoisomerase, C-terminal domain
Peptidase_S8 PF00082 487 - 690 5.3e-22 Subtilase family
PA PF02225 530 - 602 2.1e-09 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AarI CACCTGC 1 cut(s) 2035
Acc36I ACCTGC 2 cut(s) 385, 2035
Acc65I GGTACC 1 cut(s) 1955
AccB1I GGYRCC 1 cut(s) 1955
AccBSI CCGCTC 2 cut(s) 170, 214
AccII CGCG 3 cut(s) 144, 162, 480
AclI AACGTT 1 cut(s) 964
AclWI GGATC 7 cut(s) 159, 172, 450, 463, 563, 910, 1612
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 1860
AcuI CTGAAG 5 cut(s) 432, 834, 1035, 2298, 2316
AfaI GTAC 5 cut(s) 302, 1053, 1102, 1764, 1957
AfiI CCNNNNNNNGG 1 cut(s) 67
AflII CTTAAG 1 cut(s) 394
AjnI CCWGG 1 cut(s) 1885
AleI CACNNNNGTG 1 cut(s) 1980
AloI GAACNNNNNNTCC 2 cut(s) 668, 700
Alw21I GWGCWC 2 cut(s) 181, 1551
Alw26I GTCTC 3 cut(s) 106, 193, 830
AlwI GGATC 7 cut(s) 159, 172, 450, 463, 563, 910, 1612
Ama87I CYCGRG 1 cut(s) 2167
AoxI GGCC 3 cut(s) 157, 1910, 2336
ApeKI GCWGC 9 cut(s) 65, 149, 316, 482, 1133, 1297, 1703, 1904, 2030
ApoI RAATTY 1 cut(s) 1860
Asp718I GGTACC 1 cut(s) 1955
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 3 cut(s) 374, 1883, 2337
AsuC2I CCSGG 4 cut(s) 1857, 2168, 2169, 2340
AsuHPI GGTGA 5 cut(s) 484, 527, 1026, 2016, 2245
AsuNHI GCTAGC 1 cut(s) 1850
AvaI CYCGRG 1 cut(s) 2167
AvaII GGWCC 2 cut(s) 374, 1883
AxyI CCTNAGG 1 cut(s) 1600
BamHI GGATCC 2 cut(s) 164, 455
BanI GGYRCC 1 cut(s) 1955
BanII GRGCYC 3 cut(s) 181, 447, 2175
Bbv12I GWGCWC 2 cut(s) 181, 1551
BbvI GCAGC 9 cut(s) 77, 136, 303, 494, 1120, 1284, 1690, 1891, 2017
BccI CCATC 6 cut(s) 10, 318, 1397, 1469, 1807, 2042
BceAI ACGGC 2 cut(s) 83, 658
BciT130I CCWGG 1 cut(s) 1887
BclI TGATCA 2 cut(s) 1347, 1998
BcnI CCSGG 4 cut(s) 1857, 2168, 2169, 2340
BcoDI GTCTC 3 cut(s) 106, 193, 830
BfaI CTAG 6 cut(s) 407, 1421, 1508, 1715, 1833, 1851
BfmI CTRYAG 2 cut(s) 1738, 2122
BfrI CTTAAG 1 cut(s) 394
BfuAI ACCTGC 2 cut(s) 385, 2035
Bme1390I CCNGG 5 cut(s) 1857, 1887, 2168, 2169, 2340
Bme18I GGWCC 2 cut(s) 374, 1883
BmeT110I CYCGRG 1 cut(s) 2167
BmgT120I GGNCC 3 cut(s) 374, 1883, 2337
BmiI GGNNCC 7 cut(s) 21, 72, 166, 375, 457, 1232, 1957
BmrFI CCNGG 5 cut(s) 1857, 1887, 2168, 2169, 2340
BmrI ACTGGG 1 cut(s) 1006
BmsI GCATC 4 cut(s) 313, 345, 1744, 1789
BmtI GCTAGC 1 cut(s) 1854
BmuI ACTGGG 1 cut(s) 1006
BpmI CTGGAG 1 cut(s) 1362
Bpu10I CCTNAGC 2 cut(s) 446, 1747
BpuEI CTTGAG 2 cut(s) 366, 2119
BpuMI CCSGG 4 cut(s) 1857, 2168, 2169, 2340
BsaAI YACGTR 1 cut(s) 1055
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 7 cut(s) 160, 495, 640, 1479, 1855, 2167, 2168
BsaXI ACNNNNNCTCC 4 cut(s) 668, 698, 1056, 1086
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 5 cut(s) 757, 1001, 1124, 1167, 2134
Bse21I CCTNAGG 1 cut(s) 1600
Bse3DI GCAATG 1 cut(s) 1218
BseBI CCWGG 1 cut(s) 1887
BseDI CCNNGG 7 cut(s) 160, 495, 640, 1479, 1855, 2167, 2168
BseGI GGATG 9 cut(s) 21, 29, 47, 873, 901, 1243, 1384, 1414, 1549
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 1218
BseMII CTCAG 8 cut(s) 136, 142, 187, 401, 460, 549, 1578, 1614
BseNI ACTGG 5 cut(s) 757, 1001, 1124, 1167, 2134
BseRI GAGGAG 3 cut(s) 82, 512, 1668
BseXI GCAGC 9 cut(s) 77, 136, 303, 494, 1120, 1284, 1690, 1891, 2017
BseYI CCCAGC 2 cut(s) 935, 2110
BsgI GTGCAG 2 cut(s) 558, 1459
Bsh1236I CGCG 3 cut(s) 144, 162, 480
BshFI GGCC 3 cut(s) 159, 1912, 2338
BshNI GGYRCC 1 cut(s) 1955
BsiHKAI GWGCWC 2 cut(s) 181, 1551
BsiHKCI CYCGRG 1 cut(s) 2167
BsiSI CCGG 3 cut(s) 1857, 2168, 2340
BslFI GGGAC 9 cut(s) 94, 387, 520, 664, 1142, 1266, 1339, 1468, 2093
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 3 cut(s) 106, 193, 830
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 9 cut(s) 94, 387, 520, 664, 1142, 1266, 1339, 1468, 2093
BsmI GAATGC 2 cut(s) 890, 1986
BsnI GGCC 3 cut(s) 159, 1912, 2338
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 2167
Bsp1286I GDGCHC 4 cut(s) 181, 447, 1551, 2175
BspANI GGCC 3 cut(s) 159, 1912, 2338
BspCNI CTCAG 8 cut(s) 135, 141, 186, 400, 459, 548, 1579, 1613
BspFNI CGCG 3 cut(s) 144, 162, 480
BspLI GGNNCC 7 cut(s) 21, 72, 166, 375, 457, 1232, 1957
BspMI ACCTGC 2 cut(s) 385, 2035
BspOI GCTAGC 1 cut(s) 1854
BspPI GGATC 7 cut(s) 159, 172, 450, 463, 563, 910, 1612
BspT107I GGYRCC 1 cut(s) 1955
BspTI CTTAAG 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 193
BsrBI CCGCTC 2 cut(s) 170, 214
BsrDI GCAATG 1 cut(s) 1218
BsrI ACTGG 5 cut(s) 757, 1001, 1124, 1167, 2134
BssECI CCNNGG 7 cut(s) 160, 495, 640, 1479, 1855, 2167, 2168
Bst2UI CCWGG 1 cut(s) 1887
Bst4CI ACNGT 4 cut(s) 1048, 1538, 1557, 2107
BstAFI CTTAAG 1 cut(s) 394
BstAPI GCANNNNNTGC 4 cut(s) 848, 1490, 1700, 1799
BstBAI YACGTR 1 cut(s) 1055
BstC8I GCNNGC 6 cut(s) 478, 904, 1239, 1486, 1852, 1902
BstDSI CCRYGG 2 cut(s) 160, 640
BstEII GGTNACC 1 cut(s) 2022
BstF5I GGATG 9 cut(s) 21, 29, 47, 873, 901, 1243, 1384, 1414, 1549
BstFNI CGCG 3 cut(s) 144, 162, 480
BstHHI GCGC 1 cut(s) 482
BstMAI GTCTC 3 cut(s) 106, 193, 830
BstMWI GCNNNNNNNGC 8 cut(s) 65, 176, 482, 848, 1490, 1700, 1799, 1851
BstNI CCWGG 1 cut(s) 1887
BstNSI RCATGY 4 cut(s) 790, 1241, 1507, 1900
BstPI GGTNACC 1 cut(s) 2022
BstSCI CCNGG 5 cut(s) 1855, 1885, 2166, 2167, 2338
BstSFI CTRYAG 2 cut(s) 1738, 2122
BstSNI TACGTA 1 cut(s) 1055
BstUI CGCG 3 cut(s) 144, 162, 480
BstV1I GCAGC 9 cut(s) 77, 136, 303, 494, 1120, 1284, 1690, 1891, 2017
BstX2I RGATCY 4 cut(s) 164, 455, 915, 1604
BstXI CCANNNNNNTGG 1 cut(s) 1291
BstYI RGATCY 4 cut(s) 164, 455, 915, 1604
Bsu36I CCTNAGG 1 cut(s) 1600
BsuRI GGCC 3 cut(s) 159, 1912, 2338
BtgI CCRYGG 2 cut(s) 160, 640
BtsCI GGATG 9 cut(s) 21, 29, 47, 873, 901, 1243, 1384, 1414, 1549
BtsI GCAGTG 2 cut(s) 916, 1521
BtsIMutI CAGTG 5 cut(s) 916, 1160, 1521, 1553, 2127
BveI ACCTGC 2 cut(s) 385, 2035
Cac8I GCNNGC 6 cut(s) 478, 904, 1239, 1486, 1852, 1902
CfoI GCGC 1 cut(s) 482
Cfr13I GGNCC 3 cut(s) 374, 1883, 2337
Cfr42I CCGCGG 1 cut(s) 163
Cfr9I CCCGGG 1 cut(s) 2167
Csp6I GTAC 5 cut(s) 301, 1052, 1101, 1763, 1956
CviQI GTAC 5 cut(s) 301, 1052, 1101, 1763, 1956
EaeI YGGCCR 1 cut(s) 157
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 179
Eco105I TACGTA 1 cut(s) 1055
Eco24I GRGCYC 3 cut(s) 181, 447, 2175
Eco31I GGTCTC 1 cut(s) 193
Eco47I GGWCC 2 cut(s) 374, 1883
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 5 cut(s) 432, 834, 1035, 2298, 2316
Eco81I CCTNAGG 1 cut(s) 1600
Eco88I CYCGRG 1 cut(s) 2167
Eco91I GGTNACC 1 cut(s) 2022
EcoICRI GAGCTC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 374
EcoO65I GGTNACC 1 cut(s) 2022
EcoRII CCWGG 1 cut(s) 1885
EcoT38I GRGCYC 3 cut(s) 181, 447, 2175
Esp3I CGTCTC 1 cut(s) 106
FaqI GGGAC 9 cut(s) 94, 387, 520, 664, 1142, 1266, 1339, 1468, 2093
FauI CCCGC 2 cut(s) 219, 1129
FauNDI CATATG 1 cut(s) 521
FbaI TGATCA 2 cut(s) 1347, 1998
FokI GGATG 9 cut(s) 16, 28, 34, 860, 908, 1230, 1371, 1401, 1556
FriOI GRGCYC 3 cut(s) 181, 447, 2175
FspBI CTAG 6 cut(s) 407, 1421, 1508, 1715, 1833, 1851
GlaI GCGC 1 cut(s) 481
GsaI CCCAGC 2 cut(s) 939, 2114
GsuI CTGGAG 1 cut(s) 1362
HaeIII GGCC 3 cut(s) 159, 1912, 2338
HapII CCGG 3 cut(s) 1857, 2168, 2340
HhaI GCGC 1 cut(s) 482
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HincII GTYRAC 2 cut(s) 1894, 2146
HindII GTYRAC 2 cut(s) 1894, 2146
HinfI GANTC 8 cut(s) 401, 677, 719, 1031, 1040, 1166, 1711, 2286
HpaI GTTAAC 1 cut(s) 1894
HpaII CCGG 3 cut(s) 1857, 2168, 2340
HphI GGTGA 5 cut(s) 484, 527, 1026, 2016, 2245
Hpy166II GTNNAC 6 cut(s) 368, 1720, 1894, 2009, 2146, 2269
Hpy8I GTNNAC 6 cut(s) 368, 1720, 1894, 2009, 2146, 2269
Hpy99I CGWCG 3 cut(s) 202, 987, 1048
HpyCH4III ACNGT 4 cut(s) 1048, 1538, 1557, 2107
HpyCH4IV ACGT 3 cut(s) 964, 982, 1054
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 176, 482, 848, 1490, 1700, 1799, 1851
HpySE526I ACGT 3 cut(s) 964, 982, 1054
HspAI GCGC 1 cut(s) 480
KpnI GGTACC 1 cut(s) 1959
Ksp22I TGATCA 2 cut(s) 1347, 1998
KspAI GTTAAC 1 cut(s) 1894
KspI CCGCGG 1 cut(s) 163
LmnI GCTCC 4 cut(s) 25, 76, 953, 1813
Lsp1109I GCAGC 9 cut(s) 77, 136, 303, 494, 1120, 1284, 1690, 1891, 2017
LweI GCATC 4 cut(s) 313, 345, 1744, 1789
MaeI CTAG 6 cut(s) 407, 1421, 1508, 1715, 1833, 1851
MaeII ACGT 3 cut(s) 964, 982, 1054
MaeIII GTNAC 8 cut(s) 269, 515, 748, 1009, 1499, 1930, 2022, 2324
MbiI CCGCTC 2 cut(s) 170, 214
MboII GAAGA 6 cut(s) 265, 702, 728, 1674, 1691, 2207
MfeI CAATTG 1 cut(s) 2088
MflI RGATCY 4 cut(s) 164, 455, 915, 1604
MhlI GDGCHC 4 cut(s) 181, 447, 1551, 2175
MlyI GAGTC 1 cut(s) 686
MmeI TCCRAC 2 cut(s) 771, 965
MseI TTAA 7 cut(s) 204, 395, 468, 993, 1893, 1941, 2102
MslI CAYNNNNRTG 2 cut(s) 1980, 2048
MspA1I CMGCKG 6 cut(s) 68, 162, 485, 705, 2060, 2294
MspCI CTTAAG 1 cut(s) 394
MspI CCGG 3 cut(s) 1857, 2168, 2340
MspR9I CCNGG 5 cut(s) 1857, 1887, 2168, 2169, 2340
MunI CAATTG 1 cut(s) 2088
Mva1269I GAATGC 2 cut(s) 890, 1986
MvaI CCWGG 1 cut(s) 1887
MvnI CGCG 3 cut(s) 144, 162, 480
MwoI GCNNNNNNNGC 8 cut(s) 65, 176, 482, 848, 1490, 1700, 1799, 1851
NciI CCSGG 4 cut(s) 1857, 2168, 2169, 2340
NdeI CATATG 1 cut(s) 521
NheI GCTAGC 1 cut(s) 1850
NlaIV GGNNCC 7 cut(s) 21, 72, 166, 375, 457, 1232, 1957
NmuCI GTSAC 6 cut(s) 269, 515, 1499, 1930, 2022, 2324
NspI RCATGY 4 cut(s) 790, 1241, 1507, 1900
OliI CACNNNNGTG 1 cut(s) 1980
PaeI GCATGC 1 cut(s) 1241
PaqCI CACCTGC 1 cut(s) 2035
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 2 cut(s) 890, 1986
PfeI GAWTC 7 cut(s) 401, 719, 1031, 1040, 1166, 1711, 2286
PleI GAGTC 1 cut(s) 685
PpsI GAGTC 1 cut(s) 685
Ppu21I YACGTR 1 cut(s) 1055
PpuMI RGGWCCY 1 cut(s) 374
PsiI TTATAA 1 cut(s) 729
Psp124BI GAGCTC 1 cut(s) 181
Psp1406I AACGTT 1 cut(s) 964
Psp5II RGGWCCY 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 1885
PspEI GGTNACC 1 cut(s) 2022
PspFI CCCAGC 2 cut(s) 935, 2110
PspGI CCWGG 1 cut(s) 1885
PspN4I GGNNCC 7 cut(s) 21, 72, 166, 375, 457, 1232, 1957
PspPI GGNCC 3 cut(s) 374, 1883, 2337
PspPPI RGGWCCY 1 cut(s) 374
PsuI RGATCY 4 cut(s) 164, 455, 915, 1604
PvuII CAGCTG 2 cut(s) 485, 2294
RsaI GTAC 5 cut(s) 302, 1053, 1102, 1764, 1957
RsaNI GTAC 5 cut(s) 301, 1052, 1101, 1763, 1956
RseI CAYNNNNRTG 2 cut(s) 1980, 2048
SacI GAGCTC 1 cut(s) 181
SacII CCGCGG 1 cut(s) 163
SaqAI TTAA 7 cut(s) 204, 395, 468, 993, 1893, 1941, 2102
Sau96I GGNCC 3 cut(s) 374, 1883, 2337
SchI GAGTC 1 cut(s) 686
ScrFI CCNGG 5 cut(s) 1857, 1887, 2168, 2169, 2340
SduI GDGCHC 4 cut(s) 181, 447, 1551, 2175
SfaNI GCATC 4 cut(s) 313, 345, 1744, 1789
SfcI CTRYAG 2 cut(s) 1738, 2122
Sfr303I CCGCGG 1 cut(s) 163
SgrBI CCGCGG 1 cut(s) 163
SinI GGWCC 2 cut(s) 374, 1883
SmaI CCCGGG 1 cut(s) 2169
SmiMI CAYNNNNRTG 2 cut(s) 1980, 2048
SmlI CTYRAG 3 cut(s) 381, 394, 2134
SmoI CTYRAG 3 cut(s) 381, 394, 2134
SnaBI TACGTA 1 cut(s) 1055
SphI GCATGC 1 cut(s) 1241
SspI AATATT 2 cut(s) 427, 600
SspMI CTAG 6 cut(s) 407, 1421, 1508, 1715, 1833, 1851
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 5 cut(s) 1855, 1885, 2166, 2167, 2338
TaaI ACNGT 4 cut(s) 1048, 1538, 1557, 2107
TaiI ACGT 3 cut(s) 967, 985, 1057
TaqI TCGA 4 cut(s) 228, 422, 1043, 1080
TaqII GACCGA 1 cut(s) 1043
TatI WGTACW 1 cut(s) 1762
TauI GCSGC 5 cut(s) 71, 137, 162, 909, 943
TfiI GAWTC 7 cut(s) 401, 719, 1031, 1040, 1166, 1711, 2286
Tru1I TTAA 7 cut(s) 204, 395, 468, 993, 1893, 1941, 2102
Tru9I TTAA 7 cut(s) 204, 395, 468, 993, 1893, 1941, 2102
TscAI CASTG 5 cut(s) 916, 1167, 1528, 1560, 2134
TseFI GTSAC 6 cut(s) 269, 515, 1499, 1930, 2022, 2324
TseI GCWGC 9 cut(s) 65, 149, 316, 482, 1133, 1297, 1703, 1904, 2030
Tsp45I GTSAC 6 cut(s) 269, 515, 1499, 1930, 2022, 2324
TspDTI ATGAA 7 cut(s) 18, 36, 453, 486, 626, 653, 897
TspGWI ACGGA 2 cut(s) 908, 1077
TspMI CCCGGG 1 cut(s) 2167
TspRI CASTG 5 cut(s) 916, 1167, 1528, 1560, 2134
Vha464I CTTAAG 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 374, 1883
XapI RAATTY 1 cut(s) 1860
XbaI TCTAGA 1 cut(s) 1832
XceI RCATGY 4 cut(s) 790, 1241, 1507, 1900
XmaI CCCGGG 1 cut(s) 2167
XspI CTAG 6 cut(s) 407, 1421, 1508, 1715, 1833, 1851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.