Rh1CG226700

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0008901
Physical Location & Seq
Forward (+)
48018362 .. 48019121
760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG226700.1

Sequence Viewer

Length: 372 bp
ATGCCTTTCCGGTTCAGAATTTTCTCCAAACAGTGCCATGTGGATTTCGTCATCACCATTTCTATCAACCTGGATGAGCAGCTGATGGGTTCGTCAGGGATGGCTCCACCTTCATCCTCTAAAGCCCACGCGGCGGCTCCCTCCACTCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCAGAGCCGCCTCTCGCGGTGCTGCTATTGGCCGCGGATCCGCTCTCAGAGCTCGTATGGTCTCAATGCCGACGATTAAGCCCCCGCTTTCTCTTGATTTCGACACCTCTAGTATTCATAGTGATTTGAGATATGTATATTGTAAAGGGAGAAAGGTACTTGTTCCATCTGCCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

123

Amino Acids

13.18

Weight (kDa)

10.67

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 236
AccII CGCG 3 cut(s) 131, 210, 228
AclWI GGATC 2 cut(s) 225, 238
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 18
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 133
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 2 cut(s) 82, 245
AluI AGCT 2 cut(s) 82, 245
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 2 cut(s) 172, 259
AlwI GGATC 2 cut(s) 225, 238
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 2 cut(s) 79, 215
ApoI RAATTY 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 46
BamHI GGATCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 2 cut(s) 91, 202
BccI CCATC 3 cut(s) 79, 94, 367
BceAI ACGGC 1 cut(s) 149
BciT130I CCWGG 1 cut(s) 71
BcoDI GTCTC 2 cut(s) 172, 259
BfaI CTAG 1 cut(s) 303
BglI GCCNNNNNGGC 1 cut(s) 131
BisI GCNGC 7 cut(s) 80, 132, 135, 201, 216, 226, 366
BlsI GCNGC 7 cut(s) 81, 133, 136, 202, 217, 227, 367
Bme1390I CCNGG 1 cut(s) 71
BmiI GGNNCC 3 cut(s) 105, 138, 232
BmrFI CCNGG 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 3 cut(s) 79, 105, 113
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 3 cut(s) 202, 208, 253
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 2 cut(s) 91, 202
Bsh1236I CGCG 3 cut(s) 131, 210, 228
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 2 cut(s) 172, 259
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 1 cut(s) 225
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 1 cut(s) 230
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 3 cut(s) 201, 207, 252
BspFNI CGCG 3 cut(s) 131, 210, 228
BspLI GGNNCC 3 cut(s) 105, 138, 232
BspPI GGATC 2 cut(s) 225, 238
BspTNI GGTCTC 1 cut(s) 259
BsrBI CCGCTC 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 33
BstDEI CTNAG 3 cut(s) 188, 194, 239
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 3 cut(s) 79, 105, 113
BstFNI CGCG 3 cut(s) 131, 210, 228
BstKTI GATC 1 cut(s) 233
BstMAI GTCTC 2 cut(s) 172, 259
BstMBI GATC 1 cut(s) 230
BstMWI GCNNNNNNNGC 2 cut(s) 131, 242
BstNI CCWGG 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 69
BstUI CGCG 3 cut(s) 131, 210, 228
BstV1I GCAGC 2 cut(s) 91, 202
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 3 cut(s) 79, 105, 113
BtsIMutI CAGTG 1 cut(s) 38
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 1 cut(s) 350
CviAII CATG 1 cut(s) 38
CviJI RGCY 9 cut(s) 82, 104, 125, 137, 193, 200, 225, 245, 274
CviKI_1 RGCY 9 cut(s) 82, 104, 125, 137, 193, 200, 225, 245, 274
CviQI GTAC 1 cut(s) 350
DdeI CTNAG 3 cut(s) 188, 194, 239
DpnI GATC 1 cut(s) 232
DpnII GATC 1 cut(s) 230
EaeI YGGCCR 1 cut(s) 223
EciI GGCGGA 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 259
Eco53kI GAGCTC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 1 cut(s) 41
FaiI YATR 5 cut(s) 39, 251, 312, 327, 331
FaqI GGGAC 1 cut(s) 160
FatI CATG 1 cut(s) 37
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 7 cut(s) 80, 132, 135, 201, 216, 226, 366
FokI GGATG 3 cut(s) 86, 100, 112
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 7 cut(s) 80, 132, 135, 201, 216, 226, 366
FspBI CTAG 1 cut(s) 303
GluI GCNGC 7 cut(s) 80, 132, 135, 201, 216, 226, 366
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 10
Hin1II CATG 1 cut(s) 41
HpaII CCGG 1 cut(s) 10
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 3 cut(s) 17, 197, 242
Hpy188III TCNNGA 1 cut(s) 287
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 2 cut(s) 120, 163
HpyCH4III ACNGT 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 242
HpyF3I CTNAG 3 cut(s) 188, 194, 239
Hsp92II CATG 1 cut(s) 41
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 1 cut(s) 230
LmnI GCTCC 2 cut(s) 109, 142
LpnPI CCDG 5 cut(s) 23, 56, 81, 83, 175
Lsp1109I GCAGC 2 cut(s) 91, 202
MaeI CTAG 1 cut(s) 303
MalI GATC 1 cut(s) 232
MbiI CCGCTC 1 cut(s) 236
MboI GATC 1 cut(s) 230
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 1 cut(s) 18
MnlI CCTC 7 cut(s) 127, 151, 169, 188, 195, 214, 310
MseI TTAA 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 82, 228
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 71
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 3 cut(s) 131, 210, 228
MwoI GCNNNNNNNGC 2 cut(s) 131, 242
NdeII GATC 1 cut(s) 230
NlaIII CATG 1 cut(s) 41
NlaIV GGNNCC 3 cut(s) 105, 138, 232
PkrI GCNGC 7 cut(s) 81, 133, 136, 202, 217, 227, 367
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 3 cut(s) 105, 138, 232
PsuI RGATCY 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 82
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
SacI GAGCTC 1 cut(s) 247
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 7 cut(s) 80, 132, 135, 201, 216, 226, 366
Sau3AI GATC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 1 cut(s) 247
SetI ASST 6 cut(s) 72, 84, 112, 247, 302, 351
Sfr303I CCGCGG 1 cut(s) 229
SgrBI CCGCGG 1 cut(s) 229
Sse9I AATT 1 cut(s) 18
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 294
TasI AATT 1 cut(s) 18
TauI GCSGC 5 cut(s) 134, 137, 203, 228, 368
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 1 cut(s) 38
TseI GCWGC 2 cut(s) 79, 215
TspDTI ATGAA 2 cut(s) 102, 299
TspRI CASTG 1 cut(s) 38
XapI RAATTY 1 cut(s) 18
XspI CTAG 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.