Rmu_sc0003177.1_g000031

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003177.1
OG0008901
Physical Location & Seq
Forward (+)
124729 .. 126097
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003177.1_g000031.1.cds

Sequence Viewer

Length: 645 bp
atgcctttccggtttagaattttctccaaacagtgccatttggatttcgtcagcaccatttctatcaacctggatgagcagctgatgggttcgtcagggatggctccaccttcatcctctaaagcccacgcggcggctccctccactctcgccttctcctccgccgtctccaagtccctcaaccctctcaggctcagagccgcctctcgcggtgctgctattggccgcggatccgctctcagagctcgtatggtctcaatgccgacgattaagcccccgctctctcttgatttcgacacctctgttttcaacaaggagaagatcaacctcgctggtcacgatgagtatattgtaaagggaggaaggtacttgttccatctgctgcctgatgccttcaagggcatcaagcagatcggtgttatcggttggggttcacagggacctgctcaagctcagaacttaagagattctctagctgaagcaaagtcgaatattgtggtcaagattgggctcaggaagggatcctgttcatttaatgaagctcgcgcagctggtttcatcgaggagaatgggactttaggtgacatatgggaaacaatctcagagagtgatcttgtgttgctactaatttctgatcccgtatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

214

Amino Acids

22.97

Weight (kDa)

9.36

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
AccBSI CCGCTC 2 cut(s) 236, 280
AccII CGCG 4 cut(s) 131, 210, 228, 546
AciI CCGC 9 cut(s) 131, 134, 162, 201, 210, 226, 228, 234, 278
AclWI GGATC 5 cut(s) 225, 238, 516, 529, 629
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 133
AflII CTTAAG 1 cut(s) 460
AgsI TTSAA 2 cut(s) 310, 397
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 6 cut(s) 82, 245, 452, 476, 542, 551
AluI AGCT 6 cut(s) 82, 245, 452, 476, 542, 551
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 2 cut(s) 172, 259
AlwI GGATC 5 cut(s) 225, 238, 516, 529, 629
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 4 cut(s) 79, 215, 382, 548
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 593
AvaII GGWCC 1 cut(s) 440
BamHI GGATCC 2 cut(s) 230, 521
BanII GRGCYC 2 cut(s) 247, 513
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 4 cut(s) 91, 202, 369, 560
BccI CCATC 3 cut(s) 79, 94, 384
BceAI ACGGC 1 cut(s) 149
BciT130I CCWGG 1 cut(s) 71
BcoDI GTCTC 2 cut(s) 172, 259
BfaI CTAG 1 cut(s) 473
BfrI CTTAAG 1 cut(s) 460
BfuAI ACCTGC 1 cut(s) 451
BglI GCCNNNNNGGC 1 cut(s) 131
BisI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 383, 549
BlsI GCNGC 8 cut(s) 81, 133, 136, 202, 217, 227, 384, 550
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 5 cut(s) 105, 138, 232, 441, 523
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 379, 411
Bpu10I CCTNAGC 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 3 cut(s) 79, 105, 113
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 6 cut(s) 202, 208, 253, 467, 526, 615
BseRI GAGGAG 2 cut(s) 148, 578
BseXI GCAGC 4 cut(s) 91, 202, 369, 560
Bsh1236I CGCG 4 cut(s) 131, 210, 228, 546
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 3 cut(s) 160, 453, 586
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 2 cut(s) 172, 259
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 3 cut(s) 160, 453, 586
BsnI GGCC 1 cut(s) 225
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 247, 513
Bsp143I GATC 6 cut(s) 230, 321, 411, 521, 610, 634
BspACI CCGC 9 cut(s) 131, 134, 162, 201, 210, 226, 228, 234, 278
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 6 cut(s) 201, 207, 252, 466, 525, 614
BspFNI CGCG 4 cut(s) 131, 210, 228, 546
BspLI GGNNCC 5 cut(s) 105, 138, 232, 441, 523
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 5 cut(s) 225, 238, 516, 529, 629
BspTI CTTAAG 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 259
BsrBI CCGCTC 2 cut(s) 236, 280
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 6 cut(s) 230, 321, 411, 521, 610, 634
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 33
BstAFI CTTAAG 1 cut(s) 460
BstC8I GCNNGC 1 cut(s) 544
BstDEI CTNAG 6 cut(s) 188, 194, 239, 453, 512, 601
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 3 cut(s) 79, 105, 113
BstFNI CGCG 4 cut(s) 131, 210, 228, 546
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 6 cut(s) 233, 324, 414, 524, 613, 637
BstMAI GTCTC 2 cut(s) 172, 259
BstMBI GATC 6 cut(s) 230, 321, 411, 521, 610, 634
BstMWI GCNNNNNNNGC 3 cut(s) 131, 242, 548
BstNI CCWGG 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 69
BstUI CGCG 4 cut(s) 131, 210, 228, 546
BstV1I GCAGC 4 cut(s) 91, 202, 369, 560
BstX2I RGATCY 2 cut(s) 230, 521
BstYI RGATCY 2 cut(s) 230, 521
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 3 cut(s) 79, 105, 113
BtsIMutI CAGTG 1 cut(s) 38
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 1 cut(s) 544
CfoI GCGC 1 cut(s) 548
Cfr13I GGNCC 1 cut(s) 440
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 1 cut(s) 367
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 6 cut(s) 188, 194, 239, 453, 512, 601
DpnI GATC 6 cut(s) 232, 323, 413, 523, 612, 636
DpnII GATC 6 cut(s) 230, 321, 411, 521, 610, 634
EaeI YGGCCR 1 cut(s) 223
EciI GGCGGA 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 2 cut(s) 247, 513
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 440
Eco53kI GAGCTC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 498
EcoICRI GAGCTC 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 69
EcoT38I GRGCYC 2 cut(s) 247, 513
Esp3I CGTCTC 1 cut(s) 172
FaiI YATR 5 cut(s) 251, 348, 587, 589, 643
FaqI GGGAC 3 cut(s) 160, 453, 586
FauI CCCGC 1 cut(s) 285
FauNDI CATATG 1 cut(s) 587
Fnu4HI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 383, 549
FokI GGATG 3 cut(s) 86, 100, 112
FriOI GRGCYC 2 cut(s) 247, 513
Fsp4HI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 383, 549
FspBI CTAG 1 cut(s) 473
GlaI GCGC 1 cut(s) 547
GluI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 383, 549
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 10
HhaI GCGC 1 cut(s) 548
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 10
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 5 cut(s) 197, 242, 456, 604, 634
Hpy188III TCNNGA 4 cut(s) 287, 338, 502, 514
Hpy8I GTNNAC 1 cut(s) 434
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 5 cut(s) 120, 163, 357, 403, 511
HpyCH4III ACNGT 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 242, 548
HpyF3I CTNAG 6 cut(s) 188, 194, 239, 453, 512, 601
HspAI GCGC 1 cut(s) 546
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 6 cut(s) 230, 321, 411, 521, 610, 634
LmnI GCTCC 2 cut(s) 109, 142
Lsp1109I GCAGC 4 cut(s) 91, 202, 369, 560
LweI GCATC 2 cut(s) 379, 411
MaeI CTAG 1 cut(s) 473
MaeIII GTNAC 2 cut(s) 335, 581
MalI GATC 6 cut(s) 232, 323, 413, 523, 612, 636
MbiI CCGCTC 2 cut(s) 236, 280
MboI GATC 6 cut(s) 230, 321, 411, 521, 610, 634
MboII GAAGA 1 cut(s) 331
MflI RGATCY 2 cut(s) 230, 521
MhlI GDGCHC 2 cut(s) 247, 513
MluCI AATT 2 cut(s) 18, 627
MseI TTAA 3 cut(s) 270, 461, 534
MspA1I CMGCKG 3 cut(s) 82, 228, 551
MspCI CTTAAG 1 cut(s) 460
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 71
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 4 cut(s) 131, 210, 228, 546
MwoI GCNNNNNNNGC 3 cut(s) 131, 242, 548
NdeI CATATG 1 cut(s) 587
NdeII GATC 6 cut(s) 230, 321, 411, 521, 610, 634
NlaIV GGNNCC 5 cut(s) 105, 138, 232, 441, 523
NmuCI GTSAC 2 cut(s) 335, 581
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 8 cut(s) 81, 133, 136, 202, 217, 227, 384, 550
PpuMI RGGWCCY 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 5 cut(s) 105, 138, 232, 441, 523
PspPI GGNCC 1 cut(s) 440
PspPPI RGGWCCY 1 cut(s) 440
PsuI RGATCY 2 cut(s) 230, 521
PvuII CAGCTG 2 cut(s) 82, 551
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SacI GAGCTC 1 cut(s) 247
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 3 cut(s) 270, 461, 534
SatI GCNGC 8 cut(s) 80, 132, 135, 201, 216, 226, 383, 549
Sau3AI GATC 6 cut(s) 230, 321, 411, 521, 610, 634
Sau96I GGNCC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 2 cut(s) 247, 513
SfaNI GCATC 2 cut(s) 379, 411
Sfr303I CCGCGG 1 cut(s) 229
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 447, 460
SmoI CTYRAG 2 cut(s) 447, 460
Sse9I AATT 2 cut(s) 18, 627
SsiI CCGC 9 cut(s) 131, 134, 162, 201, 210, 226, 228, 234, 278
SspI AATATT 1 cut(s) 493
SspMI CTAG 1 cut(s) 473
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 294, 488, 561
TasI AATT 2 cut(s) 18, 627
TauI GCSGC 4 cut(s) 134, 137, 203, 228
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 270, 461, 534
Tru9I TTAA 3 cut(s) 270, 461, 534
TscAI CASTG 1 cut(s) 38
TseFI GTSAC 2 cut(s) 335, 581
TseI GCWGC 4 cut(s) 79, 215, 382, 548
Tsp45I GTSAC 2 cut(s) 335, 581
TspDTI ATGAA 4 cut(s) 102, 519, 547, 552
TspRI CASTG 1 cut(s) 38
Vha464I CTTAAG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 18
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.