Rh1DG008100

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0008901
Physical Location & Seq
Reverse (-)
1104986 .. 1108936
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG008100.1

Sequence Viewer

Length: 1119 bp
ATGCCTTTCCGGTTCAGAATTTTCTCCAAACAGTGCCATGTGGATTTCGTCATCACCATTTCTATCAACCTGGATGAGCAGATGATGGGTTCGTCAGGGATGGCTCCACCTTCATCCTCTAAAGCCCACGCGGCGGCTCCCTCCACTGTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCATAGCCGCCTCTCGCGGTGCTGCTATTGGCCGAGGATCCGCTCTCAGAGCTCGTACGGTCTCAATGCTGACGATTAAGCCCCCGCTCTCTCTTGATTTCGACACCTCTGTTTTCAACAAGGAGAAGATCAACCTCGCTGGTCACGATGAGTATATTGTAAAGGGAGGAAGGTACTTGTTCCATCTGCTGACTGATGCCTTCAAGGGCATCAAGCAGATCGGTGTTATCGGTTGGGGTTCACAGGGACCTGCTCAAGCTCAGAACTTAAGAGATTCTCTAGCTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCTGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACCGAGGAGAATGGGACTTTAGGTGACATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCCGCACAGGCAGATAATTATGAGAAAATATTTTCGCACATGAAACCAAATAGCATATTTGGTCTTTCCCACGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTGTTCAGAAGATACACCGACAGCAGAATGAGTGAAGATTCTGCTTATAAAAATACTATTGAATGTGTTACTGGAATTGTGTCAAGAACCATATCAACTAAGGGCATGTTGGAAATATACAGCTCTTTGTCTGAAGCTGACAAGAAGGAGTTTGAGACTGCATATAGTGCCTCCTATTATCCTTGCATGGACATCCTCTATGAATGCTATGAGGATGTAGCAAGCGGCAGTGAGATCCGTAGCATTGTTTTGGCTGAGCAGCGATTTTATGAGAAAGATGGTCTTCCTGCCTTCCCTATGGGAAAGATTGATCAGACGTGCATGTGGAAAGTTGGTGAGCGTGTCCAATCGACAGAATGTAGTGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

372

Amino Acids

40.67

Weight (kDa)

6.21

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 136 - 238 1.9e-15 Acetohydroxy acid isomeroreductase, NADPH-binding domain
KARI_C PF01450 236 - 366 4.9e-22 Ketol-acid reductoisomerase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
Acc36I ACCTGC 1 cut(s) 451
AccBSI CCGCTC 2 cut(s) 236, 280
AccII CGCG 3 cut(s) 131, 210, 546
AciI CCGC 9 cut(s) 131, 134, 162, 201, 210, 234, 278, 639, 972
AclWI GGATC 6 cut(s) 225, 238, 516, 529, 629, 976
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 2 cut(s) 498, 900
AfaI GTAC 2 cut(s) 250, 368
AfiI CCNNNNNNNGG 1 cut(s) 133
AflII CTTAAG 1 cut(s) 460
AgsI TTSAA 3 cut(s) 310, 397, 809
AjiI CACGTC 1 cut(s) 1065
AjnI CCWGG 1 cut(s) 69
AloI GAACNNNNNNTCC 2 cut(s) 734, 766
AluBI AGCT 7 cut(s) 245, 452, 476, 542, 551, 870, 884
AluI AGCT 7 cut(s) 245, 452, 476, 542, 551, 870, 884
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 3 cut(s) 172, 259, 896
AlwI GGATC 6 cut(s) 225, 238, 516, 529, 629, 976
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 3 cut(s) 215, 548, 1006
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 4 cut(s) 46, 550, 593, 1094
AvaII GGWCC 1 cut(s) 440
BamHI GGATCC 2 cut(s) 230, 521
BanII GRGCYC 2 cut(s) 247, 513
BbsI GAAGAC 1 cut(s) 1022
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 202, 560, 1018
BccI CCATC 4 cut(s) 79, 94, 384, 1019
BceAI ACGGC 2 cut(s) 149, 724
BciT130I CCWGG 1 cut(s) 71
BclI TGATCA 1 cut(s) 1057
BcoDI GTCTC 3 cut(s) 172, 259, 896
BfaI CTAG 1 cut(s) 473
BfrI CTTAAG 1 cut(s) 460
BfuAI ACCTGC 1 cut(s) 451
BglI GCCNNNNNGGC 1 cut(s) 131
BisI GCNGC 7 cut(s) 132, 135, 201, 216, 549, 973, 1007
BlpI GCTNAGC 1 cut(s) 1002
BlsI GCNGC 7 cut(s) 133, 136, 202, 217, 550, 974, 1008
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 440
BmgBI CACGTC 1 cut(s) 1065
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 5 cut(s) 105, 138, 232, 441, 523
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 379, 411
BpiI GAAGAC 1 cut(s) 1022
Bpu10I CCTNAGC 1 cut(s) 512
Bpu1102I GCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 3 cut(s) 226, 561, 706
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 823
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 226, 561, 706
BseGI GGATG 5 cut(s) 79, 105, 113, 939, 967
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 6 cut(s) 202, 253, 467, 526, 615, 993
BseNI ACTGG 1 cut(s) 823
BseRI GAGGAG 2 cut(s) 148, 578
BseXI GCAGC 3 cut(s) 202, 560, 1018
Bsh1236I CGCG 3 cut(s) 131, 210, 546
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 10
BsiWI CGTACG 1 cut(s) 248
BslFI GGGAC 4 cut(s) 160, 453, 586, 730
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 3 cut(s) 172, 259, 896
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 4 cut(s) 160, 453, 586, 730
BsmI GAATGC 1 cut(s) 956
BsnI GGCC 1 cut(s) 225
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 247, 513
Bsp143I GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
Bsp1720I GCTNAGC 1 cut(s) 1002
BspACI CCGC 9 cut(s) 131, 134, 162, 201, 210, 234, 278, 639, 972
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 6 cut(s) 201, 252, 466, 525, 614, 994
BspFNI CGCG 3 cut(s) 131, 210, 546
BspLI GGNNCC 5 cut(s) 105, 138, 232, 441, 523
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 6 cut(s) 225, 238, 516, 529, 629, 976
BspTI CTTAAG 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 259
BsrBI CCGCTC 2 cut(s) 236, 280
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 3 cut(s) 226, 561, 706
BssMI GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 3 cut(s) 33, 148, 253
BstAFI CTTAAG 1 cut(s) 460
BstAPI GCANNNNNTGC 1 cut(s) 914
BstC8I GCNNGC 2 cut(s) 544, 970
BstDEI CTNAG 7 cut(s) 188, 239, 453, 512, 601, 846, 1002
BstDSI CCRYGG 1 cut(s) 706
BstF5I GGATG 5 cut(s) 79, 105, 113, 939, 967
BstFNI CGCG 3 cut(s) 131, 210, 546
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 8 cut(s) 233, 324, 414, 524, 613, 637, 984, 1060
BstMAI GTCTC 3 cut(s) 172, 259, 896
BstMBI GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
BstMWI GCNNNNNNNGC 4 cut(s) 131, 242, 548, 914
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 2 cut(s) 856, 1072
BstSCI CCNGG 1 cut(s) 69
BstUI CGCG 3 cut(s) 131, 210, 546
BstV1I GCAGC 3 cut(s) 202, 560, 1018
BstV2I GAAGAC 1 cut(s) 1022
BstX2I RGATCY 3 cut(s) 230, 521, 981
BstYI RGATCY 3 cut(s) 230, 521, 981
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 706
BtrI CACGTC 1 cut(s) 1065
BtsCI GGATG 5 cut(s) 79, 105, 113, 939, 967
BtsI GCAGTG 1 cut(s) 982
BtsIMutI CAGTG 3 cut(s) 38, 144, 982
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 2 cut(s) 544, 970
CfoI GCGC 1 cut(s) 548
Cfr13I GGNCC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 249, 367
CviAII CATG 6 cut(s) 38, 676, 731, 853, 934, 1069
CviQI GTAC 2 cut(s) 249, 367
DdeI CTNAG 7 cut(s) 188, 239, 453, 512, 601, 846, 1002
DpnI GATC 8 cut(s) 232, 323, 413, 523, 612, 636, 983, 1059
DpnII GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
EaeI YGGCCR 1 cut(s) 223
EciI GGCGGA 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 245
Eco24I GRGCYC 2 cut(s) 247, 513
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 440
Eco53kI GAGCTC 1 cut(s) 245
Eco57I CTGAAG 2 cut(s) 498, 900
EcoICRI GAGCTC 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 69
EcoT38I GRGCYC 2 cut(s) 247, 513
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 6 cut(s) 41, 679, 734, 856, 937, 1072
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 4 cut(s) 160, 453, 586, 730
FatI CATG 6 cut(s) 37, 675, 730, 852, 933, 1068
FauI CCCGC 2 cut(s) 285, 646
FauNDI CATATG 1 cut(s) 587
FbaI TGATCA 1 cut(s) 1057
Fnu4HI GCNGC 7 cut(s) 132, 135, 201, 216, 549, 973, 1007
FokI GGATG 5 cut(s) 86, 100, 112, 926, 974
FriOI GRGCYC 2 cut(s) 247, 513
Fsp4HI GCNGC 7 cut(s) 132, 135, 201, 216, 549, 973, 1007
FspBI CTAG 1 cut(s) 473
GlaI GCGC 1 cut(s) 547
GluI GCNGC 7 cut(s) 132, 135, 201, 216, 549, 973, 1007
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 10
HhaI GCGC 1 cut(s) 548
Hin1II CATG 6 cut(s) 41, 679, 734, 856, 937, 1072
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HinfI GANTC 3 cut(s) 467, 743, 785
HpaII CCGG 1 cut(s) 10
HphI GGTGA 4 cut(s) 46, 550, 593, 1094
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 8 cut(s) 17, 242, 456, 604, 634, 755, 880, 1062
Hpy188III TCNNGA 5 cut(s) 287, 338, 502, 514, 831
Hpy8I GTNNAC 1 cut(s) 434
HpyAV CCTTC 7 cut(s) 120, 163, 357, 403, 511, 886, 1048
HpyCH4III ACNGT 3 cut(s) 33, 148, 253
HpyCH4IV ACGT 1 cut(s) 1064
HpyCH4V TGCA 3 cut(s) 908, 933, 1068
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 242, 548, 914
HpyF3I CTNAG 7 cut(s) 188, 239, 453, 512, 601, 846, 1002
HpySE526I ACGT 1 cut(s) 1064
Hsp92II CATG 6 cut(s) 41, 679, 734, 856, 937, 1072
HspAI GCGC 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 1057
Kzo9I GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
LmnI GCTCC 2 cut(s) 109, 142
Lsp1109I GCAGC 3 cut(s) 202, 560, 1018
LweI GCATC 2 cut(s) 379, 411
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 1064
MaeIII GTNAC 3 cut(s) 335, 581, 814
MalI GATC 8 cut(s) 232, 323, 413, 523, 612, 636, 983, 1059
MbiI CCGCTC 2 cut(s) 236, 280
MboI GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
MboII GAAGA 4 cut(s) 331, 768, 794, 1022
MflI RGATCY 3 cut(s) 230, 521, 981
MhlI GDGCHC 2 cut(s) 247, 513
MluCI AATT 5 cut(s) 18, 627, 652, 735, 822
MlyI GAGTC 1 cut(s) 752
MmeI TCCRAC 1 cut(s) 837
MseI TTAA 3 cut(s) 270, 461, 534
MspA1I CMGCKG 1 cut(s) 551
MspCI CTTAAG 1 cut(s) 460
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 71
Mva1269I GAATGC 1 cut(s) 956
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 3 cut(s) 131, 210, 546
MwoI GCNNNNNNNGC 4 cut(s) 131, 242, 548, 914
NdeI CATATG 1 cut(s) 587
NdeII GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
NlaIII CATG 6 cut(s) 41, 679, 734, 856, 937, 1072
NlaIV GGNNCC 5 cut(s) 105, 138, 232, 441, 523
NmeAIII GCCGAG 1 cut(s) 251
NmuCI GTSAC 2 cut(s) 335, 581
NspI RCATGY 2 cut(s) 856, 1072
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PctI GAATGC 1 cut(s) 956
PfeI GAWTC 2 cut(s) 467, 785
Pfl23II CGTACG 1 cut(s) 248
PkrI GCNGC 7 cut(s) 133, 136, 202, 217, 550, 974, 1008
PleI GAGTC 1 cut(s) 751
PpsI GAGTC 1 cut(s) 751
PpuMI RGGWCCY 1 cut(s) 440
PsiI TTATAA 1 cut(s) 795
Psp124BI GAGCTC 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspLI CGTACG 1 cut(s) 248
PspN4I GGNNCC 5 cut(s) 105, 138, 232, 441, 523
PspPI GGNCC 1 cut(s) 440
PspPPI RGGWCCY 1 cut(s) 440
PsuI RGATCY 3 cut(s) 230, 521, 981
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 250, 368
RsaNI GTAC 2 cut(s) 249, 367
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 3 cut(s) 270, 461, 534
SatI GCNGC 7 cut(s) 132, 135, 201, 216, 549, 973, 1007
Sau3AI GATC 8 cut(s) 230, 321, 411, 521, 610, 634, 981, 1057
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 1 cut(s) 752
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 2 cut(s) 247, 513
SfaNI GCATC 2 cut(s) 379, 411
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 447, 460
SmoI CTYRAG 2 cut(s) 447, 460
Sse9I AATT 5 cut(s) 18, 627, 652, 735, 822
SsiI CCGC 9 cut(s) 131, 134, 162, 201, 210, 234, 278, 639, 972
SspI AATATT 2 cut(s) 493, 666
SspMI CTAG 1 cut(s) 473
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 69
TaaI ACNGT 3 cut(s) 33, 148, 253
TaiI ACGT 1 cut(s) 1067
TaqI TCGA 3 cut(s) 294, 488, 1097
TasI AATT 5 cut(s) 18, 627, 652, 735, 822
TauI GCSGC 4 cut(s) 134, 137, 203, 975
TfiI GAWTC 2 cut(s) 467, 785
Tru1I TTAA 3 cut(s) 270, 461, 534
Tru9I TTAA 3 cut(s) 270, 461, 534
TscAI CASTG 3 cut(s) 38, 151, 982
TseFI GTSAC 2 cut(s) 335, 581
TseI GCWGC 3 cut(s) 215, 548, 1006
Tsp45I GTSAC 2 cut(s) 335, 581
TspDTI ATGAA 6 cut(s) 102, 519, 552, 692, 719, 963
TspGWI ACGGA 1 cut(s) 974
TspRI CASTG 3 cut(s) 38, 151, 982
Vha464I CTTAAG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 18
XceI RCATGY 2 cut(s) 856, 1072
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.