Rh5CG571600

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008901
Physical Location & Seq
Forward (+)
80814519 .. 80815802
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG571600.1

Sequence Viewer

Length: 597 bp
ATGGCTCCACCTTCATCCTCTAAAGCCCACGCGGCGGCTCCCTCCACTCTCGCCTTCTCCTCCGCCGTCTCCAAGTCCCTCAACCCTCTCAGGCTCAGAGCCGCCTCTCGCGGTGCTGCTATTGGCCGCGGATCCGCTCTCAGAGCTCGTATGGTCTCAATGCCGACGATTAAGCCCCCGCTCTCTCGTGATTTCGACACCTCTGTTTTCAACAAGGAGAATATCAACCTCGCTGGTCACGATGAGAGTATTCATAGTGATTTGAGATATGGTATTTGGTTTTTGTTGATTTTGCAGTATATTGTAAAGGGAGAAAGGTACTTGTTCCATCTGCCGCCTGATGCCTTCAACTCTTCAAGGACATCAAGCATATCGGTGTTATCGGTTGGGGGACCTGCTCAAGCTCAGAACTTAAGAGATTCTCTAGCTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCATCGAGGAGAATGGGACTTTAGGTGACATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

198

Amino Acids

21.3

Weight (kDa)

9.56

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 403
AccBSI CCGCTC 2 cut(s) 137, 181
AccII CGCG 4 cut(s) 32, 111, 129, 498
AclWI GGATC 5 cut(s) 126, 139, 468, 481, 581
AcoI YGGCCR 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 450
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 34
AflII CTTAAG 1 cut(s) 412
AgsI TTSAA 3 cut(s) 211, 349, 357
AluBI AGCT 5 cut(s) 146, 404, 428, 494, 503
AluI AGCT 5 cut(s) 146, 404, 428, 494, 503
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 2 cut(s) 73, 160
AlwI GGATC 5 cut(s) 126, 139, 468, 481, 581
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 2 cut(s) 116, 500
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 1 cut(s) 545
AvaII GGWCC 1 cut(s) 392
BamHI GGATCC 2 cut(s) 131, 473
BanII GRGCYC 2 cut(s) 148, 465
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 2 cut(s) 103, 512
BccI CCATC 1 cut(s) 336
BceAI ACGGC 1 cut(s) 50
BcoDI GTCTC 2 cut(s) 73, 160
BfaI CTAG 1 cut(s) 425
BfrI CTTAAG 1 cut(s) 412
BfuAI ACCTGC 1 cut(s) 403
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 7 cut(s) 33, 36, 102, 117, 127, 335, 501
BlsI GCNGC 7 cut(s) 34, 37, 103, 118, 128, 336, 502
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 5 cut(s) 6, 39, 133, 393, 475
BmsI GCATC 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 1 cut(s) 127
Bsc4I CCNNNNNNNGG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 6 cut(s) 103, 109, 154, 419, 478, 567
BseRI GAGGAG 2 cut(s) 49, 530
BseXI GCAGC 2 cut(s) 103, 512
Bsh1236I CGCG 4 cut(s) 32, 111, 129, 498
BshFI GGCC 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 148
BslFI GGGAC 3 cut(s) 61, 405, 538
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 73, 160
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 3 cut(s) 61, 405, 538
BsnI GGCC 1 cut(s) 126
Bso31I GGTCTC 1 cut(s) 160
Bsp1286I GDGCHC 2 cut(s) 148, 465
Bsp143I GATC 4 cut(s) 131, 473, 562, 586
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 6 cut(s) 102, 108, 153, 418, 477, 566
BspFNI CGCG 4 cut(s) 32, 111, 129, 498
BspLI GGNNCC 5 cut(s) 6, 39, 133, 393, 475
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 5 cut(s) 126, 139, 468, 481, 581
BspTI CTTAAG 1 cut(s) 412
BspTNI GGTCTC 1 cut(s) 160
BsrBI CCGCTC 2 cut(s) 137, 181
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 4 cut(s) 131, 473, 562, 586
BssSI CACGAG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 358
BstAFI CTTAAG 1 cut(s) 412
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 6 cut(s) 89, 95, 140, 405, 464, 553
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 4 cut(s) 32, 111, 129, 498
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 4 cut(s) 134, 476, 565, 589
BstMAI GTCTC 2 cut(s) 73, 160
BstMBI GATC 4 cut(s) 131, 473, 562, 586
BstMWI GCNNNNNNNGC 3 cut(s) 32, 143, 500
BstUI CGCG 4 cut(s) 32, 111, 129, 498
BstV1I GCAGC 2 cut(s) 103, 512
BstX2I RGATCY 2 cut(s) 131, 473
BstYI RGATCY 2 cut(s) 131, 473
BsuRI GGCC 1 cut(s) 126
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 1 cut(s) 14
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 496
CfoI GCGC 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 392
Cfr42I CCGCGG 1 cut(s) 130
Csp6I GTAC 1 cut(s) 319
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 6 cut(s) 89, 95, 140, 405, 464, 553
DpnI GATC 4 cut(s) 133, 475, 564, 588
DpnII GATC 4 cut(s) 131, 473, 562, 586
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 146
Eco24I GRGCYC 2 cut(s) 148, 465
Eco31I GGTCTC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 450
EcoICRI GAGCTC 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 392
EcoT38I GRGCYC 2 cut(s) 148, 465
Esp3I CGTCTC 1 cut(s) 73
FaiI YATR 8 cut(s) 152, 255, 270, 300, 371, 539, 541, 595
FaqI GGGAC 3 cut(s) 61, 405, 538
FauI CCCGC 1 cut(s) 186
FauNDI CATATG 1 cut(s) 539
Fnu4HI GCNGC 7 cut(s) 33, 36, 102, 117, 127, 335, 501
FriOI GRGCYC 2 cut(s) 148, 465
Fsp4HI GCNGC 7 cut(s) 33, 36, 102, 117, 127, 335, 501
FspBI CTAG 1 cut(s) 425
GlaI GCGC 1 cut(s) 499
GluI GCNGC 7 cut(s) 33, 36, 102, 117, 127, 335, 501
HaeIII GGCC 1 cut(s) 126
HhaI GCGC 1 cut(s) 500
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HinfI GANTC 1 cut(s) 419
HphI GGTGA 1 cut(s) 545
Hpy188I TCNGA 5 cut(s) 98, 143, 408, 556, 586
Hpy188III TCNNGA 4 cut(s) 188, 239, 454, 466
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 4 cut(s) 21, 64, 355, 463
HpyCH4V TGCA 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 143, 500
HpyF3I CTNAG 6 cut(s) 89, 95, 140, 405, 464, 553
HspAI GCGC 1 cut(s) 498
KspI CCGCGG 1 cut(s) 130
Kzo9I GATC 4 cut(s) 131, 473, 562, 586
LmnI GCTCC 2 cut(s) 10, 43
LpnPI CCDG 6 cut(s) 76, 219, 351, 408, 451, 489
Lsp1109I GCAGC 2 cut(s) 103, 512
LweI GCATC 1 cut(s) 331
MaeI CTAG 1 cut(s) 425
MaeIII GTNAC 2 cut(s) 236, 533
MalI GATC 4 cut(s) 133, 475, 564, 588
MbiI CCGCTC 2 cut(s) 137, 181
MboI GATC 4 cut(s) 131, 473, 562, 586
MboII GAAGA 1 cut(s) 345
MflI RGATCY 2 cut(s) 131, 473
MhlI GDGCHC 2 cut(s) 148, 465
MluCI AATT 1 cut(s) 579
MnlI CCTC 9 cut(s) 28, 52, 70, 89, 96, 115, 211, 239, 508
MseI TTAA 3 cut(s) 171, 413, 486
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 2 cut(s) 129, 503
MspCI CTTAAG 1 cut(s) 412
MvnI CGCG 4 cut(s) 32, 111, 129, 498
MwoI GCNNNNNNNGC 3 cut(s) 32, 143, 500
NdeI CATATG 1 cut(s) 539
NdeII GATC 4 cut(s) 131, 473, 562, 586
NlaIV GGNNCC 5 cut(s) 6, 39, 133, 393, 475
NmuCI GTSAC 2 cut(s) 236, 533
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 7 cut(s) 34, 37, 103, 118, 128, 336, 502
PpuMI RGGWCCY 1 cut(s) 392
Psp124BI GAGCTC 1 cut(s) 148
Psp5II RGGWCCY 1 cut(s) 392
PspN4I GGNNCC 5 cut(s) 6, 39, 133, 393, 475
PspPI GGNCC 1 cut(s) 392
PspPPI RGGWCCY 1 cut(s) 392
PsuI RGATCY 2 cut(s) 131, 473
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 148
SacII CCGCGG 1 cut(s) 130
SaqAI TTAA 3 cut(s) 171, 413, 486
SatI GCNGC 7 cut(s) 33, 36, 102, 117, 127, 335, 501
Sau3AI GATC 4 cut(s) 131, 473, 562, 586
Sau96I GGNCC 1 cut(s) 392
SduI GDGCHC 2 cut(s) 148, 465
SfaNI GCATC 1 cut(s) 331
Sfr303I CCGCGG 1 cut(s) 130
SgrBI CCGCGG 1 cut(s) 130
SinI GGWCC 1 cut(s) 392
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 2 cut(s) 399, 412
SmoI CTYRAG 2 cut(s) 399, 412
Sse9I AATT 1 cut(s) 579
SspI AATATT 1 cut(s) 445
SspMI CTAG 1 cut(s) 425
SstI GAGCTC 1 cut(s) 148
TaqI TCGA 3 cut(s) 195, 440, 513
TasI AATT 1 cut(s) 579
TauI GCSGC 5 cut(s) 35, 38, 104, 129, 337
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 3 cut(s) 171, 413, 486
Tru9I TTAA 3 cut(s) 171, 413, 486
TseFI GTSAC 2 cut(s) 236, 533
TseI GCWGC 2 cut(s) 116, 500
Tsp45I GTSAC 2 cut(s) 236, 533
TspDTI ATGAA 4 cut(s) 242, 471, 499, 504
Vha464I CTTAAG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 392
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.