RchiOBHm_Chr5g0013651

serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007555
Physical Location & Seq
Forward (+)
9194140 .. 9198466
4327 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29416

Sequence Viewer

Length: 903 bp
ATGACAGTGGTTTGCGAGCGTCATAATCGTTCAGTTGCTGATCTTTGTTATGGTCCAGTGACTCCAGATGGGGTCTTGCTCCTCACTGCTAGTGAAGATCACACTGCCATCTTACGAAATGGAGAGACAGGAGGATTCATCCATAAATTTGAAGGACATGAAGGACAAGTGTGGAGTTGCGATATAAATTCAATGGGACGACTGGCTGCAACCGCCTCGGAAGATTGTACAGTGAGAATTTGGGATATAAATGCTAGACAGTGTGTACATAAATTTTCAGAGGGCAATATAATGCAGTCATGTGCTTTTTCACTGGTTGGCAACTATATTGTTATGGGGGGATTTAGAGATAATCTCAAATTGTACGACTTGCATCAAGTTGATACTCCGATATGGCAAATAGCAAACCTTGATGCATGTGTTAGATCTGTGGCATGGATGGACGACCATAGACTAATGGTTTCTTTTAGTGATAAAGTGGGTTTTAGGGTATGGGATATAAGAGGTCCTCCTTGCATAAAAGAGATTCGGACTCATTCATCTGTGACAAGCATTGAACTGCACCAAAGTAAGAATTACATCACAACGATGAGTGCAAACACCGCTACTATTTGGAACCTTACATTTAACCCTCTGGTGGAGCTATGTGTGCCACGCATATCAGAGTCAGCCTCTTTCAGACCACAAAATGATATTTTGATTGTTGGAGGAGATGATAGAGTGGTTCGTATACTGGGTGTCAAAGATCGTACTGAGATTAGTGTTGTAGGCGGCGGGTTTCACATGGGTCCCATTCAGTGTGTTCGCTTCTCTCCGAATGGCCAATCATTTGCAGCAGGATCCCGAGACGGAAGAACGATAATTCGGAAAATTGATATACAAATGGAAGATGTGAATGATTGA

Protein Analysis

300

Amino Acids

33.39

Weight (kDa)

5.92

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 3 - 124 2.7e-13 WDR3 first beta-propeller domain
WDR55 PF24796 5 - 95 3.2e-06 WDR55
EIF3I PF24805 6 - 294 1.8e-27 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 7 - 97 1.1e-06 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 18 - 157 5.9e-15 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 20 - 93 2.6e-10 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 22 - 208 2.2e-25 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 25 - 94 4.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 25 - 112 6.2e-10 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 27 - 166 1.6e-09 G protein beta WD-40 repeat protein
WD40 PF00400 46 - 82 3.9e-10 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 57 - 205 1.1e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 68 - 209 2.1e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 100 - 205 2.8e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 100 - 208 5.8e-09 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 102 - 206 3.1e-06 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 107 - 284 6e-17 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 175 - 291 1.2e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 187 - 288 8.6e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 223 - 287 9.1e-06 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 225 - 285 6.6e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 730
AciI CCGC 4 cut(s) 213, 603, 771, 774
AclWI GGATC 2 cut(s) 834, 847
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 4 cut(s) 146, 187, 237, 272
AfaI GTAC 4 cut(s) 229, 267, 365, 751
AfiI CCNNNNNNNGG 1 cut(s) 637
AgsI TTSAA 3 cut(s) 152, 192, 557
AluBI AGCT 1 cut(s) 643
AluI AGCT 1 cut(s) 643
Alw26I GTCTC 2 cut(s) 119, 840
AlwI GGATC 2 cut(s) 834, 847
AlwNI CAGNNNCTG 1 cut(s) 38
Ama87I CYCGRG 1 cut(s) 843
AoxI GGCC 1 cut(s) 820
ApeKI GCWGC 2 cut(s) 206, 833
ApoI RAATTY 4 cut(s) 146, 187, 237, 272
AspS9I GGNCC 3 cut(s) 53, 506, 788
AvaI CYCGRG 1 cut(s) 843
AvaII GGWCC 3 cut(s) 53, 506, 788
BalI TGGCCA 1 cut(s) 822
BamHI GGATCC 1 cut(s) 839
BbvI GCAGC 2 cut(s) 193, 845
BccI CCATC 3 cut(s) 62, 116, 433
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BcoDI GTCTC 2 cut(s) 119, 840
BfaI CTAG 2 cut(s) 90, 255
BglII AGATCT 1 cut(s) 425
BisI GCNGC 3 cut(s) 207, 772, 834
BlsI GCNGC 3 cut(s) 208, 773, 835
Bme18I GGWCC 3 cut(s) 53, 506, 788
BmeT110I CYCGRG 1 cut(s) 843
BmgT120I GGNCC 3 cut(s) 53, 506, 788
BmiI GGNNCC 4 cut(s) 617, 789, 790, 841
BmrI ACTGGG 1 cut(s) 743
BmsI GCATC 2 cut(s) 382, 403
BmuI ACTGGG 1 cut(s) 743
BplI GAGNNNNNCTC 4 cut(s) 339, 371, 656, 688
BpmI CTGGAG 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 637
Bse1I ACTGG 4 cut(s) 56, 207, 318, 738
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 2 cut(s) 138, 444
BseLI CCNNNNNNNGG 1 cut(s) 637
BseMII CTCAG 1 cut(s) 744
BseNI ACTGG 4 cut(s) 56, 207, 318, 738
BseRI GAGGAG 2 cut(s) 71, 723
BseXI GCAGC 2 cut(s) 193, 845
BsgI GTGCAG 1 cut(s) 545
BshFI GGCC 1 cut(s) 822
BsiHKCI CYCGRG 1 cut(s) 843
BslFI GGGAC 2 cut(s) 210, 774
BslI CCNNNNNNNGG 1 cut(s) 637
BsmAI GTCTC 2 cut(s) 119, 840
BsmBI CGTCTC 1 cut(s) 840
BsmFI GGGAC 2 cut(s) 210, 774
BsnI GGCC 1 cut(s) 822
BsoBI CYCGRG 1 cut(s) 843
Bsp1407I TGTACA 2 cut(s) 227, 265
Bsp143I GATC 5 cut(s) 40, 97, 425, 745, 839
BspACI CCGC 4 cut(s) 213, 603, 771, 774
BspANI GGCC 1 cut(s) 822
BspCNI CTCAG 1 cut(s) 745
BspLI GGNNCC 4 cut(s) 617, 789, 790, 841
BspPI GGATC 2 cut(s) 834, 847
BsrGI TGTACA 2 cut(s) 227, 265
BsrI ACTGG 4 cut(s) 56, 207, 318, 738
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 5 cut(s) 40, 97, 425, 745, 839
BssNAI GTATAC 1 cut(s) 731
Bst1107I GTATAC 1 cut(s) 731
Bst4CI ACNGT 3 cut(s) 7, 232, 261
BstAUI TGTACA 2 cut(s) 227, 265
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 753
BstF5I GGATG 2 cut(s) 138, 444
BstKTI GATC 5 cut(s) 43, 100, 428, 748, 842
BstMAI GTCTC 2 cut(s) 119, 840
BstMBI GATC 5 cut(s) 40, 97, 425, 745, 839
BstMWI GCNNNNNNNGC 3 cut(s) 212, 602, 649
BstNSI RCATGY 1 cut(s) 420
BstV1I GCAGC 2 cut(s) 193, 845
BstX2I RGATCY 2 cut(s) 425, 839
BstYI RGATCY 2 cut(s) 425, 839
BstZ17I GTATAC 1 cut(s) 731
BsuRI GGCC 1 cut(s) 822
BtsCI GGATG 2 cut(s) 138, 444
BtsI GCAGTG 2 cut(s) 84, 102
BtsIMutI CAGTG 8 cut(s) 12, 63, 84, 102, 237, 266, 311, 803
Cac8I GCNNGC 1 cut(s) 17
CaiI CAGNNNCTG 1 cut(s) 38
Cfr13I GGNCC 3 cut(s) 53, 506, 788
CseI GACGC 1 cut(s) 8
Csp6I GTAC 4 cut(s) 228, 266, 364, 750
CviAII CATG 5 cut(s) 158, 300, 417, 435, 784
CviJI RGCY 4 cut(s) 206, 643, 671, 822
CviKI_1 RGCY 4 cut(s) 206, 643, 671, 822
CviQI GTAC 4 cut(s) 228, 266, 364, 750
DdeI CTNAG 1 cut(s) 753
DpnI GATC 5 cut(s) 42, 99, 427, 747, 841
DpnII GATC 5 cut(s) 40, 97, 425, 745, 839
EaeI YGGCCR 1 cut(s) 820
Eco47I GGWCC 3 cut(s) 53, 506, 788
Eco88I CYCGRG 1 cut(s) 843
EcoO109I RGGNCCY 2 cut(s) 506, 788
EcoT22I ATGCAT 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 840
FaeI CATG 5 cut(s) 161, 303, 420, 438, 787
FaqI GGGAC 2 cut(s) 210, 774
FatI CATG 5 cut(s) 157, 299, 416, 434, 783
FauI CCCGC 1 cut(s) 767
FblI GTMKAC 1 cut(s) 730
Fnu4HI GCNGC 3 cut(s) 207, 772, 834
FokI GGATG 2 cut(s) 125, 451
Fsp4HI GCNGC 3 cut(s) 207, 772, 834
FspBI CTAG 2 cut(s) 90, 255
GluI GCNGC 3 cut(s) 207, 772, 834
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 1 cut(s) 822
HgaI GACGC 1 cut(s) 8
Hin1II CATG 5 cut(s) 161, 303, 420, 438, 787
HinfI GANTC 5 cut(s) 61, 135, 526, 532, 665
Hpy166II GTNNAC 2 cut(s) 266, 731
Hpy188I TCNGA 8 cut(s) 220, 280, 390, 531, 664, 680, 816, 867
Hpy188III TCNNGA 2 cut(s) 65, 843
Hpy8I GTNNAC 2 cut(s) 266, 731
HpyAV CCTTC 2 cut(s) 146, 155
HpyCH4III ACNGT 3 cut(s) 7, 232, 261
HpyCH4V TGCA 8 cut(s) 209, 295, 373, 416, 516, 562, 596, 833
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 602, 649
HpyF3I CTNAG 1 cut(s) 753
Hsp92II CATG 5 cut(s) 161, 303, 420, 438, 787
KflI GGGWCCC 1 cut(s) 788
Kzo9I GATC 5 cut(s) 40, 97, 425, 745, 839
LmnI GCTCC 2 cut(s) 84, 640
LpnPI CCDG 8 cut(s) 69, 78, 114, 188, 299, 620, 719, 822
Lsp1109I GCAGC 2 cut(s) 193, 845
LweI GCATC 2 cut(s) 382, 403
MaeI CTAG 2 cut(s) 90, 255
MaeIII GTNAC 2 cut(s) 58, 544
MalI GATC 5 cut(s) 42, 99, 427, 747, 841
MboI GATC 5 cut(s) 40, 97, 425, 745, 839
MboII GAAGA 4 cut(s) 107, 233, 864, 899
MflI RGATCY 2 cut(s) 425, 839
MlsI TGGCCA 1 cut(s) 822
MluCI AATT 8 cut(s) 146, 187, 237, 272, 359, 574, 861, 870
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 3 cut(s) 55, 526, 674
MmeI TCCRAC 1 cut(s) 685
MnlI CCTC 9 cut(s) 92, 125, 226, 274, 497, 519, 642, 682, 701
Mox20I TGGCCA 1 cut(s) 822
Mph1103I ATGCAT 1 cut(s) 418
MscI TGGCCA 1 cut(s) 822
MseI TTAA 1 cut(s) 627
MslI CAYNNNNRTG 1 cut(s) 587
Msp20I TGGCCA 1 cut(s) 822
MwoI GCNNNNNNNGC 3 cut(s) 212, 602, 649
NdeII GATC 5 cut(s) 40, 97, 425, 745, 839
NlaIII CATG 5 cut(s) 161, 303, 420, 438, 787
NlaIV GGNNCC 4 cut(s) 617, 789, 790, 841
NmuCI GTSAC 2 cut(s) 58, 544
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 1 cut(s) 420
PfeI GAWTC 2 cut(s) 135, 526
PkrI GCNGC 3 cut(s) 208, 773, 835
PleI GAGTC 3 cut(s) 55, 526, 673
PpsI GAGTC 3 cut(s) 55, 526, 673
PpuMI RGGWCCY 2 cut(s) 506, 788
Psp5II RGGWCCY 2 cut(s) 506, 788
PspN4I GGNNCC 4 cut(s) 617, 789, 790, 841
PspPI GGNCC 3 cut(s) 53, 506, 788
PspPPI RGGWCCY 2 cut(s) 506, 788
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 2 cut(s) 425, 839
RsaI GTAC 4 cut(s) 229, 267, 365, 751
RsaNI GTAC 4 cut(s) 228, 266, 364, 750
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 1 cut(s) 627
SatI GCNGC 3 cut(s) 207, 772, 834
Sau3AI GATC 5 cut(s) 40, 97, 425, 745, 839
Sau96I GGNCC 3 cut(s) 53, 506, 788
SchI GAGTC 3 cut(s) 55, 526, 674
SetI ASST 4 cut(s) 411, 508, 621, 645
SfaNI GCATC 2 cut(s) 382, 403
SinI GGWCC 3 cut(s) 53, 506, 788
SmiMI CAYNNNNRTG 1 cut(s) 587
Sse9I AATT 8 cut(s) 146, 187, 237, 272, 359, 574, 861, 870
SsiI CCGC 4 cut(s) 213, 603, 771, 774
SspMI CTAG 2 cut(s) 90, 255
TaaI ACNGT 3 cut(s) 7, 232, 261
TasI AATT 8 cut(s) 146, 187, 237, 272, 359, 574, 861, 870
TatI WGTACW 2 cut(s) 227, 265
TauI GCSGC 1 cut(s) 774
TfiI GAWTC 2 cut(s) 135, 526
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TscAI CASTG 8 cut(s) 12, 63, 91, 109, 237, 266, 318, 803
TseFI GTSAC 2 cut(s) 58, 544
TseI GCWGC 2 cut(s) 206, 833
Tsp45I GTSAC 2 cut(s) 58, 544
TspDTI ATGAA 3 cut(s) 127, 174, 528
TspGWI ACGGA 1 cut(s) 864
TspRI CASTG 8 cut(s) 12, 63, 91, 109, 237, 266, 318, 803
VpaK11BI GGWCC 3 cut(s) 53, 506, 788
XapI RAATTY 4 cut(s) 146, 187, 237, 272
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 730
XspI CTAG 2 cut(s) 90, 255
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.