Rorug02G0448100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007555
Physical Location & Seq
Reverse (-)
57493863 .. 57517347
23485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0448100.1

Sequence Viewer

Length: 1083 bp
ATGGCAGCTTTGAAGTTCCATATTCTTATCTTGGCCTCCTGGGCAACTCTTCTTATTCCTTGCAGCTCCCATGGCGATTCAGGGCTTCAGAAAAGACATGCAGCCCTGTACGTGTTTGGTGATTCATTATTTGACCCTGGAAACAATAACTACATAAGTACCACCAATGATTACAAGGCAGATTGGTCCCCTTATGGTGAAACTTTCTTCAAGTACCCAACTGGCAGATTTTGCGACGGACGTCTAATCCCAGATTTCATTGCTGAGTATGCAAAGTTGCCAATTGTACCGGCATTTTATCCGAGGATTGTGGACTATACTTATGGGGTCAACTTTGCTTCTGGTGGAGCTGGTTCTCTAAATGAAACACATAAAGGATTCGTGATACCCCTTAACGATCAACTGCATAATTTCAAGAATATGGAGAAGCATTTGAGGCACAATCTAGGTGATGCAAAAACCTACACATTGCTGTCAAATGCTGTTTACTTGATCAGCATTGGAGGCAATGACTATTTCACTCCATTCGCGACAAATTGTAGTTTCTTTGAGACTGACTCACAAGAGGAATATGTTGGCATTGTGATTGGAAACCTCACAAATGTGATCAAAGAAATATACAATAAAGGAGGACGGAAATTCGGGTTTACAAAATTGCCACCTCTGGGTCTTGTGCCATCCCAGAGACCAGGAAACAAAGGCACCTGTGTTAAAGAAGTTACATCGCTAGCAAACATACACAATGCAGCACTTCCTAAACACCTTGAAAAGCTGAAGAGCAAGCTGCAAGGATTCAAATATTCAATTGCAAAATTAGACACTTACCTAACTGAAAGAATCAATAAACCATCTAAATATGGTTTCAAGGAAGGAAAGAGTGCATGCTGTGGCTCTGGTCCATATGGAGGAATTAAAAGCTGTGGAGGGAAGAGAGGTGTGACCAAGTATCAGTTATGTGCCAATGTTAGTGAGGATGTCTTTTTTGACTCTTTCCATCCAACAGAAAGGGTTTACCAGCAACTTTCCGAGCTATGGTACCGTGGAACCAAGTACCTAATGCCAAAGAGCCTTACAGTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

40.12

Weight (kDa)

9.08

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 344 1.9e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 1035
AccB1I GGYRCC 2 cut(s) 701, 1035
AccII CGCG 1 cut(s) 530
AcsI RAATTY 1 cut(s) 638
AcuI CTGAAG 2 cut(s) 71, 794
AcyI GRCGYC 1 cut(s) 241
AfaI GTAC 6 cut(s) 110, 160, 215, 288, 1037, 1052
AfiI CCNNNNNNNGG 3 cut(s) 665, 905, 1032
AflIII ACRYGT 1 cut(s) 111
AgsI TTSAA 7 cut(s) 13, 211, 415, 767, 796, 804, 865
AjnI CCWGG 3 cut(s) 38, 136, 688
AleI CACNNNNGTG 1 cut(s) 602
AluBI AGCT 7 cut(s) 8, 66, 350, 772, 784, 918, 1030
AluI AGCT 7 cut(s) 8, 66, 350, 772, 784, 918, 1030
Alw26I GTCTC 2 cut(s) 545, 679
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 5 cut(s) 5, 63, 101, 746, 784
ApoI RAATTY 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 1035
AspS9I GGNCC 2 cut(s) 186, 896
AsuHPI GGTGA 3 cut(s) 131, 209, 461
AsuNHI GCTAGC 1 cut(s) 727
AvaII GGWCC 2 cut(s) 186, 896
BaeI ACNNNNGTAYC 2 cut(s) 142, 175
BanI GGYRCC 2 cut(s) 701, 1035
BbvI GCAGC 5 cut(s) 17, 75, 113, 758, 771
BccI CCATC 3 cut(s) 685, 856, 1002
BciT130I CCWGG 3 cut(s) 40, 138, 690
BclI TGATCA 2 cut(s) 492, 606
BcoDI GTCTC 2 cut(s) 545, 679
BfaI CTAG 2 cut(s) 446, 728
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 5 cut(s) 6, 64, 102, 747, 785
BlsI GCNGC 5 cut(s) 7, 65, 103, 748, 786
Bme1390I CCNGG 3 cut(s) 40, 138, 690
Bme18I GGWCC 2 cut(s) 186, 896
BmgT120I GGNCC 2 cut(s) 186, 896
BmiI GGNNCC 4 cut(s) 188, 703, 1037, 1045
BmrFI CCNGG 3 cut(s) 40, 138, 690
BmsI GCATC 1 cut(s) 442
BmtI GCTAGC 1 cut(s) 731
BoxI GACNNNNGTC 1 cut(s) 240
BplI GAGNNNNNCTC 2 cut(s) 542, 574
BsaAI YACGTR 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 679
BsaJI CCNNGG 5 cut(s) 39, 70, 136, 302, 1039
Bsc4I CCNNNNNNNGG 3 cut(s) 665, 905, 1032
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 226
Bse3DI GCAATG 3 cut(s) 258, 467, 514
BseBI CCWGG 3 cut(s) 40, 138, 690
BseDI CCNNGG 5 cut(s) 39, 70, 136, 302, 1039
BseGI GGATG 3 cut(s) 677, 979, 994
BseLI CCNNNNNNNGG 3 cut(s) 665, 905, 1032
BseMI GCAATG 3 cut(s) 258, 467, 514
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 226
BseXI GCAGC 5 cut(s) 17, 75, 113, 758, 771
Bsh1236I CGCG 1 cut(s) 530
BshFI GGCC 1 cut(s) 35
BshNI GGYRCC 2 cut(s) 701, 1035
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 3 cut(s) 665, 905, 1032
BsmAI GTCTC 2 cut(s) 545, 679
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 35
Bso31I GGTCTC 1 cut(s) 679
Bsp143I GATC 3 cut(s) 397, 492, 606
Bsp19I CCATGG 1 cut(s) 70
Bsp68I TCGCGA 1 cut(s) 530
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 530
BspLI GGNNCC 4 cut(s) 188, 703, 1037, 1045
BspOI GCTAGC 1 cut(s) 731
BspQI GCTCTTC 1 cut(s) 770
BspT107I GGYRCC 2 cut(s) 701, 1035
BspTNI GGTCTC 1 cut(s) 679
BsrDI GCAATG 3 cut(s) 258, 467, 514
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 5 cut(s) 39, 70, 136, 302, 1039
BssMI GATC 3 cut(s) 397, 492, 606
BssNI GRCGYC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 3 cut(s) 40, 138, 690
Bst4CI ACNGT 2 cut(s) 1040, 1075
Bst6I CTCTTC 3 cut(s) 54, 770, 923
BstACI GRCGYC 1 cut(s) 241
BstAPI GCANNNNNTGC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 112
BstC8I GCNNGC 3 cut(s) 729, 782, 883
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 2 cut(s) 70, 1039
BstF5I GGATG 3 cut(s) 677, 979, 994
BstFNI CGCG 1 cut(s) 530
BstKTI GATC 3 cut(s) 400, 495, 609
BstMAI GTCTC 2 cut(s) 545, 679
BstMBI GATC 3 cut(s) 397, 492, 606
BstMWI GCNNNNNNNGC 6 cut(s) 41, 72, 231, 269, 436, 504
BstNI CCWGG 3 cut(s) 40, 138, 690
BstNSI RCATGY 2 cut(s) 101, 885
BstPAI GACNNNNGTC 1 cut(s) 240
BstSCI CCNGG 3 cut(s) 38, 136, 688
BstUI CGCG 1 cut(s) 530
BstV1I GCAGC 5 cut(s) 17, 75, 113, 758, 771
BsuRI GGCC 1 cut(s) 35
BtgI CCRYGG 2 cut(s) 70, 1039
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 3 cut(s) 677, 979, 994
BtuMI TCGCGA 1 cut(s) 530
Cac8I GCNNGC 3 cut(s) 729, 782, 883
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 186, 896
Csp6I GTAC 6 cut(s) 109, 159, 214, 287, 1036, 1051
CviAII CATG 3 cut(s) 71, 98, 882
CviQI GTAC 6 cut(s) 109, 159, 214, 287, 1036, 1051
DdeI CTNAG 1 cut(s) 264
DpnI GATC 3 cut(s) 399, 494, 608
DpnII GATC 3 cut(s) 397, 492, 606
Eam1104I CTCTTC 3 cut(s) 54, 770, 923
EarI CTCTTC 3 cut(s) 54, 770, 923
Eco130I CCWWGG 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 679
Eco47I GGWCC 2 cut(s) 186, 896
Eco57I CTGAAG 2 cut(s) 71, 794
EcoRII CCWGG 3 cut(s) 38, 136, 688
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 3 cut(s) 74, 101, 885
FaqI GGGAC 1 cut(s) 172
FatI CATG 3 cut(s) 70, 97, 881
FauNDI CATATG 1 cut(s) 901
FbaI TGATCA 2 cut(s) 492, 606
Fnu4HI GCNGC 5 cut(s) 6, 64, 102, 747, 785
FokI GGATG 3 cut(s) 664, 981, 986
Fsp4HI GCNGC 5 cut(s) 6, 64, 102, 747, 785
FspBI CTAG 2 cut(s) 446, 728
GluI GCNGC 5 cut(s) 6, 64, 102, 747, 785
HaeIII GGCC 1 cut(s) 35
HapII CCGG 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 241
Hin1II CATG 3 cut(s) 74, 101, 885
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 7 cut(s) 77, 122, 378, 557, 792, 837, 986
HpaII CCGG 1 cut(s) 290
HphI GGTGA 3 cut(s) 131, 209, 461
Hpy166II GTNNAC 5 cut(s) 313, 331, 487, 648, 1012
Hpy188I TCNGA 4 cut(s) 90, 303, 1027, 1082
Hpy188III TCNNGA 3 cut(s) 382, 415, 529
Hpy8I GTNNAC 5 cut(s) 313, 331, 487, 648, 1012
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 863
HpyCH4III ACNGT 2 cut(s) 1040, 1075
HpyCH4IV ACGT 2 cut(s) 111, 241
HpyCH4V TGCA 9 cut(s) 63, 101, 272, 406, 455, 746, 787, 809, 881
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 72, 231, 269, 436, 504
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 2 cut(s) 111, 241
Hsp92I GRCGYC 1 cut(s) 241
Hsp92II CATG 3 cut(s) 74, 101, 885
KpnI GGTACC 1 cut(s) 1039
Ksp22I TGATCA 2 cut(s) 492, 606
Kzo9I GATC 3 cut(s) 397, 492, 606
LguI GCTCTTC 1 cut(s) 770
LmnI GCTCC 2 cut(s) 71, 347
Lsp1109I GCAGC 5 cut(s) 17, 75, 113, 758, 771
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 446, 728
MaeII ACGT 2 cut(s) 111, 241
MaeIII GTNAC 2 cut(s) 718, 937
MalI GATC 3 cut(s) 399, 494, 608
MboI GATC 3 cut(s) 397, 492, 606
MboII GAAGA 4 cut(s) 41, 199, 787, 940
MfeI CAATTG 2 cut(s) 282, 804
MluCI AATT 8 cut(s) 282, 409, 535, 638, 653, 804, 812, 909
MlyI GAGTC 2 cut(s) 551, 980
MmeI TCCRAC 1 cut(s) 1022
MseI TTAA 3 cut(s) 393, 711, 912
MslI CAYNNNNRTG 1 cut(s) 602
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 3 cut(s) 40, 138, 690
MunI CAATTG 2 cut(s) 282, 804
MvaI CCWGG 3 cut(s) 40, 138, 690
MvnI CGCG 1 cut(s) 530
MwoI GCNNNNNNNGC 6 cut(s) 41, 72, 231, 269, 436, 504
NcoI CCATGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 901
NdeII GATC 3 cut(s) 397, 492, 606
NheI GCTAGC 1 cut(s) 727
NlaIII CATG 3 cut(s) 74, 101, 885
NlaIV GGNNCC 4 cut(s) 188, 703, 1037, 1045
NmuCI GTSAC 1 cut(s) 937
NruI TCGCGA 1 cut(s) 530
NspI RCATGY 2 cut(s) 101, 885
OliI CACNNNNGTG 1 cut(s) 602
PaeI GCATGC 1 cut(s) 885
PciSI GCTCTTC 1 cut(s) 770
PfeI GAWTC 5 cut(s) 77, 122, 378, 792, 837
PkrI GCNGC 5 cut(s) 7, 65, 103, 748, 786
PleI GAGTC 2 cut(s) 551, 980
PpsI GAGTC 2 cut(s) 551, 980
Ppu21I YACGTR 1 cut(s) 112
PshAI GACNNNNGTC 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 38, 136, 688
PspGI CCWGG 3 cut(s) 38, 136, 688
PspN4I GGNNCC 4 cut(s) 188, 703, 1037, 1045
PspPI GGNCC 2 cut(s) 186, 896
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 6 cut(s) 110, 160, 215, 288, 1037, 1052
RsaNI GTAC 6 cut(s) 109, 159, 214, 287, 1036, 1051
RseI CAYNNNNRTG 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 770
SaqAI TTAA 3 cut(s) 393, 711, 912
SatI GCNGC 5 cut(s) 6, 64, 102, 747, 785
Sau3AI GATC 3 cut(s) 397, 492, 606
Sau96I GGNCC 2 cut(s) 186, 896
SchI GAGTC 2 cut(s) 551, 980
ScrFI CCNGG 3 cut(s) 40, 138, 690
SfaNI GCATC 1 cut(s) 442
SinI GGWCC 2 cut(s) 186, 896
SmiMI CAYNNNNRTG 1 cut(s) 602
SphI GCATGC 1 cut(s) 885
Sse9I AATT 8 cut(s) 282, 409, 535, 638, 653, 804, 812, 909
SspI AATATT 1 cut(s) 800
SspMI CTAG 2 cut(s) 446, 728
StyD4I CCNGG 3 cut(s) 38, 136, 688
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 1040, 1075
TaiI ACGT 2 cut(s) 114, 244
TasI AATT 8 cut(s) 282, 409, 535, 638, 653, 804, 812, 909
TfiI GAWTC 5 cut(s) 77, 122, 378, 792, 837
Tru1I TTAA 3 cut(s) 393, 711, 912
Tru9I TTAA 3 cut(s) 393, 711, 912
TseFI GTSAC 1 cut(s) 937
TseI GCWGC 5 cut(s) 5, 63, 101, 746, 784
Tsp45I GTSAC 1 cut(s) 937
TspDTI ATGAA 3 cut(s) 114, 247, 378
TspGWI ACGGA 2 cut(s) 252, 649
VpaK11BI GGWCC 2 cut(s) 186, 896
XapI RAATTY 1 cut(s) 638
XceI RCATGY 2 cut(s) 101, 885
XspI CTAG 2 cut(s) 446, 728
ZraI GACGTC 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.