Rh3AG163500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007555
Physical Location & Seq
Reverse (-)
15080402 .. 15096046
15645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG163500.1

Sequence Viewer

Length: 411 bp
ATGCAAAGTATTCAAGGCTACGAGACCTGTAGTTGTGGCCTTTCTAGCCCAAGGCCCGAGTCCGAATTTTGGGAGTTCAAGGGTATGGTATTTTTGTCGGCAAAAATGGTCAAAAAAATTGAGAAGACGCATGTTTGCGACGCCTTCAAAAGAACACAATCCGATGACCCGGATCATCGACGAACAAGTCCTCAGGACGCATCCATCGGTGCTGCTGTCCAGCTCCCTGATACCAACATCCGGATCTGGGATCCTGACCTGTCGAACGAATTACAAACATTGAACGTGGGTATTCTCGACATTGAACGCCACCATCATATTGCTGGTTCTGTATTGTTTGGGACCCCAGATTTTGGAATCCCATATTGTCATATGGCCTATGAAAACTCTGGGGCAACTTTAATTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.19

Weight (kDa)

5.39

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 353
AccIII TCCGGA 1 cut(s) 240
AclWI GGATC 4 cut(s) 180, 245, 251, 258
AcsI RAATTY 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 141
AfiI CCNNNNNNNGG 4 cut(s) 69, 240, 247, 353
AgsI TTSAA 5 cut(s) 14, 79, 148, 283, 305
AluBI AGCT 1 cut(s) 223
AluI AGCT 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 4 cut(s) 180, 245, 251, 258
Ama87I CYCGRG 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 3 cut(s) 37, 53, 375
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 54, 342
AsuC2I CCSGG 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 56
AvaII GGWCC 1 cut(s) 342
AxyI CCTNAGG 1 cut(s) 192
BamHI GGATCC 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 199
BccI CCATC 2 cut(s) 212, 321
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 1 cut(s) 45
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 1 cut(s) 342
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 2 cut(s) 54, 342
BmiI GGNNCC 3 cut(s) 252, 343, 344
BmrFI CCNGG 1 cut(s) 170
BmsI GCATC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 240
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 240, 247, 353
Bse21I CCTNAGG 1 cut(s) 192
BseAI TCCGGA 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 2 cut(s) 200, 237
BseLI CCNNNNNNNGG 4 cut(s) 69, 240, 247, 353
BseMII CTCAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 199
BshFI GGCC 3 cut(s) 39, 55, 377
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 2 cut(s) 170, 241
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 4 cut(s) 69, 240, 247, 353
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 3 cut(s) 39, 55, 377
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 56
Bsp13I TCCGGA 1 cut(s) 240
Bsp143I GATC 3 cut(s) 172, 243, 250
BspANI GGCC 3 cut(s) 39, 55, 377
BspCNI CTCAG 1 cut(s) 205
BspEI TCCGGA 1 cut(s) 240
BspLI GGNNCC 3 cut(s) 252, 343, 344
BspPI GGATC 4 cut(s) 180, 245, 251, 258
BspTNI GGTCTC 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 3 cut(s) 172, 243, 250
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 141
BstDEI CTNAG 1 cut(s) 192
BstF5I GGATG 2 cut(s) 200, 237
BstKTI GATC 3 cut(s) 175, 246, 253
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 3 cut(s) 172, 243, 250
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 2 cut(s) 243, 250
BstYI RGATCY 2 cut(s) 243, 250
Bsu36I CCTNAGG 1 cut(s) 192
BsuRI GGCC 3 cut(s) 39, 55, 377
BtsCI GGATG 2 cut(s) 200, 237
Cfr13I GGNCC 2 cut(s) 54, 342
CseI GACGC 3 cut(s) 136, 149, 206
CviAII CATG 1 cut(s) 131
CviJI RGCY 6 cut(s) 18, 39, 48, 55, 223, 377
CviKI_1 RGCY 6 cut(s) 18, 39, 48, 55, 223, 377
DdeI CTNAG 1 cut(s) 192
DpnI GATC 3 cut(s) 174, 245, 252
DpnII GATC 3 cut(s) 172, 243, 250
DrdI GACNNNNNNGTC 1 cut(s) 186
DseDI GACNNNNNNGTC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 342
Eco81I CCTNAGG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 1 cut(s) 134
FaiI YATR 8 cut(s) 86, 132, 318, 364, 372, 374, 381, 409
FaqI GGGAC 1 cut(s) 355
FatI CATG 1 cut(s) 130
FauNDI CATATG 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 2 cut(s) 187, 224
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 45
GluI GCNGC 1 cut(s) 213
HaeIII GGCC 3 cut(s) 39, 55, 377
HapII CCGG 2 cut(s) 170, 241
HgaI GACGC 3 cut(s) 136, 149, 206
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 1 cut(s) 134
HinfI GANTC 2 cut(s) 59, 357
HpaII CCGG 2 cut(s) 170, 241
Hpy188I TCNGA 2 cut(s) 64, 163
Hpy188III TCNNGA 4 cut(s) 194, 241, 254, 296
Hpy99I CGWCG 2 cut(s) 143, 183
HpyAV CCTTC 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 1 cut(s) 134
KflI GGGWCCC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 240
Kzo9I GATC 3 cut(s) 172, 243, 250
LmnI GCTCC 1 cut(s) 228
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 1 cut(s) 209
MaeI CTAG 1 cut(s) 45
MaeII ACGT 1 cut(s) 285
MalI GATC 3 cut(s) 174, 245, 252
MboI GATC 3 cut(s) 172, 243, 250
MboII GAAGA 1 cut(s) 136
MflI RGATCY 2 cut(s) 243, 250
MluCI AATT 4 cut(s) 65, 117, 269, 402
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 1 cut(s) 201
MroI TCCGGA 1 cut(s) 240
MseI TTAA 1 cut(s) 401
MspI CCGG 2 cut(s) 170, 241
MspR9I CCNGG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 45
NciI CCSGG 1 cut(s) 170
NdeI CATATG 1 cut(s) 372
NdeII GATC 3 cut(s) 172, 243, 250
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 3 cut(s) 252, 343, 344
NspI RCATGY 1 cut(s) 134
PfeI GAWTC 1 cut(s) 357
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
PspN4I GGNNCC 3 cut(s) 252, 343, 344
PspPI GGNCC 2 cut(s) 54, 342
PspPPI RGGWCCY 1 cut(s) 342
PsuI RGATCY 2 cut(s) 243, 250
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 3 cut(s) 172, 243, 250
Sau96I GGNCC 2 cut(s) 54, 342
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 170
SetI ASST 4 cut(s) 29, 225, 261, 288
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 1 cut(s) 342
Sse9I AATT 4 cut(s) 65, 117, 269, 402
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 168
StyI CCWWGG 1 cut(s) 50
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 178, 263, 297
TasI AATT 4 cut(s) 65, 117, 269, 402
TfiI GAWTC 1 cut(s) 357
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 396
Van91I CCANNNNNTGG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 1 cut(s) 65
XceI RCATGY 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 320
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.