Rh4DG175900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007555
Physical Location & Seq
Reverse (-)
34950983 .. 34953063
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG175900.1

Sequence Viewer

Length: 492 bp
ATGGGTAGCGACGATCTCAGTCTCGAGGTGAGATCGGTCGTCATGCTAAGGTCTACTCCTGCTATTACAGAGAAAAAAGTTTCTTTTGCTAGAATTTTTGTCTTGGGGTTCCCAAAGGTGAATTTTGATAGGGAAACTGTTCTTTTCGATTGGCTGGAGTCAGTCGACCACGGTGGTTTTTTAGAATGCAGTGATGACTGCTTTGGAGAGACTCCGCATCTAAGAGGCTTGAAAATTAGAATCGGTGTTATGCAGTTGGACGTTGACGGTTTTGTGGTGTGGGCGCCAAGTCATGGCTACGATGCCAATGCTGGGTTCTTAGGAGTGGACGCCGGAGACTATGGGTCCTTTCATGGCTCCTTAGAGTCAGTCATTTTCTGGTGTGGCGAGCTCAATGTTGTGGGTGGTTGGGGTCCCGGTGATGGGTTCTCTCTAGTTGTAGTGGTGGTGGTGGCAGAGATGCGATGCTTTCAAGCTGTGACGGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.68

Weight (kDa)

4.55

Isoelectric Point (pI)

20.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 283
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 2 cut(s) 53, 165
AciI CCGC 1 cut(s) 215
AcsI RAATTY 2 cut(s) 93, 121
AcyI GRCGYC 2 cut(s) 284, 330
AfiI CCNNNNNNNGG 4 cut(s) 293, 312, 422, 423
AgsI TTSAA 2 cut(s) 232, 473
AhdI GACNNNNNGTC 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 3 cut(s) 391, 476, 489
AluI AGCT 3 cut(s) 391, 476, 489
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 3 cut(s) 26, 203, 330
Ama87I CYCGRG 1 cut(s) 23
ApoI RAATTY 2 cut(s) 93, 121
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 345, 413
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 3 cut(s) 40, 130, 431
AsuNHI GCTAGC 1 cut(s) 485
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 2 cut(s) 345, 413
BanI GGYRCC 1 cut(s) 283
BanII GRGCYC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 393
BccI CCATC 1 cut(s) 416
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 3 cut(s) 26, 203, 330
BfaI CTAG 4 cut(s) 90, 434, 486, 490
BfoI RGCGCY 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 2 cut(s) 345, 413
BmeRI GACNNNNNGTC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 345, 413
BmiI GGNNCC 6 cut(s) 110, 285, 346, 358, 414, 415
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 4 cut(s) 226, 292, 450, 455
BmtI GCTAGC 1 cut(s) 489
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 417
BsaHI GRCGYC 2 cut(s) 284, 330
BsaJI CCNNGG 1 cut(s) 169
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 312, 422, 423
BseDI CCNNGG 1 cut(s) 169
BseLI CCNNNNNNNGG 4 cut(s) 293, 312, 422, 423
BseMII CTCAG 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 39
BshNI GGYRCC 1 cut(s) 283
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 2 cut(s) 333, 417
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 4 cut(s) 293, 312, 422, 423
BsmAI GTCTC 3 cut(s) 26, 203, 330
BsmFI GGGAC 1 cut(s) 399
BsmI GAATGC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 2 cut(s) 13, 32
BspACI CCGC 1 cut(s) 215
BspCNI CTCAG 1 cut(s) 30
BspLI GGNNCC 6 cut(s) 110, 285, 346, 358, 414, 415
BspOI GCTAGC 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 2 cut(s) 13, 32
BssNI GRCGYC 2 cut(s) 284, 330
Bst4CI ACNGT 3 cut(s) 139, 173, 269
BstACI GRCGYC 2 cut(s) 284, 330
BstC8I GCNNGC 2 cut(s) 389, 487
BstDEI CTNAG 5 cut(s) 17, 47, 221, 319, 361
BstDSI CCRYGG 1 cut(s) 169
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 2 cut(s) 16, 35
BstMAI GTCTC 3 cut(s) 26, 203, 330
BstMBI GATC 2 cut(s) 13, 32
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 415
BtgI CCRYGG 1 cut(s) 169
BtgZI GCGATG 1 cut(s) 478
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 389, 487
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 2 cut(s) 345, 413
CseI GACGC 1 cut(s) 338
CviAII CATG 3 cut(s) 43, 293, 353
CviJI RGCY 8 cut(s) 154, 228, 297, 357, 391, 476, 485, 489
CviKI_1 RGCY 8 cut(s) 154, 228, 297, 357, 391, 476, 485, 489
DdeI CTNAG 5 cut(s) 17, 47, 221, 319, 361
DinI GGCGCC 1 cut(s) 285
DpnI GATC 2 cut(s) 15, 34
DpnII GATC 2 cut(s) 13, 32
DriI GACNNNNNGTC 1 cut(s) 343
Eam1105I GACNNNNNGTC 1 cut(s) 343
Ecl136II GAGCTC 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 393
Eco47I GGWCC 2 cut(s) 345, 413
Eco53kI GAGCTC 1 cut(s) 391
Eco88I CYCGRG 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 391
EcoO109I RGGNCCY 2 cut(s) 345, 413
EcoT38I GRGCYC 1 cut(s) 393
EgeI GGCGCC 1 cut(s) 285
EheI GGCGCC 1 cut(s) 285
FaeI CATG 3 cut(s) 46, 296, 356
FaiI YATR 5 cut(s) 44, 251, 294, 342, 354
FaqI GGGAC 1 cut(s) 399
FatI CATG 3 cut(s) 42, 292, 352
FblI GTMKAC 2 cut(s) 53, 165
FriOI GRGCYC 1 cut(s) 393
FspBI CTAG 4 cut(s) 90, 434, 486, 490
GlaI GCGC 1 cut(s) 285
GsaI CCCAGC 1 cut(s) 315
GsuI CTGGAG 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 287
HapII CCGG 2 cut(s) 333, 417
HgaI GACGC 1 cut(s) 338
HhaI GCGC 1 cut(s) 286
Hin1I GRCGYC 2 cut(s) 284, 330
Hin1II CATG 3 cut(s) 46, 296, 356
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 2 cut(s) 166, 265
HindII GTYRAC 2 cut(s) 166, 265
HinfI GANTC 4 cut(s) 158, 211, 240, 365
HpaII CCGG 2 cut(s) 333, 417
HphI GGTGA 3 cut(s) 40, 130, 431
Hpy166II GTNNAC 4 cut(s) 54, 166, 265, 328
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 4 cut(s) 54, 166, 265, 328
Hpy99I CGWCG 1 cut(s) 14
HpyCH4III ACNGT 3 cut(s) 139, 173, 269
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 2 cut(s) 189, 253
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 5 cut(s) 17, 47, 221, 319, 361
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 2 cut(s) 284, 330
Hsp92II CATG 3 cut(s) 46, 296, 356
HspAI GCGC 1 cut(s) 284
KasI GGCGCC 1 cut(s) 283
KflI GGGWCCC 1 cut(s) 413
Kzo9I GATC 2 cut(s) 13, 32
LmnI GCTCC 1 cut(s) 362
LpnPI CCDG 6 cut(s) 72, 140, 297, 346, 364, 430
LweI GCATC 4 cut(s) 226, 292, 450, 455
MaeI CTAG 4 cut(s) 90, 434, 486, 490
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 478
MalI GATC 2 cut(s) 15, 34
MboI GATC 2 cut(s) 13, 32
MhlI GDGCHC 1 cut(s) 393
MluCI AATT 3 cut(s) 93, 121, 234
Mly113I GGCGCC 1 cut(s) 284
MlyI GAGTC 3 cut(s) 167, 205, 374
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 2 cut(s) 19, 218
MroXI GAANNNNTTC 1 cut(s) 138
MspI CCGG 2 cut(s) 333, 417
MspR9I CCNGG 1 cut(s) 417
Mva1269I GAATGC 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 482
NarI GGCGCC 1 cut(s) 284
NciI CCSGG 1 cut(s) 417
NdeII GATC 2 cut(s) 13, 32
NheI GCTAGC 1 cut(s) 485
NlaIII CATG 3 cut(s) 46, 296, 356
NlaIV GGNNCC 6 cut(s) 110, 285, 346, 358, 414, 415
NmuCI GTSAC 1 cut(s) 478
PaeR7I CTCGAG 1 cut(s) 23
PctI GAATGC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 293
PleI GAGTC 3 cut(s) 166, 205, 373
PluTI GGCGCC 1 cut(s) 287
PpsI GAGTC 3 cut(s) 166, 205, 373
PpuMI RGGWCCY 2 cut(s) 345, 413
Psp124BI GAGCTC 1 cut(s) 393
Psp5II RGGWCCY 2 cut(s) 345, 413
PspFI CCCAGC 1 cut(s) 311
PspN4I GGNNCC 6 cut(s) 110, 285, 346, 358, 414, 415
PspPI GGNCC 2 cut(s) 345, 413
PspPPI RGGWCCY 2 cut(s) 345, 413
SacI GAGCTC 1 cut(s) 393
SalI GTCGAC 1 cut(s) 164
Sau3AI GATC 2 cut(s) 13, 32
Sau96I GGNCC 2 cut(s) 345, 413
SchI GAGTC 3 cut(s) 167, 205, 374
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 393
SetI ASST 7 cut(s) 30, 53, 120, 264, 393, 478, 491
SfaNI GCATC 4 cut(s) 226, 292, 450, 455
SfoI GGCGCC 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 23
SinI GGWCC 2 cut(s) 345, 413
SlaI CTCGAG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 3 cut(s) 93, 121, 234
SsiI CCGC 1 cut(s) 215
SspDI GGCGCC 1 cut(s) 283
SspMI CTAG 4 cut(s) 90, 434, 486, 490
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 415
TaaI ACNGT 3 cut(s) 139, 173, 269
TaiI ACGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 24, 147, 165
TaqII GACCGA 1 cut(s) 25
TasI AATT 3 cut(s) 93, 121, 234
TfiI GAWTC 1 cut(s) 240
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 478
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 1 cut(s) 341
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 345, 413
XapI RAATTY 2 cut(s) 93, 121
XhoI CTCGAG 1 cut(s) 23
XmiI GTMKAC 2 cut(s) 53, 165
XmnI GAANNNNTTC 1 cut(s) 138
XspI CTAG 4 cut(s) 90, 434, 486, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.