RchiOBHm_Chr7g0226031

Aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003005
Physical Location & Seq
Forward (+)
49086750 .. 49089982
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20244

Sequence Viewer

Length: 1194 bp
ATGGAGAAATTGCGGAATGGTTATTTGTTTCCTGAGATTTCAAAGCTTGAGCTCCAACATATTGAGAAGTACCCAGATGCCAAATTGATAAGCCTTGGAATTGGTGATACAACAGAACCGCTACCAGAAATCATAACCTCAACCATGGCTAATTATGCACATGCTCTTTCAACTGCTGATGGTTATTCAGGGTATGGACCTGAGCAAGGCAACAAGGCATTGCGGAAGGCAATTGCAAAAGCATTCTATAAAGATCTACCAGTAAAAGACACGGAAATTTTTGTATCAGATGGCGCTCAGTGTGATATTACTCGCCTTCAGCTGCTTCTGGGTTCAAATGTGACGATAGCTGTGCAAGATCCATCCTTTCCAGCTTATATAGACTCAAGTGTCATAATAGGTCAGGCAGGTGAGATCAAAGATGAAGCTGGGAGGTATAGGAGCATTGAATACATGAACTGCAGTCCTCACAATAATTTTTTTCCGAACTTATCAACTACTTCAAGAGCAGATATTATATTCTTTTGCTCTCCAAACAATCCAACTGGTTATGCAGCAACAAGGAAGCAATTAGAGCAACTTGTAGAGTTTGCAAGGCAGAATGGATCAATTATCATATATGACTCTGCCTATTCATCTTATATCAGAGACGATAGTCCTCGGTCAATATACGAAATTCCTGGTGCCAGAGAGGTTGCCATTGAAATTTCATCATTCTCCAAATTTGCTGGGTTCACTGGCATCCGTCTAGGTTGGACGGTTGTCCCTGAGGAGCTCTTATACTCCAATGGTTTTCCTGTTATATATGACTTCAATCGTATTGTGTGCACTTGCTTTAATGGAGCATCTAATATTGCTCAAGCTGGTGGATTGGCATGTCTTTCTCCAGTAGGTTTCCAGGCTGTGCATTCTATGGTTGACTACTACATGGAGAATGCAAAGATATTGGTCGAGACATTTGCTTCTCTTGGTTATCAAGTATATGGTGGTGCAAATGCTCCCTACATTTGGGTGCATTTTCCTGGCTCAAGTTCTTGGGATGTATTTACTGAGATTCTTGAAAAAACACACATCCTTACAGTTCCAGGTTCTGGATTTGGTCCAGCTGGTGAAGGGTGTCTCAGAATTAGTTCTTTTGGCCACAGAGAGTGTATCCGAGAGGCCTCGCGTAGGCTCAGAACCCTATTTCAATAG

Protein Analysis

397

Amino Acids

43.78

Weight (kDa)

5.38

Isoelectric Point (pI)

54.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 28 - 389 5.7e-38 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
AasI GACNNNNNNGTC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 398
AccB1I GGYRCC 1 cut(s) 683
AccB7I CCANNNNNTGG 1 cut(s) 1091
AccII CGCG 1 cut(s) 1168
AciI CCGC 3 cut(s) 13, 119, 223
AclWI GGATC 2 cut(s) 353, 613
AcoI YGGCCR 1 cut(s) 1138
AcsI RAATTY 4 cut(s) 276, 675, 705, 722
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 4 cut(s) 206, 1008, 1091, 1170
AgsI TTSAA 9 cut(s) 42, 171, 336, 449, 504, 704, 814, 1061, 1190
AjnI CCWGG 4 cut(s) 679, 897, 1021, 1084
AluBI AGCT 9 cut(s) 46, 52, 322, 350, 374, 428, 775, 863, 1106
AluI AGCT 9 cut(s) 46, 52, 322, 350, 374, 428, 775, 863, 1106
Alw21I GWGCWC 3 cut(s) 54, 777, 830
Alw26I GTCTC 3 cut(s) 642, 947, 1124
Alw44I GTGCAC 1 cut(s) 826
AlwI GGATC 2 cut(s) 353, 613
AlwNI CAGNNNCTG 1 cut(s) 1091
AoxI GGCC 2 cut(s) 1138, 1161
ApaLI GTGCAC 1 cut(s) 826
ApeKI GCWGC 2 cut(s) 322, 554
ApoI RAATTY 4 cut(s) 276, 675, 705, 722
Asp700I GAANNNNTTC 1 cut(s) 1129
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 2 cut(s) 197, 1100
AsuHPI GGTGA 3 cut(s) 116, 422, 1121
AvaII GGWCC 2 cut(s) 197, 1100
AxyI CCTNAGG 1 cut(s) 768
BaeGI GKGCMC 1 cut(s) 830
BalI TGGCCA 1 cut(s) 1140
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 2 cut(s) 54, 777
Bbv12I GWGCWC 3 cut(s) 54, 777, 830
BbvI GCAGC 2 cut(s) 309, 566
BccI CCATC 3 cut(s) 173, 284, 370
BcgI CGANNNNNNTGC 4 cut(s) 334, 368, 941, 975
BciT130I CCWGG 4 cut(s) 681, 899, 1023, 1086
BciVI GTATCC 1 cut(s) 1163
BcoDI GTCTC 3 cut(s) 642, 947, 1124
BfaI CTAG 1 cut(s) 749
BfmI CTRYAG 1 cut(s) 460
BfoI RGCGCY 1 cut(s) 297
BfuAI ACCTGC 1 cut(s) 398
BfuI GTATCC 1 cut(s) 1163
BglII AGATCT 1 cut(s) 253
BisI GCNGC 2 cut(s) 323, 555
BlsI GCNGC 2 cut(s) 324, 556
Bme1390I CCNGG 4 cut(s) 681, 899, 1023, 1086
Bme18I GGWCC 2 cut(s) 197, 1100
BmgT120I GGNCC 2 cut(s) 197, 1100
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 4 cut(s) 681, 899, 1023, 1086
BmsI GCATC 3 cut(s) 67, 750, 854
BoxI GACNNNNGTC 2 cut(s) 654, 761
BpmI CTGGAG 1 cut(s) 870
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 4 cut(s) 68, 370, 843, 1012
BsaJI CCNNGG 3 cut(s) 94, 144, 659
Bsc4I CCNNNNNNNGG 4 cut(s) 206, 1008, 1091, 1170
Bse1I ACTGG 4 cut(s) 260, 550, 742, 887
Bse21I CCTNAGG 1 cut(s) 768
Bse3DI GCAATG 1 cut(s) 218
BseBI CCWGG 4 cut(s) 681, 899, 1023, 1086
BseDI CCNNGG 3 cut(s) 94, 144, 659
BseGI GGATG 4 cut(s) 362, 741, 1045, 1071
BseLI CCNNNNNNNGG 4 cut(s) 206, 1008, 1091, 1170
BseMI GCAATG 1 cut(s) 218
BseMII CTCAG 7 cut(s) 24, 192, 311, 759, 1041, 1135, 1189
BseNI ACTGG 4 cut(s) 260, 550, 742, 887
BseRI GAGGAG 1 cut(s) 785
BseSI GKGCMC 1 cut(s) 830
BseXI GCAGC 2 cut(s) 309, 566
BseYI CCCAGC 2 cut(s) 428, 728
Bsh1236I CGCG 1 cut(s) 1168
BshFI GGCC 2 cut(s) 1140, 1163
BshNI GGYRCC 1 cut(s) 683
BsiHKAI GWGCWC 3 cut(s) 54, 777, 830
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 4 cut(s) 206, 1008, 1091, 1170
BsmAI GTCTC 3 cut(s) 642, 947, 1124
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 1 cut(s) 749
BsmI GAATGC 3 cut(s) 242, 907, 940
BsnI GGCC 2 cut(s) 1140, 1163
Bsp1286I GDGCHC 3 cut(s) 54, 777, 830
Bsp143I GATC 4 cut(s) 253, 358, 414, 605
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 3 cut(s) 13, 119, 223
BspANI GGCC 2 cut(s) 1140, 1163
BspCNI CTCAG 7 cut(s) 25, 193, 310, 760, 1042, 1134, 1188
BspFNI CGCG 1 cut(s) 1168
BspLI GGNNCC 1 cut(s) 685
BspMAI CTGCAG 1 cut(s) 464
BspMI ACCTGC 1 cut(s) 398
BspPI GGATC 2 cut(s) 353, 613
BspT107I GGYRCC 1 cut(s) 683
BsrDI GCAATG 1 cut(s) 218
BsrI ACTGG 4 cut(s) 260, 550, 742, 887
BssECI CCNNGG 3 cut(s) 94, 144, 659
BssMI GATC 4 cut(s) 253, 358, 414, 605
BssT1I CCWWGG 2 cut(s) 94, 144
Bst2UI CCWGG 4 cut(s) 681, 899, 1023, 1086
Bst4CI ACNGT 2 cut(s) 760, 1081
BstDEI CTNAG 7 cut(s) 33, 201, 297, 768, 1050, 1121, 1175
BstDSI CCRYGG 1 cut(s) 144
BstENI CCTNNNNNAGG 2 cut(s) 204, 1168
BstF5I GGATG 4 cut(s) 362, 741, 1045, 1071
BstFNI CGCG 1 cut(s) 1168
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 4 cut(s) 256, 361, 417, 608
BstMAI GTCTC 3 cut(s) 642, 947, 1124
BstMBI GATC 4 cut(s) 253, 358, 414, 605
BstMWI GCNNNNNNNGC 2 cut(s) 155, 574
BstNI CCWGG 4 cut(s) 681, 899, 1023, 1086
BstNSI RCATGY 2 cut(s) 164, 879
BstPAI GACNNNNGTC 2 cut(s) 654, 761
BstSCI CCNGG 4 cut(s) 679, 897, 1021, 1084
BstSFI CTRYAG 1 cut(s) 460
BstSLI GKGCMC 1 cut(s) 830
BstUI CGCG 1 cut(s) 1168
BstV1I GCAGC 2 cut(s) 309, 566
BstX2I RGATCY 2 cut(s) 253, 358
BstYI RGATCY 2 cut(s) 253, 358
Bsu36I CCTNAGG 1 cut(s) 768
BsuI GTATCC 1 cut(s) 1163
BsuRI GGCC 2 cut(s) 1140, 1163
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 4 cut(s) 362, 741, 1045, 1071
BtsIMutI CAGTG 2 cut(s) 305, 735
BveI ACCTGC 1 cut(s) 398
CaiI CAGNNNCTG 1 cut(s) 1091
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 2 cut(s) 197, 1100
Csp6I GTAC 1 cut(s) 70
CviAII CATG 5 cut(s) 145, 161, 454, 876, 928
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 7 cut(s) 33, 201, 297, 768, 1050, 1121, 1175
DpnI GATC 4 cut(s) 255, 360, 416, 607
DpnII GATC 4 cut(s) 253, 358, 414, 605
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 1138
Ecl136II GAGCTC 2 cut(s) 52, 775
Eco130I CCWWGG 2 cut(s) 94, 144
Eco147I AGGCCT 1 cut(s) 1163
Eco24I GRGCYC 2 cut(s) 54, 777
Eco47I GGWCC 2 cut(s) 197, 1100
Eco53kI GAGCTC 2 cut(s) 52, 775
Eco57I CTGAAG 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 768
EcoICRI GAGCTC 2 cut(s) 52, 775
EcoNI CCTNNNNNAGG 2 cut(s) 204, 1168
EcoRII CCWGG 4 cut(s) 679, 897, 1021, 1084
EcoT14I CCWWGG 2 cut(s) 94, 144
EcoT38I GRGCYC 2 cut(s) 54, 777
ErhI CCWWGG 2 cut(s) 94, 144
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 5 cut(s) 148, 164, 457, 879, 931
FaqI GGGAC 1 cut(s) 749
FatI CATG 5 cut(s) 144, 160, 453, 875, 927
Fnu4HI GCNGC 2 cut(s) 323, 555
FokI GGATG 4 cut(s) 349, 728, 1052, 1058
FriOI GRGCYC 2 cut(s) 54, 777
Fsp4HI GCNGC 2 cut(s) 323, 555
FspBI CTAG 1 cut(s) 749
GlaI GCGC 1 cut(s) 295
GluI GCNGC 2 cut(s) 323, 555
GsaI CCCAGC 2 cut(s) 432, 732
GsuI CTGGAG 1 cut(s) 870
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 2 cut(s) 1140, 1163
HhaI GCGC 1 cut(s) 296
Hin1II CATG 5 cut(s) 148, 164, 457, 879, 931
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 3 cut(s) 383, 623, 1054
HphI GGTGA 3 cut(s) 116, 422, 1121
Hpy166II GTNNAC 3 cut(s) 735, 828, 919
Hpy188I TCNGA 6 cut(s) 289, 486, 647, 1124, 1157, 1178
Hpy188III TCNNGA 5 cut(s) 32, 504, 952, 1058, 1092
Hpy8I GTNNAC 3 cut(s) 735, 828, 919
HpyAV CCTTC 3 cut(s) 220, 326, 1106
HpyCH4III ACNGT 2 cut(s) 760, 1081
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 574
HpyF3I CTNAG 7 cut(s) 33, 201, 297, 768, 1050, 1121, 1175
Hsp92II CATG 5 cut(s) 148, 164, 457, 879, 931
HspAI GCGC 1 cut(s) 294
Kzo9I GATC 4 cut(s) 253, 358, 414, 605
LmnI GCTCC 5 cut(s) 57, 441, 772, 842, 1003
Lsp1109I GCAGC 2 cut(s) 309, 566
LweI GCATC 3 cut(s) 67, 750, 854
MaeI CTAG 1 cut(s) 749
MaeIII GTNAC 1 cut(s) 340
MalI GATC 4 cut(s) 255, 360, 416, 607
MboI GATC 4 cut(s) 253, 358, 414, 605
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 2 cut(s) 253, 358
MhlI GDGCHC 3 cut(s) 54, 777, 830
MlsI TGGCCA 1 cut(s) 1140
MluNI TGGCCA 1 cut(s) 1140
MlyI GAGTC 2 cut(s) 377, 617
MmeI TCCRAC 3 cut(s) 79, 566, 734
MnlI CCTC 8 cut(s) 148, 426, 477, 669, 685, 763, 1153, 1174
Mox20I TGGCCA 1 cut(s) 1140
MroXI GAANNNNTTC 1 cut(s) 1129
MscI TGGCCA 1 cut(s) 1140
MseI TTAA 1 cut(s) 837
MslI CAYNNNNRTG 1 cut(s) 1010
Msp20I TGGCCA 1 cut(s) 1140
MspA1I CMGCKG 2 cut(s) 322, 1106
MspR9I CCNGG 4 cut(s) 681, 899, 1023, 1086
MunI CAATTG 1 cut(s) 231
Mva1269I GAATGC 3 cut(s) 242, 907, 940
MvaI CCWGG 4 cut(s) 681, 899, 1023, 1086
MvnI CGCG 1 cut(s) 1168
MwoI GCNNNNNNNGC 2 cut(s) 155, 574
NcoI CCATGG 1 cut(s) 144
NdeII GATC 4 cut(s) 253, 358, 414, 605
NlaIII CATG 5 cut(s) 148, 164, 457, 879, 931
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 2 cut(s) 164, 879
PaqCI CACCTGC 1 cut(s) 398
PceI AGGCCT 1 cut(s) 1163
PctI GAATGC 3 cut(s) 242, 907, 940
PdmI GAANNNNTTC 1 cut(s) 1129
PfeI GAWTC 1 cut(s) 1054
PflMI CCANNNNNTGG 1 cut(s) 1091
PkrI GCNGC 2 cut(s) 324, 556
PleI GAGTC 2 cut(s) 377, 617
PpsI GAGTC 2 cut(s) 377, 617
PshAI GACNNNNGTC 2 cut(s) 654, 761
Psp124BI GAGCTC 2 cut(s) 54, 777
Psp6I CCWGG 4 cut(s) 679, 897, 1021, 1084
PspFI CCCAGC 2 cut(s) 428, 728
PspGI CCWGG 4 cut(s) 679, 897, 1021, 1084
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 2 cut(s) 197, 1100
PstI CTGCAG 1 cut(s) 464
PstNI CAGNNNCTG 1 cut(s) 1091
PsuI RGATCY 2 cut(s) 253, 358
PvuII CAGCTG 2 cut(s) 322, 1106
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
RseI CAYNNNNRTG 1 cut(s) 1010
SacI GAGCTC 2 cut(s) 54, 777
SaqAI TTAA 1 cut(s) 837
SatI GCNGC 2 cut(s) 323, 555
Sau3AI GATC 4 cut(s) 253, 358, 414, 605
Sau96I GGNCC 2 cut(s) 197, 1100
SchI GAGTC 2 cut(s) 377, 617
ScrFI CCNGG 4 cut(s) 681, 899, 1023, 1086
SduI GDGCHC 3 cut(s) 54, 777, 830
SfaNI GCATC 3 cut(s) 67, 750, 854
SfcI CTRYAG 1 cut(s) 460
SinI GGWCC 2 cut(s) 197, 1100
SmiMI CAYNNNNRTG 1 cut(s) 1010
SmlI CTYRAG 4 cut(s) 47, 385, 858, 1027
SmoI CTYRAG 4 cut(s) 47, 385, 858, 1027
SseBI AGGCCT 1 cut(s) 1163
SsiI CCGC 3 cut(s) 13, 119, 223
SspI AATATT 1 cut(s) 853
SspMI CTAG 1 cut(s) 749
SstI GAGCTC 2 cut(s) 54, 777
StuI AGGCCT 1 cut(s) 1163
StyD4I CCNGG 4 cut(s) 679, 897, 1021, 1084
StyI CCWWGG 2 cut(s) 94, 144
TaaI ACNGT 2 cut(s) 760, 1081
TaqI TCGA 1 cut(s) 951
TaqII GACCGA 1 cut(s) 651
TfiI GAWTC 1 cut(s) 1054
Tru1I TTAA 1 cut(s) 837
Tru9I TTAA 1 cut(s) 837
TscAI CASTG 2 cut(s) 305, 742
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 322, 554
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 4 cut(s) 438, 470, 624, 699
TspGWI ACGGA 2 cut(s) 287, 734
TspRI CASTG 2 cut(s) 305, 742
Van91I CCANNNNNTGG 1 cut(s) 1091
VneI GTGCAC 1 cut(s) 826
VpaK11BI GGWCC 2 cut(s) 197, 1100
XagI CCTNNNNNAGG 2 cut(s) 204, 1168
XapI RAATTY 4 cut(s) 276, 675, 705, 722
XceI RCATGY 2 cut(s) 164, 879
XmnI GAANNNNTTC 1 cut(s) 1129
XspI CTAG 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.