RchiOBHm_Chr7g0242671

Cytochrome c oxidase subunit 6b-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004101
Physical Location & Seq
Reverse (-)
68867703 .. 68871195
3493 bp
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UTR
Exon/CDS
Intron
PRQ21748

Sequence Viewer

Length: 591 bp
ATGGCGGAGCCGCAAGCCGACAAACCCCAAACCCTAGCTGAGCAATACGCATTGGAGAAACCAGATGTGGTAACCAAGCCTGTTGAAGTAAAAGTGGTTGAGACCCCGGACAATGTACCTTCCAAGGAAGTTGTCTCTGAGAAAGCTGAAGAAGCGCCTGCTGCTCCAGAAGAAAGTCCAGTAGTTACACCAGCTAGTGAAGAAACCAGTGAAGTCAGTCCTACTGTCGCTGAAACTGAAACAAAACCTGCCTCTGAGAACAGCAGTGAAGCAGCTCCTGAAGAAAGCAGTGAAGCAGCTCCTGAAGAAAGCAGTGATAGTACTGAAACATCTGTTGACCAAGAAGCAGTTGAAGAGACACCTGAAATTAAGATTGAGACAGCACCAGCAGATTTCCGTTTCCCAACTACAAATCAGACCAGGCATTGCTTTACCCGATACGTAGAATATCATCGGTGCATAGCTGCAAAGGGGGAAGAAGCGCCAGAGTGTGATAAATTTAAAAAGTACTATCGTTCTCTTTGCCCCGGTGAATGGGTGGATAGATGGAATGAGCAAAGGGAGAACGGAACCTTTCCAGGTCCTCTGTAG

Protein Analysis

196

Amino Acids

21.65

Weight (kDa)

4.34

Isoelectric Point (pI)

68.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX6B PF02297 133 - 193 7.6e-22 Cytochrome oxidase c subunit VIb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 256
AciI CCGC 2 cut(s) 5, 11
AcsI RAATTY 1 cut(s) 497
AcuI CTGAAG 3 cut(s) 168, 300, 324
AfaI GTAC 3 cut(s) 117, 322, 509
AfiI CCNNNNNNNGG 1 cut(s) 534
AgsI TTSAA 2 cut(s) 86, 353
AjnI CCWGG 2 cut(s) 419, 577
AluBI AGCT 6 cut(s) 38, 146, 194, 275, 299, 464
AluI AGCT 6 cut(s) 38, 146, 194, 275, 299, 464
Alw26I GTCTC 4 cut(s) 95, 139, 350, 371
AlwNI CAGNNNCTG 2 cut(s) 278, 302
ApeKI GCWGC 4 cut(s) 161, 272, 296, 464
ApoI RAATTY 1 cut(s) 497
AspLEI GCGC 2 cut(s) 157, 484
AspS9I GGNCC 1 cut(s) 581
AsuC2I CCSGG 2 cut(s) 107, 528
AsuHPI GGTGA 1 cut(s) 542
AvaII GGWCC 1 cut(s) 581
BbvI GCAGC 4 cut(s) 148, 284, 308, 451
BccI CCATC 1 cut(s) 540
BciT130I CCWGG 2 cut(s) 421, 579
BcnI CCSGG 2 cut(s) 107, 528
BcoDI GTCTC 4 cut(s) 95, 139, 350, 371
BfaI CTAG 2 cut(s) 35, 195
BfmI CTRYAG 1 cut(s) 587
BfoI RGCGCY 2 cut(s) 158, 485
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 5 cut(s) 11, 162, 273, 297, 465
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 5 cut(s) 12, 163, 274, 298, 466
BmcAI AGTACT 2 cut(s) 322, 509
Bme1390I CCNGG 4 cut(s) 107, 421, 528, 579
Bme18I GGWCC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 2 cut(s) 9, 571
BmrFI CCNGG 4 cut(s) 107, 421, 528, 579
BpmI CTGGAG 1 cut(s) 150
Bpu1102I GCTNAGC 1 cut(s) 39
BpuMI CCSGG 2 cut(s) 107, 528
BsaAI YACGTR 1 cut(s) 442
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 105, 123, 526
Bsc4I CCNNNNNNNGG 1 cut(s) 534
Bse1I ACTGG 2 cut(s) 179, 207
Bse3DI GCAATG 1 cut(s) 424
BseBI CCWGG 2 cut(s) 421, 579
BseDI CCNNGG 3 cut(s) 105, 123, 526
BseLI CCNNNNNNNGG 1 cut(s) 534
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 3 cut(s) 30, 129, 246
BseNI ACTGG 2 cut(s) 179, 207
BseXI GCAGC 4 cut(s) 148, 284, 308, 451
BsiSI CCGG 2 cut(s) 107, 528
BslI CCNNNNNNNGG 1 cut(s) 534
BsmAI GTCTC 4 cut(s) 95, 139, 350, 371
Bso31I GGTCTC 1 cut(s) 95
Bsp1720I GCTNAGC 1 cut(s) 39
BspACI CCGC 2 cut(s) 5, 11
BspCNI CTCAG 3 cut(s) 31, 130, 247
BspLI GGNNCC 2 cut(s) 9, 571
BspMI ACCTGC 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 424
BsrI ACTGG 2 cut(s) 179, 207
BssECI CCNNGG 3 cut(s) 105, 123, 526
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 421, 579
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 1 cut(s) 348
BstBAI YACGTR 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 15, 159
BstDEI CTNAG 3 cut(s) 39, 138, 255
BstEII GGTNACC 1 cut(s) 70
BstH2I RGCGCY 2 cut(s) 158, 485
BstHHI GCGC 2 cut(s) 157, 484
BstMAI GTCTC 4 cut(s) 95, 139, 350, 371
BstMWI GCNNNNNNNGC 2 cut(s) 152, 161
BstNI CCWGG 2 cut(s) 421, 579
BstPI GGTNACC 1 cut(s) 70
BstSCI CCNGG 4 cut(s) 105, 419, 526, 577
BstSFI CTRYAG 1 cut(s) 587
BstSNI TACGTA 1 cut(s) 442
BstV1I GCAGC 4 cut(s) 148, 284, 308, 451
BtsI GCAGTG 3 cut(s) 271, 295, 319
BtsIMutI CAGTG 4 cut(s) 214, 271, 295, 319
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 15, 159
CaiI CAGNNNCTG 2 cut(s) 278, 302
CfoI GCGC 2 cut(s) 157, 484
Cfr13I GGNCC 1 cut(s) 581
Csp6I GTAC 3 cut(s) 116, 321, 508
CviJI RGCY 9 cut(s) 10, 17, 38, 79, 146, 194, 275, 299, 464
CviKI_1 RGCY 9 cut(s) 10, 17, 38, 79, 146, 194, 275, 299, 464
CviQI GTAC 3 cut(s) 116, 321, 508
DdeI CTNAG 3 cut(s) 39, 138, 255
DraI TTTAAA 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 442
Eco130I CCWWGG 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 581
Eco57I CTGAAG 3 cut(s) 168, 300, 324
Eco91I GGTNACC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 581
EcoO65I GGTNACC 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 419, 577
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaiI YATR 1 cut(s) 461
Fnu4HI GCNGC 5 cut(s) 11, 162, 273, 297, 465
Fsp4HI GCNGC 5 cut(s) 11, 162, 273, 297, 465
FspBI CTAG 2 cut(s) 35, 195
GlaI GCGC 2 cut(s) 156, 483
GluI GCNGC 5 cut(s) 11, 162, 273, 297, 465
GsuI CTGGAG 1 cut(s) 150
HaeII RGCGCY 2 cut(s) 158, 485
HapII CCGG 2 cut(s) 107, 528
HhaI GCGC 2 cut(s) 157, 484
Hin6I GCGC 2 cut(s) 155, 482
HinP1I GCGC 2 cut(s) 155, 482
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HpaII CCGG 2 cut(s) 107, 528
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 3 cut(s) 139, 256, 417
Hpy188III TCNNGA 3 cut(s) 167, 278, 302
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 2 cut(s) 459, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 161
HpyF3I CTNAG 3 cut(s) 39, 138, 255
HpySE526I ACGT 1 cut(s) 441
HspAI GCGC 2 cut(s) 155, 482
LmnI GCTCC 4 cut(s) 7, 169, 280, 304
Lsp1109I GCAGC 4 cut(s) 148, 284, 308, 451
MaeI CTAG 2 cut(s) 35, 195
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 2 cut(s) 70, 184
MboII GAAGA 7 cut(s) 161, 182, 212, 293, 317, 365, 488
MluCI AATT 2 cut(s) 366, 497
MnlI CCTC 1 cut(s) 262
MseI TTAA 2 cut(s) 369, 501
MspI CCGG 2 cut(s) 107, 528
MspR9I CCNGG 4 cut(s) 107, 421, 528, 579
MvaI CCWGG 2 cut(s) 421, 579
MwoI GCNNNNNNNGC 2 cut(s) 152, 161
NciI CCSGG 2 cut(s) 107, 528
NlaIV GGNNCC 2 cut(s) 9, 571
PkrI GCNGC 5 cut(s) 12, 163, 274, 298, 466
Ppu21I YACGTR 1 cut(s) 442
PpuMI RGGWCCY 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 419, 577
PspEI GGTNACC 1 cut(s) 70
PspGI CCWGG 2 cut(s) 419, 577
PspN4I GGNNCC 2 cut(s) 9, 571
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PstNI CAGNNNCTG 2 cut(s) 278, 302
RsaI GTAC 3 cut(s) 117, 322, 509
RsaNI GTAC 3 cut(s) 116, 321, 508
SaqAI TTAA 2 cut(s) 369, 501
SatI GCNGC 5 cut(s) 11, 162, 273, 297, 465
Sau96I GGNCC 1 cut(s) 581
ScaI AGTACT 2 cut(s) 322, 509
ScrFI CCNGG 4 cut(s) 107, 421, 528, 579
SfcI CTRYAG 1 cut(s) 587
SinI GGWCC 1 cut(s) 581
SnaBI TACGTA 1 cut(s) 442
Sse9I AATT 2 cut(s) 366, 497
SsiI CCGC 2 cut(s) 5, 11
SspMI CTAG 2 cut(s) 35, 195
StyD4I CCNGG 4 cut(s) 105, 419, 526, 577
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 226
TaiI ACGT 1 cut(s) 444
TasI AATT 2 cut(s) 366, 497
TatI WGTACW 2 cut(s) 320, 507
TauI GCSGC 1 cut(s) 13
Tru1I TTAA 2 cut(s) 369, 501
Tru9I TTAA 2 cut(s) 369, 501
TscAI CASTG 4 cut(s) 214, 271, 295, 319
TseI GCWGC 4 cut(s) 161, 272, 296, 464
TspGWI ACGGA 2 cut(s) 386, 582
TspRI CASTG 4 cut(s) 214, 271, 295, 319
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 1 cut(s) 497
XspI CTAG 2 cut(s) 35, 195
ZrmI AGTACT 2 cut(s) 322, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.