Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002032

Physical Location & Seq

Genomic Coordinates Reverse (-)

51506501 .. 51527508

Gene Structure 21008 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003679

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7833 bp

ATGGCCGCAAACACCGCCTCCGTCCAACCGGGTCGGTTTCGGGTTCTAAAGCAAGGGGCTAAACCGGTCGACCCGAATCCGGGTCCCGTGGTTTACTGGATGTTCAGAGACCAACGGGTCAAAGACAACTGGGCGTTGATCCACGCCGTTGAAGAGGCCAACAAGGCCCATGTCCCCGTCGCCGTCGCGTTCAATTTGTTCGATCAGTTTCTTGGCGCCAAAGCCCGCCATTTAGGGTTTATGCTTAGGGGCTTGCGACAATTACATCCTGATCTTCAAGAAACGCATCAGATTCCATTTTTTCTTGTCCGGGGAGAAGCGGAGGAGACTATACCCAATTTTGTGAGGGAATGCGGTGCCTCACTTTTAGTAACAGATTTTTCGCCTTTAAGGGAGGTTCAGAAATGTAAAGAGGAAATATGCAGGAGGGTTAGTGATTTTGTGACCGTACATGAAGTTGATGCCCACAATGTGGTGCCCGTTTGGGCGGCATCGGAGAAGTTGGAGTATAGTGCTAGGACCATAAGGAGTAAGATAAATAAAAGGCTGCCTGAGTATCTTATTGAATTTCCTACGCTGCAGCCATCGATTGCAAAATGGGTGGGCGCAAGTCCGGCGATTGATTGGGATGGTCTTATTGATGAAGTTTTAAGGAAAGGAGCAGAAGTTGCTGAAGTGGACTGGTGCGAACCAGGAGAAAAGGCAGCAATGGAAGTTTTGATGGGTAGCAAAAATGGATTCTTGACAAAAAGGTTGAAGGGTTATTCATCAGACCGGAATAACCCTTTGAAACCAAGAGGGGTTTCTGGTCTTTCTCCATATTTGCATTTTGGGCAAATATCTGCACAGAGGTGTGCCTTGGAGGCACGCAGCGTCAGGAAACTCAGTCCCCTGGCAGTGGATGCATTTTTAGAGGAGTTGATTGTGCGCAGAGAACTTGCTGACAACTACTGCTTCTACCAGCCTCAATATGATTCATTGCAAGGGGCATGGGAATGGGCACGTAAGACCTTAATGGACCATGCTTCAGATAAGCGAGAACATATTTACACGAGGGAGCAATTGGAGAAGGCACAAACAGCTGATCCTCTCTGGAATGCCTCCCAATTGGAGATGGTTCATTACGGGAAGATGCATGGTTTTATGCGGATGTATTGGGCCAAAAAGATCCTTGAGTGGACAAGAGGACCTGAAGAAGCCCTTGAAATATCCATATATTTAAATAACAAGTATGAAATAGATGGAAGGGATCCGAATGGATATGTTGGTTGCATGTGGTCAATATGCGGTGTTCACGACCAGGGTTGGAAGGAACGACCAATTTTTGGGAAAATACGTTATATGAATCATGCAGGCTGCAAACGGAAATTTGACGTGGATGGATACATTTCCTATGTGAAGAGGCTTGGTGCAAGCAAGAAGAGAAAAGTGGAAAGTCTCCTACTCGGCAAAAAGGCCGAGGAACTCTGCAATTGGCATACAGACATGGAGATTGCTACCGGAGCTGCTACAGATCTTGTTGGAAAGATTTATTCAACAGTTGGACGGCAAGTGGGTTATTTAATCCACTACAAACGCAACCGTGATGATCTAAAGACTGAAGTGGGCAAATTAAGTGTTGCAAAAGCAAAAGTGAGTGAGAAGATTAACGACGCTGAAGGCAGGGGTGAAATGATTCATGCGCATATCCTCGACTGGCTGAATAATGTGGCTACAATTACTAAGGAGGCAGGCAAACTGTTGAAAGAGAAGCAACAAGCAACTATTGAAGATGCAAACAATTCCCAGCCAAAGACGAAGTGTGTCCATGGGTTTTGTCCAAATCTGATGCTCCTCCGTAGTCGAAGCAAGAGCTTGTTGGCATTGATTGAGAGTGTTACCGAGCTGATCAACAAAGAAAAAGAACTTTCTGACATTTCATATGTTCCCCCTAAAGATCTACGGACTGAAGTGAACGAATTAATAGTTGCTAAAGACACAGTAGCTGAGAACGTCCGTGAAGCTGAAGCGAAGGGTGAAAAAATCCATGCAGATGTCCAAGAGTGGCTGAAAAGCGTGGCGGAGATTGTTAAAATGGTAGAAGAATTCTTGAAAGACAAGCAACAATCAGTTGAAGAGACAAGCGAATTTATGAAAGATGAAATCCAAGCAGCTGAAAAGCCAACCACCTCCTTAAGTCATGGAAGCAAGGAATTGGAATTGCTGCAGAGGGTTGCTGAACTCTACGAGATGAGAGAATTTTCTGATATTTCGTACGTCCCCCCTAAAGATCTACAGACTGAAGTGGGCAAATTAAGAGATTCTAAAGACACGGTAGCTGAGAACGTCAGTGAAGCTGAAGCGAAGGGTGAAAAAATCCATGCAAATGTCCAAAGCTGGCTGAAAAGAGTGGTTGATATTGTTAAAGAGGTAGAAGAATTCTTGAAGGACAAGCACCAATCAATTGAAGAGACAAGCGAATTTATGAAAGATGAAATCCAAGCAATTGAAAAGCCAAACACCTCCTTAAGTCATGGAAGCAAGGAATTGGAATTGCTGCAGGGGGTTGCTGAACTCTACGAGATGAGAGAATTTTCTAAAATTTCGTATGTTCTTCCGGCAGAAGATGTATGTATGGCATCTGAGGAAGACTATGAGAAATTTGATTCAAGAACTTCAATACTGGAGGAAATCTTGAAGGAATTGGGAACTTCTAGCTCTGAAATGATATTGGTGTATGGTATTGGCGGTGTGGGAAAGACCACGCTCGTTGAAGAAGTTCTAAGGAAAACTCAAAAAGAGAAATTCTTTGAGGATGCAGTAATGGTACGGGATGTGAAAATTCCAAACTTGGAAGCAATTCAAAATCAAATTGCTGACAAGTTACGTCTGGACATTTCAAAAATTAAGACTCTACCTGGAAGAGCCGATAAAATAAATGAAAGAATCAAGGACAAGAAGACTCTTGTCATTTTTGATGATGTTTGGGGAGAACTTGATTTGAAGGCTGTGGGACTTCCCCACAAATCCACTTGTAGAATATTGTTGACATCCAGAAGTCAACAAGTATTTTCTCATAAGATTAAGCAAAAAGAGTTTCTGCTTGGGGTTTTAGATGAAAAAGAAGCATGGGCTTTATTTGAGAAGAAAGCAGGTGATGTTGTTGCAGATCCTACCATTCAAAATGTAGCAACTGAAGTAGCCAAAAAATGTGGAGGGTTGCCTGTTTTTATTATCACTGTTGCAAGAGCTTTAGGAAACAAGACTCTACCCATCTGGAAAAATGCCTTGAGATGCCTCGAAAGGTTTGATGAGAAAGGAGACTTAACTGAAAAGGCATACTTGGGTATCGAGTGGAGTTACAAGCAATTGAGTGTCAAGGATCAGCTTCAGCCATTGTTCTTGTTGTGTGGTCTTATTGCAAAGGGCAACTATGTTTCATTTACAGACTTGTTGAAATATAGTATAGGTTTGGGTTTGTTTGAAGACTGTACTGTGGAAGACGCACGAAATGCCTTGTATCAACAGGTTGACAAGCTTAAAAGTTCTTGTCTATTGCTAGACGATGATGATGGTACATGTGTCAGAATGCACGATCTTGTGCATGATGTTGCTAACAGGATTGCAAACAGAGATAATCATGTGTTGGTTGAAGATGGAGGTCATGAGAGGAAAAAATGGCCAAATGAAGATTTCTTTACTGAGTGCAGTAAGATCTGTTTGCCTCGTTGCAATATCGCCACCCTTCCTCAAGTACCTTGGAAATGCCCAAAATTGGAGATGTTTCATTTGCGCGGAGAAGATGATTATGACAGTGAAGATTATGATTATGACAGTGAAGATGATTATGACAGGGAAGATGATGATGATTCCTCGCCAAAATTGGAGATGCTTCATTTGCGCGGAGAAGATGATTATGACAGGGAAGATGATGATGATTCCTCGCCTAATATACCAAGCAACTTTTTCGTGGAGATGAAAAACCTCAAAGTTTTGGGTTTAAGCGAGGTGCCTCTTCTTCCACTACCCTCATCTTTTTCGTTTTTAAAGAATCTCCAAACATTGTGTTTAGATGGATGCATGTTTGAAGACATTGCTCTAGTTGGAGAGCTAAGAAACTTGGAAGTTCTTAGCCTTGTAAAATCCAAGGTTAAACAATTACCCAAAGAAATTGGGCAGTTAACCCGTCTGCGGTTGGTGGATTTGTCTGACTCTTCACTTATAGTGATTCCAGCTGGTGTCATTTCAAATTGGACAAGACTTGAAGAGTTGAGAATGAGGAACATTTTTATGATTTGGAAGAGTAGAGAAGTCATCTGTGAAACAAGTAATGCTAGCCTTTTGGAGCTGATGAACTTGCCCAAGCTAACCGCATTAGAAGTACACATTCCAGATCATGACATTCTTCCAGCGAAGTTTTTCTCTGACAGGTTGGAAAAATTCCAAATATTTATTGAGCCGACGACGTGGGCGCCGCGGCGTATGTATGAAACAACCCTCAACAAGCTTGTACTAGAGCTCACTACCAGATATGAATTGGACCAAGGTCTAAAAGTATTGCTGAAGAGATGTGGAGAATTATACTTTATTGGGACGAAAGATGTTGATTTTTCATTTGTCATGAAGACAGAGGTTGTGATGTCCAGCTTAACAACTTTGGAGGTGACAAATTGTGATGGTTTAAAATACTTGTTATCATCTTCTATGGCTAGAAGTCTCGTGAAACTCAAAAAGCTCAAGATATCAAATTGTAAGGCCATGGAAGAAATAGTATCAACAAGAGAATCCCAAAATAACATAATTGATAAGCTAGAAATCCTGGAACTGAACATACTTCCAAACCTTGCTAGATTCTGCACAGCTGGAAGTTGTATTGAATTTCCATCATTGGAGACTCTGTATATACAAGCCTGTGGAAAACTAGGAGAAAGTACTGCAAAAGACACTCCATTCCGCAGAGAGGTTCAAGAGAGAGAGTTGGATGAGAACTTTCAGGCCCACAATGAGACTTCTAGCCAAAACTTTCTTTTTGATGAAAAGATAAGATCTCCAAGACTGGAGATATTGCAAATCATCAATGACGACGGCGGGGACTATGGAAATAGGTTCTTGCAGACCATATGGCACAACCAATTGGATCCGGGCTCATTTTGCAGACTCAGAGAAGTCTATGTGGACAATTGTGTCAGTCTAAGAAGCATTTTTCCACCCAGTATGATGGGAAGATTGAGTGCTTTAGAAACCTTAGACATAAATGATTGCGAGTCCATGGAAGTGGTATTTGATGAACGTAGAGAAGCTAATGTCAGCGAAACACATGGCACATCAACCGCTCAGTTGAAAGACGGTCAAAATCTAAAGTCAGTGGATATATACAATTGTCACAGATTGAAATACATTTTTCCCGCATCTATGGTAACTAGAGGTCTTCAACAGCTGCGAATGATGACAGCGGGTTGTTGTGATGAAATGGAGAGTGTTGTTGCCAAGGAAGAGGGGACTCTAGAATCAACAAGGCATCCCAACTTTGTGCTCCCAAAAATTGAATCTATGATTTTTGAATCTATGCAAAGGCTTAAGAGCTTCTACCCCGGAATGTATATTTCTGAGTGGCCCTTGCTCAAACATTTCAAGTTTGAAGGCGAAGTGGAGATTTTTGCTGCAGAACTATCAGACTTTCAGGAAAGGCAAATGCATGCTCCAACTAAACAACCTTTCTTGCTAATTGATAAGGGTTCATTCCCCGTCTTGGAAACATTGGAGGTGTCTATCGCGGAGATTTTGTATGGTCCATCTTCATCTTCATCTTCATCCCCAGTAGAGTCGATCTTTAGCAAACTGAAATCTATTCATTTTTATTGGTGTGAACCCTTCTCCAAGCATGCGGTGGTTGTGTCCAGCTTAACAACTTTGGAGGTGACAAATTGTGATGGTTTAAAATACTTGTTATCATCTTCTATGGCTAGAAGTCTCGTGAAACTCAAAAAGCTCAAGATATCAAATTGTAAGGCCATGGAAGAAATAGTATCAACAAGAGAATCCCAAAATAACATAATTGATAAGCTAGAAATCCTGGAACTGAACTTACTTCCAAACCTTGCTAGATTCTGCACAGCTGGAAGTTGTATTGAATTTCCATCATTGGAGACTCTGTCTATGCGAGAATGTAGAAAACTAGGAGAAAGTACTGCAAAAGACACTCCATTCCGCAGAGAGGTTCAAGAGAGAGAGTTGGATGAGAACTTTCAGGCCCACAATGAGACTCCTAGCCAAAACTTTCTTTTTGATGAAAAGATAAGATCTCCAAGACTGGAGATATTGAAAATCATCAGTTACTACGGCAGGGACTATGGAAATAGGTTCTTGCAGACCATATGGCACAACCAATTGGATCCGGGCTCATTTTGCAGACTCAGAGAAGTCTATGTGGAAAATTGTGTCAGTCTAAGAAGCATTTTTCCACCTAGTATGATGGGAAGATTGAGTGCTTTAGAAACCTTAGACATAAAAGGTTGCGAGTCCATGGAAGTGGTATTTGATGAACGTAGAGAAGCTAATGTCAGCGAAACACATGGCACATCAACCGCTCAGTTGAAAGACGGTCAAAATCTAAAGTCAGTGGTTATATACAATTGTTACAGATTGAAATACATTTTTCCCGCATCTATGGTAACTAGAGGTCTTCAACAGCTGCGAAAGATGAAAGCGGGTCATTGTGATGAAATGGAGAGTGTTGTTGCCAAGGAAGAGGGGACTCTAGAATCAACAAGGCATCCCAACTTTGTGCTCCCAAAAATTGAATCTATGATTTTTGAATCTACGCAAAGGCTTAAGAGCTTCTACCCCGGAATGTATATTTCTGAGTGGCCCTTGCTCAAACATTTCAAGTTTGAAGGCGAAGTGGAGATTTTTGCTGCAGAACTATCAGACTTTCAGGAAAGGCAAATGCATGCTCCAACTAAACAACCTTTCTTGCTAATTGATAAGGGTTCATTTCCCGTCTTGGAAAAATTGGAGGTGTCTACCGCGGAGATTTTGTATGGTCCATCTTCATCCCCAGTAGAGTCGATCTTTAGCAGACTAAAATATATACATATTTGGAGGTGTAAACCCTGCCTCGAGCATGCGGCAGTGCTTTTCCTTCAGAAATTTCCTTATCTGGAAGTCCTAAAATTGTACAGGACTATGCCCAAAATATTTGTGGACGAAAGAAATGGTTGTGGTGAAACTGTAAGAGTTGGGTCCCTCCCACGTGTAAGAGTTCTAGAGCTCTACGATGTGGATGTGGGGCATCTTGGGATAGAGAACCAACCAGCGGGCCCAGTTTTTCCAAACTTGGAATCTCTTACCGTGATCGCATGTCACAGACTAGAGAATTTAGTGTCATCTGCAGTATCCTTCCAGAATCTAACAAAGTTGGAAGTATCTAACTGTGATGGATTGAAGTGTTTAGCCCCTTCTTCGGTAGCTAACAGCTTAGTGCAACTCACGAATTTGAAAGTGACACATTGTAAAGAAATTGTAGTATTAGTGGCAGGCAACGAAGATGAAGATGATGATACAGAAAATGAGATTGCTTTCAGCCGGTTACAAAATTTGAAACTTTGCTGGCTACCCAGTCTACAGGGCTTTTGCTCCCGAAATTGCACTGCCAAACTCCCATTGTCAATCACTGTAGATGTAGACTACTGCCCGATTCAGTTGAAGATTTCCCCCGATGGGGTACCAATAGCAGAAAGCGATGGAGAAATGAAGATGCGGTTGGAGATGGAGATAGAAGATACAGCTGACGATGACCACTCAACTGGAGCCTTGTTGAATTTGGATTTCTATTTGGCTATTCCGTTTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000719 GO:0003674 GO:0003824 GO:0003904 GO:0003913 GO:0005488 GO:0006139 GO:0006259 GO:0006281 GO:0006290 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009650 GO:0009987 GO:0016829 GO:0016830 GO:0033554 GO:0034641 GO:0036094 GO:0043167 GO:0043168 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051716 GO:0071704 GO:0071949 GO:0090304 GO:0097159 GO:0140097 GO:1901265 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_photolyase

Protein Analysis

2611

Amino Acids

297.1

Weight (kDa)

5.45

Isoelectric Point (pI)

43.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_photolyase	PF00875	30 - 192	1.8e-29	DNA photolyase
NB-ARC	PF00931	887 - 1045	3.8e-28	NB-ARC domain
LRR_14	PF23598	1318 - 1496	1.2e-08	Leucine-rich repeat region
LRR_RPS2	PF23247	1538 - 1634	7e-13	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1697 - 1764	1.9e-13	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1943 - 2052	2.9e-13	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	2114 - 2182	4.3e-13	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	2458 - 2542	4.1e-10	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12370 AT1G12370 AT1G12370
fragaria_vesca	FvH4_5g00790 FvH4_5g00790 FvH4_5g00790 FvH4_5g00790 FvH4_5g00790 FvH4_5g00790 FvH4_5g00790
malus_domestica	MD04G1078900.v1.1
prunus_persica	Prupe.5G080300_v2.0.a1 Prupe.5G080300_v2.0.a1 Prupe.5G080300_v2.0.a1 Prupe.5G080300_v2.0.a1
pyrus_communis	pycom04g07070
rosa_chinensis	RchiOBHm_Chr7g0201341 RchiOBHm_Chr7g0201371 RchiOBHm_Chr7g0201391
rosa_laevigata	RLG00000003679
rosa_multiflora	Rmu_co8093880.1_g000001 Rmu_co8322589.1_g000001 Rmu_sc0001469.1_g000010 Rmu_sc0001469.1_g000012 Rmu_sc0013971.1_g000001 Rmu_sc0013971.1_g000004 Rmu_sc0024878.1_g000001 Rmu_sc0024878.1_g000004 Rmu_sc0026181.1_g000001
rosa_roxburghii	Rroxscaffold_3G00226330 Rroxscaffold_3G00255330 Rroxscaffold_3G00255340 Rroxscaffold_3G00255350
rosa_rugosa	Rorug07G0062600 Rorug07G0062700
rosa_samantha	Rh7AG190200 Rh7BG192600 Rh7CG200900 Rh7CG201300 Rh7CG201400 Rh7CG201500 Rh7DG195000 Rh7DG195200
rosa_wichuraiana	Rw7G016680 Rw7G016690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3122
AasI	GACNNNNNNGTC	2 cut(s)	116, 5162
Acc16I	TGCGCA	2 cut(s)	929, 1683
Acc36I	ACCTGC	1 cut(s)	3122
Acc65I	GGTACC	1 cut(s)	7702
AccB1I	GGYRCC	6 cut(s)	215, 356, 475, 3988, 4450, 7702
AccB7I	CCANNNNNTGG	2 cut(s)	472, 1325
AccBSI	CCGCTC	2 cut(s)	5312, 6566
AccI	GTMKAC	4 cut(s)	69, 7001, 7600, 7662
AccII	CGCG	6 cut(s)	188, 3774, 3882, 4456, 5753, 7007
AcoI	YGGCCR	2 cut(s)	3, 3659
AcvI	CACGTG	1 cut(s)	7232
AcyI	GRCGYC	2 cut(s)	216, 4451
AdeI	CACNNNGTG	1 cut(s)	472
AflII	CTTAAG	4 cut(s)	2173, 2506, 5555, 6809
AflIII	ACRYGT	2 cut(s)	3557, 7231
AgeI	ACCGGT	1 cut(s)	64
AjiI	CACGTC	2 cut(s)	1375, 4446
AjnI	CCWGG	6 cut(s)	691, 891, 1299, 2896, 4797, 6051
AjuI	GAANNNNNNNTTGG	4 cut(s)	787, 819, 7724, 7756
AleI	CACNNNNGTG	1 cut(s)	850
Alw21I	GWGCWC	4 cut(s)	4500, 5514, 6768, 7251
AlwNI	CAGNNNCTG	1 cut(s)	1985
Ama87I	CYCGRG	1 cut(s)	7097
ApaI	GGGCCC	1 cut(s)	7301
AseI	ATTAAT	1 cut(s)	1961
AsiGI	ACCGGT	1 cut(s)	64
Asp700I	GAANNNNTTC	4 cut(s)	1674, 2757, 2838, 4397
Asp718I	GGTACC	1 cut(s)	7702
AspLEI	GCGC	7 cut(s)	218, 608, 930, 1684, 3774, 3882, 4453
AsuC2I	CCSGG	7 cut(s)	30, 81, 311, 5121, 5571, 6375, 6825
AsuHPI	GGTGA	7 cut(s)	1679, 2027, 2360, 3146, 4654, 5908, 7214
AsuNHI	GCTAGC	1 cut(s)	4313
AvaI	CYCGRG	1 cut(s)	7097
AvaII	GGWCC	8 cut(s)	83, 519, 1018, 1187, 4519, 5768, 7022, 7221
BaeGI	GKGCMC	3 cut(s)	480, 1003, 7301
BaeI	ACNNNNGTAYC	4 cut(s)	3310, 3343, 3546, 3579
BalI	TGGCCA	1 cut(s)	3661
BamHI	GGATCC	3 cut(s)	1249, 5116, 6370
BanI	GGYRCC	6 cut(s)	215, 356, 475, 3988, 4450, 7702
BanII	GRGCYC	5 cut(s)	4500, 5126, 6380, 7251, 7301
BauI	CACGAG	3 cut(s)	1051, 4697, 5951
BbrPI	CACGTG	1 cut(s)	7232
BbsI	GAAGAC	8 cut(s)	2634, 2945, 3471, 3486, 4074, 4610, 5399, 6653
Bbv12I	GWGCWC	4 cut(s)	4500, 5514, 6768, 7251
BceAI	ACGGC	5 cut(s)	131, 167, 1562, 5080, 6334
BcgI	CGANNNNNNTGC	2 cut(s)	3928, 3962
BciT130I	CCWGG	6 cut(s)	693, 893, 1301, 2898, 4799, 6053
BciVI	GTATCC	2 cut(s)	1376, 7384
BclI	TGATCA	1 cut(s)	1887
BcnI	CCSGG	7 cut(s)	30, 81, 311, 5121, 5571, 6375, 6825
BfoI	RGCGCY	2 cut(s)	219, 4454
BfrI	CTTAAG	4 cut(s)	2173, 2506, 5555, 6809
BfuAI	ACCTGC	1 cut(s)	3122
BfuI	GTATCC	2 cut(s)	1376, 7384
BglI	GCCNNNNNGGC	2 cut(s)	164, 863
BglII	AGATCT	6 cut(s)	1513, 1936, 2269, 3693, 5024, 6278
BmcAI	AGTACT	2 cut(s)	4912, 6166
Bme18I	GGWCC	8 cut(s)	83, 519, 1018, 1187, 4519, 5768, 7022, 7221
BmeT110I	CYCGRG	1 cut(s)	7097
BmgBI	CACGTC	2 cut(s)	1375, 4446
BmrI	ACTGGG	6 cut(s)	139, 5184, 5789, 7031, 7295, 7590
BmtI	GCTAGC	1 cut(s)	4317
BmuI	ACTGGG	6 cut(s)	139, 5184, 5789, 7031, 7295, 7590
BoxI	GACNNNNGTC	2 cut(s)	2945, 4524
BpiI	GAAGAC	8 cut(s)	2634, 2945, 3471, 3486, 4074, 4610, 5399, 6653
BpmI	CTGGAG	4 cut(s)	2684, 5057, 6311, 7806
Bpu10I	CCTNAGC	1 cut(s)	245
BpuEI	CTTGAG	5 cut(s)	1193, 3289, 3714, 4700, 5954
BpuMI	CCSGG	7 cut(s)	30, 81, 311, 5121, 5571, 6375, 6825
Bsa29I	ATCGAT	1 cut(s)	587
BsaAI	YACGTR	2 cut(s)	1004, 7232
BsaHI	GRCGYC	2 cut(s)	216, 4451
BsaI	GGTCTC	1 cut(s)	102
BsaWI	WCCGGW	3 cut(s)	64, 774, 1499
Bse118I	RCCGGY	2 cut(s)	64, 7563
Bse3DI	GCAATG	3 cut(s)	714, 977, 4071
BseBI	CCWGG	6 cut(s)	693, 893, 1301, 2898, 4799, 6053
BseCI	ATCGAT	1 cut(s)	587
BseMI	GCAATG	3 cut(s)	714, 977, 4071
BseRI	GAGGAG	3 cut(s)	338, 929, 1823
BseSI	GKGCMC	3 cut(s)	480, 1003, 7301
BseYI	CCCAGC	1 cut(s)	1785
BsgI	GTGCAG	4 cut(s)	828, 3706, 4819, 6073
Bsh1236I	CGCG	6 cut(s)	188, 3774, 3882, 4456, 5753, 7007
Bsh1285I	CGRYCG	1 cut(s)	69
BshNI	GGYRCC	6 cut(s)	215, 356, 475, 3988, 4450, 7702
BshTI	ACCGGT	1 cut(s)	64
BshVI	ATCGAT	1 cut(s)	587
BsiEI	CGRYCG	1 cut(s)	69
BsiHKAI	GWGCWC	4 cut(s)	4500, 5514, 6768, 7251
BsiHKCI	CYCGRG	1 cut(s)	7097
BsiWI	CGTACG	1 cut(s)	2253
BsmI	GAATGC	3 cut(s)	356, 1102, 3573
Bso31I	GGTCTC	1 cut(s)	102
BsoBI	CYCGRG	1 cut(s)	7097
Bsp120I	GGGCCC	1 cut(s)	7297
Bsp1286I	GDGCHC	9 cut(s)	480, 1003, 4500, 5126, 5514, 6380, 6768, 7251, 7301
Bsp1407I	TGTACA	1 cut(s)	7155
Bsp19I	CCATGG	5 cut(s)	1807, 4737, 5247, 5991, 6501
BspDI	ATCGAT	1 cut(s)	587
BspFNI	CGCG	6 cut(s)	188, 3774, 3882, 4456, 5753, 7007
BspHI	TCATGA	3 cut(s)	3643, 4375, 4599
BspMAI	CTGCAG	6 cut(s)	582, 2208, 2541, 5644, 6898, 7372
BspMI	ACCTGC	1 cut(s)	3122
BspOI	GCTAGC	1 cut(s)	4317
BspQI	GCTCTTC	1 cut(s)	2896
BspT107I	GGYRCC	6 cut(s)	215, 356, 475, 3988, 4450, 7702
BspTI	CTTAAG	4 cut(s)	2173, 2506, 5555, 6809
BspTNI	GGTCTC	1 cut(s)	102
BsrBI	CCGCTC	2 cut(s)	5312, 6566
BsrDI	GCAATG	3 cut(s)	714, 977, 4071
BsrFI	RCCGGY	2 cut(s)	64, 7563
BsrGI	TGTACA	1 cut(s)	7155
BssAI	RCCGGY	2 cut(s)	64, 7563
BssNI	GRCGYC	2 cut(s)	216, 4451
BssSI	CACGAG	3 cut(s)	1051, 4697, 5951
Bst2BI	CACGAG	3 cut(s)	1051, 4697, 5951
Bst2UI	CCWGG	6 cut(s)	693, 893, 1301, 2898, 4799, 6053
BstACI	GRCGYC	2 cut(s)	216, 4451
BstAFI	CTTAAG	4 cut(s)	2173, 2506, 5555, 6809
BstAPI	GCANNNNNTGC	3 cut(s)	668, 902, 3491
BstAUI	TGTACA	1 cut(s)	7155
BstBAI	YACGTR	2 cut(s)	1004, 7232
BstDSI	CCRYGG	8 cut(s)	87, 1807, 4454, 4737, 5247, 5991, 6501, 7005
BstFNI	CGCG	6 cut(s)	188, 3774, 3882, 4456, 5753, 7007
BstH2I	RGCGCY	2 cut(s)	219, 4454
BstHHI	GCGC	7 cut(s)	218, 608, 930, 1684, 3774, 3882, 4453
BstMCI	CGRYCG	1 cut(s)	69
BstNI	CCWGG	6 cut(s)	693, 893, 1301, 2898, 4799, 6053
BstNSI	RCATGY	8 cut(s)	1276, 3561, 4063, 5678, 5864, 6932, 7106, 7341
BstPAI	GACNNNNGTC	2 cut(s)	2945, 4524
BstSLI	GKGCMC	3 cut(s)	480, 1003, 7301
BstUI	CGCG	6 cut(s)	188, 3774, 3882, 4456, 5753, 7007
BstV2I	GAAGAC	8 cut(s)	2634, 2945, 3471, 3486, 4074, 4610, 5399, 6653
BstXI	CCANNNNNNTGG	4 cut(s)	5197, 5254, 6508, 7784
Bsu15I	ATCGAT	1 cut(s)	587
BsuI	GTATCC	2 cut(s)	1376, 7384
BsuTUI	ATCGAT	1 cut(s)	587
BtgI	CCRYGG	8 cut(s)	87, 1807, 4454, 4737, 5247, 5991, 6501, 7005
BtgZI	GCGATG	1 cut(s)	7734
BtrI	CACGTC	2 cut(s)	1375, 4446
BtsI	GCAGTG	3 cut(s)	903, 7116, 7626
BveI	ACCTGC	1 cut(s)	3122
CaiI	CAGNNNCTG	1 cut(s)	1985
CciI	TCATGA	3 cut(s)	3643, 4375, 4599
CfoI	GCGC	7 cut(s)	218, 608, 930, 1684, 3774, 3882, 4453
Cfr10I	RCCGGY	2 cut(s)	64, 7563
Cfr42I	CCGCGG	2 cut(s)	4457, 7008
ClaI	ATCGAT	1 cut(s)	587
CseI	GACGC	3 cut(s)	862, 1661, 3491
CspAI	ACCGGT	1 cut(s)	64
CspCI	CAANNNNNGTGG	6 cut(s)	582, 617, 2731, 2766, 3172, 3207
DinI	GGCGCC	2 cut(s)	217, 4452
DraI	TTTAAA	4 cut(s)	1221, 4026, 4662, 5916
DraIII	CACNNNGTG	1 cut(s)	472
DrdI	GACNNNNNNGTC	2 cut(s)	116, 5162
DseDI	GACNNNNNNGTC	2 cut(s)	116, 5162
EaeI	YGGCCR	2 cut(s)	3, 3659
EciI	GGCGGA	1 cut(s)	2075
Ecl136II	GAGCTC	2 cut(s)	4498, 7249
Eco24I	GRGCYC	5 cut(s)	4500, 5126, 6380, 7251, 7301
Eco31I	GGTCTC	1 cut(s)	102
Eco32I	GATATC	2 cut(s)	4722, 5976
Eco47I	GGWCC	8 cut(s)	83, 519, 1018, 1187, 4519, 5768, 7022, 7221
Eco53kI	GAGCTC	2 cut(s)	4498, 7249
Eco72I	CACGTG	1 cut(s)	7232
Eco88I	CYCGRG	1 cut(s)	7097
EcoICRI	GAGCTC	2 cut(s)	4498, 7249
EcoO109I	RGGNCCY	3 cut(s)	83, 1187, 7221
EcoRI	GAATTC	2 cut(s)	2084, 2417
EcoRII	CCWGG	6 cut(s)	691, 891, 1299, 2896, 4797, 6051
EcoRV	GATATC	2 cut(s)	4722, 5976
EcoT22I	ATGCAT	5 cut(s)	907, 1137, 4061, 5676, 6930
EcoT38I	GRGCYC	5 cut(s)	4500, 5126, 6380, 7251, 7301
EgeI	GGCGCC	2 cut(s)	217, 4452
EheI	GGCGCC	2 cut(s)	217, 4452
FalI	AAGNNNNNCTT	4 cut(s)	1185, 1217, 3305, 3337
FauI	CCCGC	7 cut(s)	233, 5060, 5392, 5425, 6646, 6679, 7288
FauNDI	CATATG	3 cut(s)	1921, 5099, 6353
FbaI	TGATCA	1 cut(s)	1887
FblI	GTMKAC	4 cut(s)	69, 7001, 7600, 7662
FriOI	GRGCYC	5 cut(s)	4500, 5126, 6380, 7251, 7301
FspAI	RTGCGCAY	1 cut(s)	1683
FspI	TGCGCA	2 cut(s)	929, 1683
GlaI	GCGC	7 cut(s)	217, 607, 929, 1683, 3773, 3881, 4452
GsaI	CCCAGC	1 cut(s)	1789
GsuI	CTGGAG	4 cut(s)	2684, 5057, 6311, 7806
HaeII	RGCGCY	2 cut(s)	219, 4454
HgaI	GACGC	3 cut(s)	862, 1661, 3491
HhaI	GCGC	7 cut(s)	218, 608, 930, 1684, 3774, 3882, 4453
Hin1I	GRCGYC	2 cut(s)	216, 4451
Hin6I	GCGC	7 cut(s)	216, 606, 928, 1682, 3772, 3880, 4451
HinP1I	GCGC	7 cut(s)	216, 606, 928, 1682, 3772, 3880, 4451
HincII	GTYRAC	5 cut(s)	70, 3027, 3041, 3511, 4161
HindII	GTYRAC	5 cut(s)	70, 3027, 3041, 3511, 4161
HindIII	AAGCTT	2 cut(s)	3515, 4484
HpaI	GTTAAC	1 cut(s)	4161
HphI	GGTGA	7 cut(s)	1679, 2027, 2360, 3146, 4654, 5908, 7214
Hpy99I	CGWCG	6 cut(s)	182, 188, 1655, 4444, 4447, 5066
Hsp92I	GRCGYC	2 cut(s)	216, 4451
HspAI	GCGC	7 cut(s)	216, 606, 928, 1682, 3772, 3880, 4451
KasI	GGCGCC	2 cut(s)	215, 4450
KflI	GGGWCCC	2 cut(s)	83, 7221
KpnI	GGTACC	1 cut(s)	7706
Ksp22I	TGATCA	1 cut(s)	1887
KspAI	GTTAAC	1 cut(s)	4161
KspI	CCGCGG	2 cut(s)	4457, 7008
LguI	GCTCTTC	1 cut(s)	2896
MbiI	CCGCTC	2 cut(s)	5312, 6566
MhlI	GDGCHC	9 cut(s)	480, 1003, 4500, 5126, 5514, 6380, 6768, 7251, 7301
MlsI	TGGCCA	1 cut(s)	3661
MluNI	TGGCCA	1 cut(s)	3661
Mly113I	GGCGCC	2 cut(s)	216, 4451
Mox20I	TGGCCA	1 cut(s)	3661
Mph1103I	ATGCAT	5 cut(s)	907, 1137, 4061, 5676, 6930
MroXI	GAANNNNTTC	4 cut(s)	1674, 2757, 2838, 4397
MscI	TGGCCA	1 cut(s)	3661
MslI	CAYNNNNRTG	8 cut(s)	850, 994, 2031, 2364, 4268, 5252, 6506, 6696
Msp20I	TGGCCA	1 cut(s)	3661
MspCI	CTTAAG	4 cut(s)	2173, 2506, 5555, 6809
Mva1269I	GAATGC	3 cut(s)	356, 1102, 3573
MvaI	CCWGG	6 cut(s)	693, 893, 1301, 2898, 4799, 6053
MvnI	CGCG	6 cut(s)	188, 3774, 3882, 4456, 5753, 7007
NarI	GGCGCC	2 cut(s)	216, 4451
NciI	CCSGG	7 cut(s)	30, 81, 311, 5121, 5571, 6375, 6825
NcoI	CCATGG	5 cut(s)	1807, 4737, 5247, 5991, 6501
NdeI	CATATG	3 cut(s)	1921, 5099, 6353
NheI	GCTAGC	1 cut(s)	4313
NmeAIII	GCCGAG	2 cut(s)	1425, 1483
NmuCI	GTSAC	6 cut(s)	442, 4642, 5360, 5896, 7340, 7480
NsbI	TGCGCA	2 cut(s)	929, 1683
NsiI	ATGCAT	5 cut(s)	907, 1137, 4061, 5676, 6930
NspI	RCATGY	8 cut(s)	1276, 3561, 4063, 5678, 5864, 6932, 7106, 7341
OliI	CACNNNNGTG	1 cut(s)	850
PaeI	GCATGC	4 cut(s)	5678, 5864, 6932, 7106
PaeR7I	CTCGAG	1 cut(s)	7097
PagI	TCATGA	3 cut(s)	3643, 4375, 4599
PaqCI	CACCTGC	1 cut(s)	3122
PciI	ACATGT	1 cut(s)	3557
PciSI	GCTCTTC	1 cut(s)	2896
PctI	GAATGC	3 cut(s)	356, 1102, 3573
PdmI	GAANNNNTTC	4 cut(s)	1674, 2757, 2838, 4397
Pfl23II	CGTACG	1 cut(s)	2253
PflFI	GACNNNGTC	1 cut(s)	6130
PflMI	CCANNNNNTGG	2 cut(s)	472, 1325
PfoI	TCCNGGA	2 cut(s)	4797, 6051
PinAI	ACCGGT	1 cut(s)	64
PluTI	GGCGCC	2 cut(s)	219, 4454
PmaCI	CACGTG	1 cut(s)	7232
PmlI	CACGTG	1 cut(s)	7232
Ppu21I	YACGTR	2 cut(s)	1004, 7232
PpuMI	RGGWCCY	3 cut(s)	83, 1187, 7221
PscI	ACATGT	1 cut(s)	3557
PshAI	GACNNNNGTC	2 cut(s)	2945, 4524
PshBI	ATTAAT	1 cut(s)	1961
Psp124BI	GAGCTC	2 cut(s)	4500, 7251
Psp5II	RGGWCCY	3 cut(s)	83, 1187, 7221
Psp6I	CCWGG	6 cut(s)	691, 891, 1299, 2896, 4797, 6051
PspCI	CACGTG	1 cut(s)	7232
PspFI	CCCAGC	1 cut(s)	1785
PspGI	CCWGG	6 cut(s)	691, 891, 1299, 2896, 4797, 6051
PspLI	CGTACG	1 cut(s)	2253
PspOMI	GGGCCC	1 cut(s)	7297
PspPPI	RGGWCCY	3 cut(s)	83, 1187, 7221
PspXI	VCTCGAGB	1 cut(s)	7097
PsrI	GAACNNNNNNTAC	4 cut(s)	4794, 4826, 6048, 6080
PstI	CTGCAG	6 cut(s)	582, 2208, 2541, 5644, 6898, 7372
PstNI	CAGNNNCTG	1 cut(s)	1985
PsyI	GACNNNGTC	1 cut(s)	6130
PvuII	CAGCTG	8 cut(s)	1082, 2153, 4214, 4841, 5416, 6095, 6670, 7766
RseI	CAYNNNNRTG	8 cut(s)	850, 994, 2031, 2364, 4268, 5252, 6506, 6696
SacI	GAGCTC	2 cut(s)	4500, 7251
SacII	CCGCGG	2 cut(s)	4457, 7008
SalI	GTCGAC	1 cut(s)	68
SapI	GCTCTTC	1 cut(s)	2896
ScaI	AGTACT	2 cut(s)	4912, 6166
SduI	GDGCHC	9 cut(s)	480, 1003, 4500, 5126, 5514, 6380, 6768, 7251, 7301
SfiI	GGCCNNNNNGGCC	1 cut(s)	164
SfoI	GGCGCC	2 cut(s)	217, 4452
Sfr274I	CTCGAG	1 cut(s)	7097
Sfr303I	CCGCGG	2 cut(s)	4457, 7008
SgrBI	CCGCGG	2 cut(s)	4457, 7008
SinI	GGWCC	8 cut(s)	83, 519, 1018, 1187, 4519, 5768, 7022, 7221
SlaI	CTCGAG	1 cut(s)	7097
SmiI	ATTTAAAT	1 cut(s)	1221
SmiMI	CAYNNNNRTG	8 cut(s)	850, 994, 2031, 2364, 4268, 5252, 6506, 6696
SphI	GCATGC	4 cut(s)	5678, 5864, 6932, 7106
SspDI	GGCGCC	2 cut(s)	215, 4450
SspI	AATATT	3 cut(s)	3021, 4428, 7176
SstI	GAGCTC	2 cut(s)	4500, 7251
SwaI	ATTTAAAT	1 cut(s)	1221
TatI	WGTACW	6 cut(s)	3470, 4360, 4489, 4910, 6164, 7155
TauI	GCSGC	5 cut(s)	8, 491, 4456, 4459, 7109
TseFI	GTSAC	6 cut(s)	442, 4642, 5360, 5896, 7340, 7480
Tsp45I	GTSAC	6 cut(s)	442, 4642, 5360, 5896, 7340, 7480
TspGWI	ACGGA	6 cut(s)	10, 1378, 1826, 1957, 1985, 7812
Tth111I	GACNNNGTC	1 cut(s)	6130
Van91I	CCANNNNNTGG	2 cut(s)	472, 1325
Vha464I	CTTAAG	4 cut(s)	2173, 2506, 5555, 6809
VpaK11BI	GGWCC	8 cut(s)	83, 519, 1018, 1187, 4519, 5768, 7022, 7221
VspI	ATTAAT	1 cut(s)	1961
XbaI	TCTAGA	3 cut(s)	5482, 6736, 7243
XceI	RCATGY	8 cut(s)	1276, 3561, 4063, 5678, 5864, 6932, 7106, 7341
XcmI	CCANNNNNNNNNTGG	3 cut(s)	4513, 5863, 7177
XhoI	CTCGAG	1 cut(s)	7097
XmiI	GTMKAC	4 cut(s)	69, 7001, 7600, 7662
XmnI	GAANNNNTTC	4 cut(s)	1674, 2757, 2838, 4397
ZrmI	AGTACT	2 cut(s)	4912, 6166
Zsp2I	ATGCAT	5 cut(s)	907, 1137, 4061, 5676, 6930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.