Rroxscaffold_3G00255340

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002032
Physical Location & Seq
Reverse (-)
49556818 .. 49570392
13575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255340.1

Sequence Viewer

Length: 1923 bp
ATGAGAGAATTTTCTGATATTTCATACGTTCTTCCGGCAGACGATGTATGTATCGCATCTGACGAAGACTACGAGGAATTTGATTCAAGAACTTCAATACTGGAGAAAATCTTGAAGGAATTGGGAAATTCTAGTTCTGAAATGATTTTGGTGTATGGTGTTGGGGGTGTGGGAAAGACAACGCTCGTTGAAGAAGTTCTAAGGAAAACTCAAAAAGAGAAATTTTTTGAGGATGTAGTAATGGTACGGGATGTGAAAATTCCAAACTTGGAAGCAATTCAAAATCAAATTGCCGACAAGTTAGGTCTGGACATTTCAGAAATTAAGACTCTCCCTGGAAGAGCCGATAAAATAAATGAAAGAATCAAGGACAAGAAGACTCTTGTCATTTTTGATGATGTTTGGGGAGAACTTGATTTGAAGGCTGTGGGACTTCCCCACAAATCCACTTGTAGAATATTGTTGACATCCAGAAGTCAACAAGTATTTTCTCATAAGATTAAGCAAAAAGAGTTTCTGCTTGGGATTTTAGATGAAAAAGAAGCATGGGCTTTATTTGAGAAGAAAGCAGGTGATGTTGTTTCAGATCCTACGATTCAAAATGTAGCAACTGAAGTAGCCAAAAAATGTGGAGGGTTGCCTGTTTTTATTATCACTGTTGCAAGAGCTTTAGAAAACAAAACCCTACCCATCTGGAAAAATGCCTTAAGATGCCTCGAAAGGTTTGATGAGAAAGGAGACTTAACTGAAAAGGCATACTTGGGTATTGAGTGGAGTTACAAGCAATTGAGTGTCAAGGATCAGCTTCAGCCGTTGTTCTTGTTGTGTGGTCTTATTGCAGAGGGCAACTATGTTTCATTTACAGACTTGTTGAAATATAGTATAGGTTTGGGTTTGTTTGAAGACTGTACTGTGGAAGACGCACGAAATGCCTTGTATCAACAGGTTGACAAGCTTAAAAGTTCTTGTCTATTGCTAGACGACGATGATGGTACATGCGTCAGAATGCACGATCTTGTACATGATGTTGCTAACAGGATTGCATACAGAGATCATCATGTGTTAGTTGAAGATGGAGGTCATGAGAGGAAAAAATGGCCAAATGAAGATTTCTTTACTAAGTGCAGTAAGATCTGTTTGCCTTGTCGCAATATCGCCGCCCTTCCTCAAGTACCTTGGCAATGCCCGAAATTAGAGATGTTTCATTTGCACCGTGGAAGTGATGATTTCTCGCCTGAAATACCAAGCAACTTTTTCGTGGAGATGAAAAAACTCAAAGTCTTGGGTTTAAGCAATGTGCCACTTCTTCCACTACCCCCATCTTTTTCATTTTTAAAGAATCTCCAAACATTGTGTTTAGATGGCTGCATGTTTGAAGACATTGCTGTAGTTGGAGAGCTAAGAAACTTAGAAGTTCTTAGCCTTGAAAAATCCGAGGTTAAACGATTACCCAAAGAAATTGGGCAGTTAACCCGTCTGCGGTTGGTGGATTTGTCTGATTGCTATGGACTTAAAGTGATTCCAGCTGGTGTCATTTCAAATTGGACAAGACTTGAAGACTTGAGAATGAGAGACAGCTTTCAGAATTGGGTGAGTGGAGAAGTCATCTGTGAAACAAGTAATGCTAGCCTTTTGGAGCTGATGAACTTGCCCAAGCTAACCGCATTAGAAGTACGCATTCCAGATCCCGACATCCTTCCAGCAAAGTTTTTGTCTGACAGGTGGGAAAAATACCAAATATTTATTGAGCCGAAGGGGTGGTCGTGTGGTATGTATGAAACAACCCTCAACACGCTTGTACTAGGGCTCACTACCAGATATGAATTGGACCAAGGTCTAAAAGTATTACTGGAGAGATGTGAAGATTTATACTTTATTGGGACGGAAGATGTTGATTTTTCATGTATCATGAAGACTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

640

Amino Acids

72.77

Weight (kDa)

5.12

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 33 - 191 3e-30 NB-ARC domain
AAA_16 PF13191 33 - 104 4e-06 AAA ATPase domain
LRR_14 PF23598 464 - 560 1e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 560
Acc36I ACCTGC 1 cut(s) 560
AciI CCGC 3 cut(s) 1158, 1480, 1662
AclWI GGATC 3 cut(s) 581, 807, 1679
AcoI YGGCCR 1 cut(s) 1097
AcsI RAATTY 5 cut(s) 8, 77, 127, 221, 258
AcuI CTGAAG 2 cut(s) 633, 791
AfaI GTAC 7 cut(s) 246, 910, 994, 1020, 1173, 1674, 1800
AfiI CCNNNNNNNGG 1 cut(s) 1479
AflII CTTAAG 1 cut(s) 706
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 8 cut(s) 668, 805, 955, 1399, 1526, 1578, 1639, 1657
AluI AGCT 8 cut(s) 668, 805, 955, 1399, 1526, 1578, 1639, 1657
Alw26I GTCTC 2 cut(s) 732, 1566
AlwI GGATC 3 cut(s) 581, 807, 1679
AoxI GGCC 1 cut(s) 1097
ApeKI GCWGC 1 cut(s) 1365
ApoI RAATTY 5 cut(s) 8, 77, 127, 221, 258
ArsI GACNNNNNNTTYG 4 cut(s) 1697, 1729, 1745, 1777
Asp700I GAANNNNTTC 2 cut(s) 195, 276
AspS9I GGNCC 1 cut(s) 1828
AsuHPI GGTGA 2 cut(s) 584, 1603
AsuNHI GCTAGC 1 cut(s) 1625
AvaII GGWCC 1 cut(s) 1828
BaeI ACNNNNGTAYC 2 cut(s) 984, 1017
BalI TGGCCA 1 cut(s) 1099
BanII GRGCYC 1 cut(s) 1809
BbsI GAAGAC 7 cut(s) 72, 383, 909, 924, 1383, 1563, 1919
BbvI GCAGC 1 cut(s) 1352
BccI CCATC 5 cut(s) 698, 983, 1067, 1327, 1355
BceAI ACGGC 1 cut(s) 796
BcgI CGANNNNNNTGC 2 cut(s) 1237, 1271
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 2 cut(s) 732, 1566
BfaI CTAG 4 cut(s) 132, 977, 1626, 1802
BfmI CTRYAG 1 cut(s) 1386
BfrI CTTAAG 1 cut(s) 706
BfuAI ACCTGC 1 cut(s) 560
BglII AGATCT 1 cut(s) 1131
BisI GCNGC 2 cut(s) 1158, 1366
BlsI GCNGC 2 cut(s) 1159, 1367
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 1828
BmgT120I GGNCC 1 cut(s) 1828
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 2 cut(s) 65, 701
BmtI GCTAGC 1 cut(s) 1629
BoxI GACNNNNGTC 2 cut(s) 383, 1833
BpiI GAAGAC 7 cut(s) 72, 383, 909, 924, 1383, 1563, 1919
BpmI CTGGAG 2 cut(s) 122, 1871
BpuEI CTTGAG 2 cut(s) 1152, 1582
BsaJI CCNNGG 5 cut(s) 334, 1175, 1213, 1434, 1831
Bsc4I CCNNNNNNNGG 1 cut(s) 1479
Bse1I ACTGG 2 cut(s) 105, 1854
Bse3DI GCAATG 3 cut(s) 1187, 1300, 1380
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 5 cut(s) 334, 1175, 1213, 1434, 1831
BseGI GGATG 4 cut(s) 238, 256, 467, 1692
BseLI CCNNNNNNNGG 1 cut(s) 1479
BseMI GCAATG 3 cut(s) 1187, 1300, 1380
BseNI ACTGG 2 cut(s) 105, 1854
BseXI GCAGC 1 cut(s) 1352
BsgI GTGCAG 1 cut(s) 1144
BshFI GGCC 1 cut(s) 1099
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 2 cut(s) 444, 1894
BslI CCNNNNNNNGG 1 cut(s) 1479
BsmAI GTCTC 2 cut(s) 732, 1566
BsmFI GGGAC 2 cut(s) 444, 1894
BsmI GAATGC 2 cut(s) 1011, 1677
BsnI GGCC 1 cut(s) 1099
Bsp1286I GDGCHC 1 cut(s) 1809
Bsp1407I TGTACA 1 cut(s) 1018
Bsp143I GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
BspACI CCGC 3 cut(s) 1158, 1480, 1662
BspANI GGCC 1 cut(s) 1099
BspHI TCATGA 2 cut(s) 1081, 1908
BspMI ACCTGC 1 cut(s) 560
BspOI GCTAGC 1 cut(s) 1629
BspPI GGATC 3 cut(s) 581, 807, 1679
BspQI GCTCTTC 1 cut(s) 334
BspTI CTTAAG 1 cut(s) 706
BsrDI GCAATG 3 cut(s) 1187, 1300, 1380
BsrGI TGTACA 1 cut(s) 1018
BsrI ACTGG 2 cut(s) 105, 1854
BssECI CCNNGG 5 cut(s) 334, 1175, 1213, 1434, 1831
BssMI GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
BssT1I CCWWGG 2 cut(s) 1175, 1831
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 4 cut(s) 658, 908, 913, 1214
Bst6I CTCTTC 1 cut(s) 334
BstAFI CTTAAG 1 cut(s) 706
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 1 cut(s) 1018
BstC8I GCNNGC 1 cut(s) 1627
BstDEI CTNAG 5 cut(s) 200, 1119, 1400, 1408, 1418
BstDSI CCRYGG 1 cut(s) 1213
BstF5I GGATG 4 cut(s) 238, 256, 467, 1692
BstKTI GATC 6 cut(s) 589, 802, 1015, 1054, 1134, 1687
BstMAI GTCTC 2 cut(s) 732, 1566
BstMBI GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
BstMWI GCNNNNNNNGC 1 cut(s) 929
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 2 cut(s) 999, 1372
BstPAI GACNNNNGTC 2 cut(s) 383, 1833
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 1 cut(s) 1386
BstV1I GCAGC 1 cut(s) 1352
BstV2I GAAGAC 7 cut(s) 72, 383, 909, 924, 1383, 1563, 1919
BstX2I RGATCY 3 cut(s) 586, 1131, 1684
BstYI RGATCY 3 cut(s) 586, 1131, 1684
BsuRI GGCC 1 cut(s) 1099
BtgI CCRYGG 1 cut(s) 1213
BtsCI GGATG 4 cut(s) 238, 256, 467, 1692
BtsIMutI CAGTG 1 cut(s) 654
BveI ACCTGC 1 cut(s) 560
Cac8I GCNNGC 1 cut(s) 1627
CciI TCATGA 2 cut(s) 1081, 1908
Cfr13I GGNCC 1 cut(s) 1828
CseI GACGC 2 cut(s) 929, 988
Csp6I GTAC 7 cut(s) 245, 909, 993, 1019, 1172, 1673, 1799
CspCI CAANNNNNGTGG 2 cut(s) 610, 645
CviAII CATG 8 cut(s) 546, 996, 1022, 1058, 1082, 1369, 1902, 1909
CviQI GTAC 7 cut(s) 245, 909, 993, 1019, 1172, 1673, 1799
DdeI CTNAG 5 cut(s) 200, 1119, 1400, 1408, 1418
DpnI GATC 6 cut(s) 588, 801, 1014, 1053, 1133, 1686
DpnII GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
DraI TTTAAA 1 cut(s) 1335
EaeI YGGCCR 1 cut(s) 1097
Eam1104I CTCTTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 1175, 1831
Eco24I GRGCYC 1 cut(s) 1809
Eco47I GGWCC 1 cut(s) 1828
Eco57I CTGAAG 2 cut(s) 633, 791
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 1175, 1831
EcoT38I GRGCYC 1 cut(s) 1809
ErhI CCWWGG 2 cut(s) 1175, 1831
FaeI CATG 8 cut(s) 549, 999, 1025, 1061, 1085, 1372, 1905, 1912
FalI AAGNNNNNCTT 2 cut(s) 743, 775
FaqI GGGAC 2 cut(s) 444, 1894
FatI CATG 8 cut(s) 545, 995, 1021, 1057, 1081, 1368, 1901, 1908
Fnu4HI GCNGC 2 cut(s) 1158, 1366
FokI GGATG 4 cut(s) 245, 263, 454, 1679
FriOI GRGCYC 1 cut(s) 1809
Fsp4HI GCNGC 2 cut(s) 1158, 1366
FspBI CTAG 4 cut(s) 132, 977, 1626, 1802
GluI GCNGC 2 cut(s) 1158, 1366
GsuI CTGGAG 2 cut(s) 122, 1871
HaeIII GGCC 1 cut(s) 1099
HapII CCGG 1 cut(s) 35
HgaI GACGC 2 cut(s) 929, 988
Hin1II CATG 8 cut(s) 549, 999, 1025, 1061, 1085, 1372, 1905, 1912
HincII GTYRAC 4 cut(s) 465, 479, 949, 1470
HindII GTYRAC 4 cut(s) 465, 479, 949, 1470
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 7 cut(s) 83, 328, 363, 379, 595, 1339, 1519
HpaI GTTAAC 1 cut(s) 1470
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 584, 1603
Hpy166II GTNNAC 4 cut(s) 465, 479, 949, 1470
Hpy188III TCNNGA 9 cut(s) 87, 112, 308, 471, 694, 1082, 1682, 1688, 1909
Hpy8I GTNNAC 4 cut(s) 465, 479, 949, 1470
Hpy99I CGWCG 1 cut(s) 986
HpyAV CCTTC 5 cut(s) 109, 415, 1172, 1706, 1747
HpyCH4III ACNGT 4 cut(s) 658, 908, 913, 1214
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 7 cut(s) 662, 839, 1009, 1043, 1125, 1210, 1368
HpyF10VI GCNNNNNNNGC 1 cut(s) 929
HpyF3I CTNAG 5 cut(s) 200, 1119, 1400, 1408, 1418
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 8 cut(s) 549, 999, 1025, 1061, 1085, 1372, 1905, 1912
KspAI GTTAAC 1 cut(s) 1470
Kzo9I GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
LguI GCTCTTC 1 cut(s) 334
LmnI GCTCC 1 cut(s) 1636
Lsp1109I GCAGC 1 cut(s) 1352
LweI GCATC 2 cut(s) 65, 701
MaeI CTAG 4 cut(s) 132, 977, 1626, 1802
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 776
MalI GATC 6 cut(s) 588, 801, 1014, 1053, 1133, 1686
MboI GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
MfeI CAATTG 1 cut(s) 785
MflI RGATCY 3 cut(s) 586, 1131, 1684
MhlI GDGCHC 1 cut(s) 1809
MlsI TGGCCA 1 cut(s) 1099
MluNI TGGCCA 1 cut(s) 1099
MlyI GAGTC 2 cut(s) 322, 373
MmeI TCCRAC 1 cut(s) 1372
Mox20I TGGCCA 1 cut(s) 1099
MroXI GAANNNNTTC 2 cut(s) 195, 276
MscI TGGCCA 1 cut(s) 1099
Msp20I TGGCCA 1 cut(s) 1099
MspA1I CMGCKG 1 cut(s) 1526
MspCI CTTAAG 1 cut(s) 706
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 336
MunI CAATTG 1 cut(s) 785
Mva1269I GAATGC 2 cut(s) 1011, 1677
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 929
NdeII GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
NheI GCTAGC 1 cut(s) 1625
NlaIII CATG 8 cut(s) 549, 999, 1025, 1061, 1085, 1372, 1905, 1912
NspI RCATGY 2 cut(s) 999, 1372
PagI TCATGA 2 cut(s) 1081, 1908
PaqCI CACCTGC 1 cut(s) 560
PciSI GCTCTTC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 69
PctI GAATGC 2 cut(s) 1011, 1677
PdmI GAANNNNTTC 2 cut(s) 195, 276
PfeI GAWTC 5 cut(s) 83, 363, 595, 1339, 1519
PkrI GCNGC 2 cut(s) 1159, 1367
PleI GAGTC 2 cut(s) 322, 373
PpsI GAGTC 2 cut(s) 322, 373
PshAI GACNNNNGTC 2 cut(s) 383, 1833
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspPI GGNCC 1 cut(s) 1828
PsuI RGATCY 3 cut(s) 586, 1131, 1684
PvuII CAGCTG 1 cut(s) 1526
RsaI GTAC 7 cut(s) 246, 910, 994, 1020, 1173, 1674, 1800
RsaNI GTAC 7 cut(s) 245, 909, 993, 1019, 1172, 1673, 1799
SapI GCTCTTC 1 cut(s) 334
SatI GCNGC 2 cut(s) 1158, 1366
Sau3AI GATC 6 cut(s) 586, 799, 1012, 1051, 1131, 1684
Sau96I GGNCC 1 cut(s) 1828
SchI GAGTC 2 cut(s) 322, 373
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 1809
SfaNI GCATC 2 cut(s) 65, 701
SfcI CTRYAG 1 cut(s) 1386
SinI GGWCC 1 cut(s) 1828
SmlI CTYRAG 3 cut(s) 706, 1167, 1561
SmoI CTYRAG 3 cut(s) 706, 1167, 1561
SsiI CCGC 3 cut(s) 1158, 1480, 1662
SspI AATATT 2 cut(s) 459, 1740
SspMI CTAG 4 cut(s) 132, 977, 1626, 1802
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 2 cut(s) 1175, 1831
TaaI ACNGT 4 cut(s) 658, 908, 913, 1214
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 717
TatI WGTACW 3 cut(s) 908, 1018, 1798
TauI GCSGC 1 cut(s) 1160
TfiI GAWTC 5 cut(s) 83, 363, 595, 1339, 1519
TscAI CASTG 1 cut(s) 661
TseI GCWGC 1 cut(s) 1365
TspGWI ACGGA 1 cut(s) 1898
TspRI CASTG 1 cut(s) 661
Vha464I CTTAAG 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 1828
XapI RAATTY 5 cut(s) 8, 77, 127, 221, 258
XceI RCATGY 2 cut(s) 999, 1372
XcmI CCANNNNNNNNNTGG 1 cut(s) 1822
XmnI GAANNNNTTC 2 cut(s) 195, 276
XspI CTAG 4 cut(s) 132, 977, 1626, 1802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.