Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0007521

Physical Location & Seq

Genomic Coordinates Forward (+)

4705530 .. 4715375

Gene Structure 9846 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010839

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGAACCTCGTCGCCCAGGCCCAGTTCGCCAATGTCAAGGTTGCGGTGAAAATAGTGATCACGGCGGTCCACTCAATAGGTGGCCCAAGGAAGATGGTGGCGGTCGCCAGGCACTTGCCGCAAGCCTCGCGGCCAAGATTAAGCGAGATGCTTGGACACGGGTCCAAGGCGTCTCCTCTGGCCGCGCTCTCCTTGGCCGCCGCTCTGGTGGCACATCTGACTTCTCTCTCCATGGCCGCCGCCGTGGCGAGGTGTCCTCTTATGGGCGTGCGGCAAGTGCCTGGCAGCCGCCACCATCTTCCTTGGGCCACCTATTGAGTGGACCGCCGAGGAGATTCTTTCAACCCCTTGGGATAAAGAAAACGATAGGTGAAGCGATTGCTACTAATCGGGGCGGCGCGGCCGTGGCTCAGAGAAGCCGCCGCCGTGGCAAGGCTGTCCTGGCGTCTGGCGACACCACCTCTGGTGGTAACTCCTCGGATCGTCAAAGTGCTCGTCAGAGTGATGAAGAAAACACTACGGTGGTGGTTGTTCAAGCTGTCCCTGAGCCACTGCAGGGGATTCCAGAGGTTGTTCCGGCTGTTCATCAACCAATTGCACCAGATCATGCATCAATTGATATGGTCGCAATTGAAGACATTGAAGATGAAGAAGGGATCCACAGCTCAGGGAGTCGAAAAACTTTTACCATGAAACCATTGCCTCTAAAAGCATCACGGGACATCTTGAGGTTGATGCACCATACTTACCGCAGTCAGGGATTTCAGTTGTTTGTAAGCTTCTTGGAAATATATGGAGGAAAACTTTTTGATCTCCTCAATGAGCGCAAAAAGCTTTGCATGAGAGAAGATGGTAAGCAACAAGTTTGTATTGTGGGTTTGCAAGAATATAGTGTGTCTGATGCAGAGACAATTAAAGAGCTTATTGAGAAAGGAAGTGCCACAATAAGTACTGGTACCACTGGTGCAAATGAAGAATCCTCTCGCTCACATGCCGTACTTCAGCTTGCTATCAAGAGGGTAGTTGATGGCAATGTATCAAAGCCTCCCCGTCTTGTTGGTAAACTCTCCTTTATAGATCTTGCTGGAAGTGAATGTGGTGCAGATACTACAGATAATGACAAACAGACAAGAAAAATGCGGGGATGTTTATGCTTTGCTTTTGCATCTACTGGCGGTTGGAAGTGGCCTTTTGATTGGTGGATTAGAGGCTTGAGAAGAGGAAGATATATGCATGTTTGGCCAGCCTTCGTTGGATATAGTGTGCTAGGGTATCCTCATATTATAATACAGGGCTGGACTTTTAGTATGTGTTTGTCGCTTGGAGCGTTTTATGCTTGTCTTATTAGAGAGGAGGATGAGAGGCTATCCGCTATCCTTATTGGATATGGACGACATGAAGACCACAGTTTTCTTCTTGAGTCACTGCATCAAATCGGAGAACCGTTCAATAACCACTCCATCAGCATTGGCAGGGTATTTGATCCGGAGGATGATGCCGCAAAAGACAGTGGCTATGGCATTGCACTCAGTGCAAAGAAGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005856 GO:0005871 GO:0005875 GO:0005886 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:0071944 GO:1903338

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

516

Amino Acids

56.32

Weight (kDa)

8.95

Isoelectric Point (pI)

55.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	223 - 378	3.8e-34	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007521)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g31460 FvH4_6g31460 FvH4_6g31460
malus_domestica	MD15G1185700.v1.1
pyrus_communis	pycom09g12870
rosa_chinensis	RchiOBHm_Chr2g0085031 RchiOBHm_Chr2g0113281
rosa_laevigata	RLG00000010839 RLG00000019129 RLG00000034360 RLG00000034995
rosa_roxburghii	Rroxscaffold_1G00003470 Rroxscaffold_1G00033120 Rroxscaffold_5G00342210 Rroxscaffold_5G00342360
rosa_rugosa	Rorug04G0188100.1
rosa_samantha	Rh1AG098400 Rh1AG203700 Rh2AG396900 Rh2AG398300 Rh2AG398400 Rh2BG258800 Rh2BG310100 Rh2CG383400 Rh5BG449800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1286
Acc65I	GGTACC	1 cut(s)	956
AccB1I	GGYRCC	1 cut(s)	956
AccBSI	CCGCTC	1 cut(s)	204
AccII	CGCG	3 cut(s)	131, 186, 401
AccIII	TCCGGA	1 cut(s)	1486
AclWI	GGATC	4 cut(s)	489, 652, 665, 1478
AcoI	YGGCCR	6 cut(s)	132, 181, 196, 235, 402, 1241
AcuI	CTGAAG	1 cut(s)	986
AcyI	GRCGYC	2 cut(s)	171, 446
AdeI	CACNNNGTG	1 cut(s)	1532
AfaI	GTAC	3 cut(s)	952, 958, 999
AfiI	CCNNNNNNNGG	5 cut(s)	250, 264, 433, 557, 757
AgsI	TTSAA	5 cut(s)	344, 536, 635, 644, 1450
AjnI	CCWGG	4 cut(s)	16, 108, 281, 441
AleI	CACNNNNGTG	1 cut(s)	521
AluBI	AGCT	6 cut(s)	539, 666, 780, 835, 922, 1006
AluI	AGCT	6 cut(s)	539, 666, 780, 835, 922, 1006
Alw21I	GWGCWC	1 cut(s)	496
Alw26I	GTCTC	2 cut(s)	178, 902
AlwI	GGATC	4 cut(s)	489, 652, 665, 1478
Aor13HI	TCCGGA	1 cut(s)	1486
ApeKI	GCWGC	1 cut(s)	286
ArsI	GACNNNNNNTTYG	2 cut(s)	481, 513
Asp718I	GGTACC	1 cut(s)	956
AspLEI	GCGC	3 cut(s)	188, 401, 828
AspS9I	GGNCC	6 cut(s)	20, 68, 84, 163, 307, 323
AsuHPI	GGTGA	2 cut(s)	59, 383
AvaII	GGWCC	3 cut(s)	68, 163, 323
BalI	TGGCCA	1 cut(s)	1243
BamHI	GGATCC	1 cut(s)	657
BanI	GGYRCC	1 cut(s)	956
BbsI	GAAGAC	2 cut(s)	642, 1407
Bbv12I	GWGCWC	1 cut(s)	496
BbvI	GCAGC	1 cut(s)	298
BccI	CCATC	5 cut(s)	89, 304, 845, 1022, 1469
BceAI	ACGGC	5 cut(s)	79, 228, 389, 411, 980
BciT130I	CCWGG	4 cut(s)	18, 110, 283, 443
BciVI	GTATCC	1 cut(s)	1284
BclI	TGATCA	1 cut(s)	58
BcoDI	GTCTC	2 cut(s)	178, 902
BfaI	CTAG	1 cut(s)	1268
BfmI	CTRYAG	2 cut(s)	554, 1110
BfuI	GTATCC	1 cut(s)	1284
BglI	GCCNNNNNGGC	2 cut(s)	246, 429
BglII	AGATCT	1 cut(s)	1078
BmcAI	AGTACT	1 cut(s)	952
Bme1390I	CCNGG	4 cut(s)	18, 110, 283, 443
Bme18I	GGWCC	3 cut(s)	68, 163, 323
BmgT120I	GGNCC	6 cut(s)	20, 68, 84, 163, 307, 323
BmiI	GGNNCC	4 cut(s)	6, 164, 659, 958
BmrFI	CCNGG	4 cut(s)	18, 110, 283, 443
BmrI	ACTGGG	1 cut(s)	17
BmsI	GCATC	8 cut(s)	139, 620, 722, 726, 892, 1175, 1438, 1486
BmuI	ACTGGG	1 cut(s)	17
BoxI	GACNNNNGTC	1 cut(s)	161
BpiI	GAAGAC	2 cut(s)	642, 1407
BplI	GAGNNNNNCTC	2 cut(s)	242, 274
Bpu10I	CCTNAGC	2 cut(s)	546, 667
BpuEI	CTTGAG	3 cut(s)	748, 1234, 1439
BsaHI	GRCGYC	2 cut(s)	171, 446
BsaWI	WCCGGW	1 cut(s)	1486
Bsc4I	CCNNNNNNNGG	5 cut(s)	250, 264, 433, 557, 757
Bse1I	ACTGG	4 cut(s)	23, 958, 967, 1177
Bse3DI	GCAATG	3 cut(s)	698, 1039, 1521
BseAI	TCCGGA	1 cut(s)	1486
BseBI	CCWGG	4 cut(s)	18, 110, 283, 443
BseGI	GGATG	3 cut(s)	1151, 1363, 1498
BseLI	CCNNNNNNNGG	5 cut(s)	250, 264, 433, 557, 757
BseMI	GCAATG	3 cut(s)	698, 1039, 1521
BseMII	CTCAG	4 cut(s)	425, 537, 681, 1543
BseNI	ACTGG	4 cut(s)	23, 958, 967, 1177
BseRI	GAGGAG	5 cut(s)	166, 346, 466, 806, 1367
BseX3I	CGGCCG	1 cut(s)	402
BseXI	GCAGC	1 cut(s)	298
BsgI	GTGCAG	1 cut(s)	1122
Bsh1236I	CGCG	3 cut(s)	131, 186, 401
Bsh1285I	CGRYCG	2 cut(s)	106, 405
BshNI	GGYRCC	1 cut(s)	956
BsiEI	CGRYCG	2 cut(s)	106, 405
BsiHKAI	GWGCWC	1 cut(s)	496
BsiSI	CCGG	2 cut(s)	578, 1487
BslFI	GGGAC	2 cut(s)	527, 734
BslI	CCNNNNNNNGG	5 cut(s)	250, 264, 433, 557, 757
BsmAI	GTCTC	2 cut(s)	178, 902
BsmBI	CGTCTC	1 cut(s)	178
BsmFI	GGGAC	2 cut(s)	527, 734
Bsp1286I	GDGCHC	1 cut(s)	496
Bsp13I	TCCGGA	1 cut(s)	1486
Bsp143I	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
Bsp19I	CCATGG	1 cut(s)	232
BspCNI	CTCAG	4 cut(s)	424, 538, 680, 1542
BspEI	TCCGGA	1 cut(s)	1486
BspFNI	CGCG	3 cut(s)	131, 186, 401
BspLI	GGNNCC	4 cut(s)	6, 164, 659, 958
BspMAI	CTGCAG	1 cut(s)	558
BspPI	GGATC	4 cut(s)	489, 652, 665, 1478
BspT107I	GGYRCC	1 cut(s)	956
BsrBI	CCGCTC	1 cut(s)	204
BsrDI	GCAATG	3 cut(s)	698, 1039, 1521
BsrI	ACTGG	4 cut(s)	23, 958, 967, 1177
BssMI	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
BssNI	GRCGYC	2 cut(s)	171, 446
BssT1I	CCWWGG	6 cut(s)	87, 166, 193, 232, 303, 349
Bst2UI	CCWGG	4 cut(s)	18, 110, 283, 443
Bst4CI	ACNGT	4 cut(s)	523, 1409, 1446, 1511
Bst6I	CTCTTC	1 cut(s)	1213
BstACI	GRCGYC	2 cut(s)	171, 446
BstAPI	GCANNNNNTGC	1 cut(s)	1532
BstC8I	GCNNGC	4 cut(s)	124, 270, 1008, 1245
BstDEI	CTNAG	4 cut(s)	411, 546, 667, 1529
BstDSI	CCRYGG	4 cut(s)	232, 244, 405, 427
BstF5I	GGATG	3 cut(s)	1151, 1363, 1498
BstFNI	CGCG	3 cut(s)	131, 186, 401
BstHHI	GCGC	3 cut(s)	188, 401, 828
BstKTI	GATC	7 cut(s)	61, 484, 607, 660, 814, 1081, 1486
BstMAI	GTCTC	2 cut(s)	178, 902
BstMBI	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
BstMCI	CGRYCG	2 cut(s)	106, 405
BstNI	CCWGG	4 cut(s)	18, 110, 283, 443
BstNSI	RCATGY	2 cut(s)	995, 1238
BstPAI	GACNNNNGTC	1 cut(s)	161
BstSCI	CCNGG	4 cut(s)	16, 108, 281, 441
BstSFI	CTRYAG	2 cut(s)	554, 1110
BstUI	CGCG	3 cut(s)	131, 186, 401
BstV1I	GCAGC	1 cut(s)	298
BstV2I	GAAGAC	2 cut(s)	642, 1407
BstX2I	RGATCY	2 cut(s)	657, 1078
BstYI	RGATCY	2 cut(s)	657, 1078
BstZI	CGGCCG	1 cut(s)	402
BsuI	GTATCC	1 cut(s)	1284
BtgI	CCRYGG	4 cut(s)	232, 244, 405, 427
BtsCI	GGATG	3 cut(s)	1151, 1363, 1498
BtsI	GCAGTG	2 cut(s)	551, 1424
BtsIMutI	CAGTG	5 cut(s)	551, 960, 1424, 1516, 1537
Cac8I	GCNNGC	4 cut(s)	124, 270, 1008, 1245
CfoI	GCGC	3 cut(s)	188, 401, 828
Cfr13I	GGNCC	6 cut(s)	20, 68, 84, 163, 307, 323
CseI	GACGC	2 cut(s)	160, 435
Csp6I	GTAC	3 cut(s)	951, 957, 998
CviAII	CATG	7 cut(s)	233, 608, 691, 841, 992, 1235, 1397
CviQI	GTAC	3 cut(s)	951, 957, 998
DdeI	CTNAG	4 cut(s)	411, 546, 667, 1529
DpnI	GATC	7 cut(s)	60, 483, 606, 659, 813, 1080, 1485
DpnII	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
DraIII	CACNNNGTG	1 cut(s)	1532
EaeI	YGGCCR	6 cut(s)	132, 181, 196, 235, 402, 1241
EagI	CGGCCG	1 cut(s)	402
Eam1104I	CTCTTC	1 cut(s)	1213
EarI	CTCTTC	1 cut(s)	1213
EclXI	CGGCCG	1 cut(s)	402
Eco130I	CCWWGG	6 cut(s)	87, 166, 193, 232, 303, 349
Eco47I	GGWCC	3 cut(s)	68, 163, 323
Eco52I	CGGCCG	1 cut(s)	402
Eco57I	CTGAAG	1 cut(s)	986
EcoRII	CCWGG	4 cut(s)	16, 108, 281, 441
EcoT14I	CCWWGG	6 cut(s)	87, 166, 193, 232, 303, 349
EcoT22I	ATGCAT	2 cut(s)	613, 1236
ErhI	CCWWGG	6 cut(s)	87, 166, 193, 232, 303, 349
Esp3I	CGTCTC	1 cut(s)	178
FaeI	CATG	7 cut(s)	236, 611, 694, 844, 995, 1238, 1400
FaqI	GGGAC	2 cut(s)	527, 734
FatI	CATG	7 cut(s)	232, 607, 690, 840, 991, 1234, 1396
FauI	CCCGC	1 cut(s)	1134
FbaI	TGATCA	1 cut(s)	58
FokI	GGATG	3 cut(s)	1158, 1370, 1505
FspBI	CTAG	1 cut(s)	1268
GlaI	GCGC	3 cut(s)	187, 400, 827
HapII	CCGG	2 cut(s)	578, 1487
HgaI	GACGC	2 cut(s)	160, 435
HhaI	GCGC	3 cut(s)	188, 401, 828
Hin1I	GRCGYC	2 cut(s)	171, 446
Hin1II	CATG	7 cut(s)	236, 611, 694, 844, 995, 1238, 1400
Hin6I	GCGC	3 cut(s)	186, 399, 826
HinP1I	GCGC	3 cut(s)	186, 399, 826
HindIII	AAGCTT	2 cut(s)	778, 833
HinfI	GANTC	5 cut(s)	336, 562, 673, 977, 1421
HpaII	CCGG	2 cut(s)	578, 1487
HphI	GGTGA	2 cut(s)	59, 383
Hpy166II	GTNNAC	3 cut(s)	71, 323, 1064
Hpy188I	TCNGA	6 cut(s)	220, 414, 481, 501, 901, 1439
Hpy188III	TCNNGA	5 cut(s)	566, 727, 1015, 1418, 1487
Hpy8I	GTNNAC	3 cut(s)	71, 323, 1064
Hpy99I	CGWCG	1 cut(s)	15
HpyAV	CCTTC	3 cut(s)	647, 1258, 1534
HpyCH4III	ACNGT	4 cut(s)	523, 1409, 1446, 1511
HpyF3I	CTNAG	4 cut(s)	411, 546, 667, 1529
Hsp92I	GRCGYC	2 cut(s)	171, 446
Hsp92II	CATG	7 cut(s)	236, 611, 694, 844, 995, 1238, 1400
HspAI	GCGC	3 cut(s)	186, 399, 826
Kpn2I	TCCGGA	1 cut(s)	1486
KpnI	GGTACC	1 cut(s)	960
Ksp22I	TGATCA	1 cut(s)	58
Kzo9I	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
LmnI	GCTCC	1 cut(s)	1325
Lsp1109I	GCAGC	1 cut(s)	298
LweI	GCATC	8 cut(s)	139, 620, 722, 726, 892, 1175, 1438, 1486
MaeI	CTAG	1 cut(s)	1268
MaeIII	GTNAC	2 cut(s)	470, 1422
MalI	GATC	7 cut(s)	60, 483, 606, 659, 813, 1080, 1485
MbiI	CCGCTC	1 cut(s)	204
MboI	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
MfeI	CAATTG	3 cut(s)	594, 615, 630
MflI	RGATCY	2 cut(s)	657, 1078
MhlI	GDGCHC	1 cut(s)	496
MlsI	TGGCCA	1 cut(s)	1243
MluCI	AATT	4 cut(s)	594, 615, 630, 912
MluNI	TGGCCA	1 cut(s)	1243
MlyI	GAGTC	2 cut(s)	682, 1430
MmeI	TCCRAC	2 cut(s)	1160, 1234
Mox20I	TGGCCA	1 cut(s)	1243
Mph1103I	ATGCAT	2 cut(s)	613, 1236
MroI	TCCGGA	1 cut(s)	1486
MscI	TGGCCA	1 cut(s)	1243
MseI	TTAA	2 cut(s)	141, 915
MslI	CAYNNNNRTG	1 cut(s)	521
Msp20I	TGGCCA	1 cut(s)	1243
MspI	CCGG	2 cut(s)	578, 1487
MspR9I	CCNGG	4 cut(s)	18, 110, 283, 443
MunI	CAATTG	3 cut(s)	594, 615, 630
MvaI	CCWGG	4 cut(s)	18, 110, 283, 443
MvnI	CGCG	3 cut(s)	131, 186, 401
NcoI	CCATGG	1 cut(s)	232
NdeII	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
NlaIII	CATG	7 cut(s)	236, 611, 694, 844, 995, 1238, 1400
NlaIV	GGNNCC	4 cut(s)	6, 164, 659, 958
NmeAIII	GCCGAG	1 cut(s)	354
NmuCI	GTSAC	1 cut(s)	1422
NsiI	ATGCAT	2 cut(s)	613, 1236
NspI	RCATGY	2 cut(s)	995, 1238
OliI	CACNNNNGTG	1 cut(s)	521
PcsI	WCGNNNNNNNCGW	1 cut(s)	1325
PfeI	GAWTC	3 cut(s)	336, 562, 977
PleI	GAGTC	2 cut(s)	681, 1429
PpsI	GAGTC	2 cut(s)	681, 1429
PshAI	GACNNNNGTC	1 cut(s)	161
PsiI	TTATAA	1 cut(s)	1286
Psp6I	CCWGG	4 cut(s)	16, 108, 281, 441
PspGI	CCWGG	4 cut(s)	16, 108, 281, 441
PspN4I	GGNNCC	4 cut(s)	6, 164, 659, 958
PspPI	GGNCC	6 cut(s)	20, 68, 84, 163, 307, 323
PstI	CTGCAG	1 cut(s)	558
PsuI	RGATCY	2 cut(s)	657, 1078
RsaI	GTAC	3 cut(s)	952, 958, 999
RsaNI	GTAC	3 cut(s)	951, 957, 998
RseI	CAYNNNNRTG	1 cut(s)	521
SaqAI	TTAA	2 cut(s)	141, 915
Sau3AI	GATC	7 cut(s)	58, 481, 604, 657, 811, 1078, 1483
Sau96I	GGNCC	6 cut(s)	20, 68, 84, 163, 307, 323
ScaI	AGTACT	1 cut(s)	952
SchI	GAGTC	2 cut(s)	682, 1430
ScrFI	CCNGG	4 cut(s)	18, 110, 283, 443
SduI	GDGCHC	1 cut(s)	496
SfaNI	GCATC	8 cut(s)	139, 620, 722, 726, 892, 1175, 1438, 1486
SfcI	CTRYAG	2 cut(s)	554, 1110
SinI	GGWCC	3 cut(s)	68, 163, 323
SmiMI	CAYNNNNRTG	1 cut(s)	521
SmlI	CTYRAG	3 cut(s)	727, 1213, 1418
SmoI	CTYRAG	3 cut(s)	727, 1213, 1418
Sse9I	AATT	4 cut(s)	594, 615, 630, 912
SspMI	CTAG	1 cut(s)	1268
StyD4I	CCNGG	4 cut(s)	16, 108, 281, 441
StyI	CCWWGG	6 cut(s)	87, 166, 193, 232, 303, 349
TaaI	ACNGT	4 cut(s)	523, 1409, 1446, 1511
TaqI	TCGA	1 cut(s)	676
TasI	AATT	4 cut(s)	594, 615, 630, 912
TatI	WGTACW	1 cut(s)	950
TfiI	GAWTC	3 cut(s)	336, 562, 977
Tru1I	TTAA	2 cut(s)	141, 915
Tru9I	TTAA	2 cut(s)	141, 915
TscAI	CASTG	5 cut(s)	558, 967, 1431, 1516, 1537
TseFI	GTSAC	1 cut(s)	1422
TseI	GCWGC	1 cut(s)	286
Tsp45I	GTSAC	1 cut(s)	1422
TspDTI	ATGAA	6 cut(s)	522, 575, 663, 707, 987, 1413
TspRI	CASTG	5 cut(s)	558, 967, 1431, 1516, 1537
VpaK11BI	GGWCC	3 cut(s)	68, 163, 323
XceI	RCATGY	2 cut(s)	995, 1238
XcmI	CCANNNNNNNNNTGG	2 cut(s)	78, 317
XspI	CTAG	1 cut(s)	1268
ZrmI	AGTACT	1 cut(s)	952
Zsp2I	ATGCAT	2 cut(s)	613, 1236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.