RLG00000011726

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008988
Physical Location & Seq
Forward (+)
12217917 .. 12218414
498 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011726

Sequence Viewer

Length: 498 bp
ATGGCGGTGGCTTCGATCAAAGAGGCTGAAATCCGGCCACTGTTTGAGCGTCTGTTCAAGAGAGTTGAGGAAGCAATGCAGAGTTGTACCGAGTTCGAATCCCTCATCGGAAATTCCAAGTCCACGCTTGACCCTCTCCAACCATGGATCAAAGGAGAACGCAAGGGACTTGAGGACCTCGTGGACAAAGAATGGCTGGTTGACAGGGGCATAAAGGTAGTAGGCAAGTTCTCCAACGTTCAGATATGGAAGTACTACTGGCGTAGATATGATTACACAGACCAACTTGTTCAGTTGGACAGACAATTTAAAAAACTAATAGATTTTCTGATGACAAAGGACTTCGAAACTTGTGATTGGCGGCAACAAATCCAACCGGTAGATGAAATGGACAGGAGACACTCCGCTATAGCAGAGAAGTTAACGCAGCTTAGTAGAGAGAAACTTTCGGAGATGTTAGTGAAGCAGGAAGGTTTTTCGAGGCCTTGTTGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

166

Amino Acids

19.71

Weight (kDa)

6.75

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 14 - 112 1.8e-06 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 361, 405
AclI AACGTT 1 cut(s) 237
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 35
AcsI RAATTY 1 cut(s) 112
AfaI GTAC 2 cut(s) 88, 254
AgeI ACCGGT 1 cut(s) 376
AgsI TTSAA 1 cut(s) 58
AluBI AGCT 1 cut(s) 430
AluI AGCT 1 cut(s) 430
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 1 cut(s) 155
AoxI GGCC 2 cut(s) 35, 482
ApeKI GCWGC 1 cut(s) 427
ApoI RAATTY 1 cut(s) 112
AsiGI ACCGGT 1 cut(s) 376
AspS9I GGNCC 1 cut(s) 175
AsuII TTCGAA 2 cut(s) 96, 345
AvaII GGWCC 1 cut(s) 175
BauI CACGAG 1 cut(s) 179
BbvI GCAGC 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 391
BfmI CTRYAG 1 cut(s) 408
BisI GCNGC 2 cut(s) 362, 428
BlsI GCNGC 2 cut(s) 363, 429
BmcAI AGTACT 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
Bpu14I TTCGAA 2 cut(s) 96, 345
BpuEI CTTGAG 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 143
BsaWI WCCGGW 1 cut(s) 376
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 143
BseMI GCAATG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 439
BshFI GGCC 2 cut(s) 37, 484
BshTI ACCGGT 1 cut(s) 376
BsiSI CCGG 2 cut(s) 34, 377
BslFI GGGAC 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 2 cut(s) 37, 484
Bsp119I TTCGAA 2 cut(s) 96, 345
Bsp143I GATC 2 cut(s) 15, 147
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 3 cut(s) 5, 361, 405
BspANI GGCC 2 cut(s) 37, 484
BspPI GGATC 1 cut(s) 155
BspT104I TTCGAA 2 cut(s) 96, 345
BsrDI GCAATG 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 376
BsrI ACTGG 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 2 cut(s) 15, 147
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 1 cut(s) 42
BstBI TTCGAA 2 cut(s) 96, 345
BstDEI CTNAG 1 cut(s) 431
BstDSI CCRYGG 1 cut(s) 143
BstKTI GATC 2 cut(s) 18, 150
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 2 cut(s) 15, 147
BstSFI CTRYAG 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 439
BsuRI GGCC 2 cut(s) 37, 484
BtgI CCRYGG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 376
Cfr13I GGNCC 1 cut(s) 175
CseI GACGC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 87, 253
CspAI ACCGGT 1 cut(s) 376
CviAII CATG 1 cut(s) 144
CviJI RGCY 6 cut(s) 11, 26, 37, 196, 430, 484
CviKI_1 RGCY 6 cut(s) 11, 26, 37, 196, 430, 484
CviQI GTAC 2 cut(s) 87, 253
DdeI CTNAG 1 cut(s) 431
DpnI GATC 2 cut(s) 17, 149
DpnII GATC 2 cut(s) 15, 147
DraI TTTAAA 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 143
Eco147I AGGCCT 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 1 cut(s) 147
FaiI YATR 5 cut(s) 145, 212, 247, 270, 410
FaqI GGGAC 1 cut(s) 180
FatI CATG 1 cut(s) 143
Fnu4HI GCNGC 2 cut(s) 362, 428
Fsp4HI GCNGC 2 cut(s) 362, 428
GluI GCNGC 2 cut(s) 362, 428
HaeIII GGCC 2 cut(s) 37, 484
HapII CCGG 2 cut(s) 34, 377
HgaI GACGC 1 cut(s) 38
Hin1II CATG 1 cut(s) 147
HincII GTYRAC 2 cut(s) 202, 423
HindII GTYRAC 2 cut(s) 202, 423
HinfI GANTC 1 cut(s) 98
HpaI GTTAAC 1 cut(s) 423
HpaII CCGG 2 cut(s) 34, 377
Hpy166II GTNNAC 4 cut(s) 123, 184, 202, 423
Hpy188I TCNGA 4 cut(s) 110, 243, 330, 451
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 4 cut(s) 123, 184, 202, 423
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 79
HpyF3I CTNAG 1 cut(s) 431
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 1 cut(s) 147
KspAI GTTAAC 1 cut(s) 423
Kzo9I GATC 2 cut(s) 15, 147
LpnPI CCDG 7 cut(s) 47, 182, 190, 244, 379, 390, 452
Lsp1109I GCAGC 1 cut(s) 439
MaeII ACGT 1 cut(s) 237
MalI GATC 2 cut(s) 17, 149
MboI GATC 2 cut(s) 15, 147
MluCI AATT 2 cut(s) 112, 305
MmeI TCCRAC 4 cut(s) 163, 258, 276, 397
MnlI CCTC 7 cut(s) 16, 61, 113, 144, 166, 188, 474
MseI TTAA 3 cut(s) 309, 422, 496
MspI CCGG 2 cut(s) 34, 377
NcoI CCATGG 1 cut(s) 143
NdeII GATC 2 cut(s) 15, 147
NlaIII CATG 1 cut(s) 147
NspV TTCGAA 2 cut(s) 96, 345
PceI AGGCCT 1 cut(s) 484
PfeI GAWTC 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 376
PkrI GCNGC 2 cut(s) 363, 429
PpuMI RGGWCCY 1 cut(s) 175
Psp1406I AACGTT 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 175
PspPI GGNCC 1 cut(s) 175
PspPPI RGGWCCY 1 cut(s) 175
RsaI GTAC 2 cut(s) 88, 254
RsaNI GTAC 2 cut(s) 87, 253
SaqAI TTAA 3 cut(s) 309, 422, 496
SatI GCNGC 2 cut(s) 362, 428
Sau3AI GATC 2 cut(s) 15, 147
Sau96I GGNCC 1 cut(s) 175
ScaI AGTACT 1 cut(s) 254
SetI ASST 5 cut(s) 180, 219, 240, 432, 475
SfcI CTRYAG 1 cut(s) 408
SfuI TTCGAA 2 cut(s) 96, 345
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 2 cut(s) 112, 305
SseBI AGGCCT 1 cut(s) 484
SsiI CCGC 3 cut(s) 5, 361, 405
StuI AGGCCT 1 cut(s) 484
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 1 cut(s) 240
TaqI TCGA 4 cut(s) 14, 96, 345, 479
TasI AATT 2 cut(s) 112, 305
TatI WGTACW 1 cut(s) 252
TauI GCSGC 1 cut(s) 364
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 3 cut(s) 309, 422, 496
Tru9I TTAA 3 cut(s) 309, 422, 496
TscAI CASTG 1 cut(s) 45
TseI GCWGC 1 cut(s) 427
TspDTI ATGAA 1 cut(s) 399
TspRI CASTG 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 1 cut(s) 112
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.