Rh6DG371300
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008988
Physical Location & Seq
Reverse (-)
56687230 .. 56687700
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG371300.1

Sequence Viewer

Length: 471 bp
ATGGATATGGTGGAGACCTTGGAGAAGCTCTTATTATCATCAAGTAAAGTAGAGTTGCCTCTCAAGCAATCTGAAGAGCTGTTAGTTACAACGTTTCCCAGGTTGCTTGATCTTGTTATAGAGGAGGAAAAGAGTACCACTACGATGTTTAAACCCCTCTTTGGAGATCTAAAATCCACACTGCAGTCCTTAAAACCCTTCGCCGAGAAGATAGAGGTATTGGATCTCGCAGAGGAGGAAGTAGAGGATATTATAGCACTAATGGAGAAGGGTTTCGAGCTTGTCCCAAGTTCTCCTGGTGTCGTGCTGCGTAGCAGCTACTACAAAAAGCATGTATACATGAACCAACTTCTTGAACTGAATCATTCTATTCAGAAACAATTGAGTATACTGAGAAAGCAAATGGCAAGTGATCTAAAGCAAGGAATAGGTTTCGTCAAGAAATATAGAGGCGTTGCTCAGCAAGCTTGA

Protein Analysis

156

Amino Acids

17.76

Weight (kDa)

5.52

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 32 - 130 1.1e-14 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 336, 388
AclI AACGTT 1 cut(s) 92
AclWI GGATC 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 1 cut(s) 356
AjnI CCWGG 2 cut(s) 98, 295
AluBI AGCT 5 cut(s) 28, 79, 280, 318, 467
AluI AGCT 5 cut(s) 28, 79, 280, 318, 467
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 1 cut(s) 231
ApeKI GCWGC 2 cut(s) 307, 315
Asp700I GAANNNNTTC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 294, 327
BciT130I CCWGG 2 cut(s) 100, 297
BcoDI GTCTC 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 182
BglII AGATCT 1 cut(s) 166
BisI GCNGC 2 cut(s) 308, 316
BlpI GCTNAGC 1 cut(s) 459
BlsI GCNGC 2 cut(s) 309, 317
Bme1390I CCNGG 2 cut(s) 100, 297
BmrFI CCNGG 2 cut(s) 100, 297
Bpu1102I GCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 18, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 161
BseBI CCWGG 2 cut(s) 100, 297
BseDI CCNNGG 2 cut(s) 18, 98
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 383
BseRI GAGGAG 2 cut(s) 137, 248
BseXI GCAGC 2 cut(s) 294, 327
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 269
Bso31I GGTCTC 1 cut(s) 8
Bsp143I GATC 4 cut(s) 109, 166, 223, 412
Bsp1720I GCTNAGC 1 cut(s) 459
BspCNI CTCAG 1 cut(s) 384
BspMAI CTGCAG 1 cut(s) 186
BspPI GGATC 1 cut(s) 231
BspQI GCTCTTC 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 8
BssECI CCNNGG 2 cut(s) 18, 98
BssMI GATC 4 cut(s) 109, 166, 223, 412
BssNAI GTATAC 2 cut(s) 337, 389
BssT1I CCWWGG 1 cut(s) 18
Bst1107I GTATAC 2 cut(s) 337, 389
Bst2UI CCWGG 2 cut(s) 100, 297
Bst6I CTCTTC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 2 cut(s) 392, 459
BstKTI GATC 4 cut(s) 112, 169, 226, 415
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 4 cut(s) 109, 166, 223, 412
BstMWI GCNNNNNNNGC 2 cut(s) 64, 464
BstNI CCWGG 2 cut(s) 100, 297
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 98, 295
BstSFI CTRYAG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 294, 327
BstX2I RGATCY 2 cut(s) 166, 223
BstYI RGATCY 2 cut(s) 166, 223
BstZ17I GTATAC 2 cut(s) 337, 389
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 465
Csp6I GTAC 1 cut(s) 135
CviAII CATG 2 cut(s) 332, 340
CviJI RGCY 5 cut(s) 28, 79, 280, 318, 467
CviKI_1 RGCY 5 cut(s) 28, 79, 280, 318, 467
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 2 cut(s) 392, 459
DpnI GATC 4 cut(s) 111, 168, 225, 414
DpnII GATC 4 cut(s) 109, 166, 223, 412
DraI TTTAAA 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 93
EcoRII CCWGG 2 cut(s) 98, 295
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 2 cut(s) 335, 343
FaiI YATR 8 cut(s) 8, 119, 254, 333, 337, 341, 389, 447
FaqI GGGAC 1 cut(s) 269
FatI CATG 2 cut(s) 331, 339
FblI GTMKAC 2 cut(s) 336, 388
Fnu4HI GCNGC 2 cut(s) 308, 316
Fsp4HI GCNGC 2 cut(s) 308, 316
GluI GCNGC 2 cut(s) 308, 316
Hin1II CATG 2 cut(s) 335, 343
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 337, 389
Hpy188I TCNGA 2 cut(s) 73, 375
Hpy188III TCNNGA 2 cut(s) 353, 439
Hpy8I GTNNAC 2 cut(s) 337, 389
HpyAV CCTTC 2 cut(s) 208, 262
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 1 cut(s) 184
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 464
HpyF3I CTNAG 2 cut(s) 392, 459
HpySE526I ACGT 1 cut(s) 92
Hsp92II CATG 2 cut(s) 335, 343
Kzo9I GATC 4 cut(s) 109, 166, 223, 412
LguI GCTCTTC 1 cut(s) 69
LpnPI CCDG 4 cut(s) 85, 112, 282, 309
Lsp1109I GCAGC 2 cut(s) 294, 327
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 85
MalI GATC 4 cut(s) 111, 168, 225, 414
MboI GATC 4 cut(s) 109, 166, 223, 412
MboII GAAGA 2 cut(s) 86, 220
MfeI CAATTG 1 cut(s) 380
MflI RGATCY 2 cut(s) 166, 223
MluCI AATT 1 cut(s) 380
MnlI CCTC 9 cut(s) 69, 115, 118, 167, 208, 226, 229, 238, 443
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 2 cut(s) 150, 191
MslI CAYNNNNRTG 1 cut(s) 143
MspR9I CCNGG 2 cut(s) 100, 297
MssI GTTTAAAC 1 cut(s) 151
MunI CAATTG 1 cut(s) 380
MvaI CCWGG 2 cut(s) 100, 297
MwoI GCNNNNNNNGC 2 cut(s) 64, 464
NdeII GATC 4 cut(s) 109, 166, 223, 412
NlaIII CATG 2 cut(s) 335, 343
NmeAIII GCCGAG 1 cut(s) 229
NspI RCATGY 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 2 cut(s) 309, 317
PmeI GTTTAAAC 1 cut(s) 151
Psp1406I AACGTT 1 cut(s) 92
Psp6I CCWGG 2 cut(s) 98, 295
PspGI CCWGG 2 cut(s) 98, 295
PstI CTGCAG 1 cut(s) 186
PsuI RGATCY 2 cut(s) 166, 223
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 143
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 150, 191
SatI GCNGC 2 cut(s) 308, 316
Sau3AI GATC 4 cut(s) 109, 166, 223, 412
ScrFI CCNGG 2 cut(s) 100, 297
SfcI CTRYAG 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 98, 295
StyI CCWWGG 1 cut(s) 18
TaiI ACGT 1 cut(s) 95
TaqI TCGA 1 cut(s) 276
TasI AATT 1 cut(s) 380
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 2 cut(s) 150, 191
Tru9I TTAA 2 cut(s) 150, 191
TscAI CASTG 1 cut(s) 186
TseI GCWGC 2 cut(s) 307, 315
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 1 cut(s) 186
XceI RCATGY 1 cut(s) 335
XmiI GTMKAC 2 cut(s) 336, 388
XmnI GAANNNNTTC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.