RLG00000019038

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008182
Physical Location & Seq
Reverse (-)
45172046 .. 45175460
3415 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019038

Sequence Viewer

Length: 537 bp
ATGGCTGGCCTTTCTCTAACCTCAGAGGCGACTTACTCTTCTGGTGGTAATATGAAGCCTTTTGGACCGGAGCGCTACAGCAAGGCTATGGGACCAAGAGGGCAGGATATGGAATTTTCTGGTGGCAGCAACGGTGATCTGCTTGGTGAGAAGGGTGACCCAGCTGCAGTTGTTCATCATGGAACAGTTGGGCATGTGCCTATGGGCAGTCCCATAATTCTGCAACAGGTCCTGACTAATAGTCCCGGAGCTGGAGGTTTTCGTACTAGGGAAGTGTTCCCTTCAGTTGAGGTAAGCCCGACATCATTGGCTATGGTCCCTGTATTGGCCAAGGTAGTGAAAAAGCTTCGAAAGTATAAGCGTAGGGAAAAACTTTTGCAAAATGATCCCAACTTTGTTGGCTCTGAGGATGCAAATACTGATCAGGGGGAGACTGCACATTTGATGGGGAGTGAGGCTATGCAGATGGTGGAGTCTGCTGTTGCAGAGGGTCAGGTGGAGGGTGACGAGTCACCTTCTACTACTAGTTTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

18.73

Weight (kDa)

5.37

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 1 cut(s) 265
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 251, 325
AhdI GACNNNNNGTC 1 cut(s) 240
AhlI ACTAGT 1 cut(s) 524
AluBI AGCT 3 cut(s) 164, 251, 346
AluI AGCT 3 cut(s) 164, 251, 346
Alw26I GTCTC 1 cut(s) 425
AlwI GGATC 1 cut(s) 380
AlwNI CAGNNNCTG 1 cut(s) 232
Aor51HI AGCGCT 1 cut(s) 74
AoxI GGCC 2 cut(s) 7, 327
ApeKI GCWGC 2 cut(s) 126, 164
ApoI RAATTY 1 cut(s) 113
ArsI GACNNNNNNTTYG 2 cut(s) 424, 456
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 4 cut(s) 65, 92, 229, 316
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 5 cut(s) 146, 158, 167, 504, 515
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 4 cut(s) 65, 92, 229, 316
BalI TGGCCA 1 cut(s) 329
BbvI GCAGC 2 cut(s) 138, 151
BccI CCATC 2 cut(s) 439, 460
BclI TGATCA 1 cut(s) 421
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 425
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 2 cut(s) 267, 525
BfmI CTRYAG 2 cut(s) 76, 165
BfoI RGCGCY 1 cut(s) 76
BisI GCNGC 2 cut(s) 127, 165
BlsI GCNGC 2 cut(s) 128, 166
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 4 cut(s) 65, 92, 229, 316
BmeRI GACNNNNNGTC 1 cut(s) 240
BmgT120I GGNCC 4 cut(s) 65, 92, 229, 316
BmiI GGNNCC 2 cut(s) 93, 318
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 1 cut(s) 400
BpmI CTGGAG 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 349
BpuMI CCSGG 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 330
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 325
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 2 cut(s) 251, 325
BseMII CTCAG 2 cut(s) 36, 396
BseXI GCAGC 2 cut(s) 138, 151
BseYI CCCAGC 1 cut(s) 160
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 2 cut(s) 9, 329
BsiSI CCGG 2 cut(s) 68, 246
BslFI GGGAC 4 cut(s) 105, 195, 228, 302
BslI CCNNNNNNNGG 2 cut(s) 251, 325
BsmAI GTCTC 1 cut(s) 425
BsmFI GGGAC 4 cut(s) 105, 195, 228, 302
BsnI GGCC 2 cut(s) 9, 329
Bsp119I TTCGAA 1 cut(s) 349
Bsp143I GATC 3 cut(s) 136, 385, 421
BspANI GGCC 2 cut(s) 9, 329
BspCNI CTCAG 2 cut(s) 35, 397
BspLI GGNNCC 2 cut(s) 93, 318
BspMAI CTGCAG 1 cut(s) 169
BspPI GGATC 1 cut(s) 380
BspT104I TTCGAA 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 3 cut(s) 136, 385, 421
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 134, 187
Bst6I CTCTTC 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 22, 405
BstEII GGTNACC 1 cut(s) 155
BstF5I GGATG 1 cut(s) 415
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 3 cut(s) 139, 388, 424
BstMAI GTCTC 1 cut(s) 425
BstMBI GATC 3 cut(s) 136, 385, 421
BstNSI RCATGY 1 cut(s) 197
BstPI GGTNACC 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 2 cut(s) 76, 165
BstV1I GCAGC 2 cut(s) 138, 151
BsuRI GGCC 2 cut(s) 9, 329
BtsCI GGATG 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 4 cut(s) 65, 92, 229, 316
Csp6I GTAC 1 cut(s) 264
CviAII CATG 2 cut(s) 179, 194
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 2 cut(s) 22, 405
DpnI GATC 3 cut(s) 138, 387, 423
DpnII GATC 3 cut(s) 136, 385, 421
DriI GACNNNNNGTC 1 cut(s) 240
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 43
Eam1105I GACNNNNNGTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 330
Eco47I GGWCC 4 cut(s) 65, 92, 229, 316
Eco47III AGCGCT 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 267
Eco91I GGTNACC 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 2 cut(s) 182, 197
FaqI GGGAC 4 cut(s) 105, 195, 228, 302
FatI CATG 2 cut(s) 178, 193
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 2 cut(s) 127, 165
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 2 cut(s) 127, 165
FspBI CTAG 2 cut(s) 267, 525
GlaI GCGC 1 cut(s) 74
GluI GCNGC 2 cut(s) 127, 165
GsaI CCCAGC 1 cut(s) 164
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 2 cut(s) 9, 329
HapII CCGG 2 cut(s) 68, 246
HhaI GCGC 1 cut(s) 75
Hin1II CATG 2 cut(s) 182, 197
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 2 cut(s) 473, 509
HpaII CCGG 2 cut(s) 68, 246
HphI GGTGA 5 cut(s) 146, 158, 167, 504, 515
Hpy188I TCNGA 2 cut(s) 25, 406
Hpy188III TCNNGA 1 cut(s) 232
HpyAV CCTTC 3 cut(s) 145, 291, 525
HpyCH4III ACNGT 2 cut(s) 134, 187
HpyCH4V TGCA 7 cut(s) 167, 223, 379, 413, 437, 463, 485
HpyF3I CTNAG 2 cut(s) 22, 405
Hsp92II CATG 2 cut(s) 182, 197
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 3 cut(s) 136, 385, 421
LmnI GCTCC 2 cut(s) 70, 248
Lsp1109I GCAGC 2 cut(s) 138, 151
LweI GCATC 1 cut(s) 400
MaeI CTAG 2 cut(s) 267, 525
MaeIII GTNAC 3 cut(s) 155, 503, 510
MalI GATC 3 cut(s) 138, 387, 423
MboI GATC 3 cut(s) 136, 385, 421
MboII GAAGA 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 2 cut(s) 113, 216
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 2 cut(s) 482, 518
MnlI CCTC 9 cut(s) 19, 31, 92, 248, 283, 400, 448, 481, 493
Mox20I TGGCCA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 329
MseI TTAA 1 cut(s) 535
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 2 cut(s) 68, 246
MspR9I CCNGG 1 cut(s) 246
NciI CCSGG 1 cut(s) 246
NdeII GATC 3 cut(s) 136, 385, 421
NlaIII CATG 2 cut(s) 182, 197
NlaIV GGNNCC 2 cut(s) 93, 318
NmuCI GTSAC 3 cut(s) 155, 503, 510
NspI RCATGY 1 cut(s) 197
NspV TTCGAA 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 2 cut(s) 128, 166
PleI GAGTC 2 cut(s) 481, 517
PpsI GAGTC 2 cut(s) 481, 517
PpuMI RGGWCCY 1 cut(s) 229
Psp5II RGGWCCY 1 cut(s) 229
PspEI GGTNACC 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 93, 318
PspPI GGNCC 4 cut(s) 65, 92, 229, 316
PspPPI RGGWCCY 1 cut(s) 229
PstI CTGCAG 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 535
SatI GCNGC 2 cut(s) 127, 165
Sau3AI GATC 3 cut(s) 136, 385, 421
Sau96I GGNCC 4 cut(s) 65, 92, 229, 316
SchI GAGTC 2 cut(s) 482, 518
ScrFI CCNGG 1 cut(s) 246
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 2 cut(s) 76, 165
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 4 cut(s) 65, 92, 229, 316
SpeI ACTAGT 1 cut(s) 524
Sse9I AATT 2 cut(s) 113, 216
SspMI CTAG 2 cut(s) 267, 525
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 2 cut(s) 134, 187
TaqI TCGA 1 cut(s) 349
TasI AATT 2 cut(s) 113, 216
Tru1I TTAA 1 cut(s) 535
Tru9I TTAA 1 cut(s) 535
TseFI GTSAC 3 cut(s) 155, 503, 510
TseI GCWGC 2 cut(s) 126, 164
Tsp45I GTSAC 3 cut(s) 155, 503, 510
TspDTI ATGAA 2 cut(s) 68, 164
VpaK11BI GGWCC 4 cut(s) 65, 92, 229, 316
XapI RAATTY 1 cut(s) 113
XceI RCATGY 1 cut(s) 197
XspI CTAG 2 cut(s) 267, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.