Rorug04G0365600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008182
Physical Location & Seq
Reverse (-)
53747549 .. 53748418
870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0365600.1

Sequence Viewer

Length: 516 bp
ATGTACTCTAGCATGGCCGAATTGCAGAACGAGCCACTCCCTTCGGCCCCATCGGCGCCACCCCCAAGTCCTCGGATTACACCACCACCATCAGCACCACCTCTTCCTTTTGATTTGAATGATCAGCCCCTCACAACCACCGGCGTTCCGGTCATGCCAGTGACCCTTCCTCATTCCCCAGCCCCTTGGTCCACTGACCTATGCGATTGTTGTGACGATCTCAGCAGTTGTTGCTTAACGTGTTGGTGTCCATGTATAACATTTGGACGCATTGCAGAAATTGTGGACAGGGGATCATCTTCATGTGGAGTGAGTGGAATACTTTACAGTTTGATGCTGTGCTTGATGGGTTGCTCGTGTTTGTATTCGTGCTTCTATCGATCTAGACTGAGAGGGCAGTACTTCTTGGAGGAGAAAAGCCATGCGCAGATTGCTGTGTCCATTTTTGCTGCGAGGTATGCGCTCTGTGCCAAGAGTATCGACACCTTCAGAACCAGGGCTTCGACATGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.51

Weight (kDa)

5.57

Isoelectric Point (pI)

75.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 63 - 156 5.3e-23 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 55
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 15
AcuI CTGAAG 1 cut(s) 472
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 2 cut(s) 5, 401
AflIII ACRYGT 2 cut(s) 239, 506
AgsI TTSAA 1 cut(s) 118
AjnI CCWGG 1 cut(s) 494
AlwI GGATC 1 cut(s) 301
AoxI GGCC 2 cut(s) 15, 45
ApeKI GCWGC 1 cut(s) 449
AspLEI GCGC 3 cut(s) 58, 427, 463
AspS9I GGNCC 2 cut(s) 46, 189
AvaII GGWCC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
BauI CACGAG 1 cut(s) 355
BbvI GCAGC 1 cut(s) 436
BccI CCATC 3 cut(s) 58, 97, 340
BciT130I CCWGG 1 cut(s) 496
BclI TGATCA 1 cut(s) 121
BfaI CTAG 2 cut(s) 9, 384
BfoI RGCGCY 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 496
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 46, 189
BmiI GGNNCC 2 cut(s) 48, 57
BmrFI CCNGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 324
Bsa29I ATCGAT 1 cut(s) 379
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 3 cut(s) 71, 185, 495
BsaWI WCCGGW 1 cut(s) 148
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 1 cut(s) 496
BseCI ATCGAT 1 cut(s) 379
BseDI CCNNGG 3 cut(s) 71, 185, 495
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 235, 380
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 1 cut(s) 436
BseYI CCCAGC 1 cut(s) 178
BshFI GGCC 2 cut(s) 17, 47
BshNI GGYRCC 1 cut(s) 55
BshVI ATCGAT 1 cut(s) 379
BsiSI CCGG 2 cut(s) 141, 149
BsnI GGCC 2 cut(s) 17, 47
Bsp143I GATC 4 cut(s) 121, 217, 293, 380
BspANI GGCC 2 cut(s) 17, 47
BspCNI CTCAG 2 cut(s) 234, 381
BspDI ATCGAT 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 48, 57
BspPI GGATC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 3 cut(s) 71, 185, 495
BssMI GATC 4 cut(s) 121, 217, 293, 380
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 185
Bst2BI CACGAG 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 496
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 1 cut(s) 108
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 221, 389
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 3 cut(s) 58, 427, 463
BstKTI GATC 4 cut(s) 124, 220, 296, 383
BstMBI GATC 4 cut(s) 121, 217, 293, 380
BstMWI GCNNNNNNNGC 6 cut(s) 31, 53, 231, 431, 458, 467
BstNI CCWGG 1 cut(s) 496
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 436
BstXI CCANNNNNNTGG 1 cut(s) 186
Bsu15I ATCGAT 1 cut(s) 379
BsuRI GGCC 2 cut(s) 17, 47
BsuTUI ATCGAT 1 cut(s) 379
BtsIMutI CAGTG 2 cut(s) 165, 192
CfoI GCGC 3 cut(s) 58, 427, 463
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 2 cut(s) 46, 189
ClaI ATCGAT 1 cut(s) 379
CseI GACGC 1 cut(s) 276
Csp6I GTAC 2 cut(s) 4, 400
CviAII CATG 6 cut(s) 13, 154, 252, 303, 422, 507
CviJI RGCY 7 cut(s) 17, 34, 47, 127, 182, 420, 500
CviKI_1 RGCY 7 cut(s) 17, 34, 47, 127, 182, 420, 500
CviQI GTAC 2 cut(s) 4, 400
DdeI CTNAG 2 cut(s) 221, 389
DinI GGCGCC 1 cut(s) 57
DpnI GATC 4 cut(s) 123, 219, 295, 382
DpnII GATC 4 cut(s) 121, 217, 293, 380
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 494
EcoT14I CCWWGG 1 cut(s) 185
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 6 cut(s) 16, 157, 255, 306, 425, 510
FatI CATG 6 cut(s) 12, 153, 251, 302, 421, 506
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 450
Fsp4HI GCNGC 1 cut(s) 450
FspBI CTAG 2 cut(s) 9, 384
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 3 cut(s) 57, 426, 462
GluI GCNGC 1 cut(s) 450
GsaI CCCAGC 1 cut(s) 182
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 2 cut(s) 17, 47
HapII CCGG 2 cut(s) 141, 149
HgaI GACGC 1 cut(s) 276
HhaI GCGC 3 cut(s) 58, 427, 463
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 6 cut(s) 16, 157, 255, 306, 425, 510
Hin6I GCGC 3 cut(s) 56, 425, 461
HinP1I GCGC 3 cut(s) 56, 425, 461
HpaII CCGG 2 cut(s) 141, 149
Hpy166II GTNNAC 2 cut(s) 192, 286
Hpy188I TCNGA 2 cut(s) 75, 491
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 2 cut(s) 192, 286
HpyAV CCTTC 3 cut(s) 51, 176, 496
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 2 cut(s) 25, 275
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 53, 231, 431, 458, 467
HpyF3I CTNAG 2 cut(s) 221, 389
HpySE526I ACGT 1 cut(s) 239
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 6 cut(s) 16, 157, 255, 306, 425, 510
HspAI GCGC 3 cut(s) 56, 425, 461
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 4 cut(s) 121, 217, 293, 380
LpnPI CCDG 7 cut(s) 154, 162, 171, 192, 274, 481, 508
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 1 cut(s) 324
MaeI CTAG 2 cut(s) 9, 384
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 2 cut(s) 160, 212
MalI GATC 4 cut(s) 123, 219, 295, 382
MboI GATC 4 cut(s) 121, 217, 293, 380
MboII GAAGA 2 cut(s) 95, 291
MluCI AATT 2 cut(s) 20, 279
Mly113I GGCGCC 1 cut(s) 56
MnlI CCTC 7 cut(s) 81, 111, 140, 180, 386, 403, 447
MseI TTAA 1 cut(s) 236
MslI CAYNNNNRTG 2 cut(s) 158, 301
MspI CCGG 2 cut(s) 141, 149
MspR9I CCNGG 1 cut(s) 496
MvaI CCWGG 1 cut(s) 496
MwoI GCNNNNNNNGC 6 cut(s) 31, 53, 231, 431, 458, 467
NarI GGCGCC 1 cut(s) 56
NdeII GATC 4 cut(s) 121, 217, 293, 380
NlaIII CATG 6 cut(s) 16, 157, 255, 306, 425, 510
NlaIV GGNNCC 2 cut(s) 48, 57
NmuCI GTSAC 2 cut(s) 160, 212
NsbI TGCGCA 1 cut(s) 426
NspI RCATGY 1 cut(s) 510
PciI ACATGT 1 cut(s) 506
PkrI GCNGC 1 cut(s) 451
PluTI GGCGCC 1 cut(s) 59
PscI ACATGT 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 494
PspFI CCCAGC 1 cut(s) 178
PspGI CCWGG 1 cut(s) 494
PspN4I GGNNCC 2 cut(s) 48, 57
PspPI GGNCC 2 cut(s) 46, 189
RsaI GTAC 2 cut(s) 5, 401
RsaNI GTAC 2 cut(s) 4, 400
RseI CAYNNNNRTG 2 cut(s) 158, 301
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 4 cut(s) 121, 217, 293, 380
Sau96I GGNCC 2 cut(s) 46, 189
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 496
SetI ASST 5 cut(s) 103, 201, 242, 458, 488
SfaNI GCATC 1 cut(s) 324
SfoI GGCGCC 1 cut(s) 57
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 1 cut(s) 189
SmiMI CAYNNNNRTG 2 cut(s) 158, 301
Sse9I AATT 2 cut(s) 20, 279
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 2 cut(s) 9, 384
StyD4I CCNGG 1 cut(s) 494
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 1 cut(s) 242
TaqI TCGA 3 cut(s) 379, 480, 503
TasI AATT 2 cut(s) 20, 279
TatI WGTACW 2 cut(s) 3, 399
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 2 cut(s) 165, 199
TseFI GTSAC 2 cut(s) 160, 212
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 2 cut(s) 160, 212
TspDTI ATGAA 1 cut(s) 291
TspRI CASTG 2 cut(s) 165, 199
VpaK11BI GGWCC 1 cut(s) 189
XbaI TCTAGA 1 cut(s) 383
XceI RCATGY 1 cut(s) 510
XspI CTAG 2 cut(s) 9, 384
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.