RLG00000020175

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004252
Physical Location & Seq
Forward (+)
61998430 .. 61999385
956 bp
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UTR
Exon/CDS
Intron
RLM00000020175

Sequence Viewer

Length: 471 bp
ATGTTGCTTTTGACGCTCCCGCAGGCAATAGGTTGGCTGCAGTTGTCATATTCCACTGTATCATTGGCACAGAGACTGAATGGGAGGTTGACTGAGAAAAAGGAAGTGATCAAAAGGAAAGGCAGAAAGGAAGGGCGACTAAAGGACGACGGCGTGTGGCGTATGACTGCACAGGCGGCTGAGGTCTGGTGTACATGTGCGGTGGCGATCCTAGATACAAGGGTGGTGAGCGGCCTTGCGGCGTTGCAGTTTCGATATGGTCCTAGTGGCTCTGTCCTAGCGGCGCTGATGGCTCAATCATGGCAGAGGAAGATCGAGATCCGACCGGAGGGTGGGGCAACAGGCGTGGCGGTGGTTTTAAGGCAGATTACCAGCGACGTGGAGGCTGCACATGCGCTCAGGCTGGAGGTCTCGACGGCAAGAGCGGATGCGGGCAGGCGGAGAGCAGCGACGGCAGTTCGGGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

16.91

Weight (kDa)

10.64

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 231, 425
AclWI GGATC 2 cut(s) 202, 313
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 328, 332
AflIII ACRYGT 1 cut(s) 194
AjiI CACGTC 1 cut(s) 379
Alw26I GTCTC 2 cut(s) 67, 415
AlwI GGATC 2 cut(s) 202, 313
AlwNI CAGNNNCTG 1 cut(s) 76
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 3 cut(s) 37, 386, 446
AspLEI GCGC 2 cut(s) 286, 397
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 238
AvaII GGWCC 1 cut(s) 260
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 3 cut(s) 24, 373, 458
BccI CCATC 1 cut(s) 283
BceAI ACGGC 3 cut(s) 166, 432, 468
BclI TGATCA 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 67, 415
BfaI CTAG 3 cut(s) 212, 264, 278
BfmI CTRYAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 287
BisI GCNGC 7 cut(s) 38, 177, 232, 240, 282, 387, 447
BlsI GCNGC 7 cut(s) 39, 178, 233, 241, 283, 388, 448
Bme18I GGWCC 1 cut(s) 260
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 260
BmsI GCATC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 425
Bpu10I CCTNAGC 2 cut(s) 180, 398
BsaBI GATNNNNATC 1 cut(s) 317
BsaI GGTCTC 1 cut(s) 415
BsaWI WCCGGW 1 cut(s) 325
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 332
Bse8I GATNNNNATC 1 cut(s) 317
BseGI GGATG 1 cut(s) 433
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 2 cut(s) 328, 332
BseMII CTCAG 3 cut(s) 84, 171, 412
BseXI GCAGC 3 cut(s) 24, 373, 458
BsgI GTGCAG 2 cut(s) 153, 372
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 326
BsiSI CCGG 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 328, 332
BsmAI GTCTC 2 cut(s) 67, 415
BsnI GGCC 1 cut(s) 234
Bso31I GGTCTC 1 cut(s) 415
Bsp1407I TGTACA 1 cut(s) 191
Bsp143I GATC 4 cut(s) 108, 207, 312, 318
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 3 cut(s) 85, 172, 411
BspMAI CTGCAG 1 cut(s) 42
BspPI GGATC 2 cut(s) 202, 313
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 2 cut(s) 231, 425
BsrGI TGTACA 1 cut(s) 191
BssMI GATC 4 cut(s) 108, 207, 312, 318
Bst4CI ACNGT 1 cut(s) 58
BstAUI TGTACA 1 cut(s) 191
BstC8I GCNNGC 4 cut(s) 24, 433, 437, 466
BstDEI CTNAG 3 cut(s) 93, 180, 398
BstF5I GGATG 1 cut(s) 433
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 2 cut(s) 286, 397
BstKTI GATC 4 cut(s) 111, 210, 315, 321
BstMAI GTCTC 2 cut(s) 67, 415
BstMBI GATC 4 cut(s) 108, 207, 312, 318
BstMCI CGRYCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 6 cut(s) 13, 176, 290, 392, 452, 461
BstNSI RCATGY 2 cut(s) 198, 395
BstSFI CTRYAG 1 cut(s) 38
BstV1I GCAGC 3 cut(s) 24, 373, 458
BstX2I RGATCY 1 cut(s) 318
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 1 cut(s) 318
BsuRI GGCC 1 cut(s) 234
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 4 cut(s) 24, 433, 437, 466
CaiI CAGNNNCTG 1 cut(s) 76
CfoI GCGC 2 cut(s) 286, 397
Cfr13I GGNCC 1 cut(s) 260
CseI GACGC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 192
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 3 cut(s) 195, 300, 392
CviJI RGCY 8 cut(s) 37, 179, 234, 270, 293, 386, 403, 468
CviKI_1 RGCY 8 cut(s) 37, 179, 234, 270, 293, 386, 403, 468
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 3 cut(s) 93, 180, 398
DpnI GATC 4 cut(s) 110, 209, 314, 320
DpnII GATC 4 cut(s) 108, 207, 312, 318
EciI GGCGGA 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 260
FaeI CATG 3 cut(s) 198, 303, 395
FaiI YATR 6 cut(s) 49, 164, 196, 258, 301, 393
FatI CATG 3 cut(s) 194, 299, 391
FauI CCCGC 2 cut(s) 27, 424
FbaI TGATCA 1 cut(s) 108
Fnu4HI GCNGC 7 cut(s) 38, 177, 232, 240, 282, 387, 447
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 7 cut(s) 38, 177, 232, 240, 282, 387, 447
FspBI CTAG 3 cut(s) 212, 264, 278
GlaI GCGC 2 cut(s) 285, 396
GluI GCNGC 7 cut(s) 38, 177, 232, 240, 282, 387, 447
GsuI CTGGAG 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 326
HgaI GACGC 1 cut(s) 22
HhaI GCGC 2 cut(s) 286, 397
Hin1II CATG 3 cut(s) 198, 303, 395
Hin6I GCGC 2 cut(s) 284, 395
HinP1I GCGC 2 cut(s) 284, 395
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HpaII CCGG 1 cut(s) 326
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 90, 192
Hpy188I TCNGA 1 cut(s) 323
Hpy188III TCNNGA 2 cut(s) 316, 412
Hpy8I GTNNAC 2 cut(s) 90, 192
Hpy99I CGWCG 4 cut(s) 152, 380, 418, 454
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 4 cut(s) 40, 170, 247, 389
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 176, 290, 392, 452, 461
HpyF3I CTNAG 3 cut(s) 93, 180, 398
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 3 cut(s) 198, 303, 395
HspAI GCGC 2 cut(s) 284, 395
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 4 cut(s) 108, 207, 312, 318
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 3 cut(s) 24, 373, 458
LweI GCATC 1 cut(s) 418
MaeI CTAG 3 cut(s) 212, 264, 278
MaeII ACGT 1 cut(s) 378
MalI GATC 4 cut(s) 110, 209, 314, 320
MbiI CCGCTC 2 cut(s) 231, 425
MboI GATC 4 cut(s) 108, 207, 312, 318
MboII GAAGA 1 cut(s) 322
MflI RGATCY 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 6 cut(s) 78, 175, 300, 322, 376, 400
MseI TTAA 1 cut(s) 359
MspI CCGG 1 cut(s) 326
MwoI GCNNNNNNNGC 6 cut(s) 13, 176, 290, 392, 452, 461
NdeII GATC 4 cut(s) 108, 207, 312, 318
NlaIII CATG 3 cut(s) 198, 303, 395
NspI RCATGY 2 cut(s) 198, 395
PciI ACATGT 1 cut(s) 194
PkrI GCNGC 7 cut(s) 39, 178, 233, 241, 283, 388, 448
PscI ACATGT 1 cut(s) 194
PspPI GGNCC 1 cut(s) 260
PstI CTGCAG 1 cut(s) 42
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 318
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
SaqAI TTAA 1 cut(s) 359
SatI GCNGC 7 cut(s) 38, 177, 232, 240, 282, 387, 447
Sau3AI GATC 4 cut(s) 108, 207, 312, 318
Sau96I GGNCC 1 cut(s) 260
SetI ASST 5 cut(s) 34, 89, 186, 381, 411
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 38
SinI GGWCC 1 cut(s) 260
SspMI CTAG 3 cut(s) 212, 264, 278
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 253, 315, 413
TatI WGTACW 1 cut(s) 191
TauI GCSGC 4 cut(s) 179, 234, 242, 284
Tru1I TTAA 1 cut(s) 359
Tru9I TTAA 1 cut(s) 359
TscAI CASTG 1 cut(s) 61
TseI GCWGC 3 cut(s) 37, 386, 446
TspRI CASTG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 260
XceI RCATGY 2 cut(s) 198, 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XspI CTAG 3 cut(s) 212, 264, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.