Rmu_sc0000007.1_g000018

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000007.1
OG0010617
Physical Location & Seq
Reverse (-)
103594 .. 106261
2668 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
atgaacttggagattgaatctgactctgctattttggttcagttgatgcaaatgcctaatctctcccttcatcctctcagatccctactcaatggatgcaaactgtttatgtttcatatgaccaatacacatctcactcacatattccggaagtataacatggttgctgatgcattagaaaagaatagcatcaaccatgaactgggcatcatcaagtttgatggtcctcctatccatgctgccgaagaatatttagatgatattgctactgtgtctagagccagaagatctggcctgtcgcccacgactccggctctcgccatcgccgcgtcgaaactcatcgtcgtgctctccgcggccagggtcgaggctaagccgacggtcctagtcgctgagaagctcggcgaggccgtcgcccacgactccggctctcgccatcgccgcgtcgaaactcatcgtcccaggcttgacgccaagccgacggtcctcgtcgctgagaagttcggcgaggccgggttggaccttctgaaggaatcgatgaccgccagtagaattgggtacgtcttcgatttgcttctgtctctctcgccggcgactgatcatcacggaaatcgcaacttaatcgtatctccggtcccgggctgcaaccggggtcgttgcaatcggagtcaaatcagtgtgaaggcaaacgttttgacgtcgtccgagacctgcaagcgaagcccagactcgattcctgcttggagcttcggagcttctcagacatctgacaaacaaaactggagaagcccagacccgattccggcgcggagagtttctcggacatctgatgaacaaaaccggcgaagcccagaggggttgggatcgtggagttggatggcgtcatcctctggtggtccgatggtaggacagagtggcagcggtggaggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

310

Amino Acids

33.66

Weight (kDa)

9.25

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 701
Acc36I ACCTGC 1 cut(s) 719
AccB7I CCANNNNNTGG 1 cut(s) 202
AccII CGCG 4 cut(s) 329, 356, 444, 808
AccIII TCCGGA 1 cut(s) 147
AciI CCGC 7 cut(s) 327, 354, 356, 442, 543, 808, 921
AclI AACGTT 1 cut(s) 690
AclWI GGATC 2 cut(s) 75, 871
AcoI YGGCCR 1 cut(s) 357
AcuI CTGAAG 1 cut(s) 548
AcyI GRCGYC 3 cut(s) 471, 698, 881
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 6 cut(s) 202, 360, 529, 638, 802, 905
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 2 cut(s) 359, 461
AluBI AGCT 3 cut(s) 400, 747, 755
AluI AGCT 3 cut(s) 400, 747, 755
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 585, 701
AlwI GGATC 2 cut(s) 75, 871
Ama87I CYCGRG 1 cut(s) 637
Aor13HI TCCGGA 1 cut(s) 147
AoxI GGCC 4 cut(s) 292, 357, 408, 510
ApeKI GCWGC 3 cut(s) 239, 642, 918
AspLEI GCGC 1 cut(s) 808
AspS9I GGNCC 6 cut(s) 224, 382, 484, 520, 634, 896
AsuC2I CCSGG 4 cut(s) 514, 638, 639, 650
AvaI CYCGRG 1 cut(s) 637
AvaII GGWCC 6 cut(s) 224, 382, 484, 520, 634, 896
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 226, 629
BccI CCATC 5 cut(s) 215, 329, 444, 871, 895
BceAI ACGGC 1 cut(s) 395
BcgI CGANNNNNNTGC 2 cut(s) 604, 638
BciT130I CCWGG 2 cut(s) 361, 463
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 4 cut(s) 514, 638, 639, 650
BcoDI GTCTC 2 cut(s) 585, 701
BfaI CTAG 2 cut(s) 276, 386
BfuAI ACCTGC 1 cut(s) 719
BglII AGATCT 1 cut(s) 287
BisI GCNGC 6 cut(s) 240, 327, 357, 442, 643, 919
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 6 cut(s) 241, 328, 358, 443, 644, 920
Bme1390I CCNGG 6 cut(s) 361, 463, 514, 638, 639, 650
Bme18I GGWCC 6 cut(s) 224, 382, 484, 520, 634, 896
BmeT110I CYCGRG 1 cut(s) 637
BmgT120I GGNCC 6 cut(s) 224, 382, 484, 520, 634, 896
BmiI GGNNCC 1 cut(s) 636
BmrFI CCNGG 6 cut(s) 361, 463, 514, 638, 639, 650
BmrI ACTGGG 1 cut(s) 212
BmsI GCATC 5 cut(s) 36, 86, 160, 198, 216
BmuI ACTGGG 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 802, 834
BpmI CTGGAG 1 cut(s) 802
Bpu1102I GCTNAGC 1 cut(s) 372
BpuMI CCSGG 4 cut(s) 514, 638, 639, 650
Bsa29I ATCGAT 1 cut(s) 536
BsaHI GRCGYC 3 cut(s) 471, 698, 881
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 5 cut(s) 354, 360, 461, 637, 649
BsaWI WCCGGW 2 cut(s) 147, 631
Bsc4I CCNNNNNNNGG 6 cut(s) 202, 360, 529, 638, 802, 905
Bse118I RCCGGY 2 cut(s) 589, 840
Bse1I ACTGG 3 cut(s) 207, 546, 785
BseAI TCCGGA 1 cut(s) 147
BseBI CCWGG 2 cut(s) 361, 463
BseCI ATCGAT 1 cut(s) 536
BseDI CCNNGG 5 cut(s) 354, 360, 461, 637, 649
BseGI GGATG 4 cut(s) 70, 101, 882, 884
BseLI CCNNNNNNNGG 6 cut(s) 202, 360, 529, 638, 802, 905
BseMII CTCAG 4 cut(s) 91, 384, 486, 773
BseNI ACTGG 3 cut(s) 207, 546, 785
BseXI GCAGC 2 cut(s) 226, 629
Bsh1236I CGCG 4 cut(s) 329, 356, 444, 808
BshFI GGCC 4 cut(s) 294, 359, 410, 512
BshVI ATCGAT 1 cut(s) 536
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 637
BslFI GGGAC 2 cut(s) 444, 620
BslI CCNNNNNNNGG 6 cut(s) 202, 360, 529, 638, 802, 905
BsmAI GTCTC 2 cut(s) 585, 701
BsmFI GGGAC 2 cut(s) 444, 620
BsnI GGCC 4 cut(s) 294, 359, 410, 512
Bso31I GGTCTC 1 cut(s) 701
BsoBI CYCGRG 1 cut(s) 637
Bsp1286I GDGCHC 1 cut(s) 351
Bsp13I TCCGGA 1 cut(s) 147
Bsp143I GATC 4 cut(s) 80, 287, 598, 863
Bsp1720I GCTNAGC 1 cut(s) 372
BspACI CCGC 7 cut(s) 327, 354, 356, 442, 543, 808, 921
BspANI GGCC 4 cut(s) 294, 359, 410, 512
BspCNI CTCAG 4 cut(s) 90, 385, 487, 772
BspDI ATCGAT 1 cut(s) 536
BspEI TCCGGA 1 cut(s) 147
BspFNI CGCG 4 cut(s) 329, 356, 444, 808
BspLI GGNNCC 1 cut(s) 636
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 2 cut(s) 75, 871
BspTNI GGTCTC 1 cut(s) 701
BsrFI RCCGGY 2 cut(s) 589, 840
BsrI ACTGG 3 cut(s) 207, 546, 785
BssAI RCCGGY 2 cut(s) 589, 840
BssECI CCNNGG 5 cut(s) 354, 360, 461, 637, 649
BssMI GATC 4 cut(s) 80, 287, 598, 863
BssNI GRCGYC 3 cut(s) 471, 698, 881
Bst2UI CCWGG 2 cut(s) 361, 463
Bst4CI ACNGT 4 cut(s) 105, 271, 382, 484
BstACI GRCGYC 3 cut(s) 471, 698, 881
BstC8I GCNNGC 2 cut(s) 591, 716
BstDEI CTNAG 5 cut(s) 77, 372, 393, 495, 759
BstDSI CCRYGG 1 cut(s) 354
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 4 cut(s) 70, 101, 882, 884
BstFNI CGCG 4 cut(s) 329, 356, 444, 808
BstHHI GCGC 1 cut(s) 808
BstKTI GATC 4 cut(s) 83, 290, 601, 866
BstMAI GTCTC 2 cut(s) 585, 701
BstMBI GATC 4 cut(s) 80, 287, 598, 863
BstMWI GCNNNNNNNGC 4 cut(s) 326, 441, 720, 927
BstNI CCWGG 2 cut(s) 361, 463
BstSCI CCNGG 6 cut(s) 359, 461, 512, 636, 637, 648
BstUI CGCG 4 cut(s) 329, 356, 444, 808
BstV1I GCAGC 2 cut(s) 226, 629
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 2 cut(s) 80, 287
BstYI RGATCY 2 cut(s) 80, 287
Bsu15I ATCGAT 1 cut(s) 536
BsuRI GGCC 4 cut(s) 294, 359, 410, 512
BsuTUI ATCGAT 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 354
BtgZI GCGATG 2 cut(s) 307, 422
BtsCI GGATG 4 cut(s) 70, 101, 882, 884
BtsIMutI CAGTG 1 cut(s) 682
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 2 cut(s) 591, 716
CfoI GCGC 1 cut(s) 808
Cfr10I RCCGGY 2 cut(s) 589, 840
Cfr13I GGNCC 6 cut(s) 224, 382, 484, 520, 634, 896
Cfr42I CCGCGG 1 cut(s) 357
Cfr9I CCCGGG 1 cut(s) 637
ClaI ATCGAT 1 cut(s) 536
CseI GACGC 4 cut(s) 318, 433, 479, 870
Csp6I GTAC 1 cut(s) 559
CviAII CATG 3 cut(s) 160, 197, 236
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 5 cut(s) 77, 372, 393, 495, 759
DpnI GATC 4 cut(s) 82, 289, 600, 865
DpnII GATC 4 cut(s) 80, 287, 598, 863
EaeI YGGCCR 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 6 cut(s) 224, 382, 484, 520, 634, 896
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 637
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 359, 461
EcoT22I ATGCAT 1 cut(s) 175
FaeI CATG 3 cut(s) 163, 200, 239
FaiI YATR 8 cut(s) 110, 117, 119, 143, 156, 161, 198, 237
FaqI GGGAC 2 cut(s) 444, 620
FatI CATG 3 cut(s) 159, 196, 235
FauNDI CATATG 1 cut(s) 117
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 6 cut(s) 240, 327, 357, 442, 643, 919
FokI GGATG 4 cut(s) 57, 108, 871, 889
Fsp4HI GCNGC 6 cut(s) 240, 327, 357, 442, 643, 919
FspBI CTAG 2 cut(s) 276, 386
GlaI GCGC 1 cut(s) 807
GluI GCNGC 6 cut(s) 240, 327, 357, 442, 643, 919
GsuI CTGGAG 1 cut(s) 802
HaeIII GGCC 4 cut(s) 294, 359, 410, 512
HgaI GACGC 4 cut(s) 318, 433, 479, 870
HhaI GCGC 1 cut(s) 808
Hin1I GRCGYC 3 cut(s) 471, 698, 881
Hin1II CATG 3 cut(s) 163, 200, 239
Hin6I GCGC 1 cut(s) 806
HinP1I GCGC 1 cut(s) 806
HinfI GANTC 9 cut(s) 17, 23, 307, 422, 533, 667, 728, 733, 799
Hpy188III TCNNGA 2 cut(s) 148, 276
Hpy99I CGWCG 8 cut(s) 334, 347, 382, 416, 449, 484, 494, 703
HpyAV CCTTC 4 cut(s) 77, 523, 533, 676
HpyCH4III ACNGT 4 cut(s) 105, 271, 382, 484
HpyCH4IV ACGT 3 cut(s) 561, 690, 698
HpyCH4V TGCA 6 cut(s) 49, 99, 173, 645, 660, 714
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 441, 720, 927
HpyF3I CTNAG 5 cut(s) 77, 372, 393, 495, 759
HpySE526I ACGT 3 cut(s) 561, 690, 698
Hsp92I GRCGYC 3 cut(s) 471, 698, 881
Hsp92II CATG 3 cut(s) 163, 200, 239
HspAI GCGC 1 cut(s) 806
Kpn2I TCCGGA 1 cut(s) 147
KroI GCCGGC 1 cut(s) 589
KroNI GCCGGC 1 cut(s) 591
Ksp22I TGATCA 1 cut(s) 598
KspI CCGCGG 1 cut(s) 357
Kzo9I GATC 4 cut(s) 80, 287, 598, 863
LmnI GCTCC 2 cut(s) 744, 752
Lsp1109I GCAGC 2 cut(s) 226, 629
LweI GCATC 5 cut(s) 36, 86, 160, 198, 216
MaeI CTAG 2 cut(s) 276, 386
MaeII ACGT 3 cut(s) 561, 690, 698
MalI GATC 4 cut(s) 82, 289, 600, 865
MboI GATC 4 cut(s) 80, 287, 598, 863
MboII GAAGA 3 cut(s) 257, 297, 556
MflI RGATCY 2 cut(s) 80, 287
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 1 cut(s) 552
MlyI GAGTC 5 cut(s) 17, 301, 416, 676, 722
MmeI TCCRAC 2 cut(s) 498, 854
MnlI CCTC 9 cut(s) 84, 237, 361, 400, 497, 502, 847, 898, 920
Mph1103I ATGCAT 1 cut(s) 175
MreI CGCCGGCG 1 cut(s) 589
MroI TCCGGA 1 cut(s) 147
MroNI GCCGGC 1 cut(s) 589
MseI TTAA 1 cut(s) 620
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 2 cut(s) 356, 921
MspR9I CCNGG 6 cut(s) 361, 463, 514, 638, 639, 650
MvaI CCWGG 2 cut(s) 361, 463
MvnI CGCG 4 cut(s) 329, 356, 444, 808
MwoI GCNNNNNNNGC 4 cut(s) 326, 441, 720, 927
NaeI GCCGGC 1 cut(s) 591
NciI CCSGG 4 cut(s) 514, 638, 639, 650
NdeI CATATG 1 cut(s) 117
NdeII GATC 4 cut(s) 80, 287, 598, 863
NgoMIV GCCGGC 1 cut(s) 589
NlaIII CATG 3 cut(s) 163, 200, 239
NlaIV GGNNCC 1 cut(s) 636
NmeAIII GCCGAG 1 cut(s) 381
NsiI ATGCAT 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PdiI GCCGGC 1 cut(s) 591
PfeI GAWTC 4 cut(s) 17, 533, 733, 799
PflFI GACNNNGTC 1 cut(s) 700
PflMI CCANNNNNTGG 1 cut(s) 202
PkrI GCNGC 6 cut(s) 241, 328, 358, 443, 644, 920
PleI GAGTC 5 cut(s) 17, 301, 416, 675, 722
PpsI GAGTC 5 cut(s) 17, 301, 416, 675, 722
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 2 cut(s) 359, 461
PspGI CCWGG 2 cut(s) 359, 461
PspN4I GGNNCC 1 cut(s) 636
PspPI GGNCC 6 cut(s) 224, 382, 484, 520, 634, 896
PsuI RGATCY 2 cut(s) 80, 287
PsyI GACNNNGTC 1 cut(s) 700
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
RseI CAYNNNNRTG 1 cut(s) 344
SacII CCGCGG 1 cut(s) 357
SaqAI TTAA 1 cut(s) 620
SatI GCNGC 6 cut(s) 240, 327, 357, 442, 643, 919
Sau3AI GATC 4 cut(s) 80, 287, 598, 863
Sau96I GGNCC 6 cut(s) 224, 382, 484, 520, 634, 896
SchI GAGTC 5 cut(s) 17, 301, 416, 676, 722
ScrFI CCNGG 6 cut(s) 361, 463, 514, 638, 639, 650
SduI GDGCHC 1 cut(s) 351
SetI ASST 8 cut(s) 402, 525, 564, 693, 701, 713, 749, 757
SfaNI GCATC 5 cut(s) 36, 86, 160, 198, 216
Sfr303I CCGCGG 1 cut(s) 357
SgrAI CRCCGGYG 1 cut(s) 589
SgrBI CCGCGG 1 cut(s) 357
SinI GGWCC 6 cut(s) 224, 382, 484, 520, 634, 896
SmaI CCCGGG 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 1 cut(s) 552
SsiI CCGC 7 cut(s) 327, 354, 356, 442, 543, 808, 921
SspI AATATT 1 cut(s) 251
SspMI CTAG 2 cut(s) 276, 386
StyD4I CCNGG 6 cut(s) 359, 461, 512, 636, 637, 648
TaaI ACNGT 4 cut(s) 105, 271, 382, 484
TaiI ACGT 3 cut(s) 564, 693, 701
TaqI TCGA 6 cut(s) 332, 366, 447, 536, 567, 731
TasI AATT 1 cut(s) 552
TauI GCSGC 3 cut(s) 329, 359, 444
TfiI GAWTC 4 cut(s) 17, 533, 733, 799
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 1 cut(s) 682
TseI GCWGC 3 cut(s) 239, 642, 918
TspDTI ATGAA 5 cut(s) 17, 59, 104, 213, 846
TspGWI ACGGA 1 cut(s) 621
TspMI CCCGGG 1 cut(s) 637
TspRI CASTG 1 cut(s) 682
Tth111I GACNNNGTC 1 cut(s) 700
Van91I CCANNNNNTGG 1 cut(s) 202
VpaK11BI GGWCC 6 cut(s) 224, 382, 484, 520, 634, 896
XagI CCTNNNNNAGG 1 cut(s) 527
XbaI TCTAGA 1 cut(s) 275
XmaI CCCGGG 1 cut(s) 637
XspI CTAG 2 cut(s) 276, 386
ZraI GACGTC 1 cut(s) 699
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.