Rroxscaffold_3G00250020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010617
Physical Location & Seq
Forward (+)
43763398 .. 43768694
5297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250020.1

Sequence Viewer

Length: 525 bp
ATGCACAGAGCAGAGAAATACACAGAGCGAAGAGGGTACGCAGCAAAGAAACAACGAGAAGAAAGTTCCAGGTCTCACTACCGAAGAAAGAAGAAGAAGAACTGCAGGTATACGACTGCATCCATGGTGGTCCGACACGGGTGGTGGTCCGATGCAGCGGTGGGTGAGGCAAGAGGACGCAGACAGAACCTCCTCCTCGCCTATATCGGGTACGCCGATGGAGGTGTTGCGGGCGTGTGGAACGATGGTTGGGACGAAAGGGCAGAGCAGACACTACTAAAGGTGTGGCGAAGACAATCTTCTCTTTCGCAGTCTTGGTGGTCGTTCCCGTTCGGGAGTTGGGTCGGCGGCGGTGGCGAAAAAGTCGGCATAGACAATCATTCTTGGCCTTCTAGTGGGTCCTTCCTATCCGGGATCAGGTCGGAGGCGATGGGGCCGGTTTTGGAGCAGTGGTGGATGGATGGTGTTGACAATAGGGAGGTGATGGTGTTGGTTTTTTTAGTCCTATGTCCGAATGTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.97

Weight (kDa)

9.84

Isoelectric Point (pI)

59.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 110
AciI CCGC 4 cut(s) 158, 230, 348, 351
AclWI GGATC 1 cut(s) 422
AfaI GTAC 2 cut(s) 38, 212
AfiI CCNNNNNNNGG 3 cut(s) 207, 395, 417
AjnI CCWGG 1 cut(s) 68
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 1 cut(s) 422
AoxI GGCC 2 cut(s) 386, 434
ApeKI GCWGC 2 cut(s) 41, 155
AspS9I GGNCC 4 cut(s) 130, 147, 399, 434
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 2 cut(s) 176, 493
AvaII GGWCC 3 cut(s) 130, 147, 399
BbsI GAAGAC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 53, 167
BccI CCATC 6 cut(s) 212, 239, 424, 451, 455, 478
BciT130I CCWGG 1 cut(s) 70
BcnI CCSGG 1 cut(s) 412
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 103
BfuAI ACCTGC 1 cut(s) 96
BisI GCNGC 3 cut(s) 42, 156, 349
BlsI GCNGC 3 cut(s) 43, 157, 350
Bme1390I CCNGG 2 cut(s) 70, 412
Bme18I GGWCC 3 cut(s) 130, 147, 399
BmgT120I GGNCC 4 cut(s) 130, 147, 399, 434
BmiI GGNNCC 2 cut(s) 400, 435
BmrFI CCNGG 2 cut(s) 70, 412
BmsI GCATC 2 cut(s) 128, 142
BpiI GAAGAC 1 cut(s) 298
BpuMI CCSGG 1 cut(s) 412
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 437, 467
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 395, 417
Bse118I RCCGGY 1 cut(s) 436
BseBI CCWGG 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 3 cut(s) 119, 462, 466
BseLI CCNNNNNNNGG 3 cut(s) 207, 395, 417
BseRI GAGGAG 2 cut(s) 182, 185
BseXI GCAGC 2 cut(s) 53, 167
BshFI GGCC 2 cut(s) 388, 436
BsiSI CCGG 3 cut(s) 411, 437, 520
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 3 cut(s) 207, 395, 417
BsmAI GTCTC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 2 cut(s) 388, 436
Bso31I GGTCTC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 414
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 4 cut(s) 158, 230, 348, 351
BspANI GGCC 2 cut(s) 388, 436
BspLI GGNNCC 2 cut(s) 400, 435
BspMAI CTGCAG 1 cut(s) 107
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 436
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 1 cut(s) 414
BssNAI GTATAC 1 cut(s) 111
BssT1I CCWWGG 1 cut(s) 123
Bst1107I GTATAC 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 25
BstC8I GCNNGC 1 cut(s) 232
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 119, 462, 466
BstKTI GATC 1 cut(s) 417
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 1 cut(s) 414
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 2 cut(s) 68, 410
BstSFI CTRYAG 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 53, 167
BstV2I GAAGAC 1 cut(s) 298
BstZ17I GTATAC 1 cut(s) 111
BsuRI GGCC 2 cut(s) 388, 436
BtgI CCRYGG 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 443
BtsCI GGATG 3 cut(s) 119, 462, 466
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 1 cut(s) 455
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 4 cut(s) 130, 147, 399, 434
CseI GACGC 1 cut(s) 186
Csp6I GTAC 2 cut(s) 37, 211
CviAII CATG 1 cut(s) 124
CviJI RGCY 2 cut(s) 388, 436
CviKI_1 RGCY 2 cut(s) 388, 436
CviQI GTAC 2 cut(s) 37, 211
DpnI GATC 1 cut(s) 416
DpnII GATC 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 78
Eco47I GGWCC 3 cut(s) 130, 147, 399
EcoO109I RGGNCCY 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 1 cut(s) 127
FaiI YATR 5 cut(s) 111, 125, 204, 371, 508
FalI AAGNNNNNCTT 2 cut(s) 283, 315
FaqI GGGAC 1 cut(s) 266
FatI CATG 1 cut(s) 123
FauI CCCGC 1 cut(s) 223
FblI GTMKAC 1 cut(s) 110
Fnu4HI GCNGC 3 cut(s) 42, 156, 349
FokI GGATG 3 cut(s) 106, 469, 473
Fsp4HI GCNGC 3 cut(s) 42, 156, 349
FspBI CTAG 1 cut(s) 393
GluI GCNGC 3 cut(s) 42, 156, 349
HaeIII GGCC 2 cut(s) 388, 436
HapII CCGG 3 cut(s) 411, 437, 520
HgaI GACGC 1 cut(s) 186
Hin1II CATG 1 cut(s) 127
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HpaII CCGG 3 cut(s) 411, 437, 520
HphI GGTGA 2 cut(s) 176, 493
Hpy166II GTNNAC 2 cut(s) 111, 469
Hpy188I TCNGA 4 cut(s) 134, 151, 424, 513
Hpy188III TCNNGA 1 cut(s) 334
Hpy8I GTNNAC 2 cut(s) 111, 469
HpyAV CCTTC 2 cut(s) 399, 412
HpyCH4V TGCA 4 cut(s) 4, 105, 119, 155
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
Hsp92II CATG 1 cut(s) 127
Kzo9I GATC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 445
LpnPI CCDG 6 cut(s) 55, 82, 91, 403, 424, 450
Lsp1109I GCAGC 2 cut(s) 53, 167
LweI GCATC 2 cut(s) 128, 142
MaeI CTAG 1 cut(s) 393
MalI GATC 1 cut(s) 416
MboI GATC 1 cut(s) 414
MboII GAAGA 8 cut(s) 42, 71, 96, 103, 106, 109, 291, 303
MmeI TCCRAC 2 cut(s) 157, 402
MnlI CCTC 9 cut(s) 26, 160, 167, 200, 203, 206, 215, 418, 472
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 3 cut(s) 411, 437, 520
MspR9I CCNGG 2 cut(s) 70, 412
MvaI CCWGG 1 cut(s) 70
MwoI GCNNNNNNNGC 1 cut(s) 354
NciI CCSGG 1 cut(s) 412
NcoI CCATGG 1 cut(s) 123
NdeII GATC 1 cut(s) 414
NlaIII CATG 1 cut(s) 127
NlaIV GGNNCC 2 cut(s) 400, 435
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfoI TCCNGGA 1 cut(s) 410
PkrI GCNGC 3 cut(s) 43, 157, 350
PpuMI RGGWCCY 1 cut(s) 399
Psp5II RGGWCCY 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 2 cut(s) 400, 435
PspPI GGNCC 4 cut(s) 130, 147, 399, 434
PspPPI RGGWCCY 1 cut(s) 399
PstI CTGCAG 1 cut(s) 107
RsaI GTAC 2 cut(s) 38, 212
RsaNI GTAC 2 cut(s) 37, 211
SatI GCNGC 3 cut(s) 42, 156, 349
Sau3AI GATC 1 cut(s) 414
Sau96I GGNCC 4 cut(s) 130, 147, 399, 434
ScrFI CCNGG 2 cut(s) 70, 412
SetI ASST 7 cut(s) 74, 110, 192, 226, 285, 422, 483
SfaNI GCATC 2 cut(s) 128, 142
SfcI CTRYAG 1 cut(s) 103
SinI GGWCC 3 cut(s) 130, 147, 399
SsiI CCGC 4 cut(s) 158, 230, 348, 351
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 2 cut(s) 68, 410
StyI CCWWGG 1 cut(s) 123
TauI GCSGC 1 cut(s) 351
TscAI CASTG 1 cut(s) 455
TseI GCWGC 2 cut(s) 41, 155
TspRI CASTG 1 cut(s) 455
VpaK11BI GGWCC 3 cut(s) 130, 147, 399
XmiI GTMKAC 1 cut(s) 110
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.