Rmu_sc0000363.1_g000055

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000363.1
OG0002998
Physical Location & Seq
Forward (+)
228956 .. 230465
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
atggcagtgtcggatgacgaatctgacgagctcggccggatgcgcgttcgagttcgccggaagcgcaagaaactccaccaccgattcaagaacggagtggtgaggaatctggtcagctactggcccctgttctgggcccttgtaatttttctagcggccatttgtttgcttctgtatgaggcatggactatcggctggaaatccccacaactcaataactcagaactcactgcggtgaacaatgatttgaatcggcttgatcctatcactcaggttgttcgtggcgttacgcaacgttgcttgccaatcttacctcctgaacaacttgagcatctggaaattcctgtgccccaagattccactagtcctgttaaaaaattgatatacacagctgagaatgatgctccgttgttaggacggtacaacaccctatctcaagagcacacagatgcaacaagatttaatttgtttactggaaatcaaactctagatcagagagatgaaatgtttaaggtaaatgaaactgttactataaactgtggtttctacagtgaaagtggtgggtttaagatctcaaatgaagacaagaattacatgaaaagttgtgacattgtagtctccacttgtgcatttggtagtggagatgatctttatcaacccattggaatgtcagaggcatcccttcagaaggtttgctttgttgctttttgggatgaagttactgtgagtactcaagagtcagctgaacatagaattggtgaggatggatttattgggaaatggcgcattgtggttgtaagagatctcccttttgcggaccaaaggttaaatggaaaaatcccaaagtcgcatcaatttcagaagtatgttaagcagatgacggctgacaatattccatataatatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

302

Amino Acids

34.54

Weight (kDa)

6.33

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 147 - 283 8.4e-47 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AccII CGCG 1 cut(s) 45
AciI CCGC 3 cut(s) 155, 233, 815
AclI AACGTT 1 cut(s) 295
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 2 cut(s) 34, 156
AcsI RAATTY 1 cut(s) 339
AcuI CTGAAG 1 cut(s) 668
AfaI GTAC 2 cut(s) 422, 730
AfiI CCNNNNNNNGG 5 cut(s) 132, 133, 413, 688, 814
AgsI TTSAA 2 cut(s) 88, 250
AhlI ACTAGT 1 cut(s) 362
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
AleI CACNNNNGTG 1 cut(s) 233
AluBI AGCT 4 cut(s) 31, 117, 392, 743
AluI AGCT 4 cut(s) 31, 117, 392, 743
Alw21I GWGCWC 2 cut(s) 33, 444
Alw26I GTCTC 1 cut(s) 622
AlwI GGATC 1 cut(s) 254
AlwNI CAGNNNCTG 1 cut(s) 120
AoxI GGCC 4 cut(s) 34, 122, 135, 156
ApaI GGGCCC 1 cut(s) 139
ApoI RAATTY 1 cut(s) 339
AspLEI GCGC 3 cut(s) 45, 66, 786
AspS9I GGNCC 4 cut(s) 123, 135, 136, 817
AsuHPI GGTGA 3 cut(s) 112, 247, 770
AvaII GGWCC 1 cut(s) 817
BaeGI GKGCMC 2 cut(s) 139, 351
BanII GRGCYC 2 cut(s) 33, 139
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 2 cut(s) 33, 444
BccI CCATC 1 cut(s) 758
BceAI ACGGC 1 cut(s) 897
BcoDI GTCTC 1 cut(s) 622
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 3 cut(s) 152, 363, 488
BfmI CTRYAG 1 cut(s) 547
BglII AGATCT 2 cut(s) 570, 802
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
BmcAI AGTACT 1 cut(s) 730
Bme18I GGWCC 1 cut(s) 817
BmgT120I GGNCC 4 cut(s) 123, 135, 136, 817
BmiI GGNNCC 2 cut(s) 125, 137
BmsI GCATC 6 cut(s) 30, 340, 391, 439, 686, 859
BpiI GAAGAC 1 cut(s) 588
BpuEI CTTGAG 3 cut(s) 347, 420, 717
BsaBI GATNNNNATC 2 cut(s) 249, 651
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 133, 413, 688, 814
Bse1I ACTGG 2 cut(s) 125, 478
Bse8I GATNNNNATC 2 cut(s) 249, 651
BseGI GGATG 5 cut(s) 19, 45, 677, 718, 769
BseJI GATNNNNATC 2 cut(s) 249, 651
BseLI CCNNNNNNNGG 5 cut(s) 132, 133, 413, 688, 814
BseMII CTCAG 3 cut(s) 234, 284, 384
BseNI ACTGG 2 cut(s) 125, 478
BseSI GKGCMC 2 cut(s) 139, 351
BseX3I CGGCCG 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 4 cut(s) 36, 124, 137, 158
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 33, 444
BsiSI CCGG 2 cut(s) 37, 58
BslI CCNNNNNNNGG 5 cut(s) 132, 133, 413, 688, 814
BsmAI GTCTC 1 cut(s) 622
BsnI GGCC 4 cut(s) 36, 124, 137, 158
Bsp120I GGGCCC 1 cut(s) 135
Bsp1286I GDGCHC 4 cut(s) 33, 139, 351, 444
Bsp143I GATC 5 cut(s) 259, 490, 570, 646, 802
BspACI CCGC 3 cut(s) 155, 233, 815
BspANI GGCC 4 cut(s) 36, 124, 137, 158
BspCNI CTCAG 3 cut(s) 233, 283, 385
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 2 cut(s) 125, 137
BspPI GGATC 1 cut(s) 254
BsrI ACTGG 2 cut(s) 125, 478
BssMI GATC 5 cut(s) 259, 490, 570, 646, 802
Bst4CI ACNGT 5 cut(s) 420, 526, 539, 551, 724
BstC8I GCNNGC 1 cut(s) 302
BstDEI CTNAG 3 cut(s) 220, 270, 393
BstENI CCTNNNNNAGG 1 cut(s) 686
BstF5I GGATG 5 cut(s) 19, 45, 677, 718, 769
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 3 cut(s) 45, 66, 786
BstKTI GATC 5 cut(s) 262, 493, 573, 649, 805
BstMAI GTCTC 1 cut(s) 622
BstMBI GATC 5 cut(s) 259, 490, 570, 646, 802
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 2 cut(s) 42, 63
BstSFI CTRYAG 1 cut(s) 547
BstSLI GKGCMC 2 cut(s) 139, 351
BstUI CGCG 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 2 cut(s) 570, 802
BstYI RGATCY 2 cut(s) 570, 802
BstZI CGGCCG 1 cut(s) 34
BsuRI GGCC 4 cut(s) 36, 124, 137, 158
BtsCI GGATG 5 cut(s) 19, 45, 677, 718, 769
BtsI GCAGTG 2 cut(s) 12, 228
BtsIMutI CAGTG 3 cut(s) 12, 228, 556
Cac8I GCNNGC 1 cut(s) 302
CaiI CAGNNNCTG 1 cut(s) 120
CfoI GCGC 3 cut(s) 45, 66, 786
Cfr13I GGNCC 4 cut(s) 123, 135, 136, 817
Csp6I GTAC 2 cut(s) 421, 729
CviAII CATG 2 cut(s) 183, 595
CviQI GTAC 2 cut(s) 421, 729
DdeI CTNAG 3 cut(s) 220, 270, 393
DpnI GATC 5 cut(s) 261, 492, 572, 648, 804
DpnII GATC 5 cut(s) 259, 490, 570, 646, 802
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
EaeI YGGCCR 2 cut(s) 34, 156
EagI CGGCCG 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 31
EclXI CGGCCG 1 cut(s) 34
Eco24I GRGCYC 2 cut(s) 33, 139
Eco47I GGWCC 1 cut(s) 817
Eco52I CGGCCG 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 31
EcoNI CCTNNNNNAGG 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 136
EcoT38I GRGCYC 2 cut(s) 33, 139
FaeI CATG 2 cut(s) 186, 598
FaiI YATR 9 cut(s) 177, 184, 385, 533, 596, 750, 867, 898, 900
FalI AAGNNNNNCTT 2 cut(s) 680, 712
FatI CATG 2 cut(s) 182, 594
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 5 cut(s) 26, 52, 664, 725, 776
FriOI GRGCYC 2 cut(s) 33, 139
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 3 cut(s) 152, 363, 488
GlaI GCGC 3 cut(s) 44, 65, 785
GluI GCNGC 1 cut(s) 156
HaeIII GGCC 4 cut(s) 36, 124, 137, 158
HapII CCGG 2 cut(s) 37, 58
HhaI GCGC 3 cut(s) 45, 66, 786
Hin1II CATG 2 cut(s) 186, 598
Hin6I GCGC 3 cut(s) 43, 64, 784
HinP1I GCGC 3 cut(s) 43, 64, 784
HinfI GANTC 6 cut(s) 20, 84, 106, 250, 356, 737
HpaII CCGG 2 cut(s) 37, 58
HphI GGTGA 3 cut(s) 112, 247, 770
Hpy166II GTNNAC 2 cut(s) 238, 471
Hpy188I TCNGA 8 cut(s) 13, 25, 223, 495, 673, 687, 861, 908
Hpy188III TCNNGA 6 cut(s) 88, 317, 335, 437, 488, 734
Hpy8I GTNNAC 2 cut(s) 238, 471
HpyAV CCTTC 2 cut(s) 682, 692
HpyCH4III ACNGT 5 cut(s) 420, 526, 539, 551, 724
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 2 cut(s) 452, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 63
HpyF3I CTNAG 3 cut(s) 220, 270, 393
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 2 cut(s) 186, 598
HspAI GCGC 3 cut(s) 43, 64, 784
Kzo9I GATC 5 cut(s) 259, 490, 570, 646, 802
LmnI GCTCC 1 cut(s) 409
LweI GCATC 6 cut(s) 30, 340, 391, 439, 686, 859
MaeI CTAG 3 cut(s) 152, 363, 488
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 4 cut(s) 286, 526, 605, 718
MalI GATC 5 cut(s) 261, 492, 572, 648, 804
MboI GATC 5 cut(s) 259, 490, 570, 646, 802
MboII GAAGA 1 cut(s) 593
MflI RGATCY 2 cut(s) 570, 802
MhlI GDGCHC 4 cut(s) 33, 139, 351, 444
MluCI AATT 7 cut(s) 144, 339, 377, 463, 589, 753, 854
MlyI GAGTC 1 cut(s) 746
MnlI CCTC 5 cut(s) 96, 172, 324, 667, 754
MseI TTAA 6 cut(s) 372, 462, 510, 567, 827, 870
MslI CAYNNNNRTG 3 cut(s) 233, 447, 665
MspA1I CMGCKG 2 cut(s) 392, 743
MspI CCGG 2 cut(s) 37, 58
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 42, 63
NdeII GATC 5 cut(s) 259, 490, 570, 646, 802
NlaIII CATG 2 cut(s) 186, 598
NlaIV GGNNCC 2 cut(s) 125, 137
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 1 cut(s) 605
OliI CACNNNNGTG 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 5 cut(s) 20, 84, 106, 250, 356
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 1 cut(s) 745
PpsI GAGTC 1 cut(s) 745
Psp124BI GAGCTC 1 cut(s) 33
Psp1406I AACGTT 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 125, 137
PspOMI GGGCCC 1 cut(s) 135
PspPI GGNCC 4 cut(s) 123, 135, 136, 817
PstNI CAGNNNCTG 1 cut(s) 120
PsuI RGATCY 2 cut(s) 570, 802
PvuII CAGCTG 2 cut(s) 392, 743
RsaI GTAC 2 cut(s) 422, 730
RsaNI GTAC 2 cut(s) 421, 729
RseI CAYNNNNRTG 3 cut(s) 233, 447, 665
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 6 cut(s) 372, 462, 510, 567, 827, 870
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 5 cut(s) 259, 490, 570, 646, 802
Sau96I GGNCC 4 cut(s) 123, 135, 136, 817
ScaI AGTACT 1 cut(s) 730
SchI GAGTC 1 cut(s) 746
SduI GDGCHC 4 cut(s) 33, 139, 351, 444
SfaNI GCATC 6 cut(s) 30, 340, 391, 439, 686, 859
SfcI CTRYAG 1 cut(s) 547
SinI GGWCC 1 cut(s) 817
SmiMI CAYNNNNRTG 3 cut(s) 233, 447, 665
SmlI CTYRAG 3 cut(s) 326, 435, 732
SmoI CTYRAG 3 cut(s) 326, 435, 732
SpeI ACTAGT 1 cut(s) 362
Sse9I AATT 7 cut(s) 144, 339, 377, 463, 589, 753, 854
SsiI CCGC 3 cut(s) 155, 233, 815
SspI AATATT 1 cut(s) 892
SspMI CTAG 3 cut(s) 152, 363, 488
SstI GAGCTC 1 cut(s) 33
TaaI ACNGT 5 cut(s) 420, 526, 539, 551, 724
TaiI ACGT 1 cut(s) 298
TaqI TCGA 1 cut(s) 49
TasI AATT 7 cut(s) 144, 339, 377, 463, 589, 753, 854
TatI WGTACW 1 cut(s) 728
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 5 cut(s) 20, 84, 106, 250, 356
Tru1I TTAA 6 cut(s) 372, 462, 510, 567, 827, 870
Tru9I TTAA 6 cut(s) 372, 462, 510, 567, 827, 870
TscAI CASTG 3 cut(s) 12, 235, 556
TseFI GTSAC 1 cut(s) 605
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 5 cut(s) 516, 534, 594, 611, 729
TspGWI ACGGA 2 cut(s) 108, 396
TspRI CASTG 3 cut(s) 12, 235, 556
VpaK11BI GGWCC 1 cut(s) 817
XagI CCTNNNNNAGG 1 cut(s) 686
XapI RAATTY 1 cut(s) 339
XbaI TCTAGA 1 cut(s) 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XspI CTAG 3 cut(s) 152, 363, 488
ZrmI AGTACT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.