Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000540.1

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Reverse (-)

115225 .. 129040

Gene Structure 13816 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5847 bp

atgatagcggaaggataccaatttgaagcagttcgatcaaggttgaagctcctgcttcatttttcttcaccaagcatgtcggaagctcaaaggagattggtgacaactattagtcttcctcgtaataatggttacgtaaatgggggactatcaatcaggtcccctgctgcagcacctgcagttgaggagttttgctatgcccttggtgggaagaagccaatccatagcattttaatagcaaataatggaatggcagcagtcaagtttatacgtagtgtgaggacatgggcttatgaaacatttggtacagaaaaggctgttttgttggtggccatggccacaccagaagacatgagaatcaatgcagaacacattagaattgctgatcagtttgtggaagttcctggtggaactaacaataataactatgccaatgtgcaactcattgtagagatggcagagataacacatgttgatgcagtttggcctggttggggccatgcatctgagatccctgagcttccagatgcattgactgcaaagggcatcatatttctcgggccaccagctgtatctatggcagcattaggagataaaattggttcctcattaattgctcaagctgctgaagtacccacccttccatggagtggatcgcatgtgaaaattgcttcagacagctgcctggttacgatcgcagatgaaatatatcgggaagcatgtgtttacacaacagaggaagcgattgcaagttgtcaggttgttggttacccagccatgattaaggcatcatggggtggtggtggtaaaggcataagaaaggtccataatgatgatgaagttagggcattgttcaagcaagtccagggtgaagttcctggatctcccatattcataatgaaagtcgcatcccagagccgacatttagaagtccagttactatgtgatcagcatggaaatgttgcagctttgcacagccgtgattgcagtgttcaaaggaggcaccagaagattattgaggagggtccaataactgtagctccaccagagacaattaaaaagttggagcaggcggctcgaaggttggctaaaagtgttaactatgttggagcagctactgttgagtatctgtacagtatggacactggcgagtactatttcttagagttgaaccctcggttgcaggtggagcacccagtcactgagtggatagcagaaataaatctgccagcagcccaagttgcagttgggatgggcattcccctctggcaaattcctgagataaggcggttctatggaatggaacatggtagcggatatgatgcttggagaaagacgtcagttgttgccaccccatttgactttgacaaggcagaatctacaaggccaaaaggtcattgtgttgctgtgcgtgtaacaagtgaggatccagatgatggctttaaacctactagtgggaaagttcaggagctgagttttaaaagcaagccaaatgtttgggcgtacttttctgttaagtctggaggggggattcatgagtactcagattctcagtttggacatgtttttgcatttggagagtctcgagctctagctattgcaaatatggttcttgggctgaaggaaattcaaattcgaggagaaattcgtacaaatgtggattattcaatagatcttttacatgcttcagattacagagaaaataaaatccacacaggttggttggatagtagaattgctatgcgggttagaacagaaaggcctccttggtatttgtctgtaattggagggactctttttaaagcatctgctagtagtgcggcgatggtttcagattatattggctatcttgagaaagggcaaatcccacctaagcatatatcatttgtccattcacaagtgtctttgaacattgaggggagcaaatacacgattgacttggtgagagggggtcctgggaattatagattgcggatgaatgagtcagaggttgaagcagagatacatactttacgtgatggaggattactgatgcagttggatggaaacagtcacattatatatgcagaggaagaagcagctggaactcgtcttcttattgatggaaggacttgcttgcttcagaacgatcatgatccatccaagttaatagcagagacaccatgcaagctgttgaggttcttggttgctgatgatagtcatctcgatgctgatacaccatatgcagaggttgaggttatgaagatgtgtatgcctctactttcacctgcttctggagttatccactttcaattgtctgaaggccaagcaatgcaggctggtgaacttattgcaaggcttgatttagatgatccttcagctgtgagaaagacagaacctttccatggaaacttccccgtcctggggcctccaaccgcaatttctggtaaagttcatcaaagatgtgctgcaagcttaaacgcagcccgaatgattcttgctggttatgagcataacattgatgaagtagtgcaaaacttgctcaattgcctagatagtcctgaactccctttccttcaatggcaagaatgcttggctgttttggcaacccgcctacccaagaatctcaaaaatgaactggaatcaaaatttaaggattttgagttgatttctagctctcagaatgttgatttccctgccaaattgttgcgtggtgttcttgaggcccatctatcctcctgtcctgataaagaaaaaggagcccaagaaaggcttgttgaacctttgatgagccttgtgacgtcttatgaaggtggaagagaaagtcatgcccgagttattgttcaatccctctttgaagagtatctgtcagttgaagaattattcagtgacaacattcaggctgatgtgattgagcgccttcgacttcaatataagaaggatctgttgaaggttgtggaaattgtgctctctcatcaggctgatgtgattgagcgccttcgacttcaatataagaaggatctgttgaaggttgtggaaattgtgctctctcatcagggtgtaaagaataaaaataagttgattccacgactcatggaacaactggtgtaccccaaccctgctgcatacagggagaagctaattcggttttctcaacttaaccatacaaactattctcagttggcactaaaagcaagtcagcttatggagcaaaccaagttgagtgaacttcgttctagcattgctagaagtctttctgagttggagatgtttactgaggatggtgaaaccatggatactccaaagaggaaaagtgccataaatgaacgaatggaggatcttgtgagtgcacctttggcagttgaagatgcccttgtaggtctctttgatcatggtgatcacacccttcagaggcgggttgttgagagttatgtacgccggttataccagccctaccttgtaaaggggagtgtcaggatgcagtggcacagatctggtctaatagcttcatgggagttctcggaagaacctgttgagaggaaacatgagattgaaggtcaatcagttgataaaacattcctccagaaacacgaggagaggaaatggggagtcatggttataataaaatctcttcagtttcttccagcagttattagtggtgcactgaaggaaatgtctcataaccttcatgaagcaactccaaatggatctactgaaccaagtggctttggtaatatgatgcatattgctttggttggcatcaacaatcagatgagtctacttcaggatagtggtgatgaggatcaggctcaagaaagaattaagaagttagccaaaatacttaaagagcaaggagtagcctccagtctacacagtgcaggtgtcacagtaattagttgtatcatccaaagggatgaagggcgggcccctatgaggcactccttccactggtcatcagagaagctctattatgaagaagaacccctattgcgtcacctagagcctcctctatctatttaccttgaattggacaagcttaaaggctatgagaatatacagtataccccatctcgtgaccgtcaatggcacttgtacaccgttgtagacaagccactaccaatccaaaggatgttcctcagaacacttgtcaggcagcccacaacaaatgaagggagcatcttgaggtccttatcaacagcaatggaagagttggaactgaatgcgcacaatgccactgtcaaatctgatcacacccatatgtatctttatatcttacgtgagcaacaaattaatgatctgctgccttaccacaagagagttgatttagatgctcaacaagaagaaactgttgtggaggccatcttggacgaacttgcacgtgaaatccacacatcagttggtgtaaggatgcataggttgggtgtttgtgagtgggaagtgaagttgtggatggcatcctctggagaggcaaacattgcttggagggttgtcgtgacaaatgtgactgctgatgatcttgaaggaatatctgctattctaaagtggcttagctatgttcctccccatgttggtggcccactccccatttcaattcccttggatcctccagaaaggcgtgtggagtaccgcccggagaattcatgtgatcctcgtgctgcgatttctggtgctctgaatggtaatgggaactggatggggggtatattcgacaaagacagctttgttgagacgctagaaggctgggcaaggaccgttgttactggaagggcgaagcttggaggaatccctgtgggaattgttgctgttgagacacagacggtgacgcaaattattcctgctgatccaggccagcttgattctcatgagagggttgttcctcaggctggacaagtatggtttcctgattctgcaactaagactgcccaagcattattagatttcaacagagaaggactcccacttttcattcttgccaactggagaggcttttctggtgggcagagagaccttttcgaagggatccttcaggctggatcaaccattgtggagaacctaaggacttacaaacagcccgtttttgtattcatcccaatgatgggtgagctccgcggtggagcatgggtggtcgttgatagccggataaatccggaccatattgaaatgtatgctgatcgaacggctagaggaaatgtgcttgagccagaggggatgatcgagatcaagtttaggaacaaggaattgctggagtgcatgggcaggcttgaccaacagctgatccaactaaagactaagcttcaggaagccaggagctgtggggcgaatgagaaggttgaatccctacagtttcagatacgatcccgagaaaaacagcttttgcccgtctacactcagatagctaccaaatttgcagaacttcatgatacgtctctgaggatggctgcaaaaggggtaatcagaggagttttggagtgggccagctctcgatctttcttctacaagagattgcgtcggagaattgccgatgactcgctcatcaagatagtgagagatgctgctggtgaacagctgtcacatagatctgcaatcgatttgatcaagagctggtttttgagttctgatatttcaagaggcagggaaaatgcttgggaggatgatgaaaccttctttagatggaaggacgatcaaactaattatgagggtaagctaaaagaattgcgggtccagaaggtattgcttcaattggcaaccattggcaactcagcatctgatttgcaagccctacctcaaggtcttgctgcccttctaagcaaggttgagccatcaagcagatcatttttggttgaggaactccggaaggtgcttggttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

1948

Amino Acids

218.11

Weight (kDa)

5.98

Isoelectric Point (pI)

44.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	74 - 193	4.2e-28	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	241 - 423	3.8e-46	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	242 - 393	2.3e-07	ATP-grasp domain
ATP-grasp_3	PF02655	249 - 395	2.3e-07	ATP-grasp domain
Biotin_carb_C	PF02785	470 - 576	9.4e-24	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	592 - 707	9.5e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	718 - 780	7e-08	Biotin-requiring enzyme
ACC_central	PF08326	781 - 986	6.8e-64	Acetyl-CoA carboxylase, central region
ACC_central	PF08326	986 - 1495	6.3e-105	Acetyl-CoA carboxylase, central region
Carboxyl_trans	PF01039	1493 - 1832	1.3e-85	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3599
AarI	CACCTGC	4 cut(s)	184, 1174, 2275, 3848
AatII	GACGTC	2 cut(s)	1340, 2783
Acc16I	TGCGCA	1 cut(s)	4203
Acc36I	ACCTGC	4 cut(s)	184, 1174, 2275, 3848
AccB1I	GGYRCC	1 cut(s)	1002
AccB7I	CCANNNNNTGG	2 cut(s)	650, 1436
AccI	GTMKAC	5 cut(s)	3757, 3847, 4040, 4083, 5361
AccII	CGCG	1 cut(s)	5088
AccIII	TCCGGA	2 cut(s)	5125, 5829
AcoI	YGGCCR	2 cut(s)	330, 336
AcsI	RAATTY	7 cut(s)	1272, 1626, 1632, 1644, 2627, 4615, 5381
AcvI	CACGTG	1 cut(s)	4358
AcyI	GRCGYC	2 cut(s)	1337, 2780
AdeI	CACNNNGTG	1 cut(s)	1206
AflIII	ACRYGT	2 cut(s)	469, 1561
AhlI	ACTAGT	1 cut(s)	1451
AjnI	CCWGG	9 cut(s)	403, 487, 684, 864, 877, 1954, 2400, 4822, 5282
AjuI	GAANNNNNNNTTGG	2 cut(s)	2404, 2436
AleI	CACNNNNGTG	1 cut(s)	978
AloI	GAACNNNNNNTCC	2 cut(s)	2535, 2567
Alw21I	GWGCWC	8 cut(s)	1194, 1591, 2949, 3027, 3331, 3643, 4651, 5085
Alw26I	GTCTC	9 cut(s)	1043, 1587, 2150, 3365, 3660, 4700, 4781, 4977, 5409
Alw44I	GTGCAC	2 cut(s)	3327, 3639
AlwNI	CAGNNNCTG	6 cut(s)	176, 1118, 1202, 1471, 5289, 5744
Ama87I	CYCGRG	4 cut(s)	557, 1584, 2811, 5337
Aor13HI	TCCGGA	2 cut(s)	5125, 5829
ApaI	GGGCCC	1 cut(s)	3907
ApaLI	GTGCAC	2 cut(s)	3327, 3639
ApoI	RAATTY	7 cut(s)	1272, 1626, 1632, 1644, 2627, 4615, 5381
AseI	ATTAAT	2 cut(s)	611, 4269
Asp700I	GAANNNNTTC	2 cut(s)	30, 3232
AspLEI	GCGC	3 cut(s)	2898, 2976, 4204
AsuC2I	CCSGG	1 cut(s)	4610
AsuII	TTCGAA	1 cut(s)	4992
AvaI	CYCGRG	4 cut(s)	557, 1584, 2811, 5337
AvaII	GGWCC	8 cut(s)	159, 823, 1025, 1952, 4164, 4728, 5128, 5698
AxyI	CCTNAGG	2 cut(s)	4857, 5033
BaeGI	GKGCMC	3 cut(s)	3331, 3643, 3907
BaeI	ACNNNNGTAYC	2 cut(s)	3071, 3104
BalI	TGGCCA	2 cut(s)	332, 338
BamHI	GGATCC	3 cut(s)	1426, 4579, 4998
BanI	GGYRCC	1 cut(s)	1002
BanII	GRGCYC	4 cut(s)	1591, 2743, 3907, 5085
BarI	GAAGNNNNNNTAC	6 cut(s)	624, 656, 921, 953, 1986, 2018
BauI	CACGAG	3 cut(s)	3569, 4050, 4629
BbrPI	CACGTG	1 cut(s)	4358
BbsI	GAAGAC	3 cut(s)	107, 354, 2084
Bbv12I	GWGCWC	8 cut(s)	1194, 1591, 2949, 3027, 3331, 3643, 4651, 5085
BceAI	ACGGC	2 cut(s)	963, 5172
BcgI	CGANNNNNNTGC	2 cut(s)	1058, 1092
BciT130I	CCWGG	9 cut(s)	405, 489, 686, 866, 879, 1956, 2402, 4824, 5284
BciVI	GTATCC	2 cut(s)	8, 3268
BclI	TGATCA	6 cut(s)	385, 946, 3367, 3376, 4225, 5571
BcnI	CCSGG	1 cut(s)	4610
BcoDI	GTCTC	9 cut(s)	1043, 1587, 2150, 3365, 3660, 4700, 4781, 4977, 5409
BcuI	ACTAGT	1 cut(s)	1451
BfmI	CTRYAG	4 cut(s)	168, 177, 1035, 5318
BfoI	RGCGCY	2 cut(s)	2899, 2977
BfuAI	ACCTGC	4 cut(s)	184, 1174, 2275, 3848
BfuI	GTATCC	2 cut(s)	8, 3268
BglII	AGATCT	3 cut(s)	1672, 3470, 5555
BlpI	GCTNAGC	1 cut(s)	4526
BmcAI	AGTACT	2 cut(s)	1154, 1541
Bme18I	GGWCC	8 cut(s)	159, 823, 1025, 1952, 4164, 4728, 5128, 5698
BmeT110I	CYCGRG	4 cut(s)	557, 1584, 2811, 5337
BmrI	ACTGGG	1 cut(s)	1190
BmuI	ACTGGG	1 cut(s)	1190
BpiI	GAAGAC	3 cut(s)	107, 354, 2084
BplI	GAGNNNNNCTC	2 cut(s)	4917, 4949
BpmI	CTGGAG	8 cut(s)	1542, 2295, 3545, 3826, 4461, 4569, 4978, 5243
Bpu10I	CCTNAGC	2 cut(s)	516, 1872
Bpu1102I	GCTNAGC	1 cut(s)	4526
Bpu14I	TTCGAA	1 cut(s)	4992
BpuEI	CTTGAG	7 cut(s)	603, 1871, 2720, 3774, 4180, 5195, 5748
BpuMI	CCSGG	1 cut(s)	4610
Bsa29I	ATCGAT	1 cut(s)	5565
BsaAI	YACGTR	5 cut(s)	136, 272, 2015, 4256, 4358
BsaBI	GATNNNNATC	4 cut(s)	2133, 2199, 3780, 5195
BsaHI	GRCGYC	2 cut(s)	1337, 2780
BsaI	GGTCTC	2 cut(s)	3365, 4977
BsaWI	WCCGGW	2 cut(s)	5125, 5829
BsaXI	ACNNNNNCTCC	6 cut(s)	1024, 1054, 3579, 3609, 5085, 5115
Bse118I	RCCGGY	1 cut(s)	3417
Bse21I	CCTNAGG	2 cut(s)	4857, 5033
Bse3DI	GCAATG	4 cut(s)	2316, 3219, 4185, 4452
Bse8I	GATNNNNATC	4 cut(s)	2133, 2199, 3780, 5195
BseAI	TCCGGA	2 cut(s)	5125, 5829
BseBI	CCWGG	9 cut(s)	405, 489, 686, 866, 879, 1956, 2402, 4824, 5284
BseCI	ATCGAT	1 cut(s)	5565
BseJI	GATNNNNATC	4 cut(s)	2133, 2199, 3780, 5195
BseMI	GCAATG	4 cut(s)	2316, 3219, 4185, 4452
BseRI	GAGGAG	6 cut(s)	200, 1034, 1653, 3587, 3975, 5451
BseSI	GKGCMC	3 cut(s)	3331, 3643, 3907
BseYI	CCCAGC	2 cut(s)	772, 4719
BsgI	GTGCAG	1 cut(s)	3876
Bsh1236I	CGCG	1 cut(s)	5088
Bsh1285I	CGRYCG	1 cut(s)	696
BshNI	GGYRCC	1 cut(s)	1002
BshVI	ATCGAT	1 cut(s)	5565
BsiEI	CGRYCG	1 cut(s)	696
BsiHKAI	GWGCWC	8 cut(s)	1194, 1591, 2949, 3027, 3331, 3643, 4651, 5085
BsiHKCI	CYCGRG	4 cut(s)	557, 1584, 2811, 5337
BsiSI	CCGG	5 cut(s)	3418, 4610, 5116, 5126, 5830
BslFI	GGGAC	3 cut(s)	145, 159, 1804
BsmAI	GTCTC	9 cut(s)	1043, 1587, 2150, 3365, 3660, 4700, 4781, 4977, 5409
BsmBI	CGTCTC	2 cut(s)	4700, 5409
BsmFI	GGGAC	3 cut(s)	145, 159, 1804
BsmI	GAATGC	3 cut(s)	1257, 2573, 4204
Bso31I	GGTCTC	2 cut(s)	3365, 4977
BsoBI	CYCGRG	4 cut(s)	557, 1584, 2811, 5337
Bsp119I	TTCGAA	1 cut(s)	4992
Bsp120I	GGGCCC	1 cut(s)	3903
Bsp13I	TCCGGA	2 cut(s)	5125, 5829
Bsp1407I	TGTACA	2 cut(s)	1131, 4071
Bsp1720I	GCTNAGC	1 cut(s)	4526
Bsp19I	CCATGG	4 cut(s)	333, 644, 2383, 3270
BspDI	ATCGAT	1 cut(s)	5565
BspEI	TCCGGA	2 cut(s)	5125, 5829
BspFNI	CGCG	1 cut(s)	5088
BspHI	TCATGA	5 cut(s)	1534, 2131, 3667, 4840, 5395
BspMAI	CTGCAG	2 cut(s)	172, 181
BspMI	ACCTGC	4 cut(s)	184, 1174, 2275, 3848
BspT104I	TTCGAA	1 cut(s)	4992
BspT107I	GGYRCC	1 cut(s)	1002
BspTNI	GGTCTC	2 cut(s)	3365, 4977
BsrDI	GCAATG	4 cut(s)	2316, 3219, 4185, 4452
BsrFI	RCCGGY	1 cut(s)	3417
BsrGI	TGTACA	2 cut(s)	1131, 4071
BssAI	RCCGGY	1 cut(s)	3417
BssNAI	GTATAC	1 cut(s)	4041
BssNI	GRCGYC	2 cut(s)	1337, 2780
BssSI	CACGAG	3 cut(s)	3569, 4050, 4629
BssT1I	CCWWGG	7 cut(s)	202, 333, 644, 1766, 2383, 3270, 4575
Bst1107I	GTATAC	1 cut(s)	4041
Bst2BI	CACGAG	3 cut(s)	3569, 4050, 4629
Bst2UI	CCWGG	9 cut(s)	405, 489, 686, 866, 879, 1956, 2402, 4824, 5284
Bst6I	CTCTTC	4 cut(s)	2791, 2832, 3615, 4179
BstACI	GRCGYC	2 cut(s)	1337, 2780
BstAPI	GCANNNNNTGC	4 cut(s)	176, 536, 2518, 4454
BstAUI	TGTACA	2 cut(s)	1131, 4071
BstBAI	YACGTR	5 cut(s)	136, 272, 2015, 4256, 4358
BstBI	TTCGAA	1 cut(s)	4992
BstDSI	CCRYGG	5 cut(s)	333, 644, 2383, 3270, 5086
BstEII	GGTNACC	1 cut(s)	767
BstENI	CCTNNNNNAGG	1 cut(s)	3440
BstFNI	CGCG	1 cut(s)	5088
BstH2I	RGCGCY	2 cut(s)	2899, 2977
BstHHI	GCGC	3 cut(s)	2898, 2976, 4204
BstMAI	GTCTC	9 cut(s)	1043, 1587, 2150, 3365, 3660, 4700, 4781, 4977, 5409
BstMCI	CGRYCG	1 cut(s)	696
BstNI	CCWGG	9 cut(s)	405, 489, 686, 866, 879, 1956, 2402, 4824, 5284
BstNSI	RCATGY	6 cut(s)	79, 473, 662, 723, 1565, 1685
BstPI	GGTNACC	1 cut(s)	767
BstSFI	CTRYAG	4 cut(s)	168, 177, 1035, 5318
BstSLI	GKGCMC	3 cut(s)	3331, 3643, 3907
BstSNI	TACGTA	2 cut(s)	136, 272
BstUI	CGCG	1 cut(s)	5088
BstV2I	GAAGAC	3 cut(s)	107, 354, 2084
BstXI	CCANNNNNNTGG	2 cut(s)	1497, 4550
BstZ17I	GTATAC	1 cut(s)	4041
Bsu15I	ATCGAT	1 cut(s)	5565
Bsu36I	CCTNAGG	2 cut(s)	4857, 5033
BsuI	GTATCC	2 cut(s)	8, 3268
BsuTUI	ATCGAT	1 cut(s)	5565
BtgI	CCRYGG	5 cut(s)	333, 644, 2383, 3270, 5086
BtgZI	GCGATG	1 cut(s)	1838
BtsI	GCAGTG	2 cut(s)	994, 3467
BtsIMutI	CAGTG	9 cut(s)	994, 1143, 1200, 2872, 3467, 3641, 3859, 3925, 4212
BveI	ACCTGC	4 cut(s)	184, 1174, 2275, 3848
CaiI	CAGNNNCTG	6 cut(s)	176, 1118, 1202, 1471, 5289, 5744
CciI	TCATGA	5 cut(s)	1534, 2131, 3667, 4840, 5395
CfoI	GCGC	3 cut(s)	2898, 2976, 4204
Cfr10I	RCCGGY	1 cut(s)	3417
Cfr42I	CCGCGG	1 cut(s)	5089
ClaI	ATCGAT	1 cut(s)	5565
CseI	GACGC	4 cut(s)	3959, 4717, 4810, 5474
CspCI	CAANNNNNGTGG	6 cut(s)	1339, 1374, 1700, 1735, 5004, 5039
DraI	TTTAAA	3 cut(s)	1444, 1480, 1801
DraIII	CACNNNGTG	1 cut(s)	1206
EaeI	YGGCCR	2 cut(s)	330, 336
Eam1104I	CTCTTC	4 cut(s)	2791, 2832, 3615, 4179
EarI	CTCTTC	4 cut(s)	2791, 2832, 3615, 4179
Ecl136II	GAGCTC	2 cut(s)	1589, 5083
Eco105I	TACGTA	2 cut(s)	136, 272
Eco130I	CCWWGG	7 cut(s)	202, 333, 644, 1766, 2383, 3270, 4575
Eco147I	AGGCCT	1 cut(s)	1762
Eco24I	GRGCYC	4 cut(s)	1591, 2743, 3907, 5085
Eco31I	GGTCTC	2 cut(s)	3365, 4977
Eco47I	GGWCC	8 cut(s)	159, 823, 1025, 1952, 4164, 4728, 5128, 5698
Eco53kI	GAGCTC	2 cut(s)	1589, 5083
Eco72I	CACGTG	1 cut(s)	4358
Eco81I	CCTNAGG	2 cut(s)	4857, 5033
Eco88I	CYCGRG	4 cut(s)	557, 1584, 2811, 5337
Eco91I	GGTNACC	1 cut(s)	767
EcoICRI	GAGCTC	2 cut(s)	1589, 5083
EcoNI	CCTNNNNNAGG	1 cut(s)	3440
EcoO109I	RGGNCCY	5 cut(s)	159, 1952, 2405, 3904, 4164
EcoO65I	GGTNACC	1 cut(s)	767
EcoRI	GAATTC	1 cut(s)	4615
EcoRII	CCWGG	9 cut(s)	403, 487, 684, 864, 877, 1954, 2400, 4822, 5282
EcoT14I	CCWWGG	7 cut(s)	202, 333, 644, 1766, 2383, 3270, 4575
EcoT22I	ATGCAT	4 cut(s)	505, 532, 3723, 4392
EcoT38I	GRGCYC	4 cut(s)	1591, 2743, 3907, 5085
ErhI	CCWWGG	7 cut(s)	202, 333, 644, 1766, 2383, 3270, 4575
Esp3I	CGTCTC	2 cut(s)	4700, 5409
FalI	AAGNNNNNCTT	8 cut(s)	1750, 1782, 2736, 2768, 3336, 3368, 4986, 5018
FaqI	GGGAC	3 cut(s)	145, 159, 1804
FauI	CCCGC	5 cut(s)	1737, 2597, 3387, 3894, 5688
FauNDI	CATATG	2 cut(s)	2221, 4236
FbaI	TGATCA	6 cut(s)	385, 946, 3367, 3376, 4225, 5571
FblI	GTMKAC	5 cut(s)	3757, 3847, 4040, 4083, 5361
FriOI	GRGCYC	4 cut(s)	1591, 2743, 3907, 5085
FspAI	RTGCGCAY	1 cut(s)	4203
FspI	TGCGCA	1 cut(s)	4203
GlaI	GCGC	3 cut(s)	2897, 2975, 4203
GsaI	CCCAGC	2 cut(s)	776, 4723
GsuI	CTGGAG	8 cut(s)	1542, 2295, 3545, 3826, 4461, 4569, 4978, 5243
HaeII	RGCGCY	2 cut(s)	2899, 2977
HapII	CCGG	5 cut(s)	3418, 4610, 5116, 5126, 5830
HgaI	GACGC	4 cut(s)	3959, 4717, 4810, 5474
HhaI	GCGC	3 cut(s)	2898, 2976, 4204
Hin1I	GRCGYC	2 cut(s)	1337, 2780
Hin6I	GCGC	3 cut(s)	2896, 2974, 4202
HinP1I	GCGC	3 cut(s)	2896, 2974, 4202
HincII	GTYRAC	1 cut(s)	1099
HindII	GTYRAC	1 cut(s)	1099
HindIII	AAGCTT	4 cut(s)	2452, 4013, 4751, 5270
HpaI	GTTAAC	1 cut(s)	1099
HpaII	CCGG	5 cut(s)	3418, 4610, 5116, 5126, 5830
Hpy99I	CGWCG	1 cut(s)	5490
HpyCH4IV	ACGT	8 cut(s)	135, 271, 1337, 2014, 2780, 4255, 4357, 5402
HpySE526I	ACGT	8 cut(s)	135, 271, 1337, 2014, 2780, 4255, 4357, 5402
Hsp92I	GRCGYC	2 cut(s)	1337, 2780
HspAI	GCGC	3 cut(s)	2896, 2974, 4202
Kpn2I	TCCGGA	2 cut(s)	5125, 5829
Ksp22I	TGATCA	6 cut(s)	385, 946, 3367, 3376, 4225, 5571
KspAI	GTTAAC	1 cut(s)	1099
KspI	CCGCGG	1 cut(s)	5089
MaeII	ACGT	8 cut(s)	135, 271, 1337, 2014, 2780, 4255, 4357, 5402
MfeI	CAATTG	3 cut(s)	2291, 2524, 5717
MlsI	TGGCCA	2 cut(s)	332, 338
MluNI	TGGCCA	2 cut(s)	332, 338
MlyI	GAGTC	8 cut(s)	1589, 1786, 1991, 3063, 3597, 3763, 4926, 5498
Mox20I	TGGCCA	2 cut(s)	332, 338
Mph1103I	ATGCAT	4 cut(s)	505, 532, 3723, 4392
MroI	TCCGGA	2 cut(s)	5125, 5829
MroXI	GAANNNNTTC	2 cut(s)	30, 3232
MscI	TGGCCA	2 cut(s)	332, 338
MslI	CAYNNNNRTG	9 cut(s)	474, 831, 957, 978, 2205, 3036, 4235, 4548, 5069
Msp20I	TGGCCA	2 cut(s)	332, 338
MspA1I	CMGCKG	7 cut(s)	569, 681, 2081, 2360, 5088, 5251, 5545
MspI	CCGG	5 cut(s)	3418, 4610, 5116, 5126, 5830
MunI	CAATTG	3 cut(s)	2291, 2524, 5717
Mva1269I	GAATGC	3 cut(s)	1257, 2573, 4204
MvaI	CCWGG	9 cut(s)	405, 489, 686, 866, 879, 1956, 2402, 4824, 5284
MvnI	CGCG	1 cut(s)	5088
NciI	CCSGG	1 cut(s)	4610
NcoI	CCATGG	4 cut(s)	333, 644, 2383, 3270
NdeI	CATATG	2 cut(s)	2221, 4236
NsbI	TGCGCA	1 cut(s)	4203
NsiI	ATGCAT	4 cut(s)	505, 532, 3723, 4392
NspI	RCATGY	6 cut(s)	79, 473, 662, 723, 1565, 1685
NspV	TTCGAA	1 cut(s)	4992
OliI	CACNNNNGTG	1 cut(s)	978
PaeR7I	CTCGAG	1 cut(s)	1584
PagI	TCATGA	5 cut(s)	1534, 2131, 3667, 4840, 5395
PaqCI	CACCTGC	4 cut(s)	184, 1174, 2275, 3848
PceI	AGGCCT	1 cut(s)	1762
PciI	ACATGT	2 cut(s)	469, 1561
PctI	GAATGC	3 cut(s)	1257, 2573, 4204
PdmI	GAANNNNTTC	2 cut(s)	30, 3232
PflMI	CCANNNNNTGG	2 cut(s)	650, 1436
PfoI	TCCNGGA	1 cut(s)	877
Ple19I	CGATCG	1 cut(s)	696
PleI	GAGTC	8 cut(s)	1588, 1786, 1990, 3063, 3596, 3762, 4926, 5498
PmaCI	CACGTG	1 cut(s)	4358
PmlI	CACGTG	1 cut(s)	4358
PpsI	GAGTC	8 cut(s)	1588, 1786, 1990, 3063, 3596, 3762, 4926, 5498
Ppu21I	YACGTR	5 cut(s)	136, 272, 2015, 4256, 4358
PpuMI	RGGWCCY	3 cut(s)	159, 1952, 4164
PscI	ACATGT	2 cut(s)	469, 1561
PshBI	ATTAAT	2 cut(s)	611, 4269
PsiI	TTATAA	1 cut(s)	3599
Psp124BI	GAGCTC	2 cut(s)	1591, 5085
Psp5II	RGGWCCY	3 cut(s)	159, 1952, 4164
Psp6I	CCWGG	9 cut(s)	403, 487, 684, 864, 877, 1954, 2400, 4822, 5282
PspCI	CACGTG	1 cut(s)	4358
PspEI	GGTNACC	1 cut(s)	767
PspFI	CCCAGC	2 cut(s)	772, 4719
PspGI	CCWGG	9 cut(s)	403, 487, 684, 864, 877, 1954, 2400, 4822, 5282
PspOMI	GGGCCC	1 cut(s)	3903
PspPPI	RGGWCCY	3 cut(s)	159, 1952, 4164
PstI	CTGCAG	2 cut(s)	172, 181
PstNI	CAGNNNCTG	6 cut(s)	176, 1118, 1202, 1471, 5289, 5744
PvuI	CGATCG	1 cut(s)	696
PvuII	CAGCTG	6 cut(s)	569, 681, 2081, 2360, 5251, 5545
RseI	CAYNNNNRTG	9 cut(s)	474, 831, 957, 978, 2205, 3036, 4235, 4548, 5069
SacI	GAGCTC	2 cut(s)	1591, 5085
SacII	CCGCGG	1 cut(s)	5089
ScaI	AGTACT	2 cut(s)	1154, 1541
SchI	GAGTC	8 cut(s)	1589, 1786, 1991, 3063, 3597, 3763, 4926, 5498
SfcI	CTRYAG	4 cut(s)	168, 177, 1035, 5318
Sfr274I	CTCGAG	1 cut(s)	1584
Sfr303I	CCGCGG	1 cut(s)	5089
SfuI	TTCGAA	1 cut(s)	4992
SgrBI	CCGCGG	1 cut(s)	5089
SinI	GGWCC	8 cut(s)	159, 823, 1025, 1952, 4164, 4728, 5128, 5698
SlaI	CTCGAG	1 cut(s)	1584
SmiMI	CAYNNNNRTG	9 cut(s)	474, 831, 957, 978, 2205, 3036, 4235, 4548, 5069
SmlI	CTYRAG	8 cut(s)	618, 1584, 1850, 2699, 3789, 4159, 5174, 5763
SmoI	CTYRAG	8 cut(s)	618, 1584, 1850, 2699, 3789, 4159, 5174, 5763
SnaBI	TACGTA	2 cut(s)	136, 272
SpeI	ACTAGT	1 cut(s)	1451
SseBI	AGGCCT	1 cut(s)	1762
SstI	GAGCTC	2 cut(s)	1591, 5085
StuI	AGGCCT	1 cut(s)	1762
StyI	CCWWGG	7 cut(s)	202, 333, 644, 1766, 2383, 3270, 4575
TaiI	ACGT	8 cut(s)	138, 274, 1340, 2017, 2783, 4258, 4360, 5405
TatI	WGTACW	4 cut(s)	1131, 1152, 1539, 4071
TauI	GCSGC	2 cut(s)	1076, 1823
TscAI	CASTG	9 cut(s)	994, 1150, 1207, 2872, 3467, 3648, 3859, 3932, 4219
TspRI	CASTG	9 cut(s)	994, 1150, 1207, 2872, 3467, 3648, 3859, 3932, 4219
Van91I	CCANNNNNTGG	2 cut(s)	650, 1436
VneI	GTGCAC	2 cut(s)	3327, 3639
VpaK11BI	GGWCC	8 cut(s)	159, 823, 1025, 1952, 4164, 4728, 5128, 5698
VspI	ATTAAT	2 cut(s)	611, 4269
XagI	CCTNNNNNAGG	1 cut(s)	3440
XapI	RAATTY	7 cut(s)	1272, 1626, 1632, 1644, 2627, 4615, 5381
XceI	RCATGY	6 cut(s)	79, 473, 662, 723, 1565, 1685
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1203, 1244, 4373
XhoI	CTCGAG	1 cut(s)	1584
XmiI	GTMKAC	5 cut(s)	3757, 3847, 4040, 4083, 5361
XmnI	GAANNNNTTC	2 cut(s)	30, 3232
ZraI	GACGTC	2 cut(s)	1338, 2781
ZrmI	AGTACT	2 cut(s)	1154, 1541
Zsp2I	ATGCAT	4 cut(s)	505, 532, 3723, 4392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.