Rmu_sc0000859.1_g000002

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000859.1
OG0003698
Physical Location & Seq
Reverse (-)
22888 .. 24004
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
atgggattggaatacacttcaccagcttttgcatctgccatgggcaacatgattccatcgattaccttcatcttagctgttttgtgcaggatggaacagttcgatatttcgaagcttggtacccaagccaagattgggggaactcttgctgcctttgctggtgcaacactcatgaccctctataagggtgttgctgtaattacaacgcacaccaaaagctcccatcaaactgcagctacctcaaaatcatctacagacgcagaatggataaagggctcccttgtccttattgtttcatttagttccttagcagcattctatatcttacagacatcaacaattaaaatgtatccagcaccaatcactcttacatcattgacctgcttatctgggacactgctctcaacaatcatggcagcaattctagatcacaaagcatcttcttggaagttatcatggaacacacttcttgctcctatgtatagtggaattgtgattttcggaatcacagtttatgttcagaccttagtagtacgaacaaaaggcccagttttcatgacagcttttagaccactagctaccattgttgtagctataatgggactactcattttaggagaagcaatatacttgggaagtgttttgggagctttcttgatagttcttggtctgtacgcaactctttggggaaagaaaaaagagaaggagaagaagtcaatggatcatggtgtatctcagcaagctattgagatcaatctagaaaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

255

Amino Acids

27.33

Weight (kDa)

9.45

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 91 - 228 1.3e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 389
Acc65I GGTACC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 119
AclWI GGATC 1 cut(s) 729
AfaI GTAC 3 cut(s) 121, 534, 674
AfiI CCNNNNNNNGG 2 cut(s) 135, 184
AlwI GGATC 1 cut(s) 729
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 4 cut(s) 149, 233, 311, 416
Asp718I GGTACC 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 545
AsuHPI GGTGA 1 cut(s) 12
AsuII TTCGAA 1 cut(s) 110
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 278
BbvI GCAGC 4 cut(s) 136, 245, 323, 428
BccI CCATC 3 cut(s) 64, 85, 231
BciVI GTATCC 1 cut(s) 360
BfaI CTAG 3 cut(s) 425, 575, 758
BfmI CTRYAG 2 cut(s) 231, 252
BfuAI ACCTGC 1 cut(s) 389
BfuI GTATCC 1 cut(s) 360
BisI GCNGC 4 cut(s) 150, 234, 312, 417
BlsI GCNGC 4 cut(s) 151, 235, 313, 418
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 2 cut(s) 121, 277
BmrI ACTGGG 1 cut(s) 542
BmsI GCATC 2 cut(s) 41, 446
BmuI ACTGGG 1 cut(s) 542
Bpu10I CCTNAGC 1 cut(s) 307
Bpu14I TTCGAA 1 cut(s) 110
Bsa29I ATCGAT 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 698, 728
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 184
Bse1I ACTGG 1 cut(s) 548
BseCI ATCGAT 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 2 cut(s) 135, 184
BseMII CTCAG 1 cut(s) 749
BseNI ACTGG 1 cut(s) 548
BseXI GCAGC 4 cut(s) 136, 245, 323, 428
BsgI GTGCAG 1 cut(s) 106
BshFI GGCC 1 cut(s) 546
BshNI GGYRCC 1 cut(s) 119
BshVI ATCGAT 1 cut(s) 59
BslFI GGGAC 2 cut(s) 406, 615
BslI CCNNNNNNNGG 2 cut(s) 135, 184
BsmFI GGGAC 2 cut(s) 406, 615
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 1 cut(s) 546
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 278
Bsp143I GATC 3 cut(s) 427, 721, 750
Bsp19I CCATGG 1 cut(s) 39
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 1 cut(s) 748
BspDI ATCGAT 1 cut(s) 59
BspHI TCATGA 2 cut(s) 171, 555
BspLI GGNNCC 2 cut(s) 121, 277
BspMAI CTGCAG 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 1 cut(s) 729
BspT104I TTCGAA 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 548
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 3 cut(s) 427, 721, 750
BssT1I CCWWGG 1 cut(s) 39
Bst4CI ACNGT 2 cut(s) 99, 511
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 741
BstDEI CTNAG 4 cut(s) 73, 307, 526, 735
BstDSI CCRYGG 1 cut(s) 39
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 96
BstKTI GATC 3 cut(s) 430, 724, 753
BstMBI GATC 3 cut(s) 427, 721, 750
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstSFI CTRYAG 2 cut(s) 231, 252
BstV1I GCAGC 4 cut(s) 136, 245, 323, 428
Bsu15I ATCGAT 1 cut(s) 59
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 1 cut(s) 546
BsuTUI ATCGAT 1 cut(s) 59
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 1 cut(s) 395
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 741
CciI TCATGA 2 cut(s) 171, 555
Cfr13I GGNCC 1 cut(s) 545
ClaI ATCGAT 1 cut(s) 59
CseI GACGC 1 cut(s) 266
Csp6I GTAC 3 cut(s) 120, 533, 673
CviAII CATG 7 cut(s) 40, 49, 172, 412, 456, 556, 725
CviQI GTAC 3 cut(s) 120, 533, 673
DdeI CTNAG 4 cut(s) 73, 307, 526, 735
DpnI GATC 3 cut(s) 429, 723, 752
DpnII GATC 3 cut(s) 427, 721, 750
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 278
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
FaqI GGGAC 2 cut(s) 406, 615
FatI CATG 7 cut(s) 39, 48, 171, 411, 455, 555, 724
Fnu4HI GCNGC 4 cut(s) 150, 234, 312, 417
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 4 cut(s) 150, 234, 312, 417
FspBI CTAG 3 cut(s) 425, 575, 758
GluI GCNGC 4 cut(s) 150, 234, 312, 417
HaeIII GGCC 1 cut(s) 546
HgaI GACGC 1 cut(s) 266
Hin1II CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 2 cut(s) 52, 504
HphI GGTGA 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 503, 522
Hpy188III TCNNGA 5 cut(s) 172, 425, 556, 655, 758
HpyAV CCTTC 2 cut(s) 76, 697
HpyCH4III ACNGT 2 cut(s) 99, 511
HpyCH4V TGCA 4 cut(s) 32, 87, 164, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 4 cut(s) 73, 307, 526, 735
Hsp92II CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
KpnI GGTACC 1 cut(s) 123
Kzo9I GATC 3 cut(s) 427, 721, 750
LmnI GCTCC 4 cut(s) 224, 281, 478, 647
LpnPI CCDG 7 cut(s) 36, 73, 144, 366, 375, 394, 561
Lsp1109I GCAGC 4 cut(s) 136, 245, 323, 428
LweI GCATC 2 cut(s) 41, 446
MaeI CTAG 3 cut(s) 425, 575, 758
MalI GATC 3 cut(s) 429, 723, 752
MboI GATC 3 cut(s) 427, 721, 750
MboII GAAGA 2 cut(s) 432, 721
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 4 cut(s) 198, 339, 420, 489
MnlI CCTC 2 cut(s) 188, 250
MseI TTAA 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 155
NcoI CCATGG 1 cut(s) 39
NdeII GATC 3 cut(s) 427, 721, 750
NlaIII CATG 7 cut(s) 43, 52, 175, 415, 459, 559, 728
NlaIV GGNNCC 2 cut(s) 121, 277
NspV TTCGAA 1 cut(s) 110
PagI TCATGA 2 cut(s) 171, 555
PctI GAATGC 1 cut(s) 314
PfeI GAWTC 2 cut(s) 52, 504
PkrI GCNGC 4 cut(s) 151, 235, 313, 418
PspN4I GGNNCC 2 cut(s) 121, 277
PspPI GGNCC 1 cut(s) 545
PstI CTGCAG 1 cut(s) 235
RsaI GTAC 3 cut(s) 121, 534, 674
RsaNI GTAC 3 cut(s) 120, 533, 673
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 4 cut(s) 150, 234, 312, 417
Sau3AI GATC 3 cut(s) 427, 721, 750
Sau96I GGNCC 1 cut(s) 545
SduI GDGCHC 1 cut(s) 278
SfaNI GCATC 2 cut(s) 41, 446
SfcI CTRYAG 2 cut(s) 231, 252
SfuI TTCGAA 1 cut(s) 110
Sse9I AATT 4 cut(s) 198, 339, 420, 489
SspMI CTAG 3 cut(s) 425, 575, 758
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 2 cut(s) 99, 511
TaqI TCGA 3 cut(s) 59, 102, 110
TasI AATT 4 cut(s) 198, 339, 420, 489
TfiI GAWTC 2 cut(s) 52, 504
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 402
TseI GCWGC 4 cut(s) 149, 233, 311, 416
TspDTI ATGAA 3 cut(s) 58, 285, 544
TspRI CASTG 1 cut(s) 402
XagI CCTNNNNNAGG 1 cut(s) 182
XbaI TCTAGA 2 cut(s) 424, 757
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 3 cut(s) 425, 575, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.