Rmu_sc0000950.1_g000014

importin subunit beta-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000950.1
OG0005587
Physical Location & Seq
Forward (+)
61113 .. 61832
720 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
atgaacaataaagttgctagtgaactagttgagaatgactttaagctagaaggatcaaatgtaaagaattcagaatatgtgcgagatgatgaagttaatccattagagaatgagcatggtgatgggtttaatgataaggaaaatgatgtcaagcaacttattttcaacgagaatttgagcatcttccagtcaagttatttaagtggagggactgttgtctcttccgatatgcatgaattagaagctagccttgaactggatgttggtagcagcaaaccttctctagtctctaaagaccttgattcaaaggaatcaaccaaacctgttggtaattataataatattttcatcatgggttgcaccaggtctcctgacaatttcaaagttgatgaccacatatgtgattcccacagaattgtcattgagctgttgattactttttacttgggatacatagataagcagcgtagcatcggaagagagctagcaaatgaagagaaacctattgagagccaaaaattagctggtctgatacttgagaattctttggatgttaaagaacaacataggaagtctgatgttgttcagtggtggctggcactagacccttctgcgaaacaccagattaaaatgttcttgttacagagcctctcttcccttatatcagatgcttgttcaactacttcacaagtctttacgaaagttgcgcctcagaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
HEAT HEAT_EZ IBN_N

Protein Analysis

239

Amino Acids

26.86

Weight (kDa)

4.84

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBN_N PF03810 161 - 218 2.4e-06 Importin-beta N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AclWI GGATC 1 cut(s) 61
AcsI RAATTY 3 cut(s) 67, 172, 541
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 5 cut(s) 166, 254, 306, 382, 678
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 1 cut(s) 362
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 5 cut(s) 46, 245, 427, 484, 524
AluI AGCT 5 cut(s) 46, 245, 427, 484, 524
Alw26I GTCTC 3 cut(s) 223, 292, 372
AlwI GGATC 1 cut(s) 61
ApeKI GCWGC 2 cut(s) 270, 463
ApoI RAATTY 3 cut(s) 67, 172, 541
AspLEI GCGC 1 cut(s) 709
AsuHPI GGTGA 1 cut(s) 131
AsuNHI GCTAGC 2 cut(s) 245, 484
BbvI GCAGC 2 cut(s) 282, 475
BccI CCATC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 364
BciVI GTATCC 1 cut(s) 443
BcoDI GTCTC 3 cut(s) 223, 292, 372
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 7 cut(s) 18, 26, 47, 246, 284, 485, 602
BfuI GTATCC 1 cut(s) 443
BisI GCNGC 2 cut(s) 271, 464
BlsI GCNGC 2 cut(s) 272, 465
Bme1390I CCNGG 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 364
BmsI GCATC 3 cut(s) 189, 480, 658
BmtI GCTAGC 2 cut(s) 249, 488
BoxI GACNNNNGTC 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 557
BsaI GGTCTC 1 cut(s) 372
BsaXI ACNNNNNCTCC 4 cut(s) 198, 228, 352, 382
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 187, 261
BseBI CCWGG 1 cut(s) 364
BseGI GGATG 2 cut(s) 265, 556
BseLI CCNNNNNNNGG 1 cut(s) 256
BseNI ACTGG 2 cut(s) 187, 261
BseXI GCAGC 2 cut(s) 282, 475
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 3 cut(s) 223, 292, 372
BsmFI GGGAC 1 cut(s) 223
Bso31I GGTCTC 1 cut(s) 372
Bsp143I GATC 1 cut(s) 53
BspOI GCTAGC 2 cut(s) 249, 488
BspPI GGATC 1 cut(s) 61
BspTNI GGTCTC 1 cut(s) 372
BsrI ACTGG 2 cut(s) 187, 261
BssMI GATC 1 cut(s) 53
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 4 cut(s) 226, 472, 489, 658
BstC8I GCNNGC 3 cut(s) 247, 486, 597
BstDEI CTNAG 1 cut(s) 711
BstF5I GGATG 2 cut(s) 265, 556
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 1 cut(s) 56
BstMAI GTCTC 3 cut(s) 223, 292, 372
BstMBI GATC 1 cut(s) 53
BstNI CCWGG 1 cut(s) 364
BstPAI GACNNNNGTC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 362
BstV1I GCAGC 2 cut(s) 282, 475
BsuI GTATCC 1 cut(s) 443
BtsCI GGATG 2 cut(s) 265, 556
BtsIMutI CAGTG 1 cut(s) 593
Cac8I GCNNGC 3 cut(s) 247, 486, 597
CfoI GCGC 1 cut(s) 709
CsiI ACCWGGT 1 cut(s) 362
CviAII CATG 3 cut(s) 116, 233, 352
CviJI RGCY 9 cut(s) 46, 245, 249, 427, 484, 513, 524, 595, 648
CviKI_1 RGCY 9 cut(s) 46, 245, 249, 427, 484, 513, 524, 595, 648
DdeI CTNAG 1 cut(s) 711
DpnI GATC 1 cut(s) 55
DpnII GATC 1 cut(s) 53
Eam1104I CTCTTC 4 cut(s) 226, 472, 489, 658
EarI CTCTTC 4 cut(s) 226, 472, 489, 658
Eco31I GGTCTC 1 cut(s) 372
EcoRI GAATTC 2 cut(s) 67, 541
EcoRII CCWGG 1 cut(s) 362
EcoT22I ATGCAT 1 cut(s) 234
FaeI CATG 3 cut(s) 119, 236, 355
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 1 cut(s) 223
FatI CATG 3 cut(s) 115, 232, 351
FauNDI CATATG 1 cut(s) 398
Fnu4HI GCNGC 2 cut(s) 271, 464
FokI GGATG 2 cut(s) 272, 563
Fsp4HI GCNGC 2 cut(s) 271, 464
FspBI CTAG 7 cut(s) 18, 26, 47, 246, 284, 485, 602
GlaI GCGC 1 cut(s) 708
GluI GCNGC 2 cut(s) 271, 464
HhaI GCGC 1 cut(s) 709
Hin1II CATG 3 cut(s) 119, 236, 355
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HinfI GANTC 3 cut(s) 302, 311, 404
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 7 cut(s) 73, 226, 476, 531, 577, 667, 714
Hpy188III TCNNGA 1 cut(s) 371
Hpy8I GTNNAC 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 44, 288, 618
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4V TGCA 2 cut(s) 232, 360
HpyF3I CTNAG 1 cut(s) 711
Hsp92II CATG 3 cut(s) 119, 236, 355
HspAI GCGC 1 cut(s) 707
Kzo9I GATC 1 cut(s) 53
LpnPI CCDG 9 cut(s) 200, 242, 336, 349, 376, 384, 510, 581, 635
Lsp1109I GCAGC 2 cut(s) 282, 475
LweI GCATC 3 cut(s) 189, 480, 658
MabI ACCWGGT 1 cut(s) 362
MaeI CTAG 7 cut(s) 18, 26, 47, 246, 284, 485, 602
MaeIII GTNAC 1 cut(s) 639
MalI GATC 1 cut(s) 55
MboI GATC 1 cut(s) 53
MboII GAAGA 5 cut(s) 175, 213, 489, 506, 645
MluCI AATT 8 cut(s) 67, 172, 236, 331, 376, 414, 518, 541
MnlI CCTC 3 cut(s) 200, 659, 720
Mph1103I ATGCAT 1 cut(s) 234
MseI TTAA 6 cut(s) 42, 96, 129, 200, 555, 627
MslI CAYNNNNRTG 2 cut(s) 120, 399
MspR9I CCNGG 1 cut(s) 364
MvaI CCWGG 1 cut(s) 364
NdeI CATATG 1 cut(s) 398
NdeII GATC 1 cut(s) 53
NheI GCTAGC 2 cut(s) 245, 484
NlaIII CATG 3 cut(s) 119, 236, 355
NsiI ATGCAT 1 cut(s) 234
OliI CACNNNNGTG 1 cut(s) 399
PfeI GAWTC 3 cut(s) 302, 311, 404
PkrI GCNGC 2 cut(s) 272, 465
PshAI GACNNNNGTC 1 cut(s) 215
PsiI TTATAA 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
RseI CAYNNNNRTG 2 cut(s) 120, 399
SaqAI TTAA 6 cut(s) 42, 96, 129, 200, 555, 627
SatI GCNGC 2 cut(s) 271, 464
Sau3AI GATC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 364
SexAI ACCWGGT 1 cut(s) 362
SfaNI GCATC 3 cut(s) 189, 480, 658
SmiMI CAYNNNNRTG 2 cut(s) 120, 399
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 8 cut(s) 67, 172, 236, 331, 376, 414, 518, 541
SspI AATATT 1 cut(s) 343
SspMI CTAG 7 cut(s) 18, 26, 47, 246, 284, 485, 602
StyD4I CCNGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 214
TasI AATT 8 cut(s) 67, 172, 236, 331, 376, 414, 518, 541
TfiI GAWTC 3 cut(s) 302, 311, 404
Tru1I TTAA 6 cut(s) 42, 96, 129, 200, 555, 627
Tru9I TTAA 6 cut(s) 42, 96, 129, 200, 555, 627
TscAI CASTG 1 cut(s) 593
TseI GCWGC 2 cut(s) 270, 463
TspDTI ATGAA 5 cut(s) 17, 105, 249, 337, 507
TspRI CASTG 1 cut(s) 593
XapI RAATTY 3 cut(s) 67, 172, 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XspI CTAG 7 cut(s) 18, 26, 47, 246, 284, 485, 602
Zsp2I ATGCAT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.