Rh4BG173200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005587
Physical Location & Seq
Reverse (-)
30919307 .. 30921471
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG173200.1

Sequence Viewer

Length: 1296 bp
ATGGAGAAGGAGTGTTGCCCTTTGCAAGTGCTTAGAGATGATGGTGATTGGACCAGAGACCAATTCTGGGCTGTCATCAGATTCCTCATACATGCTTCCAGGCCCAAGGAAATCTTGCAGTTGTTTGATATATGGAGGAACATTGAGAAATCGCGGATTAATGAATTCAACTACAGCAAGATAATAGGATTGTTAGTTGAGGAGGACCTGATTGAAGAGGCAGTGCTGTGCTTTCAGGATATGAAGAGTCATGGTCTTAGGCTGTGTGTGGAAGTATACAATTCAATTATTCATGGTCTTTCGAGAAAAGGAAACTTTGATGATGCCGATCTCTTCCTTAACGAGATGAAAGAAATGAATTTGGCACCAGACACTGATACTTATGATGGGCTCATTGAGGCTTATGGAAAATATAAAATGTATGATGAGATGGGTATGTGTCTGAAGAAAATGAGATTAAATGGCTGTTCACCGGACCAGATTACCTATAATTTGCTTATTCGAGAGTTTGCACGTGGAGGGTTACTGAAAAGGATGGAAAGAGTCTATCAATCCATGGTTTCAAAAAGAATGGATTTGCAGGCTCCTACTTTGATTGCAATGCTGGAAGTTTATGCAAAATTTGGGATCTTGGAGAAGATGGAAGTGTTTTATAGAAGAGTTTTGAACTCGAGAGCAATTTTAAAGGAGGATTTGATTAAGAAAGTTGCAGAAGTTTATATTGAGAACTATATGTTTTCCAGATTAGAGAACTTGGGAGTTGATCTTTCTCCAAGATTTGGTCAGACTGACCTCGTTTGGTGTGTGCGTCTCCTTTCTCATGCTGGTATTTTGAGTCGAAGAGGTATGGATTCCGTCATTCTAGAGATGGAAGAAAAAGGTGTCCCATGGAATGCAACGGTGGCAAACATAATAATGCTAGCTTATTTGAAGATGAAAGATTTCACCCGCCTGAGAATCATGTTCTCCCAATCACTAACCCATGGTGTGGAGCCCGATATGATCACTGTTGGAATTCTATTTGATGCAAATAGGATTGGCTATGATGGGTCTGCGACTTTAGATGCATGGAGGAAGCAGGGTTTTTTTTACAAAGCAGTTGAAATGAACACCGATCCTCTCGTTATTACTACATTTGGGAAGGGGCATTTCCTCAGGAACTGTGAAGCAGCATACTCCTCTCTTGAACCTGAAGTTAGAGAAAAGAAAACATGGACTTACCAAGATCTCATTGATTCAGTGTTCAAAGACAATCAGTGTAACCTCAGTAAGAGGGACTTAATGAACTCAAGATAA

Protein Analysis

431

Amino Acids

50.18

Weight (kDa)

6.01

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 39 - 193 9.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 78 - 137 3.1e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 89 - 136 9.8e-12 PPR repeat family
PPR PF01535 92 - 120 2.6e-06 PPR repeat
PPR_2 PF13041 130 - 172 1.7e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 364
AccB7I CCANNNNNTGG 2 cut(s) 779, 988
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 154
AciI CCGC 2 cut(s) 154, 949
AclWI GGATC 2 cut(s) 635, 1109
AcsI RAATTY 4 cut(s) 164, 358, 620, 1014
AcuI CTGAAG 2 cut(s) 464, 1212
AcvI CACGTG 1 cut(s) 515
AfiI CCNNNNNNNGG 3 cut(s) 67, 779, 988
AgsI TTSAA 9 cut(s) 169, 215, 285, 564, 667, 931, 1103, 1187, 1246
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 1 cut(s) 923
AluI AGCT 1 cut(s) 923
Alw26I GTCTC 2 cut(s) 51, 815
AlwI GGATC 2 cut(s) 635, 1109
AlwNI CAGNNNCTG 2 cut(s) 374, 1161
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 1 cut(s) 1169
ApoI RAATTY 4 cut(s) 164, 358, 620, 1014
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 941
AspS9I GGNCC 4 cut(s) 51, 102, 205, 475
AsuHPI GGTGA 3 cut(s) 56, 462, 937
AsuNHI GCTAGC 1 cut(s) 919
AvaI CYCGRG 1 cut(s) 670
AvaII GGWCC 3 cut(s) 51, 205, 475
AxyI CCTNAGG 1 cut(s) 1154
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 2 cut(s) 393, 996
BbrPI CACGTG 1 cut(s) 515
BbvI GCAGC 1 cut(s) 1181
BccI CCATC 7 cut(s) 35, 380, 424, 529, 634, 862, 1040
BciT130I CCWGG 1 cut(s) 100
BclI TGATCA 1 cut(s) 1002
BcoDI GTCTC 2 cut(s) 51, 815
BfaI CTAG 2 cut(s) 863, 920
BfmI CTRYAG 1 cut(s) 172
BglII AGATCT 1 cut(s) 1225
BisI GCNGC 1 cut(s) 1170
BlsI GCNGC 1 cut(s) 1171
Bme1390I CCNGG 1 cut(s) 100
Bme18I GGWCC 3 cut(s) 51, 205, 475
BmeT110I CYCGRG 1 cut(s) 670
BmgT120I GGNCC 4 cut(s) 51, 102, 205, 475
BmiI GGNNCC 3 cut(s) 366, 585, 993
BmrFI CCNGG 1 cut(s) 100
BmsI GCATC 3 cut(s) 313, 1015, 1054
BmtI GCTAGC 1 cut(s) 923
BpuEI CTTGAG 1 cut(s) 1273
BsaAI YACGTR 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 4 cut(s) 105, 555, 887, 982
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 779, 988
Bse21I CCTNAGG 1 cut(s) 1154
Bse3DI GCAATG 1 cut(s) 606
Bse8I GATNNNNATC 1 cut(s) 327
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 4 cut(s) 105, 555, 887, 982
BseGI GGATG 1 cut(s) 540
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 3 cut(s) 67, 779, 988
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 3 cut(s) 944, 1168, 1279
BseRI GAGGAG 2 cut(s) 215, 1168
BseXI GCAGC 1 cut(s) 1181
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 364
BsiHKCI CYCGRG 1 cut(s) 670
BsiSI CCGG 1 cut(s) 473
BslFI GGGAC 2 cut(s) 869, 1289
BslI CCNNNNNNNGG 3 cut(s) 67, 779, 988
BsmAI GTCTC 2 cut(s) 51, 815
BsmBI CGTCTC 1 cut(s) 815
BsmFI GGGAC 2 cut(s) 869, 1289
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 1 cut(s) 103
Bso31I GGTCTC 1 cut(s) 51
BsoBI CYCGRG 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 393, 996
Bsp143I GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
Bsp19I CCATGG 3 cut(s) 555, 887, 982
BspACI CCGC 2 cut(s) 154, 949
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 3 cut(s) 945, 1167, 1278
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 366, 585, 993
BspOI GCTAGC 1 cut(s) 923
BspPI GGATC 2 cut(s) 635, 1109
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 606
BssECI CCNNGG 4 cut(s) 105, 555, 887, 982
BssMI GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
BssNAI GTATAC 1 cut(s) 277
BssT1I CCWWGG 4 cut(s) 105, 555, 887, 982
Bst1107I GTATAC 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 100
Bst4CI ACNGT 3 cut(s) 901, 1009, 1163
Bst6I CTCTTC 5 cut(s) 210, 239, 338, 652, 835
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 582, 921
BstDEI CTNAG 5 cut(s) 32, 257, 953, 1154, 1265
BstDSI CCRYGG 3 cut(s) 555, 887, 982
BstF5I GGATG 1 cut(s) 540
BstFNI CGCG 1 cut(s) 154
BstKTI GATC 6 cut(s) 331, 630, 766, 1005, 1117, 1228
BstMAI GTCTC 2 cut(s) 51, 815
BstMBI GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
BstMWI GCNNNNNNNGC 1 cut(s) 902
BstNI CCWGG 1 cut(s) 100
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 172
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 1181
BstX2I RGATCY 2 cut(s) 627, 1225
BstYI RGATCY 2 cut(s) 627, 1225
BstZ17I GTATAC 1 cut(s) 277
Bsu36I CCTNAGG 1 cut(s) 1154
BsuRI GGCC 1 cut(s) 103
BtgI CCRYGG 3 cut(s) 555, 887, 982
BtsCI GGATG 1 cut(s) 540
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 5 cut(s) 228, 372, 1005, 1245, 1262
Cac8I GCNNGC 2 cut(s) 582, 921
CaiI CAGNNNCTG 2 cut(s) 374, 1161
Cfr13I GGNCC 4 cut(s) 51, 102, 205, 475
CseI GACGC 1 cut(s) 797
DdeI CTNAG 5 cut(s) 32, 257, 953, 1154, 1265
DpnI GATC 6 cut(s) 330, 629, 765, 1004, 1116, 1227
DpnII GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
DraI TTTAAA 1 cut(s) 684
Eam1104I CTCTTC 5 cut(s) 210, 239, 338, 652, 835
EarI CTCTTC 5 cut(s) 210, 239, 338, 652, 835
Eco130I CCWWGG 4 cut(s) 105, 555, 887, 982
Eco24I GRGCYC 2 cut(s) 393, 996
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 3 cut(s) 51, 205, 475
Eco57I CTGAAG 2 cut(s) 464, 1212
Eco72I CACGTG 1 cut(s) 515
Eco81I CCTNAGG 1 cut(s) 1154
Eco88I CYCGRG 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 205
EcoRI GAATTC 2 cut(s) 164, 1014
EcoRII CCWGG 1 cut(s) 98
EcoT14I CCWWGG 4 cut(s) 105, 555, 887, 982
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 2 cut(s) 393, 996
ErhI CCWWGG 4 cut(s) 105, 555, 887, 982
Esp3I CGTCTC 1 cut(s) 815
FalI AAGNNNNNCTT 4 cut(s) 98, 130, 1262, 1294
FaqI GGGAC 2 cut(s) 869, 1289
FauI CCCGC 1 cut(s) 956
FbaI TGATCA 1 cut(s) 1002
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 1170
FokI GGATG 1 cut(s) 547
FriOI GRGCYC 2 cut(s) 393, 996
Fsp4HI GCNGC 1 cut(s) 1170
FspBI CTAG 2 cut(s) 863, 920
GluI GCNGC 1 cut(s) 1170
HaeIII GGCC 1 cut(s) 103
HapII CCGG 1 cut(s) 473
HgaI GACGC 1 cut(s) 797
HinfI GANTC 7 cut(s) 81, 247, 543, 835, 851, 957, 1235
HpaII CCGG 1 cut(s) 473
HphI GGTGA 3 cut(s) 56, 462, 937
Hpy166II GTNNAC 2 cut(s) 277, 470
Hpy188I TCNGA 3 cut(s) 80, 444, 786
Hpy188III TCNNGA 9 cut(s) 236, 303, 503, 672, 741, 863, 1156, 1184, 1290
Hpy8I GTNNAC 2 cut(s) 277, 470
HpyAV CCTTC 1 cut(s) 1135
HpyCH4III ACNGT 3 cut(s) 901, 1009, 1163
HpyCH4IV ACGT 1 cut(s) 514
HpyF10VI GCNNNNNNNGC 1 cut(s) 902
HpyF3I CTNAG 5 cut(s) 32, 257, 953, 1154, 1265
HpySE526I ACGT 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 1002
Kzo9I GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
LmnI GCTCC 2 cut(s) 589, 991
Lsp1109I GCAGC 1 cut(s) 1181
LweI GCATC 3 cut(s) 313, 1015, 1054
MaeI CTAG 2 cut(s) 863, 920
MaeII ACGT 1 cut(s) 514
MaeIII GTNAC 2 cut(s) 522, 1259
MalI GATC 6 cut(s) 330, 629, 765, 1004, 1116, 1227
MboI GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
MboII GAAGA 9 cut(s) 227, 256, 325, 457, 649, 669, 852, 884, 943
MflI RGATCY 2 cut(s) 627, 1225
MhlI GDGCHC 2 cut(s) 393, 996
MluCI AATT 9 cut(s) 62, 164, 280, 285, 358, 490, 620, 678, 1014
MlyI GAGTC 3 cut(s) 256, 552, 844
MmeI TCCRAC 1 cut(s) 991
Mph1103I ATGCAT 1 cut(s) 1069
MroXI GAANNNNTTC 1 cut(s) 941
MseI TTAA 6 cut(s) 159, 339, 458, 683, 699, 1280
MslI CAYNNNNRTG 1 cut(s) 914
MspI CCGG 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 100
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 1 cut(s) 100
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 1 cut(s) 902
NcoI CCATGG 3 cut(s) 555, 887, 982
NdeII GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
NheI GCTAGC 1 cut(s) 919
NlaIV GGNNCC 3 cut(s) 366, 585, 993
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 670
PctI GAATGC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 941
PfeI GAWTC 4 cut(s) 81, 851, 957, 1235
PflMI CCANNNNNTGG 2 cut(s) 779, 988
PkrI GCNGC 1 cut(s) 1171
PleI GAGTC 3 cut(s) 255, 551, 843
PmaCI CACGTG 1 cut(s) 515
PmlI CACGTG 1 cut(s) 515
PpsI GAGTC 3 cut(s) 255, 551, 843
Ppu21I YACGTR 1 cut(s) 515
PpuMI RGGWCCY 1 cut(s) 205
PshBI ATTAAT 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 98
PspCI CACGTG 1 cut(s) 515
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 366, 585, 993
PspPI GGNCC 4 cut(s) 51, 102, 205, 475
PspPPI RGGWCCY 1 cut(s) 205
PstNI CAGNNNCTG 2 cut(s) 374, 1161
PsuI RGATCY 2 cut(s) 627, 1225
RseI CAYNNNNRTG 1 cut(s) 914
SaqAI TTAA 6 cut(s) 159, 339, 458, 683, 699, 1280
SatI GCNGC 1 cut(s) 1170
Sau3AI GATC 6 cut(s) 328, 627, 763, 1002, 1114, 1225
Sau96I GGNCC 4 cut(s) 51, 102, 205, 475
SchI GAGTC 3 cut(s) 256, 552, 844
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 2 cut(s) 393, 996
SetI ASST 9 cut(s) 210, 488, 517, 795, 847, 883, 925, 1192, 1266
SfaNI GCATC 3 cut(s) 313, 1015, 1054
SfcI CTRYAG 1 cut(s) 172
Sfr274I CTCGAG 1 cut(s) 670
SinI GGWCC 3 cut(s) 51, 205, 475
SlaI CTCGAG 1 cut(s) 670
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 2 cut(s) 670, 1288
SmoI CTYRAG 2 cut(s) 670, 1288
Sse9I AATT 9 cut(s) 62, 164, 280, 285, 358, 490, 620, 678, 1014
SsiI CCGC 2 cut(s) 154, 949
SspMI CTAG 2 cut(s) 863, 920
StyD4I CCNGG 1 cut(s) 98
StyI CCWWGG 4 cut(s) 105, 555, 887, 982
TaaI ACNGT 3 cut(s) 901, 1009, 1163
TaiI ACGT 1 cut(s) 517
TaqI TCGA 4 cut(s) 302, 502, 671, 838
TasI AATT 9 cut(s) 62, 164, 280, 285, 358, 490, 620, 678, 1014
TfiI GAWTC 4 cut(s) 81, 851, 957, 1235
Tru1I TTAA 6 cut(s) 159, 339, 458, 683, 699, 1280
Tru9I TTAA 6 cut(s) 159, 339, 458, 683, 699, 1280
TscAI CASTG 5 cut(s) 228, 379, 1012, 1245, 1262
TseI GCWGC 1 cut(s) 1169
TspDTI ATGAA 7 cut(s) 177, 257, 281, 362, 371, 950, 1121
TspGWI ACGGA 1 cut(s) 844
TspRI CASTG 5 cut(s) 228, 379, 1012, 1245, 1262
Van91I CCANNNNNTGG 2 cut(s) 779, 988
VpaK11BI GGWCC 3 cut(s) 51, 205, 475
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 4 cut(s) 164, 358, 620, 1014
XbaI TCTAGA 1 cut(s) 862
XceI RCATGY 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 941
XspI CTAG 2 cut(s) 863, 920
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.