Rroxscaffold_1G00043460

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005587
Physical Location & Seq
Reverse (-)
61976094 .. 61976979
886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043460.1

Sequence Viewer

Length: 693 bp
ATGCCAAAGTTAGATGGGTTGTGTAATGCAAGAAGGAACATTGCTGCGGTTATGCGCATTCGTGACAAAGATGATAGAGTTGATAAATCAAATGTGGTTCTTGGTGATAGCAATGCTAGATGGATCAAATGCAAAGAATTCGAATATGTGCGAGATGATGAAGTTGATTCATTAGAGAAGAAGCATAGTGATGGGGTTGATAATAAGGAAAATGATGTCAAGCAACCGGTTTTTGACGAGAAATTGAGCATTTTCCAGTCAAGTTATTTAAGCGGAGTCACCGTTGTCTCATCCCAAATGCATGGTTTGGAAGATAACCTTGAAGCGGATGTCGGCAATATCAAGCCAATGATTAAAAGTGTGCCTATTAAAAGTGAAGCCAATATGTCTCGGTCCGTGATCATTATGTTTAACAACCAAAGTCCGGATAAAATAGGAGCCTCTGCAGATGTTGTGATACCTCGGATAACTAAAATCTATATGGTTGTTTTTCCCGAAGGACATGAATCTTATGATAAATCCGGTGAGTCTTTACAAGTGAACCTTGAGGCTAGTGATGCTGAATTAAATCGCACTACCCACTTACTTGGTCCGGATGATAATGAAAAAGAAGGAAACCGTGAGGGGAGTGGAGAGTCCGGAAACCACAGCAGAAAGGGATTCACGAAGAAAATCTTGTGGCAAATCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

25.74

Weight (kDa)

5.36

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 56
AccIII TCCGGA 3 cut(s) 424, 592, 638
AciI CCGC 3 cut(s) 47, 273, 326
AclWI GGATC 1 cut(s) 131
AcsI RAATTY 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 325, 424
AgeI ACCGGT 1 cut(s) 226
AgsI TTSAA 1 cut(s) 323
Alw26I GTCTC 2 cut(s) 292, 393
AlwI GGATC 1 cut(s) 131
Aor13HI TCCGGA 3 cut(s) 424, 592, 638
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 1 cut(s) 137
AsiGI ACCGGT 1 cut(s) 226
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 2 cut(s) 393, 590
AsuHPI GGTGA 3 cut(s) 116, 271, 536
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 2 cut(s) 393, 590
BbvI GCAGC 1 cut(s) 31
BccI CCATC 3 cut(s) 8, 114, 185
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BclI TGATCA 1 cut(s) 399
BcoDI GTCTC 2 cut(s) 292, 393
BfaI CTAG 2 cut(s) 117, 552
BfmI CTRYAG 2 cut(s) 444, 689
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme18I GGWCC 2 cut(s) 393, 590
BmgT120I GGNCC 2 cut(s) 393, 590
BmiI GGNNCC 1 cut(s) 439
BmsI GCATC 1 cut(s) 547
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 566
BsaJI CCNNGG 1 cut(s) 461
BsaWI WCCGGW 5 cut(s) 226, 424, 521, 592, 638
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 424
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 2 cut(s) 39, 118
BseAI TCCGGA 3 cut(s) 424, 592, 638
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 3 cut(s) 290, 334, 601
BseLI CCNNNNNNNGG 2 cut(s) 325, 424
BseMI GCAATG 2 cut(s) 39, 118
BseNI ACTGG 1 cut(s) 256
BseXI GCAGC 1 cut(s) 31
BshTI ACCGGT 1 cut(s) 226
BsiSI CCGG 5 cut(s) 227, 425, 522, 593, 639
BslI CCNNNNNNNGG 2 cut(s) 325, 424
BsmAI GTCTC 2 cut(s) 292, 393
BsmI GAATGC 1 cut(s) 57
Bsp119I TTCGAA 1 cut(s) 141
Bsp13I TCCGGA 3 cut(s) 424, 592, 638
Bsp143I GATC 2 cut(s) 123, 399
BspACI CCGC 3 cut(s) 47, 273, 326
BspEI TCCGGA 3 cut(s) 424, 592, 638
BspLI GGNNCC 1 cut(s) 439
BspMAI CTGCAG 1 cut(s) 448
BspPI GGATC 1 cut(s) 131
BspT104I TTCGAA 1 cut(s) 141
BsrDI GCAATG 2 cut(s) 39, 118
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 2 cut(s) 123, 399
Bst4CI ACNGT 2 cut(s) 283, 620
BstBI TTCGAA 1 cut(s) 141
BstF5I GGATG 3 cut(s) 290, 334, 601
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 126, 402
BstMAI GTCTC 2 cut(s) 292, 393
BstMBI GATC 2 cut(s) 123, 399
BstMWI GCNNNNNNNGC 1 cut(s) 557
BstSFI CTRYAG 2 cut(s) 444, 689
BstV1I GCAGC 1 cut(s) 31
BstXI CCANNNNNNTGG 2 cut(s) 302, 587
BtsCI GGATG 3 cut(s) 290, 334, 601
CfoI GCGC 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 393, 590
CpoI CGGWCCG 1 cut(s) 393
CspAI ACCGGT 1 cut(s) 226
CspI CGGWCCG 1 cut(s) 393
CviAII CATG 2 cut(s) 302, 503
CviJI RGCY 4 cut(s) 346, 380, 440, 551
CviKI_1 RGCY 4 cut(s) 346, 380, 440, 551
DpnI GATC 2 cut(s) 125, 401
DpnII GATC 2 cut(s) 123, 399
Eco47I GGWCC 2 cut(s) 393, 590
EcoRI GAATTC 1 cut(s) 137
EcoT22I ATGCAT 1 cut(s) 303
FaeI CATG 2 cut(s) 305, 506
FalI AAGNNNNNCTT 6 cut(s) 303, 335, 528, 560, 659, 691
FatI CATG 2 cut(s) 301, 502
FbaI TGATCA 1 cut(s) 399
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 3 cut(s) 277, 341, 608
Fsp4HI GCNGC 1 cut(s) 45
FspAI RTGCGCAY 1 cut(s) 56
FspBI CTAG 2 cut(s) 117, 552
FspI TGCGCA 1 cut(s) 56
GlaI GCGC 1 cut(s) 56
GluI GCNGC 1 cut(s) 45
HapII CCGG 5 cut(s) 227, 425, 522, 593, 639
HhaI GCGC 1 cut(s) 57
Hin1II CATG 2 cut(s) 305, 506
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HinfI GANTC 6 cut(s) 167, 276, 506, 527, 635, 660
HpaII CCGG 5 cut(s) 227, 425, 522, 593, 639
HphI GGTGA 3 cut(s) 116, 271, 536
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 1 cut(s) 465
Hpy188III TCNNGA 6 cut(s) 62, 425, 494, 593, 639, 664
Hpy8I GTNNAC 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 27, 491, 605
HpyCH4III ACNGT 2 cut(s) 283, 620
HpyCH4V TGCA 4 cut(s) 29, 132, 301, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 557
Hsp92II CATG 2 cut(s) 305, 506
HspAI GCGC 1 cut(s) 55
Kpn2I TCCGGA 3 cut(s) 424, 592, 638
Ksp22I TGATCA 1 cut(s) 399
Kzo9I GATC 2 cut(s) 123, 399
LmnI GCTCC 1 cut(s) 437
LpnPI CCDG 6 cut(s) 240, 269, 438, 535, 606, 652
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 547
MaeI CTAG 2 cut(s) 117, 552
MaeIII GTNAC 2 cut(s) 62, 277
MalI GATC 2 cut(s) 125, 401
MboI GATC 2 cut(s) 123, 399
MboII GAAGA 3 cut(s) 190, 323, 679
MluCI AATT 3 cut(s) 137, 242, 563
MlyI GAGTC 3 cut(s) 285, 536, 644
MnlI CCTC 4 cut(s) 451, 471, 541, 616
Mph1103I ATGCAT 1 cut(s) 303
MroI TCCGGA 3 cut(s) 424, 592, 638
MseI TTAA 5 cut(s) 269, 354, 369, 411, 566
MslI CAYNNNNRTG 1 cut(s) 189
MspI CCGG 5 cut(s) 227, 425, 522, 593, 639
Mva1269I GAATGC 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 557
NdeII GATC 2 cut(s) 123, 399
NlaIII CATG 2 cut(s) 305, 506
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 2 cut(s) 62, 277
NsbI TGCGCA 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 303
NspV TTCGAA 1 cut(s) 141
PctI GAATGC 1 cut(s) 57
PfeI GAWTC 3 cut(s) 167, 506, 660
PinAI ACCGGT 1 cut(s) 226
PkrI GCNGC 1 cut(s) 46
PleI GAGTC 3 cut(s) 284, 535, 643
PpsI GAGTC 3 cut(s) 284, 535, 643
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 2 cut(s) 393, 590
PstI CTGCAG 1 cut(s) 448
RseI CAYNNNNRTG 1 cut(s) 189
Rsr2I CGGWCCG 1 cut(s) 393
RsrII CGGWCCG 1 cut(s) 393
SaqAI TTAA 5 cut(s) 269, 354, 369, 411, 566
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 2 cut(s) 123, 399
Sau96I GGNCC 2 cut(s) 393, 590
SchI GAGTC 3 cut(s) 285, 536, 644
SetI ASST 3 cut(s) 321, 463, 546
SfaNI GCATC 1 cut(s) 547
SfcI CTRYAG 2 cut(s) 444, 689
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 2 cut(s) 393, 590
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 3 cut(s) 137, 242, 563
SsiI CCGC 3 cut(s) 47, 273, 326
SspMI CTAG 2 cut(s) 117, 552
TaaI ACNGT 2 cut(s) 283, 620
TaqI TCGA 1 cut(s) 141
TaqII GACCGA 1 cut(s) 381
TasI AATT 3 cut(s) 137, 242, 563
TfiI GAWTC 3 cut(s) 167, 506, 660
Tru1I TTAA 5 cut(s) 269, 354, 369, 411, 566
Tru9I TTAA 5 cut(s) 269, 354, 369, 411, 566
TseFI GTSAC 2 cut(s) 62, 277
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 2 cut(s) 62, 277
TspDTI ATGAA 4 cut(s) 159, 174, 519, 618
TspGWI ACGGA 1 cut(s) 385
VpaK11BI GGWCC 2 cut(s) 393, 590
XapI RAATTY 1 cut(s) 137
XspI CTAG 2 cut(s) 117, 552
Zsp2I ATGCAT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.