Rroxscaffold_4G00290720

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005587
Physical Location & Seq
Reverse (-)
11268340 .. 11269257
918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00290720.1

Sequence Viewer

Length: 483 bp
ATGGGTTGTGTAATGCAAGAAGGAACATTGCTTGCGGTTATGCGCATTCGTGACAAAGATGATAGAGTTGATAAATCAAATGTGGTTCTTGGTGATAGCAATGGTTTACACCCCATTAGATATGAAATGTTGCTAGTGAACCGAGTTGAGAATGGCTTTAAGCTAGATGGATCAAATGCAAAGAATTCAGAATATGTGCGAGATGATGAAGTTGATTCATTAGAGAAGGAGCATAGTGATGGGGTTGATGATAAGGAAAATGATGTCAAGCAACTGTCTTTACAAGTGAACCTTGAGGCTAGTGATGCTGAATTAAATCGCACTACCCACTTACTTGGTCCGGATGATAATGAAAAAGATGGAAACTGTGAGGGGAGTGGAGAGTACGGAAACCACGGCGAAGGGATTCACGAAGAAAATCTTGTGGCAAATCCTATAGTTACTGTTCAAACTACGATCCTTATGAAAATCGGATCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.65

Weight (kDa)

4.5

Isoelectric Point (pI)

3.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 44
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 1 cut(s) 35
AclWI GGATC 3 cut(s) 178, 451, 481
AcsI RAATTY 1 cut(s) 184
AfaI GTAC 1 cut(s) 386
AgsI TTSAA 1 cut(s) 449
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
AlwI GGATC 3 cut(s) 178, 451, 481
Aor13HI TCCGGA 1 cut(s) 340
ApoI RAATTY 1 cut(s) 184
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 104
AvaII GGWCC 1 cut(s) 338
BccI CCATC 3 cut(s) 161, 233, 353
BceAI ACGGC 1 cut(s) 412
BfaI CTAG 4 cut(s) 134, 164, 300, 481
BfmI CTRYAG 1 cut(s) 435
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmsI GCATC 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 394
BsaWI WCCGGW 1 cut(s) 340
Bse3DI GCAATG 2 cut(s) 26, 106
BseAI TCCGGA 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 1 cut(s) 349
BseMI GCAATG 2 cut(s) 26, 106
BsiSI CCGG 1 cut(s) 341
BsmI GAATGC 1 cut(s) 45
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 3 cut(s) 170, 456, 473
BspACI CCGC 1 cut(s) 35
BspEI TCCGGA 1 cut(s) 340
BspPI GGATC 3 cut(s) 178, 451, 481
BsrDI GCAATG 2 cut(s) 26, 106
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 3 cut(s) 170, 456, 473
Bst4CI ACNGT 3 cut(s) 276, 368, 445
BstC8I GCNNGC 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 349
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 3 cut(s) 173, 459, 476
BstMBI GATC 3 cut(s) 170, 456, 473
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstSFI CTRYAG 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 335
BstYI RGATCY 1 cut(s) 473
BtgI CCRYGG 1 cut(s) 394
BtsCI GGATG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 33
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 1 cut(s) 385
CviJI RGCY 3 cut(s) 156, 163, 299
CviKI_1 RGCY 3 cut(s) 156, 163, 299
CviQI GTAC 1 cut(s) 385
DpnI GATC 3 cut(s) 172, 458, 475
DpnII GATC 3 cut(s) 170, 456, 473
Eco47I GGWCC 1 cut(s) 338
EcoRI GAATTC 1 cut(s) 184
FaiI YATR 6 cut(s) 41, 123, 195, 234, 437, 464
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 405, 437
FokI GGATG 1 cut(s) 356
FspAI RTGCGCAY 1 cut(s) 44
FspBI CTAG 4 cut(s) 134, 164, 300, 481
FspI TGCGCA 1 cut(s) 44
GlaI GCGC 1 cut(s) 44
HapII CCGG 1 cut(s) 341
HhaI GCGC 1 cut(s) 45
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 2 cut(s) 215, 406
HpaII CCGG 1 cut(s) 341
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 3 cut(s) 107, 139, 289
Hpy188I TCNGA 2 cut(s) 190, 473
Hpy188III TCNNGA 3 cut(s) 50, 341, 410
Hpy8I GTNNAC 3 cut(s) 107, 139, 289
HpyAV CCTTC 3 cut(s) 14, 220, 395
HpyCH4III ACNGT 3 cut(s) 276, 368, 445
HpyCH4V TGCA 2 cut(s) 16, 179
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HspAI GCGC 1 cut(s) 43
Kpn2I TCCGGA 1 cut(s) 340
Kzo9I GATC 3 cut(s) 170, 456, 473
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 1 cut(s) 354
LweI GCATC 1 cut(s) 295
MaeI CTAG 4 cut(s) 134, 164, 300, 481
MaeIII GTNAC 2 cut(s) 50, 439
MalI GATC 3 cut(s) 172, 458, 475
MboI GATC 3 cut(s) 170, 456, 473
MboII GAAGA 1 cut(s) 425
MflI RGATCY 1 cut(s) 473
MluCI AATT 2 cut(s) 184, 311
MnlI CCTC 2 cut(s) 289, 364
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 2 cut(s) 159, 314
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 1 cut(s) 341
Mva1269I GAATGC 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeII GATC 3 cut(s) 170, 456, 473
NmuCI GTSAC 1 cut(s) 50
NsbI TGCGCA 1 cut(s) 44
PctI GAATGC 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 2 cut(s) 215, 406
PspPI GGNCC 1 cut(s) 338
PsuI RGATCY 1 cut(s) 473
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 2 cut(s) 159, 314
Sau3AI GATC 3 cut(s) 170, 456, 473
Sau96I GGNCC 1 cut(s) 338
SetI ASST 2 cut(s) 165, 294
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 2 cut(s) 184, 311
SsiI CCGC 1 cut(s) 35
SspMI CTAG 4 cut(s) 134, 164, 300, 481
TaaI ACNGT 3 cut(s) 276, 368, 445
TasI AATT 2 cut(s) 184, 311
TfiI GAWTC 2 cut(s) 215, 406
Tru1I TTAA 2 cut(s) 159, 314
Tru9I TTAA 2 cut(s) 159, 314
TseFI GTSAC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 5 cut(s) 138, 207, 222, 366, 479
TspGWI ACGGA 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 338
XapI RAATTY 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 4 cut(s) 134, 164, 300, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.