Rmu_sc0001017.1_g000009

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001017.1
OG0002711
Physical Location & Seq
Forward (+)
47690 .. 49578
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1392 bp
atgagaaaattacaggttgctactattgttatactgggattactattcctgcaagcggcagaatgtagagtagagaatattaaggatgcagttacgtactccgccattaactgccggaagcacagtgctcttttgactgatttcggagccgttggcgatggaaagacattgaacacaaaggcttttaaagatgcagttgataaactaagccagtatgcatctgatggcggagcacagctaattgtgccccccggtaactggcttaccggaagcttcaacctcacaagccactttacccttttcctccataaggatgctgttattcttgggtctcaggatgagtccgagtggcctcttcttccagcactgccttcatacgggcaagggagagaggcccctggtggaaggttaagcagtctaatctttggaacaaacctttccgatgtcgtaattacaggtaacaatggcaccattgatgggcaaggtgctccatggtgggacaaattcaagaagggtcttttgaaagtcacccgaccatacttggttgatattatgtactctaatcagatccaaatctccaatctcacttttcgtaattctccatcctggcacatccatcccatttacagcagtaacattactatacagggcctcacaatccttgcacccgttaccgttcctaacacagatggtattgaccccgactcgtgttcacaaactcgaattgaagactgttacatagtctccggggatgactgcattgcagtgaagagtggctgggatcagcatggaatcaaatttcgaatgccaaccaagcatcttgttattaggaggcttacctgtatttcacctaaaagtgctaccgttgccctaggcagtgaaatgtctggtggaatcagggatgtaagagttgaagatgtgacagccattaacaccgaatccagcattaggatcaaaacatctgtaggcagaggagcttatgtaaaagacatttatgtacgaagaatgacgttgaacactatgaagttcgttttttggatgacaggcgcctatggttcacatccaggcgagggttttgatccaaaggcacttcctgtgattcaaaacatcaactatagagaggttgttgctgagaatgttacaatgccggcccagttggatggaattgctagtgatcctttcaagggaatttgcatctctaatgtgagtatctcactcactgaaaagccaaagaaattgttgtggaactgtactgatgttgagggcgttacgagcaatgtgactccgaaggcttgcgattcgttgcccgagcagcaaaaaccaatcgattgtgctttccctgaggataagttagccattgagggcgttaagctgaccacttgttctgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

463

Amino Acids

50.47

Weight (kDa)

7.05

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 140 - 421 4.1e-35 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 467, 1046
AciI CCGC 3 cut(s) 56, 102, 228
AclWI GGATC 5 cut(s) 562, 789, 959, 1073, 1169
AcsI RAATTY 3 cut(s) 503, 797, 1188
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 4 cut(s) 98, 557, 999, 1252
AfiI CCNNNNNNNGG 8 cut(s) 55, 258, 310, 377, 477, 948, 1070, 1184
AgsI TTSAA 9 cut(s) 172, 277, 508, 523, 728, 914, 1015, 1103, 1183
AjnI CCWGG 3 cut(s) 397, 605, 1063
AluBI AGCT 4 cut(s) 238, 273, 977, 1372
AluI AGCT 4 cut(s) 238, 273, 977, 1372
Alw21I GWGCWC 3 cut(s) 130, 235, 490
Alw26I GTCTC 2 cut(s) 336, 748
AlwI GGATC 5 cut(s) 562, 789, 959, 1073, 1169
Ama87I CYCGRG 1 cut(s) 1307
AoxI GGCC 4 cut(s) 350, 393, 649, 1149
ApeKI GCWGC 1 cut(s) 1312
ApoI RAATTY 3 cut(s) 503, 797, 1188
AspA2I CCTAGG 1 cut(s) 871
AspLEI GCGC 1 cut(s) 1049
AspS9I GGNCC 3 cut(s) 394, 649, 1150
AsuC2I CCSGG 2 cut(s) 252, 748
AsuHPI GGTGA 2 cut(s) 520, 840
AsuII TTCGAA 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 1307
AvrII CCTAGG 1 cut(s) 871
AxyI CCTNAGG 1 cut(s) 1341
BaeGI GKGCMC 1 cut(s) 249
BanI GGYRCC 2 cut(s) 467, 1046
BauI CACGAG 1 cut(s) 706
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 3 cut(s) 130, 235, 490
BbvI GCAGC 1 cut(s) 1324
BccI CCATC 7 cut(s) 152, 218, 470, 610, 624, 683, 1154
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 3 cut(s) 399, 607, 1065
BcnI CCSGG 2 cut(s) 252, 748
BcoDI GTCTC 2 cut(s) 336, 748
BfaI CTAG 2 cut(s) 872, 1170
BfmI CTRYAG 2 cut(s) 963, 1114
BfoI RGCGCY 1 cut(s) 1050
BisI GCNGC 2 cut(s) 57, 1313
BlnI CCTAGG 1 cut(s) 871
BlsI GCNGC 2 cut(s) 58, 1314
Bme1390I CCNGG 5 cut(s) 252, 399, 607, 748, 1065
BmeT110I CYCGRG 1 cut(s) 1307
BmgT120I GGNCC 3 cut(s) 394, 649, 1150
BmiI GGNNCC 4 cut(s) 148, 396, 469, 1048
BmrFI CCNGG 5 cut(s) 252, 399, 607, 748, 1065
BmrI ACTGGG 2 cut(s) 44, 1147
BmsI GCATC 6 cut(s) 76, 181, 227, 304, 826, 1203
BmuI ACTGGG 2 cut(s) 44, 1147
BpiI GAAGAC 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 802
BpuMI CCSGG 2 cut(s) 252, 748
Bsa29I ATCGAT 1 cut(s) 1326
BsaAI YACGTR 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 572
BsaHI GRCGYC 1 cut(s) 1047
BsaI GGTCTC 1 cut(s) 336
BsaJI CCNNGG 5 cut(s) 250, 397, 491, 747, 871
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 4 cut(s) 728, 758, 966, 996
Bsc4I CCNNNNNNNGG 8 cut(s) 55, 258, 310, 377, 477, 948, 1070, 1184
Bse118I RCCGGY 1 cut(s) 1147
Bse1I ACTGG 4 cut(s) 39, 211, 263, 1153
Bse21I CCTNAGG 1 cut(s) 1341
Bse3DI GCAATG 2 cut(s) 759, 1282
Bse8I GATNNNNATC 1 cut(s) 572
BseBI CCWGG 3 cut(s) 399, 607, 1065
BseCI ATCGAT 1 cut(s) 1326
BseDI CCNNGG 5 cut(s) 250, 397, 491, 747, 871
BseJI GATNNNNATC 1 cut(s) 572
BseLI CCNNNNNNNGG 8 cut(s) 55, 258, 310, 377, 477, 948, 1070, 1184
BseMI GCAATG 2 cut(s) 759, 1282
BseMII CTCAG 3 cut(s) 347, 1122, 1332
BseNI ACTGG 4 cut(s) 39, 211, 263, 1153
BseRI GAGGAG 1 cut(s) 987
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 1 cut(s) 1324
BseYI CCCAGC 1 cut(s) 777
BshFI GGCC 4 cut(s) 352, 395, 651, 1151
BshNI GGYRCC 2 cut(s) 467, 1046
BshVI ATCGAT 1 cut(s) 1326
BsiHKAI GWGCWC 3 cut(s) 130, 235, 490
BsiHKCI CYCGRG 1 cut(s) 1307
BsiSI CCGG 5 cut(s) 115, 252, 267, 747, 1148
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 8 cut(s) 55, 258, 310, 377, 477, 948, 1070, 1184
BsmAI GTCTC 2 cut(s) 336, 748
BsmFI GGGAC 1 cut(s) 512
BsmI GAATGC 1 cut(s) 810
BsnI GGCC 4 cut(s) 352, 395, 651, 1151
Bso31I GGTCTC 1 cut(s) 336
BsoBI CYCGRG 1 cut(s) 1307
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 4 cut(s) 130, 235, 249, 490
Bsp143I GATC 5 cut(s) 567, 781, 951, 1078, 1174
Bsp19I CCATGG 1 cut(s) 491
BspACI CCGC 3 cut(s) 56, 102, 228
BspANI GGCC 4 cut(s) 352, 395, 651, 1151
BspCNI CTCAG 3 cut(s) 346, 1123, 1333
BspDI ATCGAT 1 cut(s) 1326
BspLI GGNNCC 4 cut(s) 148, 396, 469, 1048
BspPI GGATC 5 cut(s) 562, 789, 959, 1073, 1169
BspT104I TTCGAA 1 cut(s) 802
BspT107I GGYRCC 2 cut(s) 467, 1046
BspTNI GGTCTC 1 cut(s) 336
BsrDI GCAATG 2 cut(s) 759, 1282
BsrFI RCCGGY 1 cut(s) 1147
BsrI ACTGG 4 cut(s) 39, 211, 263, 1153
BssAI RCCGGY 1 cut(s) 1147
BssECI CCNNGG 5 cut(s) 250, 397, 491, 747, 871
BssMI GATC 5 cut(s) 567, 781, 951, 1078, 1174
BssNI GRCGYC 1 cut(s) 1047
BssSI CACGAG 1 cut(s) 706
BssT1I CCWWGG 2 cut(s) 491, 871
Bst2BI CACGAG 1 cut(s) 706
Bst2UI CCWGG 3 cut(s) 399, 607, 1065
Bst4CI ACNGT 5 cut(s) 125, 676, 734, 865, 1250
Bst6I CTCTTC 2 cut(s) 360, 764
BstACI GRCGYC 1 cut(s) 1047
BstBAI YACGTR 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 3 cut(s) 54, 1149, 1294
BstDEI CTNAG 4 cut(s) 206, 333, 1131, 1341
BstDSI CCRYGG 1 cut(s) 491
BstENI CCTNNNNNAGG 1 cut(s) 308
BstH2I RGCGCY 1 cut(s) 1050
BstHHI GCGC 1 cut(s) 1049
BstKTI GATC 5 cut(s) 570, 784, 954, 1081, 1177
BstMAI GTCTC 2 cut(s) 336, 748
BstMBI GATC 5 cut(s) 567, 781, 951, 1078, 1174
BstMWI GCNNNNNNNGC 5 cut(s) 244, 814, 866, 1272, 1312
BstNI CCWGG 3 cut(s) 399, 607, 1065
BstSCI CCNGG 5 cut(s) 250, 397, 605, 746, 1063
BstSFI CTRYAG 2 cut(s) 963, 1114
BstSLI GKGCMC 1 cut(s) 249
BstSNI TACGTA 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 1324
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 1 cut(s) 567
BstXI CCANNNNNNTGG 1 cut(s) 1160
BstYI RGATCY 1 cut(s) 567
Bsu15I ATCGAT 1 cut(s) 1326
Bsu36I CCTNAGG 1 cut(s) 1341
BsuRI GGCC 4 cut(s) 352, 395, 651, 1151
BsuTUI ATCGAT 1 cut(s) 1326
BtgI CCRYGG 1 cut(s) 491
BtgZI GCGATG 1 cut(s) 171
BtsI GCAGTG 3 cut(s) 365, 771, 883
BtsIMutI CAGTG 5 cut(s) 130, 365, 771, 883, 1218
Cac8I GCNNGC 3 cut(s) 54, 1149, 1294
CfoI GCGC 1 cut(s) 1049
Cfr10I RCCGGY 1 cut(s) 1147
Cfr13I GGNCC 3 cut(s) 394, 649, 1150
ClaI ATCGAT 1 cut(s) 1326
Csp6I GTAC 4 cut(s) 97, 556, 998, 1251
CviAII CATG 2 cut(s) 492, 788
CviQI GTAC 4 cut(s) 97, 556, 998, 1251
DdeI CTNAG 4 cut(s) 206, 333, 1131, 1341
DinI GGCGCC 1 cut(s) 1048
DpnI GATC 5 cut(s) 569, 783, 953, 1080, 1176
DpnII GATC 5 cut(s) 567, 781, 951, 1078, 1174
DraI TTTAAA 1 cut(s) 187
Eam1104I CTCTTC 2 cut(s) 360, 764
EarI CTCTTC 2 cut(s) 360, 764
EciI GGCGGA 2 cut(s) 91, 243
Eco105I TACGTA 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 491, 871
Eco31I GGTCTC 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 1341
Eco88I CYCGRG 1 cut(s) 1307
EcoNI CCTNNNNNAGG 1 cut(s) 308
EcoO109I RGGNCCY 2 cut(s) 394, 649
EcoRII CCWGG 3 cut(s) 397, 605, 1063
EcoT14I CCWWGG 2 cut(s) 491, 871
EcoT22I ATGCAT 1 cut(s) 220
EgeI GGCGCC 1 cut(s) 1048
EheI GGCGCC 1 cut(s) 1048
ErhI CCWWGG 2 cut(s) 491, 871
FaeI CATG 2 cut(s) 495, 791
FaqI GGGAC 1 cut(s) 512
FatI CATG 2 cut(s) 491, 787
Fnu4HI GCNGC 2 cut(s) 57, 1313
Fsp4HI GCNGC 2 cut(s) 57, 1313
FspBI CTAG 2 cut(s) 872, 1170
GlaI GCGC 1 cut(s) 1048
GluI GCNGC 2 cut(s) 57, 1313
GsaI CCCAGC 1 cut(s) 781
HaeII RGCGCY 1 cut(s) 1050
HaeIII GGCC 4 cut(s) 352, 395, 651, 1151
HapII CCGG 5 cut(s) 115, 252, 267, 747, 1148
HhaI GCGC 1 cut(s) 1049
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 2 cut(s) 495, 791
Hin6I GCGC 1 cut(s) 1047
HinP1I GCGC 1 cut(s) 1047
HindIII AAGCTT 1 cut(s) 271
HinfI GANTC 8 cut(s) 341, 704, 792, 894, 938, 1099, 1282, 1298
HpaII CCGG 5 cut(s) 115, 252, 267, 747, 1148
HphI GGTGA 2 cut(s) 520, 840
Hpy166II GTNNAC 2 cut(s) 713, 1058
Hpy188I TCNGA 6 cut(s) 146, 223, 346, 442, 567, 1287
Hpy188III TCNNGA 2 cut(s) 335, 508
Hpy8I GTNNAC 2 cut(s) 713, 1058
HpyAV CCTTC 4 cut(s) 381, 399, 505, 1282
HpyCH4III ACNGT 5 cut(s) 125, 676, 734, 865, 1250
HpyCH4IV ACGT 2 cut(s) 95, 1010
HpyCH4V TGCA 8 cut(s) 52, 89, 194, 218, 665, 759, 764, 1194
HpyF10VI GCNNNNNNNGC 5 cut(s) 244, 814, 866, 1272, 1312
HpyF3I CTNAG 4 cut(s) 206, 333, 1131, 1341
HpySE526I ACGT 2 cut(s) 95, 1010
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 2 cut(s) 495, 791
HspAI GCGC 1 cut(s) 1047
KasI GGCGCC 1 cut(s) 1046
KroI GCCGGC 1 cut(s) 1147
KroNI GCCGGC 1 cut(s) 1149
Kzo9I GATC 5 cut(s) 567, 781, 951, 1078, 1174
LmnI GCTCC 4 cut(s) 146, 230, 493, 974
Lsp1109I GCAGC 1 cut(s) 1324
LweI GCATC 6 cut(s) 76, 181, 227, 304, 826, 1203
MaeI CTAG 2 cut(s) 872, 1170
MaeII ACGT 2 cut(s) 95, 1010
MalI GATC 5 cut(s) 569, 783, 953, 1080, 1176
MboI GATC 5 cut(s) 567, 781, 951, 1078, 1174
MboII GAAGA 6 cut(s) 347, 350, 740, 781, 926, 1014
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 4 cut(s) 130, 235, 249, 490
Mly113I GGCGCC 1 cut(s) 1047
MlyI GAGTC 3 cut(s) 350, 698, 1276
MmeI TCCRAC 1 cut(s) 1137
Mph1103I ATGCAT 1 cut(s) 220
MroNI GCCGGC 1 cut(s) 1147
MseI TTAA 7 cut(s) 81, 108, 186, 410, 930, 1368, 1390
MslI CAYNNNNRTG 2 cut(s) 312, 764
MspI CCGG 5 cut(s) 115, 252, 267, 747, 1148
MspR9I CCNGG 5 cut(s) 252, 399, 607, 748, 1065
Mva1269I GAATGC 1 cut(s) 810
MvaI CCWGG 3 cut(s) 399, 607, 1065
MwoI GCNNNNNNNGC 5 cut(s) 244, 814, 866, 1272, 1312
NaeI GCCGGC 1 cut(s) 1149
NarI GGCGCC 1 cut(s) 1047
NciI CCSGG 2 cut(s) 252, 748
NcoI CCATGG 1 cut(s) 491
NdeII GATC 5 cut(s) 567, 781, 951, 1078, 1174
NgoMIV GCCGGC 1 cut(s) 1147
NlaIII CATG 2 cut(s) 495, 791
NlaIV GGNNCC 4 cut(s) 148, 396, 469, 1048
NmuCI GTSAC 3 cut(s) 526, 919, 1279
NsiI ATGCAT 1 cut(s) 220
NspV TTCGAA 1 cut(s) 802
PctI GAATGC 1 cut(s) 810
PdiI GCCGGC 1 cut(s) 1149
PfeI GAWTC 5 cut(s) 792, 894, 938, 1099, 1298
PkrI GCNGC 2 cut(s) 58, 1314
PleI GAGTC 3 cut(s) 349, 698, 1276
PluTI GGCGCC 1 cut(s) 1050
PpsI GAGTC 3 cut(s) 349, 698, 1276
Ppu21I YACGTR 1 cut(s) 96
Psp6I CCWGG 3 cut(s) 397, 605, 1063
PspFI CCCAGC 1 cut(s) 777
PspGI CCWGG 3 cut(s) 397, 605, 1063
PspN4I GGNNCC 4 cut(s) 148, 396, 469, 1048
PspPI GGNCC 3 cut(s) 394, 649, 1150
PsuI RGATCY 1 cut(s) 567
RsaI GTAC 4 cut(s) 98, 557, 999, 1252
RsaNI GTAC 4 cut(s) 97, 556, 998, 1251
RseI CAYNNNNRTG 2 cut(s) 312, 764
SaqAI TTAA 7 cut(s) 81, 108, 186, 410, 930, 1368, 1390
SatI GCNGC 2 cut(s) 57, 1313
Sau3AI GATC 5 cut(s) 567, 781, 951, 1078, 1174
Sau96I GGNCC 3 cut(s) 394, 649, 1150
SchI GAGTC 3 cut(s) 350, 698, 1276
ScrFI CCNGG 5 cut(s) 252, 399, 607, 748, 1065
SduI GDGCHC 4 cut(s) 130, 235, 249, 490
SfaNI GCATC 6 cut(s) 76, 181, 227, 304, 826, 1203
SfcI CTRYAG 2 cut(s) 963, 1114
SfoI GGCGCC 1 cut(s) 1048
SfuI TTCGAA 1 cut(s) 802
SmiMI CAYNNNNRTG 2 cut(s) 312, 764
SnaBI TACGTA 1 cut(s) 96
SsiI CCGC 3 cut(s) 56, 102, 228
SspDI GGCGCC 1 cut(s) 1046
SspI AATATT 1 cut(s) 79
SspMI CTAG 2 cut(s) 872, 1170
StyD4I CCNGG 5 cut(s) 250, 397, 605, 746, 1063
StyI CCWWGG 2 cut(s) 491, 871
TaaI ACNGT 5 cut(s) 125, 676, 734, 865, 1250
TaiI ACGT 2 cut(s) 98, 1013
TaqI TCGA 3 cut(s) 721, 802, 1326
TatI WGTACW 2 cut(s) 555, 1250
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 5 cut(s) 792, 894, 938, 1099, 1298
Tru1I TTAA 7 cut(s) 81, 108, 186, 410, 930, 1368, 1390
Tru9I TTAA 7 cut(s) 81, 108, 186, 410, 930, 1368, 1390
TscAI CASTG 5 cut(s) 130, 372, 771, 883, 1225
TseFI GTSAC 3 cut(s) 526, 919, 1279
TseI GCWGC 1 cut(s) 1312
Tsp45I GTSAC 3 cut(s) 526, 919, 1279
TspDTI ATGAA 2 cut(s) 363, 1037
TspRI CASTG 5 cut(s) 130, 372, 771, 883, 1225
XagI CCTNNNNNAGG 1 cut(s) 308
XapI RAATTY 3 cut(s) 503, 797, 1188
XmaJI CCTAGG 1 cut(s) 871
XspI CTAG 2 cut(s) 872, 1170
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.