Rmu_sc0001372.1_g000006
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001372.1
OG0003307
Physical Location & Seq
Reverse (-)
38861 .. 40539
1679 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
ctgggtgtcgttttccctcgtgcaagagcagaggagagagatgggttgatttgtacgacacgtcgttgttcggctaacaaaaagcttctagatgagattgcaatacggttgagagactctgatgagcaagtggcagctgttttaacagatgatggttttgatgaacaatatgaacatcatttggaactttttaaggatgaactagagatgttggaagatgaaatggtggaacttgaacaagaattgaaagaacttgaactgttggaggttgcaggagaaatggaacaacttgaaatgttggctattgaaggttcatatccctacttaaacaatggtgtgaaccctgaggcatttgcttatgataaggctggtgatggaaacaaagatatcattctctgggatgttgcacctctcacccttggtatcgaaactgctggtggtattatgactaaattgattcccagaaacactgtgatcccaaccaagaaatcccaggttttcaccacttatcaggatcagcagagtacagtctacattcaggtctatgggggtgaagggagtctaacaagatattgcaggatccttggaaaatttgaacttactggcattcccccagccccaaggggtattcctcggattgaagtcatgtttgaagtggataccaatggtatcctgaatgtcaaggcagaagacaagggcactggcaaatctgagaagatcaccatcaccaatgacaagggttgcacaagccaagaagaaattgagtgcatagtcaaagaggcagaggagttagctgatgaggaccagaaggtgaaggagaggattgatgctcataatagcctgaaaacttttatctacaacttgaagaaccagatcaacgaaatggacaagcttgagtatgatgagaaggataagcttgagaccgctgtgaagaaggccctggaatggatgaaggacaaccagaccgctgagaaggaggactatgaggagaagctcaaggaggttgaagcaatctgcaacgctatcatcacaactgtttaccagaggtcaggtgatactcctgtggattaa

Protein Analysis

356

Amino Acids

40.33

Weight (kDa)

4.53

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 126 - 348 3e-66 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 531
AciI CCGC 2 cut(s) 924, 966
AclWI GGATC 4 cut(s) 469, 522, 574, 587
AcsI RAATTY 1 cut(s) 590
AfaI GTAC 2 cut(s) 55, 526
AfiI CCNNNNNNNGG 1 cut(s) 945
AflIII ACRYGT 1 cut(s) 59
AjiI CACGTC 1 cut(s) 62
AjnI CCWGG 2 cut(s) 492, 939
AluBI AGCT 6 cut(s) 85, 137, 794, 892, 916, 994
AluI AGCT 6 cut(s) 85, 137, 794, 892, 916, 994
Alw26I GTCTC 2 cut(s) 108, 914
AlwI GGATC 4 cut(s) 469, 522, 574, 587
AoxI GGCC 1 cut(s) 936
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 590
AspS9I GGNCC 2 cut(s) 802, 937
AsuHPI GGTGA 8 cut(s) 383, 406, 493, 563, 712, 718, 823, 1064
AvaII GGWCC 1 cut(s) 802
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 701
BaeI ACNNNNGTAYC 1 cut(s) 1047
BamHI GGATCC 1 cut(s) 579
BauI CACGAG 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 696
BbvI GCAGC 1 cut(s) 146
BccI CCATC 4 cut(s) 35, 146, 368, 731
BciT130I CCWGG 2 cut(s) 494, 941
BciVI GTATCC 2 cut(s) 652, 680
BcoDI GTCTC 2 cut(s) 108, 914
BfaI CTAG 2 cut(s) 89, 203
BfuI GTATCC 2 cut(s) 652, 680
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 494, 941
Bme18I GGWCC 1 cut(s) 802
BmgBI CACGTC 1 cut(s) 62
BmgT120I GGNCC 2 cut(s) 802, 937
BmiI GGNNCC 1 cut(s) 581
BmrFI CCNGG 2 cut(s) 494, 941
BmsI GCATC 1 cut(s) 817
BpiI GAAGAC 1 cut(s) 696
BpuEI CTTGAG 3 cut(s) 914, 938, 980
BsaBI GATNNNNATC 1 cut(s) 722
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 6 cut(s) 418, 492, 583, 620, 632, 939
Bsc4I CCNNNNNNNGG 1 cut(s) 945
Bse1I ACTGG 2 cut(s) 607, 706
Bse21I CCTNAGG 1 cut(s) 345
Bse8I GATNNNNATC 1 cut(s) 722
BseBI CCWGG 2 cut(s) 494, 941
BseDI CCNNGG 6 cut(s) 418, 492, 583, 620, 632, 939
BseGI GGATG 3 cut(s) 202, 406, 954
BseJI GATNNNNATC 1 cut(s) 722
BseLI CCNNNNNNNGG 1 cut(s) 945
BseMII CTCAG 3 cut(s) 336, 702, 960
BseNI ACTGG 2 cut(s) 607, 706
BseRI GAGGAG 3 cut(s) 47, 800, 1001
BseSI GKGCMC 1 cut(s) 701
BseXI GCAGC 1 cut(s) 146
BseYI CCCAGC 1 cut(s) 613
BshFI GGCC 1 cut(s) 938
BslI CCNNNNNNNGG 1 cut(s) 945
BsmAI GTCTC 2 cut(s) 108, 914
BsmI GAATGC 1 cut(s) 606
BsnI GGCC 1 cut(s) 938
Bso31I GGTCTC 1 cut(s) 914
Bsp1286I GDGCHC 1 cut(s) 701
Bsp143I GATC 5 cut(s) 474, 514, 579, 717, 873
BspACI CCGC 2 cut(s) 924, 966
BspANI GGCC 1 cut(s) 938
BspCNI CTCAG 3 cut(s) 337, 703, 961
BspLI GGNNCC 1 cut(s) 581
BspPI GGATC 4 cut(s) 469, 522, 574, 587
BspTNI GGTCTC 1 cut(s) 914
BsrI ACTGG 2 cut(s) 607, 706
BssECI CCNNGG 6 cut(s) 418, 492, 583, 620, 632, 939
BssMI GATC 5 cut(s) 474, 514, 579, 717, 873
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 418, 583, 620
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 494, 941
Bst4CI ACNGT 5 cut(s) 108, 261, 472, 529, 1036
BstDEI CTNAG 3 cut(s) 345, 711, 969
BstF5I GGATG 3 cut(s) 202, 406, 954
BstKTI GATC 5 cut(s) 477, 517, 582, 720, 876
BstMAI GTCTC 2 cut(s) 108, 914
BstMBI GATC 5 cut(s) 474, 514, 579, 717, 873
BstNI CCWGG 2 cut(s) 494, 941
BstSCI CCNGG 2 cut(s) 492, 939
BstSLI GKGCMC 1 cut(s) 701
BstV1I GCAGC 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
Bsu36I CCTNAGG 1 cut(s) 345
BsuI GTATCC 2 cut(s) 652, 680
BsuRI GGCC 1 cut(s) 938
BtrI CACGTC 1 cut(s) 62
BtsCI GGATG 3 cut(s) 202, 406, 954
BtsIMutI CAGTG 2 cut(s) 468, 699
Cfr13I GGNCC 2 cut(s) 802, 937
Csp6I GTAC 2 cut(s) 54, 525
CviAII CATG 1 cut(s) 646
CviQI GTAC 2 cut(s) 54, 525
DdeI CTNAG 3 cut(s) 345, 711, 969
DpnI GATC 5 cut(s) 476, 516, 581, 719, 875
DpnII GATC 5 cut(s) 474, 514, 579, 717, 873
Eco130I CCWWGG 3 cut(s) 418, 583, 620
Eco31I GGTCTC 1 cut(s) 914
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 802
Eco81I CCTNAGG 1 cut(s) 345
EcoO109I RGGNCCY 1 cut(s) 937
EcoRII CCWGG 2 cut(s) 492, 939
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 3 cut(s) 418, 583, 620
ErhI CCWWGG 3 cut(s) 418, 583, 620
FaeI CATG 1 cut(s) 649
FatI CATG 1 cut(s) 645
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 3 cut(s) 209, 413, 961
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 2 cut(s) 89, 203
GluI GCNGC 1 cut(s) 135
GsaI CCCAGC 1 cut(s) 617
HaeIII GGCC 1 cut(s) 938
Hin1II CATG 1 cut(s) 649
HindIII AAGCTT 3 cut(s) 83, 890, 914
HinfI GANTC 3 cut(s) 116, 457, 559
HphI GGTGA 8 cut(s) 383, 406, 493, 563, 712, 718, 823, 1064
Hpy166II GTNNAC 3 cut(s) 340, 532, 1039
Hpy188I TCNGA 3 cut(s) 121, 636, 712
Hpy188III TCNNGA 3 cut(s) 89, 512, 673
Hpy8I GTNNAC 3 cut(s) 340, 532, 1039
Hpy99I CGWCG 1 cut(s) 66
HpyAV CCTTC 8 cut(s) 302, 548, 802, 808, 901, 928, 946, 967
HpyCH4III ACNGT 5 cut(s) 108, 261, 472, 529, 1036
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 8 cut(s) 23, 101, 272, 407, 576, 744, 768, 1017
HpyF3I CTNAG 3 cut(s) 345, 711, 969
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 1 cut(s) 649
Kzo9I GATC 5 cut(s) 474, 514, 579, 717, 873
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 1 cut(s) 817
MaeI CTAG 2 cut(s) 89, 203
MaeII ACGT 1 cut(s) 61
MalI GATC 5 cut(s) 476, 516, 581, 719, 875
MboI GATC 5 cut(s) 474, 514, 579, 717, 873
MboII GAAGA 6 cut(s) 227, 701, 727, 767, 877, 943
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 701
MluCI AATT 4 cut(s) 242, 452, 590, 759
MlyI GAGTC 2 cut(s) 110, 568
MmeI TCCRAC 2 cut(s) 192, 243
MseI TTAA 4 cut(s) 143, 192, 326, 1069
MspA1I CMGCKG 3 cut(s) 137, 926, 968
MspR9I CCNGG 2 cut(s) 494, 941
Mva1269I GAATGC 1 cut(s) 606
MvaI CCWGG 2 cut(s) 494, 941
NdeII GATC 5 cut(s) 474, 514, 579, 717, 873
NlaIII CATG 1 cut(s) 649
NlaIV GGNNCC 1 cut(s) 581
PctI GAATGC 1 cut(s) 606
PfeI GAWTC 1 cut(s) 457
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 2 cut(s) 110, 567
PpsI GAGTC 2 cut(s) 110, 567
Psp6I CCWGG 2 cut(s) 492, 939
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 2 cut(s) 492, 939
PspN4I GGNNCC 1 cut(s) 581
PspPI GGNCC 2 cut(s) 802, 937
PsuI RGATCY 1 cut(s) 579
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 2 cut(s) 55, 526
RsaNI GTAC 2 cut(s) 54, 525
SaqAI TTAA 4 cut(s) 143, 192, 326, 1069
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 5 cut(s) 474, 514, 579, 717, 873
Sau96I GGNCC 2 cut(s) 802, 937
SchI GAGTC 2 cut(s) 110, 568
ScrFI CCNGG 2 cut(s) 494, 941
SduI GDGCHC 1 cut(s) 701
SfaNI GCATC 1 cut(s) 817
SinI GGWCC 1 cut(s) 802
SmlI CTYRAG 3 cut(s) 893, 917, 995
SmoI CTYRAG 3 cut(s) 893, 917, 995
Sse9I AATT 4 cut(s) 242, 452, 590, 759
SsiI CCGC 2 cut(s) 924, 966
SspMI CTAG 2 cut(s) 89, 203
StyD4I CCNGG 2 cut(s) 492, 939
StyI CCWWGG 3 cut(s) 418, 583, 620
TaaI ACNGT 5 cut(s) 108, 261, 472, 529, 1036
TaiI ACGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 426
TasI AATT 4 cut(s) 242, 452, 590, 759
TatI WGTACW 1 cut(s) 524
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 4 cut(s) 143, 192, 326, 1069
Tru9I TTAA 4 cut(s) 143, 192, 326, 1069
TscAI CASTG 2 cut(s) 475, 706
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 6 cut(s) 177, 186, 213, 234, 303, 965
TspRI CASTG 2 cut(s) 475, 706
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 1 cut(s) 590
XbaI TCTAGA 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 2 cut(s) 89, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.