Rmu_sc0001849.1_g000001

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001849.1
OG0004496
Physical Location & Seq
Reverse (-)
6894 .. 8210
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
atggctgctgccgctaaacaagtacaacagcaactactagaagatgaaaacgacagattcttcgtcgccgttcatgtcggcgctggataccacgcgccttccaacgagaaggccctcaggtccgccatgaaacgcgcctgcctcgccgccgcttccattctccgccagggctctggtaggtgcatcgatgctgtcactgcagctattcaagtgttggaggtcatagattctagggttggtgtacgtatttcatcacaaaacataactttgactaaggtcacagatcctacaggcatggatggatcatatgaatcttcaatgtgtaatggaagtgactgtaatgaaatgcaatataagggagttcggtatgataagttgtctacagaagatcttagaaggcaggagtttaagactgtggaggaagctgaatcattttacaatgcttatgcaaaggctatgggttttggtgttagaaacgactacaagagattaagtatacgcataacagggagagtcacttcattgcggttggtttgttctgctcaagggaaaagtagtacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

187

Amino Acids

20.51

Weight (kDa)

8.81

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 144 - 185 3.8e-06 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 380, 496
AccII CGCG 2 cut(s) 95, 135
AciI CCGC 6 cut(s) 12, 123, 147, 150, 163, 526
AclWI GGATC 2 cut(s) 278, 310
AfaI GTAC 3 cut(s) 24, 243, 559
AgsI TTSAA 2 cut(s) 209, 318
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 2 cut(s) 203, 425
AluI AGCT 2 cut(s) 203, 425
AlwI GGATC 2 cut(s) 278, 310
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 3 cut(s) 5, 8, 200
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 83, 97, 137
AspS9I GGNCC 2 cut(s) 112, 120
AvaII GGWCC 1 cut(s) 120
AxyI CCTNAGG 1 cut(s) 116
BanII GRGCYC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 212
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 80
BfaI CTAG 2 cut(s) 38, 231
BfmI CTRYAG 3 cut(s) 198, 288, 381
BfoI RGCGCY 1 cut(s) 84
BfuI GTATCC 1 cut(s) 80
BglII AGATCT 1 cut(s) 388
BisI GCNGC 6 cut(s) 6, 9, 12, 147, 150, 201
BlsI GCNGC 6 cut(s) 7, 10, 13, 148, 151, 202
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 2 cut(s) 112, 120
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 2 cut(s) 178, 192
BoxI GACNNNNGTC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 528
Bsa29I ATCGAT 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 166
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 1 cut(s) 521
BseBI CCWGG 1 cut(s) 167
BseCI ATCGAT 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 521
BseMII CTCAG 1 cut(s) 130
BseXI GCAGC 1 cut(s) 212
Bsh1236I CGCG 2 cut(s) 95, 135
BshFI GGCC 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 186
BsnI GGCC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 3 cut(s) 283, 302, 388
BspACI CCGC 6 cut(s) 12, 123, 147, 150, 163, 526
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 1 cut(s) 129
BspDI ATCGAT 1 cut(s) 186
BspFNI CGCG 2 cut(s) 95, 135
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 2 cut(s) 278, 310
BsrDI GCAATG 1 cut(s) 521
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 3 cut(s) 283, 302, 388
BssNAI GTATAC 1 cut(s) 497
Bst1107I GTATAC 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 338, 415
BstBAI YACGTR 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 3 cut(s) 116, 273, 392
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 2 cut(s) 95, 135
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 97, 137
BstKTI GATC 3 cut(s) 286, 305, 391
BstMBI GATC 3 cut(s) 283, 302, 388
BstMWI GCNNNNNNNGC 3 cut(s) 11, 143, 197
BstNI CCWGG 1 cut(s) 167
BstPAI GACNNNNGTC 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 165
BstSFI CTRYAG 3 cut(s) 198, 288, 381
BstSNI TACGTA 1 cut(s) 245
BstUI CGCG 2 cut(s) 95, 135
BstV1I GCAGC 1 cut(s) 212
BstX2I RGATCY 2 cut(s) 283, 388
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 2 cut(s) 283, 388
BstZ17I GTATAC 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 116
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 1 cut(s) 113
BsuTUI ATCGAT 1 cut(s) 186
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 139
CfoI GCGC 3 cut(s) 83, 97, 137
Cfr13I GGNCC 2 cut(s) 112, 120
ClaI ATCGAT 1 cut(s) 186
Csp6I GTAC 3 cut(s) 23, 242, 558
CviAII CATG 4 cut(s) 74, 127, 295, 561
CviJI RGCY 6 cut(s) 5, 113, 171, 203, 425, 455
CviKI_1 RGCY 6 cut(s) 5, 113, 171, 203, 425, 455
CviQI GTAC 3 cut(s) 23, 242, 558
DdeI CTNAG 3 cut(s) 116, 273, 392
DpnI GATC 3 cut(s) 285, 304, 390
DpnII GATC 3 cut(s) 283, 302, 388
EciI GGCGGA 2 cut(s) 112, 152
Eco105I TACGTA 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 120
Eco81I CCTNAGG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 173
FaeI CATG 4 cut(s) 77, 130, 298, 564
FatI CATG 4 cut(s) 73, 126, 294, 560
FauNDI CATATG 1 cut(s) 307
FblI GTMKAC 2 cut(s) 380, 496
Fnu4HI GCNGC 6 cut(s) 6, 9, 12, 147, 150, 201
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 6 cut(s) 6, 9, 12, 147, 150, 201
FspBI CTAG 2 cut(s) 38, 231
GlaI GCGC 3 cut(s) 82, 96, 136
GluI GCNGC 6 cut(s) 6, 9, 12, 147, 150, 201
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 113
HhaI GCGC 3 cut(s) 83, 97, 137
Hin1II CATG 4 cut(s) 77, 130, 298, 564
Hin6I GCGC 3 cut(s) 81, 95, 135
HinP1I GCGC 3 cut(s) 81, 95, 135
HinfI GANTC 5 cut(s) 57, 227, 311, 428, 513
Hpy166II GTNNAC 3 cut(s) 242, 381, 497
Hpy8I GTNNAC 3 cut(s) 242, 381, 497
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 103, 108, 390
HpyCH4III ACNGT 2 cut(s) 338, 415
HpyCH4IV ACGT 1 cut(s) 244
HpyCH4V TGCA 4 cut(s) 183, 200, 349, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 143, 197
HpyF3I CTNAG 3 cut(s) 116, 273, 392
HpySE526I ACGT 1 cut(s) 244
Hsp92II CATG 4 cut(s) 77, 130, 298, 564
HspAI GCGC 3 cut(s) 81, 95, 135
Kzo9I GATC 3 cut(s) 283, 302, 388
LpnPI CCDG 9 cut(s) 69, 103, 151, 152, 159, 179, 276, 386, 492
Lsp1109I GCAGC 1 cut(s) 212
LweI GCATC 2 cut(s) 178, 192
MaeI CTAG 2 cut(s) 38, 231
MaeII ACGT 1 cut(s) 244
MaeIII GTNAC 4 cut(s) 193, 277, 332, 514
MalI GATC 3 cut(s) 285, 304, 390
MboI GATC 3 cut(s) 283, 302, 388
MboII GAAGA 4 cut(s) 52, 53, 306, 398
MflI RGATCY 2 cut(s) 283, 388
MhlI GDGCHC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 522
MmeI TCCRAC 2 cut(s) 126, 195
MnlI CCTC 4 cut(s) 125, 152, 211, 412
MseI TTAA 2 cut(s) 408, 491
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 2 cut(s) 95, 135
MwoI GCNNNNNNNGC 3 cut(s) 11, 143, 197
NdeI CATATG 1 cut(s) 307
NdeII GATC 3 cut(s) 283, 302, 388
NlaIII CATG 4 cut(s) 77, 130, 298, 564
NmuCI GTSAC 4 cut(s) 193, 277, 332, 514
PfeI GAWTC 4 cut(s) 57, 227, 311, 428
PkrI GCNGC 6 cut(s) 7, 10, 13, 148, 151, 202
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 245
PshAI GACNNNNGTC 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspPI GGNCC 2 cut(s) 112, 120
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 2 cut(s) 283, 388
RsaI GTAC 3 cut(s) 24, 243, 559
RsaNI GTAC 3 cut(s) 23, 242, 558
SaqAI TTAA 2 cut(s) 408, 491
SatI GCNGC 6 cut(s) 6, 9, 12, 147, 150, 201
Sau3AI GATC 3 cut(s) 283, 302, 388
Sau96I GGNCC 2 cut(s) 112, 120
SchI GAGTC 1 cut(s) 522
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 1 cut(s) 173
SetI ASST 7 cut(s) 122, 182, 205, 222, 247, 279, 427
SfaNI GCATC 2 cut(s) 178, 192
SfcI CTRYAG 3 cut(s) 198, 288, 381
SinI GGWCC 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
SnaBI TACGTA 1 cut(s) 245
SsiI CCGC 6 cut(s) 12, 123, 147, 150, 163, 526
SspMI CTAG 2 cut(s) 38, 231
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 338, 415
TaiI ACGT 1 cut(s) 247
TaqI TCGA 1 cut(s) 186
TatI WGTACW 2 cut(s) 22, 557
TauI GCSGC 3 cut(s) 14, 149, 152
TfiI GAWTC 4 cut(s) 57, 227, 311, 428
Tru1I TTAA 2 cut(s) 408, 491
Tru9I TTAA 2 cut(s) 408, 491
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 4 cut(s) 193, 277, 332, 514
TseI GCWGC 3 cut(s) 5, 8, 200
Tsp45I GTSAC 4 cut(s) 193, 277, 332, 514
TspDTI ATGAA 7 cut(s) 60, 62, 143, 240, 324, 357, 510
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 120
XmiI GTMKAC 2 cut(s) 380, 496
XspI CTAG 2 cut(s) 38, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.