Rmu_sc0002497.1_g000001

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002497.1
OG0008378
Physical Location & Seq
Forward (+)
1 .. 1336
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
gtctcgtcgcagacctacagtctcgatgcgaatctctgccttcttcgtctctatcagtttgagccggagcgtatgagcactcaaattgttgctcgtattttggttaaggcactgatggcaatgcccgctcccgatttcagcctctgtctcttcctaattccagaaagagtgcaaatggaggagcagttcaagactctaattgtcctatctcactatctagagacaggaagattcagtcaattctgggatgatgcatctaagaatcgtcacatacttgaagctgtcccaggttttgagcaagcggtccaagcctatgcagttcatgttctttccttgacttaccaaaaggttcccagatctgtgcttgctgaggcaatcaaccttgaaggtatatccttggacaaattccttgagcatcatgttgccaatagcggttgggttcttgaaaaaggtcatggcaagggccaactcattgtcttacctcgaaatgaatttaaccatcctgagctgaagaaaagcactgacgatggaattccattagaccatgtggctcgaatcttccccatcctgggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

191

Amino Acids

21.65

Weight (kDa)

6.06

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 26 - 151 1e-16 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 128
AciI CCGC 3 cut(s) 126, 302, 432
AcsI RAATTY 3 cut(s) 404, 491, 531
AcuI CTGAAG 1 cut(s) 530
AfiI CCNNNNNNNGG 2 cut(s) 568, 569
AgsI TTSAA 4 cut(s) 190, 278, 386, 446
AhdI GACNNNNNGTC 1 cut(s) 18
AjnI CCWGG 2 cut(s) 286, 567
AloI GAACNNNNNNTCC 2 cut(s) 170, 202
AluBI AGCT 2 cut(s) 281, 508
AluI AGCT 2 cut(s) 281, 508
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 5 cut(s) 7, 26, 53, 152, 215
AlwNI CAGNNNCTG 1 cut(s) 144
AoxI GGCC 1 cut(s) 463
ApoI RAATTY 3 cut(s) 404, 491, 531
AspS9I GGNCC 2 cut(s) 304, 463
AvaII GGWCC 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 80
BbvCI CCTCAGC 1 cut(s) 369
BccI CCATC 4 cut(s) 109, 507, 521, 572
BciT130I CCWGG 2 cut(s) 288, 569
BcoDI GTCTC 5 cut(s) 7, 26, 53, 152, 215
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 16
BglII AGATCT 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 288, 569
Bme18I GGWCC 1 cut(s) 304
BmeRI GACNNNNNGTC 1 cut(s) 18
BmgT120I GGNCC 2 cut(s) 304, 463
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 288, 569
BmsI GCATC 4 cut(s) 16, 241, 263, 424
Bpu10I CCTNAGC 2 cut(s) 369, 504
BpuEI CTTGAG 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 30
BsaJI CCNNGG 3 cut(s) 286, 396, 568
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 568, 569
Bse3DI GCAATG 1 cut(s) 126
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 2 cut(s) 288, 569
BseDI CCNNGG 3 cut(s) 286, 396, 568
BseGI GGATG 3 cut(s) 253, 499, 564
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 568, 569
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 360, 495
BseRI GAGGAG 1 cut(s) 194
BshFI GGCC 1 cut(s) 465
BsiHKAI GWGCWC 1 cut(s) 80
BsiSI CCGG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 568, 569
BsmAI GTCTC 5 cut(s) 7, 26, 53, 152, 215
BsmBI CGTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 1 cut(s) 356
BspACI CCGC 3 cut(s) 126, 302, 432
BspANI GGCC 1 cut(s) 465
BspCNI CTCAG 2 cut(s) 361, 496
BspLI GGNNCC 1 cut(s) 351
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 126
BssECI CCNNGG 3 cut(s) 286, 396, 568
BssMI GATC 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 288, 569
Bst4CI ACNGT 1 cut(s) 20
Bst6I CTCTTC 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 126, 300, 366
BstDEI CTNAG 3 cut(s) 258, 369, 504
BstF5I GGATG 3 cut(s) 253, 499, 564
BstKTI GATC 1 cut(s) 359
BstMAI GTCTC 5 cut(s) 7, 26, 53, 152, 215
BstMBI GATC 1 cut(s) 356
BstMWI GCNNNNNNNGC 3 cut(s) 116, 125, 308
BstNI CCWGG 2 cut(s) 288, 569
BstSCI CCNGG 2 cut(s) 286, 567
BstSFI CTRYAG 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BsuRI GGCC 1 cut(s) 465
BtsCI GGATG 3 cut(s) 253, 499, 564
BtsIMutI CAGTG 2 cut(s) 110, 519
Cac8I GCNNGC 3 cut(s) 126, 300, 366
CaiI CAGNNNCTG 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 304, 463
CviAII CATG 4 cut(s) 323, 419, 455, 545
CviJI RGCY 7 cut(s) 64, 141, 281, 311, 465, 508, 551
CviKI_1 RGCY 7 cut(s) 64, 141, 281, 311, 465, 508, 551
DdeI CTNAG 3 cut(s) 258, 369, 504
DpnI GATC 1 cut(s) 358
DpnII GATC 1 cut(s) 356
DriI GACNNNNNGTC 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 155
Eam1105I GACNNNNNGTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 531
EcoRII CCWGG 2 cut(s) 286, 567
EcoT14I CCWWGG 1 cut(s) 396
EcoT22I ATGCAT 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 4 cut(s) 326, 422, 458, 548
FaiI YATR 8 cut(s) 74, 272, 315, 324, 392, 420, 456, 546
FaqI GGGAC 1 cut(s) 269
FatI CATG 4 cut(s) 322, 418, 454, 544
FauI CCCGC 1 cut(s) 133
FokI GGATG 3 cut(s) 260, 486, 551
FspBI CTAG 1 cut(s) 218
HaeIII GGCC 1 cut(s) 465
HapII CCGG 1 cut(s) 65
Hin1II CATG 4 cut(s) 326, 422, 458, 548
HinfI GANTC 5 cut(s) 31, 193, 231, 262, 555
HpaII CCGG 1 cut(s) 65
Hpy188III TCNNGA 7 cut(s) 23, 131, 161, 190, 218, 443, 503
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 50, 380
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4V TGCA 3 cut(s) 172, 254, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 125, 308
HpyF3I CTNAG 3 cut(s) 258, 369, 504
Hsp92II CATG 4 cut(s) 326, 422, 458, 548
Kzo9I GATC 1 cut(s) 356
LmnI GCTCC 3 cut(s) 67, 133, 181
LpnPI CCDG 9 cut(s) 78, 174, 210, 229, 273, 300, 367, 516, 554
LweI GCATC 4 cut(s) 16, 241, 263, 424
MaeI CTAG 1 cut(s) 218
MaeIII GTNAC 1 cut(s) 266
MalI GATC 1 cut(s) 358
MbiI CCGCTC 1 cut(s) 128
MboI GATC 1 cut(s) 356
MboII GAAGA 5 cut(s) 35, 142, 240, 523, 550
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 7 cut(s) 84, 156, 198, 239, 404, 491, 531
MlyI GAGTC 1 cut(s) 187
MnlI CCTC 4 cut(s) 152, 172, 364, 492
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 2 cut(s) 105, 495
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 288, 569
MvaI CCWGG 2 cut(s) 288, 569
MwoI GCNNNNNNNGC 3 cut(s) 116, 125, 308
NdeII GATC 1 cut(s) 356
NlaIII CATG 4 cut(s) 326, 422, 458, 548
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 266
NsiI ATGCAT 1 cut(s) 256
PfeI GAWTC 4 cut(s) 31, 231, 262, 555
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Psp6I CCWGG 2 cut(s) 286, 567
PspGI CCWGG 2 cut(s) 286, 567
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 2 cut(s) 304, 463
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 1 cut(s) 356
SaqAI TTAA 2 cut(s) 105, 495
Sau3AI GATC 1 cut(s) 356
Sau96I GGNCC 2 cut(s) 304, 463
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 2 cut(s) 288, 569
SduI GDGCHC 1 cut(s) 80
SetI ASST 9 cut(s) 17, 283, 292, 351, 384, 391, 454, 484, 510
SfaNI GCATC 4 cut(s) 16, 241, 263, 424
SfcI CTRYAG 1 cut(s) 16
SinI GGWCC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 7 cut(s) 84, 156, 198, 239, 404, 491, 531
SsiI CCGC 3 cut(s) 126, 302, 432
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 2 cut(s) 286, 567
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 1 cut(s) 20
TaqI TCGA 3 cut(s) 24, 484, 553
TasI AATT 7 cut(s) 84, 156, 198, 239, 404, 491, 531
TfiI GAWTC 4 cut(s) 31, 231, 262, 555
Tru1I TTAA 2 cut(s) 105, 495
Tru9I TTAA 2 cut(s) 105, 495
TscAI CASTG 2 cut(s) 117, 526
TseFI GTSAC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 2 cut(s) 311, 504
TspRI CASTG 2 cut(s) 117, 526
VpaK11BI GGWCC 1 cut(s) 304
XapI RAATTY 3 cut(s) 404, 491, 531
XbaI TCTAGA 1 cut(s) 217
XspI CTAG 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.