Rmu_sc0002539.1_g000113

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002539.1
OG0003606
Physical Location & Seq
Reverse (-)
462978 .. 465686
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2709 bp
atggtccaaagaaaggtacaaaaccagcttgcaaaacaagcagatcctaatgttaaattcgagaagcggttggtgaacctgaaaccatcttctcagtttcaagatggcaaaaatagaggaggagctgatttgaagataaagatgaagaagtcaagatcaaccaagctttcagatattgagagcttgagatcaccatcatcatcttcatcactacctttgagaaagagcatgtcccagcccggaaagccaccgcctctcaatgttcaaagcattgcagcagcagcagcagcagtccctctgaagcaggctttgaccaaaacaagtgatggctcagcaccaaactatatgaagtccaccagctgctctgatgcaaggaaggagcaatcacaggttagtgttcgaagttctccggttatttcagatagtaagaatcaaaaccgaagaaatttgagcaattcaaaacttagctctggctctaacagtaaactagctaggacttctggtctgaagctggtgagaactttaataaagtcccctagttttaagcctgcaagagcttcagctaagaaaacttccagggttgctctttgtgaagatgtgagtgttcagaaagcaacttgttcttcaacactgaaggactcaaagtttcctaagtatctgatgattagtcctggcggaacggaagctgagggaacttcaatgatgaaggtctgcccctatacttactgctctcttaatggacatcatcattcacctgtgactccactgaagtgtttcttgtcggcaagaagacggtctgtgaagaaccagaagatggtgaaattgcaagctctaagtcctcgtaaagcaaagccatctaatttgggaatgaaggaaaatgattccaagcaaatgttgtatgatgacaatgaaaaacctgctcaagaagaagtcgggatcgatttctttgttgaaatatttgctaaaagcaaggaagatgatgctcaaacaatttttgagaatatacctgaagcagaaaccatagatagctttcctgaagaggagcaaaaagaagatgtggctgatgaagaataccaaccttctttggatcaagaagaacctgccatgcgaagctgttcgagtgagagtgactccgatggactttcgagcgttgaagtagattatgccaattctgaagcaactgacatggagtgggaggaagggcaattttcccttgcagtgctgtatgatgatgatgaatctggttcaagtggtgggttttcatcaattcaagatggtgatatgcatgaagaacctgtgatcaagtctgatgccattgttattaactgcaatgacatcattcatgattactatgaagtactgcaagaacttttcacagagaaaactccatcttttgaaactcaactcaaaaatgatggctcaaaacagaatttcggcattgaagaatctgaaagattgagctatgaccaattttcttactctgaggattcatttgaagaagacagtgagctatcagagacagaaatagagatatcttcatcttcaatacaagagccaactgaggacctaacagcaactggggaggaagttcaagagaaaaccagagtttttgaagcagaggatcatgaaatggagtcccatattggagatgttgaaagccagtgcactgctgcttgtgagacagatgaagcttcagatagccaaccaaggaatgaatttgaagatgatggaacaaccatgtcaacaagtaaccaaatttctaatgccgctcaggatatctgcacaacatatgaagctgaaacaaatgcagagaaagagaattcagaaacagaccaaaatgctgtcacttctgtttctggaaatcaaatagaagaggcacagaaagttgaagacaccgaatcctcagcagatatccagatttctgattcagcaaaaactatggttgtacatgacgaagatgctagtaaggttggtgataatgcggacactgctgaagattgtaattcaggccaaggtattgctaatgaaaacacttttgttgagagtaaagataacctggctaatggggagtacacggataatcctaaagctgagaatcatagcttcacagagaaggatcaaagtgaatttaagaaggaaaagatctcaaattccagtgcaggtgaggatcagagtgatcaaaagctgaagcaaataggtgtagcagaaaacagtggtggagacattgatggaatggaagtagacaacaattcagggccagatgcagcagaaacttttcgtaaggccaatagtagcatcagtccagggatgaagagaaagttttcccatcaagaaagcaattctgacgaagaactcttaaacacaaaccggaaatggaaaatcaactgcaagagaactatcaaggatgaggaagaaaaatggaaatacaatcagagaaaacccaattacctgccgttggttcctggccctgaagcagagaaggttgacctgaggcatcagatgatggatgaaaagaaaaatgcagaggaatggatgctcgactttgcaatccaacaagctgtcaccaaacttgcgccagctaggaagaagaaggtggcattgcttgttgaagcatttgaaaaagtcatgccagtgccgcaatatgaaccccgtttgaagcattcttcagcagccttcagtcatgcaagacctatgcaagcttgtagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

902

Amino Acids

99.9

Weight (kDa)

4.78

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 778 - 877 4.5e-29 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2150
Acc36I ACCTGC 4 cut(s) 925, 1108, 2150, 2457
AccB7I CCANNNNNTGG 1 cut(s) 814
AccBSI CCGCTC 1 cut(s) 1769
AccI GTMKAC 1 cut(s) 2241
AciI CCGC 6 cut(s) 67, 251, 675, 1767, 1982, 2636
AclWI GGATC 6 cut(s) 38, 944, 1095, 1629, 2124, 2175
AcsI RAATTY 8 cut(s) 56, 445, 1429, 1715, 1755, 1819, 2126, 2149
AfaI GTAC 4 cut(s) 18, 1359, 1947, 2072
AfiI CCNNNNNNNGG 3 cut(s) 13, 814, 2455
AhdI GACNNNNNGTC 1 cut(s) 501
AjnI CCWGG 5 cut(s) 575, 670, 2055, 2302, 2461
AleI CACNNNNGTG 1 cut(s) 769
Alw21I GWGCWC 1 cut(s) 1667
Alw26I GTCTC 3 cut(s) 1511, 1673, 2214
Alw44I GTGCAC 1 cut(s) 1663
AlwI GGATC 6 cut(s) 38, 944, 1095, 1629, 2124, 2175
AlwNI CAGNNNCTG 1 cut(s) 1577
AoxI GGCC 4 cut(s) 2008, 2255, 2283, 2464
ApaLI GTGCAC 1 cut(s) 1663
ApeKI GCWGC 9 cut(s) 275, 278, 281, 284, 287, 360, 1670, 2264, 2669
ApoI RAATTY 8 cut(s) 56, 445, 1429, 1715, 1755, 1819, 2126, 2149
Asp700I GAANNNNTTC 4 cut(s) 773, 1114, 2274, 2320
AspLEI GCGC 1 cut(s) 2575
AspS9I GGNCC 4 cut(s) 4, 1564, 2255, 2465
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 9 cut(s) 85, 183, 526, 744, 829, 1289, 1985, 2174, 2554
AsuII TTCGAA 1 cut(s) 400
AvaII GGWCC 2 cut(s) 4, 1564
AxyI CCTNAGG 1 cut(s) 2489
BaeGI GKGCMC 1 cut(s) 1667
BbsI GAAGAC 3 cut(s) 796, 1506, 1896
Bbv12I GWGCWC 1 cut(s) 1667
BbvCI CCTCAGC 2 cut(s) 687, 1903
BbvI GCAGC 9 cut(s) 287, 290, 293, 296, 299, 347, 1657, 2276, 2681
BceAI ACGGC 1 cut(s) 2437
BciT130I CCWGG 5 cut(s) 577, 672, 2057, 2304, 2463
BclI TGATCA 2 cut(s) 1299, 2176
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 3 cut(s) 1511, 1673, 2214
BfaI CTAG 5 cut(s) 488, 492, 537, 1962, 2580
BfuAI ACCTGC 4 cut(s) 925, 1108, 2150, 2457
BglII AGATCT 1 cut(s) 2142
BlpI GCTNAGC 1 cut(s) 331
BmcAI AGTACT 1 cut(s) 1359
Bme1390I CCNGG 6 cut(s) 240, 577, 672, 2057, 2304, 2463
Bme18I GGWCC 2 cut(s) 4, 1564
BmeRI GACNNNNNGTC 1 cut(s) 501
BmgT120I GGNCC 4 cut(s) 4, 1564, 2255, 2465
BmiI GGNNCC 1 cut(s) 2460
BmrFI CCNGG 6 cut(s) 240, 577, 672, 2057, 2304, 2463
BmrI ACTGGG 1 cut(s) 1587
BmsI GCATC 8 cut(s) 358, 970, 1300, 1948, 2251, 2304, 2503, 2523
BmuI ACTGGG 1 cut(s) 1587
BpiI GAAGAC 3 cut(s) 796, 1506, 1896
BplI GAGNNNNNCTC 2 cut(s) 1115, 1147
Bpu10I CCTNAGC 3 cut(s) 687, 1770, 1903
Bpu1102I GCTNAGC 1 cut(s) 331
Bpu14I TTCGAA 1 cut(s) 400
BpuEI CTTGAG 2 cut(s) 205, 906
BpuMI CCSGG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 939
BsaBI GATNNNNATC 1 cut(s) 193
BsaJI CCNNGG 4 cut(s) 576, 1706, 2011, 2303
BsaWI WCCGGW 2 cut(s) 409, 2367
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 814, 2455
Bse1I ACTGG 4 cut(s) 1582, 1660, 2154, 2630
Bse21I CCTNAGG 1 cut(s) 2489
Bse3DI GCAATG 3 cut(s) 270, 1336, 2597
Bse8I GATNNNNATC 1 cut(s) 193
BseBI CCWGG 5 cut(s) 577, 672, 2057, 2304, 2463
BseCI ATCGAT 1 cut(s) 939
BseDI CCNNGG 4 cut(s) 576, 1706, 2011, 2303
BseGI GGATG 4 cut(s) 2313, 2410, 2512, 2538
BseJI GATNNNNATC 1 cut(s) 193
BseLI CCNNNNNNNGG 3 cut(s) 13, 814, 2455
BseMI GCAATG 3 cut(s) 270, 1336, 2597
BseMII CTCAG 9 cut(s) 107, 345, 678, 1473, 1551, 1784, 1917, 2082, 2480
BseNI ACTGG 4 cut(s) 1582, 1660, 2154, 2630
BseRI GAGGAG 3 cut(s) 132, 135, 1055
BseSI GKGCMC 1 cut(s) 1667
BseXI GCAGC 9 cut(s) 287, 290, 293, 296, 299, 347, 1657, 2276, 2681
BseYI CCCAGC 1 cut(s) 234
BsgI GTGCAG 2 cut(s) 1765, 2178
BshFI GGCC 4 cut(s) 2010, 2257, 2285, 2466
BshVI ATCGAT 1 cut(s) 939
BsiHKAI GWGCWC 1 cut(s) 1667
BsiSI CCGG 3 cut(s) 240, 410, 2368
BslFI GGGAC 4 cut(s) 217, 278, 517, 1621
BslI CCNNNNNNNGG 3 cut(s) 13, 814, 2455
BsmAI GTCTC 3 cut(s) 1511, 1673, 2214
BsmFI GGGAC 4 cut(s) 217, 278, 517, 1621
BsmI GAATGC 1 cut(s) 2659
BsnI GGCC 4 cut(s) 2010, 2257, 2285, 2466
Bsp119I TTCGAA 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 1667
Bsp1407I TGTACA 1 cut(s) 1945
Bsp1720I GCTNAGC 1 cut(s) 331
BspACI CCGC 6 cut(s) 67, 251, 675, 1767, 1982, 2636
BspANI GGCC 4 cut(s) 2010, 2257, 2285, 2466
BspCNI CTCAG 9 cut(s) 106, 344, 679, 1474, 1552, 1783, 1916, 2083, 2481
BspDI ATCGAT 1 cut(s) 939
BspHI TCATGA 2 cut(s) 1342, 1624
BspLI GGNNCC 1 cut(s) 2460
BspMI ACCTGC 4 cut(s) 925, 1108, 2150, 2457
BspPI GGATC 6 cut(s) 38, 944, 1095, 1629, 2124, 2175
BspT104I TTCGAA 1 cut(s) 400
BsrBI CCGCTC 1 cut(s) 1769
BsrDI GCAATG 3 cut(s) 270, 1336, 2597
BsrGI TGTACA 1 cut(s) 1945
BsrI ACTGG 4 cut(s) 1582, 1660, 2154, 2630
BssECI CCNNGG 4 cut(s) 576, 1706, 2011, 2303
BssT1I CCWWGG 2 cut(s) 1706, 2011
Bst2UI CCWGG 5 cut(s) 577, 672, 2057, 2304, 2463
Bst4CI ACNGT 4 cut(s) 482, 795, 1505, 2213
Bst6I CTCTTC 3 cut(s) 1032, 1866, 2306
BstAUI TGTACA 1 cut(s) 1945
BstBI TTCGAA 1 cut(s) 400
BstC8I GCNNGC 6 cut(s) 30, 306, 549, 828, 2577, 2697
BstF5I GGATG 4 cut(s) 2313, 2410, 2512, 2538
BstHHI GCGC 1 cut(s) 2575
BstMAI GTCTC 3 cut(s) 1511, 1673, 2214
BstMWI GCNNNNNNNGC 7 cut(s) 38, 244, 281, 284, 287, 1988, 2635
BstNI CCWGG 5 cut(s) 577, 672, 2057, 2304, 2463
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 6 cut(s) 238, 575, 670, 2055, 2302, 2461
BstSLI GKGCMC 1 cut(s) 1667
BstV1I GCAGC 9 cut(s) 287, 290, 293, 296, 299, 347, 1657, 2276, 2681
BstV2I GAAGAC 3 cut(s) 796, 1506, 1896
BstX2I RGATCY 2 cut(s) 43, 2142
BstYI RGATCY 2 cut(s) 43, 2142
Bsu15I ATCGAT 1 cut(s) 939
Bsu36I CCTNAGG 1 cut(s) 2489
BsuRI GGCC 4 cut(s) 2010, 2257, 2285, 2466
BsuTUI ATCGAT 1 cut(s) 939
BtsCI GGATG 4 cut(s) 2313, 2410, 2512, 2538
BtsI GCAGTG 3 cut(s) 1224, 1665, 1986
BveI ACCTGC 4 cut(s) 925, 1108, 2150, 2457
Cac8I GCNNGC 6 cut(s) 30, 306, 549, 828, 2577, 2697
CaiI CAGNNNCTG 1 cut(s) 1577
CciI TCATGA 2 cut(s) 1342, 1624
CfoI GCGC 1 cut(s) 2575
Cfr13I GGNCC 4 cut(s) 4, 1564, 2255, 2465
ClaI ATCGAT 1 cut(s) 939
Csp6I GTAC 4 cut(s) 17, 1358, 1946, 2071
CviQI GTAC 4 cut(s) 17, 1358, 1946, 2071
DriI GACNNNNNGTC 1 cut(s) 501
Eam1104I CTCTTC 3 cut(s) 1032, 1866, 2306
Eam1105I GACNNNNNGTC 1 cut(s) 501
EarI CTCTTC 3 cut(s) 1032, 1866, 2306
EciI GGCGGA 1 cut(s) 690
Eco130I CCWWGG 2 cut(s) 1706, 2011
Eco32I GATATC 3 cut(s) 1533, 1777, 1912
Eco47I GGWCC 2 cut(s) 4, 1564
Eco81I CCTNAGG 1 cut(s) 2489
EcoO109I RGGNCCY 1 cut(s) 1564
EcoRI GAATTC 1 cut(s) 1819
EcoRII CCWGG 5 cut(s) 575, 670, 2055, 2302, 2461
EcoRV GATATC 3 cut(s) 1533, 1777, 1912
EcoT14I CCWWGG 2 cut(s) 1706, 2011
EcoT22I ATGCAT 1 cut(s) 1287
ErhI CCWWGG 2 cut(s) 1706, 2011
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 4 cut(s) 217, 278, 517, 1621
FauNDI CATATG 1 cut(s) 1789
FbaI TGATCA 2 cut(s) 1299, 2176
FblI GTMKAC 1 cut(s) 2241
FokI GGATG 4 cut(s) 2320, 2417, 2519, 2545
FspBI CTAG 5 cut(s) 488, 492, 537, 1962, 2580
GlaI GCGC 1 cut(s) 2574
GsaI CCCAGC 1 cut(s) 238
HaeIII GGCC 4 cut(s) 2010, 2257, 2285, 2466
HapII CCGG 3 cut(s) 240, 410, 2368
HhaI GCGC 1 cut(s) 2575
Hin6I GCGC 1 cut(s) 2573
HinP1I GCGC 1 cut(s) 2573
HincII GTYRAC 2 cut(s) 1743, 2485
HindII GTYRAC 2 cut(s) 1743, 2485
HindIII AAGCTT 3 cut(s) 164, 1689, 2697
HpaII CCGG 3 cut(s) 240, 410, 2368
HphI GGTGA 9 cut(s) 85, 183, 526, 744, 829, 1289, 1985, 2174, 2554
Hpy166II GTNNAC 8 cut(s) 76, 354, 485, 1665, 1743, 2073, 2242, 2485
Hpy8I GTNNAC 8 cut(s) 76, 354, 485, 1665, 1743, 2073, 2242, 2485
HpyCH4III ACNGT 4 cut(s) 482, 795, 1505, 2213
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 244, 281, 284, 287, 1988, 2635
HspAI GCGC 1 cut(s) 2573
Ksp22I TGATCA 2 cut(s) 1299, 2176
LmnI GCTCC 3 cut(s) 122, 379, 1042
Lsp1109I GCAGC 9 cut(s) 287, 290, 293, 296, 299, 347, 1657, 2276, 2681
LweI GCATC 8 cut(s) 358, 970, 1300, 1948, 2251, 2304, 2503, 2523
MaeI CTAG 5 cut(s) 488, 492, 537, 1962, 2580
MaeIII GTNAC 5 cut(s) 757, 1127, 1748, 1843, 2560
MbiI CCGCTC 1 cut(s) 1769
MflI RGATCY 2 cut(s) 43, 2142
MhlI GDGCHC 1 cut(s) 1667
MlyI GAGTC 4 cut(s) 632, 754, 1124, 1643
MmeI TCCRAC 1 cut(s) 2575
Mph1103I ATGCAT 1 cut(s) 1287
MroXI GAANNNNTTC 4 cut(s) 773, 1114, 2274, 2320
MseI TTAA 7 cut(s) 54, 524, 543, 735, 1323, 2130, 2357
MslI CAYNNNNRTG 2 cut(s) 769, 2630
MspA1I CMGCKG 1 cut(s) 360
MspI CCGG 3 cut(s) 240, 410, 2368
MspR9I CCNGG 6 cut(s) 240, 577, 672, 2057, 2304, 2463
Mva1269I GAATGC 1 cut(s) 2659
MvaI CCWGG 5 cut(s) 577, 672, 2057, 2304, 2463
MwoI GCNNNNNNNGC 7 cut(s) 38, 244, 281, 284, 287, 1988, 2635
NciI CCSGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 1789
NlaIV GGNNCC 1 cut(s) 2460
NmuCI GTSAC 4 cut(s) 757, 1127, 1843, 2560
NsiI ATGCAT 1 cut(s) 1287
NspI RCATGY 1 cut(s) 232
NspV TTCGAA 1 cut(s) 400
OliI CACNNNNGTG 1 cut(s) 769
PagI TCATGA 2 cut(s) 1342, 1624
PaqCI CACCTGC 1 cut(s) 2150
PctI GAATGC 1 cut(s) 2659
PdmI GAANNNNTTC 4 cut(s) 773, 1114, 2274, 2320
PfeI GAWTC 8 cut(s) 430, 881, 1238, 1445, 1487, 1898, 1925, 2095
PflMI CCANNNNNTGG 1 cut(s) 814
PleI GAGTC 4 cut(s) 632, 754, 1124, 1642
PpsI GAGTC 4 cut(s) 632, 754, 1124, 1642
PpuMI RGGWCCY 1 cut(s) 1564
Psp5II RGGWCCY 1 cut(s) 1564
Psp6I CCWGG 5 cut(s) 575, 670, 2055, 2302, 2461
PspFI CCCAGC 1 cut(s) 234
PspGI CCWGG 5 cut(s) 575, 670, 2055, 2302, 2461
PspN4I GGNNCC 1 cut(s) 2460
PspPI GGNCC 4 cut(s) 4, 1564, 2255, 2465
PspPPI RGGWCCY 1 cut(s) 1564
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 2 cut(s) 43, 2142
PvuII CAGCTG 1 cut(s) 360
RsaI GTAC 4 cut(s) 18, 1359, 1947, 2072
RsaNI GTAC 4 cut(s) 17, 1358, 1946, 2071
RseI CAYNNNNRTG 2 cut(s) 769, 2630
SaqAI TTAA 7 cut(s) 54, 524, 543, 735, 1323, 2130, 2357
Sau96I GGNCC 4 cut(s) 4, 1564, 2255, 2465
ScaI AGTACT 1 cut(s) 1359
SchI GAGTC 4 cut(s) 632, 754, 1124, 1643
ScrFI CCNGG 6 cut(s) 240, 577, 672, 2057, 2304, 2463
SduI GDGCHC 1 cut(s) 1667
SfaNI GCATC 8 cut(s) 358, 970, 1300, 1948, 2251, 2304, 2503, 2523
SfuI TTCGAA 1 cut(s) 400
SinI GGWCC 2 cut(s) 4, 1564
SmiMI CAYNNNNRTG 2 cut(s) 769, 2630
SmlI CTYRAG 2 cut(s) 184, 921
SmoI CTYRAG 2 cut(s) 184, 921
SsiI CCGC 6 cut(s) 67, 251, 675, 1767, 1982, 2636
SspI AATATT 1 cut(s) 957
SspMI CTAG 5 cut(s) 488, 492, 537, 1962, 2580
StyD4I CCNGG 6 cut(s) 238, 575, 670, 2055, 2302, 2461
StyI CCWWGG 2 cut(s) 1706, 2011
TaaI ACNGT 4 cut(s) 482, 795, 1505, 2213
TaqI TCGA 6 cut(s) 60, 400, 939, 1118, 1145, 2538
TatI WGTACW 3 cut(s) 1357, 1945, 2070
TauI GCSGC 2 cut(s) 1769, 2638
TfiI GAWTC 8 cut(s) 430, 881, 1238, 1445, 1487, 1898, 1925, 2095
Tru1I TTAA 7 cut(s) 54, 524, 543, 735, 1323, 2130, 2357
Tru9I TTAA 7 cut(s) 54, 524, 543, 735, 1323, 2130, 2357
TseFI GTSAC 4 cut(s) 757, 1127, 1843, 2560
TseI GCWGC 9 cut(s) 275, 278, 281, 284, 287, 360, 1670, 2264, 2669
Tsp45I GTSAC 4 cut(s) 757, 1127, 1843, 2560
TspGWI ACGGA 2 cut(s) 695, 2090
Van91I CCANNNNNTGG 1 cut(s) 814
VneI GTGCAC 1 cut(s) 1663
VpaK11BI GGWCC 2 cut(s) 4, 1564
XapI RAATTY 8 cut(s) 56, 445, 1429, 1715, 1755, 1819, 2126, 2149
XceI RCATGY 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 2241
XmnI GAANNNNTTC 4 cut(s) 773, 1114, 2274, 2320
XspI CTAG 5 cut(s) 488, 492, 537, 1962, 2580
ZrmI AGTACT 1 cut(s) 1359
Zsp2I ATGCAT 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.