Rmu_sc0003517.1_g000017

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003517.1
OG0004967
Physical Location & Seq
Forward (+)
72521 .. 74989
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
atgatggaactgaacgattcgagtagcgacgaagtccgggacgccggagctgagccgacgaggggggccggactcgggatcgatctcaatgagaggatcccttcccctccagaaaccctaccggattcactagacgtggtccggtattatcaagacaatccatctccgccgccgggaggcccggccggagttcccggaggcggtcgcggctctgcctgcgcctcctgtgggaagcccgaggtccgcggacacgtggtggtctgcgacggctgcgagcgagggttccacctcacctgcgccggaatgcgcggccgccaggctgtcaatttggacgagtgggtctgcggcgagtgccagtgcggcggggttaagagcaagcggtggccgctcggtgttaagtccaagcagattttggatatcaacgcgtcgccgccgagcgacggcgacggcgacgctgacgtggccgaggaactgattgagttgagtgcggaagtttccgcccttggttttgtgaataatggagaattgtcagaagctggatcaaaaggaattggatgcatcatgtcgcagcacaatagtgagggatttccagtgcagtttgaagatttctttgttctttctttaggaaaaacggtggtgattctggaccagtttttaaggtcagaagatgttcgtgctctgccttacccattcccagaggttcgactattctgtataggccagcacttggtgatttttgcactcaaagtgatcaagacagtcatgacacgtacggaaatgatggaagtatacagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0001763 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005911 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008080 GO:0008150 GO:0008152 GO:0008327 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009648 GO:0009653 GO:0009791 GO:0009889 GO:0009987 GO:0010016 GO:0010216 GO:0010223 GO:0010228 GO:0010346 GO:0010468 GO:0010556 GO:0016043 GO:0016407 GO:0016410 GO:0016567 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022414 GO:0030054 GO:0031323 GO:0031326 GO:0031935 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034212 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048367 GO:0048573 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051252 GO:0051276 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0061630 GO:0061659 GO:0061733 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0097159 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905393 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain DDT FYRC MBD PHD WHIM1 WSD

Protein Analysis

265

Amino Acids

28.41

Weight (kDa)

4.73

Isoelectric Point (pI)

48.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 73 - 118 8.5e-09 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 302
AasI GACNNNNNNGTC 1 cut(s) 338
Acc36I ACCTGC 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 727
AccBSI CCGCTC 1 cut(s) 388
AccI GTMKAC 1 cut(s) 789
AccII CGCG 4 cut(s) 207, 246, 309, 425
AclWI GGATC 4 cut(s) 86, 91, 104, 547
AcoI YGGCCR 4 cut(s) 183, 310, 383, 462
AcvI CACGTG 1 cut(s) 253
AcyI GRCGYC 1 cut(s) 42
AdeI CACNNNGTG 2 cut(s) 256, 730
AfaI GTAC 1 cut(s) 772
AfiI CCNNNNNNNGG 8 cut(s) 62, 75, 173, 176, 200, 228, 440, 727
AflIII ACRYGT 3 cut(s) 250, 423, 767
AgsI TTSAA 1 cut(s) 602
AjiI CACGTC 2 cut(s) 136, 460
AjnI CCWGG 1 cut(s) 315
AleI CACNNNNGTG 1 cut(s) 576
AluBI AGCT 2 cut(s) 50, 536
AluI AGCT 2 cut(s) 50, 536
Alw21I GWGCWC 1 cut(s) 679
AlwI GGATC 4 cut(s) 86, 91, 104, 547
AlwNI CAGNNNCTG 1 cut(s) 536
Ama87I CYCGRG 2 cut(s) 74, 236
AoxI GGCC 7 cut(s) 66, 178, 183, 310, 383, 462, 718
ApeKI GCWGC 2 cut(s) 270, 568
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 3 cut(s) 221, 299, 309
AspS9I GGNCC 5 cut(s) 66, 139, 179, 241, 646
AsuC2I CCSGG 4 cut(s) 38, 174, 182, 195
AsuHPI GGTGA 3 cut(s) 283, 649, 742
AvaI CYCGRG 2 cut(s) 74, 236
AvaII GGWCC 3 cut(s) 139, 241, 646
BamHI GGATCC 1 cut(s) 96
BbrPI CACGTG 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 2 cut(s) 257, 580
BccI CCATC 2 cut(s) 169, 775
BceAI ACGGC 3 cut(s) 283, 457, 463
BciT130I CCWGG 1 cut(s) 317
BclI TGATCA 1 cut(s) 750
BcnI CCSGG 4 cut(s) 38, 174, 182, 195
BfaI CTAG 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 360
BlpI GCTNAGC 1 cut(s) 51
Bme1390I CCNGG 5 cut(s) 38, 174, 182, 195, 317
Bme18I GGWCC 3 cut(s) 139, 241, 646
BmeT110I CYCGRG 2 cut(s) 74, 236
BmgBI CACGTC 2 cut(s) 136, 460
BmgT120I GGNCC 5 cut(s) 66, 139, 179, 241, 646
BmiI GGNNCC 3 cut(s) 67, 98, 284
BmrFI CCNGG 5 cut(s) 38, 174, 182, 195, 317
BmsI GCATC 2 cut(s) 545, 567
BpmI CTGGAG 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 51
BpuMI CCSGG 4 cut(s) 38, 174, 182, 195
Bsa29I ATCGAT 1 cut(s) 81
BsaAI YACGTR 2 cut(s) 253, 770
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 237, 244, 465, 502
BsaWI WCCGGW 2 cut(s) 121, 141
Bsc4I CCNNNNNNNGG 8 cut(s) 62, 75, 173, 176, 200, 228, 440, 727
Bse1I ACTGG 3 cut(s) 355, 590, 649
BseBI CCWGG 1 cut(s) 317
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 4 cut(s) 237, 244, 465, 502
BseGI GGATG 1 cut(s) 560
BseLI CCNNNNNNNGG 8 cut(s) 62, 75, 173, 176, 200, 228, 440, 727
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 3 cut(s) 355, 590, 649
BseX3I CGGCCG 2 cut(s) 183, 310
BseXI GCAGC 2 cut(s) 257, 580
BsgI GTGCAG 1 cut(s) 614
Bsh1236I CGCG 4 cut(s) 207, 246, 309, 425
Bsh1285I CGRYCG 3 cut(s) 186, 205, 313
BshFI GGCC 7 cut(s) 68, 180, 185, 312, 385, 464, 720
BshVI ATCGAT 1 cut(s) 81
BsiEI CGRYCG 3 cut(s) 186, 205, 313
BsiHKAI GWGCWC 1 cut(s) 679
BsiHKCI CYCGRG 2 cut(s) 74, 236
BsiWI CGTACG 1 cut(s) 770
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 8 cut(s) 62, 75, 173, 176, 200, 228, 440, 727
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 309
BsnI GGCC 7 cut(s) 68, 180, 185, 312, 385, 464, 720
BsoBI CYCGRG 2 cut(s) 74, 236
Bsp1286I GDGCHC 1 cut(s) 679
Bsp143I GATC 5 cut(s) 78, 82, 96, 539, 750
Bsp1720I GCTNAGC 1 cut(s) 51
BspANI GGCC 7 cut(s) 68, 180, 185, 312, 385, 464, 720
BspCNI CTCAG 1 cut(s) 43
BspDI ATCGAT 1 cut(s) 81
BspFNI CGCG 4 cut(s) 207, 246, 309, 425
BspHI TCATGA 1 cut(s) 762
BspLI GGNNCC 3 cut(s) 67, 98, 284
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 4 cut(s) 86, 91, 104, 547
BsrBI CCGCTC 1 cut(s) 388
BsrI ACTGG 3 cut(s) 355, 590, 649
BssECI CCNNGG 4 cut(s) 237, 244, 465, 502
BssMI GATC 5 cut(s) 78, 82, 96, 539, 750
BssNAI GTATAC 1 cut(s) 790
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 502
Bst1107I GTATAC 1 cut(s) 790
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 2 cut(s) 634, 760
BstACI GRCGYC 1 cut(s) 42
BstBAI YACGTR 2 cut(s) 253, 770
BstC8I GCNNGC 4 cut(s) 217, 275, 377, 722
BstDEI CTNAG 1 cut(s) 51
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 560
BstFNI CGCG 4 cut(s) 207, 246, 309, 425
BstHHI GCGC 3 cut(s) 221, 299, 309
BstKTI GATC 5 cut(s) 81, 85, 99, 542, 753
BstMBI GATC 5 cut(s) 78, 82, 96, 539, 750
BstMCI CGRYCG 3 cut(s) 186, 205, 313
BstMWI GCNNNNNNNGC 7 cut(s) 207, 216, 270, 351, 360, 385, 461
BstNI CCWGG 1 cut(s) 317
BstSCI CCNGG 5 cut(s) 36, 172, 180, 193, 315
BstUI CGCG 4 cut(s) 207, 246, 309, 425
BstV1I GCAGC 2 cut(s) 257, 580
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
BstZ17I GTATAC 1 cut(s) 790
BstZI CGGCCG 2 cut(s) 183, 310
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 7 cut(s) 68, 180, 185, 312, 385, 464, 720
BsuTUI ATCGAT 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 244
BtrI CACGTC 2 cut(s) 136, 460
BtsCI GGATG 1 cut(s) 560
BtsIMutI CAGTG 2 cut(s) 362, 597
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 4 cut(s) 217, 275, 377, 722
CaiI CAGNNNCTG 1 cut(s) 536
CciI TCATGA 1 cut(s) 762
CciNI GCGGCCGC 1 cut(s) 310
CfoI GCGC 3 cut(s) 221, 299, 309
Cfr13I GGNCC 5 cut(s) 66, 139, 179, 241, 646
Cfr42I CCGCGG 1 cut(s) 247
ClaI ATCGAT 1 cut(s) 81
CseI GACGC 3 cut(s) 50, 414, 461
Csp6I GTAC 1 cut(s) 771
CviAII CATG 2 cut(s) 562, 763
CviQI GTAC 1 cut(s) 771
DdeI CTNAG 1 cut(s) 51
DpnI GATC 5 cut(s) 80, 84, 98, 541, 752
DpnII GATC 5 cut(s) 78, 82, 96, 539, 750
DraIII CACNNNGTG 2 cut(s) 256, 730
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 4 cut(s) 183, 310, 383, 462
EagI CGGCCG 2 cut(s) 183, 310
EciI GGCGGA 2 cut(s) 156, 487
EclXI CGGCCG 2 cut(s) 183, 310
Eco130I CCWWGG 1 cut(s) 502
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 3 cut(s) 139, 241, 646
Eco52I CGGCCG 2 cut(s) 183, 310
Eco72I CACGTG 1 cut(s) 253
Eco88I CYCGRG 2 cut(s) 74, 236
EcoRII CCWGG 1 cut(s) 315
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 502
EcoT22I ATGCAT 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 502
FaeI CATG 2 cut(s) 565, 766
FaiI YATR 4 cut(s) 563, 716, 764, 790
FaqI GGGAC 1 cut(s) 53
FatI CATG 2 cut(s) 561, 762
FauI CCCGC 1 cut(s) 356
FbaI TGATCA 1 cut(s) 750
FblI GTMKAC 1 cut(s) 789
FokI GGATG 1 cut(s) 567
FspBI CTAG 1 cut(s) 131
GlaI GCGC 3 cut(s) 220, 298, 308
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 7 cut(s) 68, 180, 185, 312, 385, 464, 720
HgaI GACGC 3 cut(s) 50, 414, 461
HhaI GCGC 3 cut(s) 221, 299, 309
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 2 cut(s) 565, 766
Hin6I GCGC 3 cut(s) 219, 297, 307
HinP1I GCGC 3 cut(s) 219, 297, 307
HinfI GANTC 4 cut(s) 17, 72, 125, 640
HphI GGTGA 3 cut(s) 283, 649, 742
Hpy166II GTNNAC 1 cut(s) 790
Hpy188I TCNGA 2 cut(s) 532, 664
Hpy188III TCNNGA 6 cut(s) 76, 110, 152, 644, 754, 763
Hpy8I GTNNAC 1 cut(s) 790
Hpy99I CGWCG 7 cut(s) 32, 61, 269, 430, 443, 449, 455
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 2 cut(s) 634, 760
HpyCH4IV ACGT 4 cut(s) 135, 252, 459, 769
HpyCH4V TGCA 3 cut(s) 558, 595, 740
HpyF10VI GCNNNNNNNGC 7 cut(s) 207, 216, 270, 351, 360, 385, 461
HpyF3I CTNAG 1 cut(s) 51
HpySE526I ACGT 4 cut(s) 135, 252, 459, 769
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 2 cut(s) 565, 766
HspAI GCGC 3 cut(s) 219, 297, 307
Ksp22I TGATCA 1 cut(s) 750
KspI CCGCGG 1 cut(s) 247
Kzo9I GATC 5 cut(s) 78, 82, 96, 539, 750
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 2 cut(s) 257, 580
LweI GCATC 2 cut(s) 545, 567
MaeI CTAG 1 cut(s) 131
MaeII ACGT 4 cut(s) 135, 252, 459, 769
MalI GATC 5 cut(s) 80, 84, 98, 541, 752
MbiI CCGCTC 1 cut(s) 388
MboI GATC 5 cut(s) 78, 82, 96, 539, 750
MboII GAAGA 2 cut(s) 614, 677
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 3 cut(s) 325, 524, 549
MluI ACGCGT 1 cut(s) 423
MlyI GAGTC 1 cut(s) 66
Mph1103I ATGCAT 1 cut(s) 560
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 3 cut(s) 369, 396, 656
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 246
MspR9I CCNGG 5 cut(s) 38, 174, 182, 195, 317
Mva1269I GAATGC 1 cut(s) 309
MvaI CCWGG 1 cut(s) 317
MvnI CGCG 4 cut(s) 207, 246, 309, 425
MwoI GCNNNNNNNGC 7 cut(s) 207, 216, 270, 351, 360, 385, 461
NciI CCSGG 4 cut(s) 38, 174, 182, 195
NdeII GATC 5 cut(s) 78, 82, 96, 539, 750
NlaIII CATG 2 cut(s) 565, 766
NlaIV GGNNCC 3 cut(s) 67, 98, 284
NmeAIII GCCGAG 2 cut(s) 459, 490
NotI GCGGCCGC 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 560
OliI CACNNNNGTG 1 cut(s) 576
PagI TCATGA 1 cut(s) 762
PaqCI CACCTGC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 3 cut(s) 17, 125, 640
Pfl23II CGTACG 1 cut(s) 770
PflFI GACNNNGTC 2 cut(s) 32, 137
PflMI CCANNNNNTGG 1 cut(s) 727
PfoI TCCNGGA 2 cut(s) 36, 193
PleI GAGTC 1 cut(s) 66
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
PpsI GAGTC 1 cut(s) 66
Ppu21I YACGTR 2 cut(s) 253, 770
Psp6I CCWGG 1 cut(s) 315
PspCI CACGTG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 315
PspLI CGTACG 1 cut(s) 770
PspN4I GGNNCC 3 cut(s) 67, 98, 284
PspPI GGNCC 5 cut(s) 66, 139, 179, 241, 646
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 1 cut(s) 96
PsyI GACNNNGTC 2 cut(s) 32, 137
RsaI GTAC 1 cut(s) 772
RsaNI GTAC 1 cut(s) 771
RseI CAYNNNNRTG 1 cut(s) 576
SacII CCGCGG 1 cut(s) 247
SaqAI TTAA 3 cut(s) 369, 396, 656
Sau3AI GATC 5 cut(s) 78, 82, 96, 539, 750
Sau96I GGNCC 5 cut(s) 66, 139, 179, 241, 646
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 5 cut(s) 38, 174, 182, 195, 317
SduI GDGCHC 1 cut(s) 679
SfaNI GCATC 2 cut(s) 545, 567
Sfr303I CCGCGG 1 cut(s) 247
SgrBI CCGCGG 1 cut(s) 247
SinI GGWCC 3 cut(s) 139, 241, 646
SmiMI CAYNNNNRTG 1 cut(s) 576
Sse9I AATT 3 cut(s) 325, 524, 549
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 5 cut(s) 36, 172, 180, 193, 315
StyI CCWWGG 1 cut(s) 502
TaaI ACNGT 2 cut(s) 634, 760
TaiI ACGT 4 cut(s) 138, 255, 462, 772
TaqI TCGA 3 cut(s) 20, 81, 703
TasI AATT 3 cut(s) 325, 524, 549
TauI GCSGC 8 cut(s) 172, 210, 312, 315, 348, 363, 388, 433
TfiI GAWTC 3 cut(s) 17, 125, 640
Tru1I TTAA 3 cut(s) 369, 396, 656
Tru9I TTAA 3 cut(s) 369, 396, 656
TscAI CASTG 2 cut(s) 362, 597
TseI GCWGC 2 cut(s) 270, 568
TspGWI ACGGA 1 cut(s) 788
TspRI CASTG 2 cut(s) 362, 597
Tth111I GACNNNGTC 2 cut(s) 32, 137
Van91I CCANNNNNTGG 1 cut(s) 727
VpaK11BI GGWCC 3 cut(s) 139, 241, 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 789
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.