Rmu_sc0003941.1_g000023

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003941.1
OG0002090
Physical Location & Seq
Forward (+)
118566 .. 123465
4900 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1722 bp
atggacgagaattcagcactgatcgaatcgattctgagagaggaagacgaggcggaggcagacaaatatgtaaagaagatgagcagcactgcgccagtccaaaacggcgacgcttacgcctggcaaactgtttcttatcacaagagaaaccgacgactttctcagccaattccgcctgattctgtcggcgatcgctccaacggcgacgtcttccgctcgatcgagctgcattccgaggaccgccggaggagaattcgacaggctcaaacggattcccttcccgctgtccgatccgagctgcattccgacgacgatagcgatgccgaggtggccgctgccgcggaaaatggcgagccgaagaaggccaaagtgaagaagccgaagaagccaaagctgactgtggccgaggcggcttccaagatcgacgcggctcatctcggtgcttttctcgccgagataacggaggtgtatgaatcccaacaagacatactgcttttgcgtctggctgattatttcggtcgagcatttgcgtccgtcaccgccgctcagtttccctggttgaagatattcaaggaatccactgtctcaaagatggttgacatccctctttctcatatatctgaaaatgtttataagacatcagctgactggatcggccagcgctccactgaagcccttgggtccttcgtgttgtggtcactagattgcattcttgctgaccttgcaagtcatcagggagcagcaaagggatccaaaaaggtggtccagcaaccgtcaaaatgtcaggtaacttttggttgtagcataattttatcatctccaaaagctcggcctattctattaaatggtgctgttaggaaaggggagcacatagtgccaccatcagcccttgatctcttgatgagagtttcttttccttcaccttcagcgcgagttaaggcaactgagagatttgaagctgtctatccaaccttgaaagagattgcccttgctgggtccccaggaagcaaaacagtgaggcaggttgcacagaagacactgaattattcagttaaagctgttaaagaaggcatccccgatctctctaaggaggcaaccgctatatttatctggtgtttgacacaaagtcccgaatgttacaagcaatgggacatgctttatctggacaatcttgatgcaagtgttattgtgctgaaaaagttatctgacgagtggaaggatcactccgttaaacatgcctctcttgaccccttgagggaaaccttgaagagtttcagggagaagaatgaaaaagcattgccaaacggagatgatttggtgcgccattcgttgttgaaggatgcagataaatattgcaagctgattttaggacgccatgtctccactccgtcgattaacaccaccagcaagctctctcctccttttcctcctccaccactactagacatagaagacaacgatgaggaagaagcagtcaagtacaatgaaccgaaaaagaagttagagctgcccaaagtcaagttctgccaagagggtaagattttcgtggggaatttacccaactgggtcaagaataacaaggtcttcaagtttttccgacaatcccatcttctcatcttcttcctcctcaaaccccatctacgcctcgccttcaatcccagccaatttgagtccaagtctccgatgccgaaccactgccgccatagcactcaccacccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

573

Amino Acids

64.19

Weight (kDa)

8.87

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 284 - 457 4.6e-12 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 1012
AccBSI CCGCTC 2 cut(s) 216, 545
AccII CGCG 3 cut(s) 341, 428, 931
AclWI GGATC 5 cut(s) 285, 659, 744, 757, 1227
AcoI YGGCCR 3 cut(s) 330, 402, 655
AcsI RAATTY 3 cut(s) 10, 252, 1552
AcuI CTGAAG 2 cut(s) 690, 909
AcyI GRCGYC 2 cut(s) 207, 1372
AdeI CACNNNGTG 1 cut(s) 874
AfaI GTAC 1 cut(s) 1481
AfeI AGCGCT 1 cut(s) 662
AfiI CCNNNNNNNGG 2 cut(s) 993, 1255
AgsI TTSAA 8 cut(s) 562, 571, 956, 976, 1267, 1336, 1588, 1654
AhdI GACNNNNNGTC 2 cut(s) 1125, 1376
AjnI CCWGG 3 cut(s) 119, 554, 1000
AjuI GAANNNNNNNTTGG 2 cut(s) 1505, 1537
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 3 cut(s) 589, 1384, 1683
AlwI GGATC 5 cut(s) 285, 659, 744, 757, 1227
Aor51HI AGCGCT 1 cut(s) 662
AoxI GGCC 5 cut(s) 330, 363, 402, 655, 830
ApeKI GCWGC 6 cut(s) 84, 226, 298, 335, 740, 1507
ApoI RAATTY 3 cut(s) 10, 252, 1552
ArsI GACNNNNNNTTYG 2 cut(s) 747, 779
AsiSI GCGATCGC 1 cut(s) 193
Asp700I GAANNNNTTC 3 cut(s) 30, 566, 1271
AspLEI GCGC 4 cut(s) 94, 663, 931, 1323
AspS9I GGNCC 4 cut(s) 238, 681, 763, 996
AsuHPI GGTGA 3 cut(s) 529, 912, 1703
AvaII GGWCC 4 cut(s) 238, 681, 763, 996
BamHI GGATCC 1 cut(s) 749
BbsI GAAGAC 5 cut(s) 51, 202, 1040, 1458, 1576
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 6 cut(s) 96, 213, 285, 322, 752, 1494
BccI CCATC 4 cut(s) 586, 889, 1614, 1644
BceAI ACGGC 2 cut(s) 121, 217
BcgI CGANNNNNNTGC 4 cut(s) 208, 242, 302, 336
BciT130I CCWGG 3 cut(s) 121, 556, 1002
BcoDI GTCTC 3 cut(s) 589, 1384, 1683
BfaI CTAG 2 cut(s) 701, 1442
BfoI RGCGCY 1 cut(s) 664
BfuAI ACCTGC 1 cut(s) 1012
BglI GCCNNNNNGGC 2 cut(s) 329, 410
Bme1390I CCNGG 3 cut(s) 121, 556, 1002
Bme18I GGWCC 4 cut(s) 238, 681, 763, 996
BmeRI GACNNNNNGTC 2 cut(s) 1125, 1376
BmgT120I GGNCC 4 cut(s) 238, 681, 763, 996
BmiI GGNNCC 4 cut(s) 682, 751, 997, 998
BmrFI CCNGG 3 cut(s) 121, 556, 1002
BmrI ACTGGG 1 cut(s) 1573
BmsI GCATC 5 cut(s) 310, 1080, 1165, 1330, 1674
BmuI ACTGGG 1 cut(s) 1573
BpiI GAAGAC 5 cut(s) 51, 202, 1040, 1458, 1576
BpuEI CTTGAG 1 cut(s) 1273
Bsa29I ATCGAT 1 cut(s) 29
BsaHI GRCGYC 2 cut(s) 207, 1372
BsaJI CCNNGG 7 cut(s) 234, 324, 339, 405, 554, 676, 1000
Bsc4I CCNNNNNNNGG 2 cut(s) 993, 1255
Bse1I ACTGG 3 cut(s) 95, 653, 1568
Bse3DI GCAATG 2 cut(s) 1151, 1295
BseBI CCWGG 3 cut(s) 121, 556, 1002
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 7 cut(s) 234, 324, 339, 405, 554, 676, 1000
BseGI GGATG 3 cut(s) 600, 1071, 1345
BseLI CCNNNNNNNGG 2 cut(s) 993, 1255
BseMI GCAATG 2 cut(s) 1151, 1295
BseMII CTCAG 4 cut(s) 26, 176, 560, 936
BseNI ACTGG 3 cut(s) 95, 653, 1568
BseRI GAGGAG 4 cut(s) 262, 1407, 1419, 1616
BseXI GCAGC 6 cut(s) 96, 213, 285, 322, 752, 1494
BseYI CCCAGC 2 cut(s) 992, 1658
Bsh1236I CGCG 3 cut(s) 341, 428, 931
Bsh1285I CGRYCG 3 cut(s) 193, 222, 520
BshFI GGCC 5 cut(s) 332, 365, 404, 657, 832
BshVI ATCGAT 1 cut(s) 29
BsiEI CGRYCG 3 cut(s) 193, 222, 520
BsiHKAI GWGCWC 1 cut(s) 870
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 3 cut(s) 982, 1113, 1163
BslI CCNNNNNNNGG 2 cut(s) 993, 1255
BsmAI GTCTC 3 cut(s) 589, 1384, 1683
BsmFI GGGAC 3 cut(s) 982, 1113, 1163
BsmI GAATGC 3 cut(s) 229, 301, 708
BsnI GGCC 5 cut(s) 332, 365, 404, 657, 832
Bsp1286I GDGCHC 1 cut(s) 870
BspANI GGCC 5 cut(s) 332, 365, 404, 657, 832
BspCNI CTCAG 4 cut(s) 27, 175, 559, 937
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 3 cut(s) 341, 428, 931
BspLI GGNNCC 4 cut(s) 682, 751, 997, 998
BspMI ACCTGC 1 cut(s) 1012
BspPI GGATC 5 cut(s) 285, 659, 744, 757, 1227
BsrBI CCGCTC 2 cut(s) 216, 545
BsrDI GCAATG 2 cut(s) 1151, 1295
BsrI ACTGG 3 cut(s) 95, 653, 1568
BssECI CCNNGG 7 cut(s) 234, 324, 339, 405, 554, 676, 1000
BssNI GRCGYC 2 cut(s) 207, 1372
BssT1I CCWWGG 1 cut(s) 676
Bst2UI CCWGG 3 cut(s) 121, 556, 1002
Bst4CI ACNGT 5 cut(s) 130, 400, 583, 774, 1015
Bst6I CTCTTC 1 cut(s) 1262
BstACI GRCGYC 2 cut(s) 207, 1372
BstAPI GCANNNNNTGC 1 cut(s) 874
BstC8I GCNNGC 4 cut(s) 353, 659, 1358, 1409
BstDEI CTNAG 5 cut(s) 35, 162, 546, 945, 1086
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 600, 1071, 1345
BstFNI CGCG 3 cut(s) 341, 428, 931
BstH2I RGCGCY 1 cut(s) 664
BstHHI GCGC 4 cut(s) 94, 663, 931, 1323
BstMAI GTCTC 3 cut(s) 589, 1384, 1683
BstMCI CGRYCG 3 cut(s) 193, 222, 520
BstNI CCWGG 3 cut(s) 121, 556, 1002
BstNSI RCATGY 2 cut(s) 1156, 1238
BstSCI CCNGG 3 cut(s) 119, 554, 1000
BstUI CGCG 3 cut(s) 341, 428, 931
BstV1I GCAGC 6 cut(s) 96, 213, 285, 322, 752, 1494
BstV2I GAAGAC 5 cut(s) 51, 202, 1040, 1458, 1576
BstX2I RGATCY 1 cut(s) 749
BstXI CCANNNNNNTGG 1 cut(s) 760
BstYI RGATCY 1 cut(s) 749
Bsu15I ATCGAT 1 cut(s) 29
BsuRI GGCC 5 cut(s) 332, 365, 404, 657, 832
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 3 cut(s) 600, 1071, 1345
BtsI GCAGTG 2 cut(s) 87, 1693
BtsIMutI CAGTG 7 cut(s) 17, 87, 579, 666, 1020, 1037, 1693
BveI ACCTGC 1 cut(s) 1012
Cac8I GCNNGC 4 cut(s) 353, 659, 1358, 1409
CfoI GCGC 4 cut(s) 94, 663, 931, 1323
Cfr13I GGNCC 4 cut(s) 238, 681, 763, 996
Cfr42I CCGCGG 1 cut(s) 342
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 5 cut(s) 119, 434, 488, 519, 1380
Csp6I GTAC 1 cut(s) 1480
CviAII CATG 3 cut(s) 1153, 1235, 1376
CviQI GTAC 1 cut(s) 1480
DdeI CTNAG 5 cut(s) 35, 162, 546, 945, 1086
DraIII CACNNNGTG 1 cut(s) 874
DriI GACNNNNNGTC 2 cut(s) 1125, 1376
EaeI YGGCCR 3 cut(s) 330, 402, 655
Eam1104I CTCTTC 1 cut(s) 1262
Eam1105I GACNNNNNGTC 2 cut(s) 1125, 1376
EarI CTCTTC 1 cut(s) 1262
EciI GGCGGA 2 cut(s) 68, 162
Eco130I CCWWGG 1 cut(s) 676
Eco47I GGWCC 4 cut(s) 238, 681, 763, 996
Eco47III AGCGCT 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 690, 909
EcoO109I RGGNCCY 2 cut(s) 681, 996
EcoRI GAATTC 2 cut(s) 10, 252
EcoRII CCWGG 3 cut(s) 119, 554, 1000
EcoT14I CCWWGG 1 cut(s) 676
ErhI CCWWGG 1 cut(s) 676
FaeI CATG 3 cut(s) 1156, 1238, 1379
FaqI GGGAC 3 cut(s) 982, 1113, 1163
FatI CATG 3 cut(s) 1152, 1234, 1375
FauI CCCGC 1 cut(s) 289
FokI GGATG 3 cut(s) 587, 1058, 1352
FspBI CTAG 2 cut(s) 701, 1442
GlaI GCGC 4 cut(s) 93, 662, 930, 1322
GsaI CCCAGC 2 cut(s) 996, 1662
HaeII RGCGCY 1 cut(s) 664
HaeIII GGCC 5 cut(s) 332, 365, 404, 657, 832
HapII CCGG 1 cut(s) 244
HgaI GACGC 5 cut(s) 119, 434, 488, 519, 1380
HhaI GCGC 4 cut(s) 94, 663, 931, 1323
Hin1I GRCGYC 2 cut(s) 207, 1372
Hin1II CATG 3 cut(s) 1156, 1238, 1379
Hin6I GCGC 4 cut(s) 92, 661, 929, 1321
HinP1I GCGC 4 cut(s) 92, 661, 929, 1321
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 7 cut(s) 26, 31, 179, 272, 473, 575, 1670
HpaII CCGG 1 cut(s) 244
HphI GGTGA 3 cut(s) 529, 912, 1703
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 9 cut(s) 36, 235, 290, 295, 307, 622, 1207, 1598, 1683
Hpy188III TCNNGA 6 cut(s) 898, 1130, 1163, 1172, 1244, 1570
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 7 cut(s) 113, 156, 209, 311, 314, 428, 1393
HpyAV CCTTC 9 cut(s) 287, 355, 694, 927, 933, 1061, 1210, 1330, 1660
HpyCH4III ACNGT 5 cut(s) 130, 400, 583, 774, 1015
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 8 cut(s) 229, 301, 708, 725, 1028, 1178, 1343, 1356
HpyF3I CTNAG 5 cut(s) 35, 162, 546, 945, 1086
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 2 cut(s) 207, 1372
Hsp92II CATG 3 cut(s) 1156, 1238, 1379
HspAI GCGC 4 cut(s) 92, 661, 929, 1321
KflI GGGWCCC 1 cut(s) 996
KspI CCGCGG 1 cut(s) 342
LmnI GCTCC 4 cut(s) 200, 668, 737, 865
Lsp1109I GCAGC 6 cut(s) 96, 213, 285, 322, 752, 1494
LweI GCATC 5 cut(s) 310, 1080, 1165, 1330, 1674
MaeI CTAG 2 cut(s) 701, 1442
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 4 cut(s) 535, 696, 787, 1136
MbiI CCGCTC 2 cut(s) 216, 545
MflI RGATCY 1 cut(s) 749
MhlI GDGCHC 1 cut(s) 870
MluCI AATT 7 cut(s) 10, 168, 252, 807, 1042, 1552, 1664
MlyI GAGTC 1 cut(s) 1679
MmeI TCCRAC 4 cut(s) 222, 330, 992, 1621
MroXI GAANNNNTTC 3 cut(s) 30, 566, 1271
MseI TTAA 6 cut(s) 842, 936, 1053, 1062, 1230, 1395
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 4 cut(s) 284, 335, 341, 644
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 3 cut(s) 121, 556, 1002
Mva1269I GAATGC 3 cut(s) 229, 301, 708
MvaI CCWGG 3 cut(s) 121, 556, 1002
MvnI CGCG 3 cut(s) 341, 428, 931
NlaIII CATG 3 cut(s) 1156, 1238, 1379
NlaIV GGNNCC 4 cut(s) 682, 751, 997, 998
NmeAIII GCCGAG 4 cut(s) 349, 430, 478, 808
NmuCI GTSAC 2 cut(s) 535, 696
NspI RCATGY 2 cut(s) 1156, 1238
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 3 cut(s) 229, 301, 708
PdmI GAANNNNTTC 3 cut(s) 30, 566, 1271
PfeI GAWTC 6 cut(s) 26, 31, 179, 272, 473, 575
Ple19I CGATCG 2 cut(s) 193, 222
PleI GAGTC 1 cut(s) 1678
PpsI GAGTC 1 cut(s) 1678
PpuMI RGGWCCY 2 cut(s) 681, 996
PsiI TTATAA 1 cut(s) 633
Psp5II RGGWCCY 2 cut(s) 681, 996
Psp6I CCWGG 3 cut(s) 119, 554, 1000
PspFI CCCAGC 2 cut(s) 992, 1658
PspGI CCWGG 3 cut(s) 119, 554, 1000
PspN4I GGNNCC 4 cut(s) 682, 751, 997, 998
PspPI GGNCC 4 cut(s) 238, 681, 763, 996
PspPPI RGGWCCY 2 cut(s) 681, 996
PsuI RGATCY 1 cut(s) 749
PvuI CGATCG 2 cut(s) 193, 222
PvuII CAGCTG 1 cut(s) 644
RgaI GCGATCGC 1 cut(s) 193
RsaI GTAC 1 cut(s) 1481
RsaNI GTAC 1 cut(s) 1480
RseI CAYNNNNRTG 1 cut(s) 438
SacII CCGCGG 1 cut(s) 342
SaqAI TTAA 6 cut(s) 842, 936, 1053, 1062, 1230, 1395
Sau96I GGNCC 4 cut(s) 238, 681, 763, 996
SchI GAGTC 1 cut(s) 1679
ScrFI CCNGG 3 cut(s) 121, 556, 1002
SduI GDGCHC 1 cut(s) 870
SfaAI GCGATCGC 1 cut(s) 193
SfaNI GCATC 5 cut(s) 310, 1080, 1165, 1330, 1674
Sfr303I CCGCGG 1 cut(s) 342
SgfI GCGATCGC 1 cut(s) 193
SgrBI CCGCGG 1 cut(s) 342
SinI GGWCC 4 cut(s) 238, 681, 763, 996
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 1 cut(s) 1252
SmoI CTYRAG 1 cut(s) 1252
Sse9I AATT 7 cut(s) 10, 168, 252, 807, 1042, 1552, 1664
SspI AATATT 1 cut(s) 1352
SspMI CTAG 2 cut(s) 701, 1442
StyD4I CCNGG 3 cut(s) 119, 554, 1000
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 5 cut(s) 130, 400, 583, 774, 1015
TaiI ACGT 1 cut(s) 210
TaqI TCGA 8 cut(s) 24, 29, 218, 222, 256, 423, 520, 1391
TaqII GACCGA 1 cut(s) 506
TasI AATT 7 cut(s) 10, 168, 252, 807, 1042, 1552, 1664
TatI WGTACW 1 cut(s) 1479
TauI GCSGC 6 cut(s) 335, 341, 413, 431, 545, 1701
TfiI GAWTC 6 cut(s) 26, 31, 179, 272, 473, 575
Tru1I TTAA 6 cut(s) 842, 936, 1053, 1062, 1230, 1395
Tru9I TTAA 6 cut(s) 842, 936, 1053, 1062, 1230, 1395
TscAI CASTG 7 cut(s) 24, 94, 586, 673, 1020, 1044, 1700
TseFI GTSAC 2 cut(s) 535, 696
TseI GCWGC 6 cut(s) 84, 226, 298, 335, 740, 1507
Tsp45I GTSAC 2 cut(s) 535, 696
TspDTI ATGAA 3 cut(s) 486, 1302, 1500
TspGWI ACGGA 6 cut(s) 284, 476, 523, 1216, 1320, 1377
TspRI CASTG 7 cut(s) 24, 94, 586, 673, 1020, 1044, 1700
VpaK11BI GGWCC 4 cut(s) 238, 681, 763, 996
XapI RAATTY 3 cut(s) 10, 252, 1552
XceI RCATGY 2 cut(s) 1156, 1238
XmnI GAANNNNTTC 3 cut(s) 30, 566, 1271
XspI CTAG 2 cut(s) 701, 1442
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.