Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004094.1

Orthogroup ID

OG0003103

Physical Location & Seq

Genomic Coordinates Forward (+)

151747 .. 170449

Gene Structure 18703 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3606 bp

atgtactataataagccattaattaagccctgccgtgtggagactcttcgctctccaacagcgaaatctcagagaaagagttggaagcagacaggcaggttgactttacgagtttaccgtcgcgtggatcctgaagtggacctcaatagtcaatatagcacttgtacggaccaatctatgagtcacccaataagcagctcgcctgaccgaccaaaccacgatttagaggccggaagcagtcgctcaggcaacgtgaacgacgatgagtcgtggaccgatccttttgatatgaacagtaccaagtatgcttctgttgaacgcctcaagcgttggaggaaagcagcacttgtactgaattcttctcgccgattccgatacaccctcaacttgaaaaaggaagaagagaagaaacagacactgaggaagataagagcacgtgcacaagccatacgagctgcatctttatacgcagaagttggaaatcccctgcaaggaactttacaaccaatagcatccgctgctagcgattttcagattgcccatgagcaacttgtttcagtgatcagggaccgtgacttacatgctatgcaacaatatgggggagtcagtggcttagcagatttattgaaaaccaatctacacaagggaattcttggtgatagtgctgattgtctaaaccggaaaaatgcatttggatccaacacatatcctcctggaagagcaaggagtttctggactattctttgggaagcctggcaagaccttactctgatcattttgatgatagcagcagtggctaccttagggctacatataaatatgaaggatatgaaggaggaatggtatggtggtggcagcattgctttagcagttattcttgtcattgttgtcatagagaggagaaatatacatttagaggtcatcagaggtggtagacgagttgaggtttctatttatgatattgtcgttggtgatgttgtaccgcttagcattggtgatcaggtccctgctgatggtattttaatcactggctactcacttgctacagaggagacccaaatcattggggatagcaagattgtgagccttcatgcatgttcggttcacaaggactgcaaggacccatttctgatgtctggctgcaaaattgcaaatgggagtggtacaatgctggtaactagtgtagggatgaatactgaatggggtttgctcatggctagtatttcagaagatattggtgaagaaacacctttgcaggtgggtttaaatggagttgctactgtcattggactttttgggatcgtaatagctcttttcgtcttcaatgtcgatttagttagattatttaccggccattcaaggaatccagatggaactagtcagtttgtacccggcaagactggatttgaaaaagccattcaatttactatcattgtagtgaccaatgtaatggttgctgtacctgaagggctccctattgctgtcacgttaatccttgtctattccatgaccaaaatgatggcagataagatcttggtgcggaggcaatctgcatgcgaggctataggctctgccacaactatttgcattgataagactggacttttaacttcgaaccagatgactgttgttaaggcttatgttggtgggaagaaaatctatccttcagacaataagtcgcgattgtctccaatgctatggtcgttaattattgaaagcattgcacagaacacaaatggcagtgtctacatggctgagaatggcggtgatgtagaggtttatggatcaccaactgaaaaagcaattctgcgctgggggattaagattggaatgaattttgaagccacgaagtctgaatctacagttcttcatgcgctcgcatttaactcagtgaagaaacgaagtgctgttgcgcttaaattggccaattctcaagttcatatacactggaaaggggctgctgaaacaatcttggcctcatgcacaaagtacattgatgctaatgatcacttggtagcaatggatgaagataagttaatgtttttcaatgaagctattgagggtatggcttctggaagtctgcggtgtgttgctattgcatataggttatacgagtttgaaaatgttccgactgatgaacaactagttcagtgggcattacctgaggatgaccttattttacttgctattgttggtatagaggatccttgtcagccaggagccagagatgcagtgcgacaatgccaaaatgctggtgttaaggtatgcatggttacgggtgacaatcttcaaacagcaaaagcaattgctttggagtgtggcatacttggttccgaggcagatgctactgaacaaaatatcattgaaggaagagtatttcgtgccttttctgataagcaaagagaagatttagctgagaggatatcagtgatgggacgatcttcacccaatgacaagcttcttcttgtgcaagcactgaagaaaaggggacatgttgttgctgtcactggtgatggttgtaatgatgctccggcattatatgaggccgatattggtcttgctatgggtattcaaggggctgaagtagctaaagagagttcagatataatcatcttggataataacttttctacaattgtgaaggctatcaaatggggtcgatctgtatattcaaatattcagaaatttacccagttccatcttacagttggacttgcagcattaatcataaatgtcattgcagaagtttcctctggttcagctccattaaatgctgtgcagattctctgggtaagcattatcatggacactcaaggagcactagtattgcgtactgagccaccatctgataacctgatgcggaagcctcctgttgatcgaaaggcacctcttattacaaatatagtgtggaggaacttgttggttcagtctttctaccaagtcgctatcttgcttattctaaatttccacggaaggaatttgctgaatttggaacttgctgattacaatgtgatgaatacaatgatattcaatgcttttgtcctttgtcaaattttcaatgaattcaatgctcgaaagcccgaggaatataacattttcagaggaattacttcaaactatctctttctgggaatagtgggaataactcttgtgcttcagattgttgccattgagtttcttggcaaatttacttcaactgtgaggcttaacttgaagcagtggctgatttccatcatcattggttttatctgttggccccttgatgctatcgggaaactcttaccagttccaaaaactcccttcatcaagtttgccttgaagctttggggcgatcgcttttccagcatctcacctagtctgtttgattggttggcggcggttctggatggtttggattcacaacgtgctgatgttttctatgtctctctctctgggccatctggtgtgagaggaataatatgctctactagaacaggcggtgtaggagtgatcaaaagggatgagtttggacactgtgccgccttagccaagcatgctcgctcagccttgcaggtagaggctgaagccatgcgagccggcttgctcgattcatcagcaatggagtga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1201

Amino Acids

131.81

Weight (kDa)

7.5

Isoelectric Point (pI)

40.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	93 - 135	1.9e-15	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	199 - 265	4.9e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	306 - 407	2.9e-14	P-type ATPase actuator domain
Cation_ATPase	PF13246	579 - 739	5.1e-28	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	719 - 849	1.9e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	921 - 1094	9.3e-42	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29900 AT4G29900 AT5G57110 AT5G57110 AT5G57110
fragaria_vesca	FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940 FvH4_4g06940
malus_domestica	MD13G1241000.v1.1 MD16G1245600.v1.1 MD16G1246100.v1.1
prunus_persica	Prupe.1G072600_v2.0.a1 Prupe.8G012900_v2.0.a1
pyrus_communis	pycom13g21220
rosa_chinensis	RchiOBHm_Chr4g0398891 RchiOBHm_Chr4g0398951
rosa_laevigata	RLG00000009307 RLG00000009309 RLG00000009365
rosa_multiflora	Rmu_sc0004094.1_g000033 Rmu_ssc0000064.1_g000006
rosa_roxburghii	Rroxscaffold_5G00343340 Rroxscaffold_5G00343930 Rroxscaffold_5G00344000
rosa_rugosa	Rorug04G0013200
rosa_samantha	Rh4AG091100 Rh4CG093100 Rh4CG099100 Rh4CG099400
rosa_wichuraiana	Rw4G007100 Rw4G007130 Rw4G007500 Rw4G007540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1246
Acc36I	ACCTGC	3 cut(s)	87, 1246, 3541
AccB1I	GGYRCC	1 cut(s)	2884
AccI	GTMKAC	2 cut(s)	934, 1746
AccII	CGCG	2 cut(s)	123, 1681
AclWI	GGATC	9 cut(s)	122, 135, 272, 690, 703, 1307, 1792, 2198, 2211
AcoI	YGGCCR	2 cut(s)	1351, 1923
AcuI	CTGAAG	7 cut(s)	153, 1485, 1650, 2498, 2601, 3140, 3582
AcvI	CACGTG	1 cut(s)	437
AdeI	CACNNNGTG	1 cut(s)	3404
AfiI	CCNNNNNNNGG	3 cut(s)	124, 491, 1013
AflIII	ACRYGT	1 cut(s)	2491
AhdI	GACNNNNNGTC	2 cut(s)	265, 1675
AhlI	ACTAGT	4 cut(s)	1178, 1376, 2143, 2821
AjnI	CCWGG	3 cut(s)	712, 752, 2215
AluBI	AGCT	9 cut(s)	198, 455, 1310, 2054, 2414, 2458, 2588, 2762, 3322
AluI	AGCT	9 cut(s)	198, 455, 1310, 2054, 2414, 2458, 2588, 2762, 3322
Alw21I	GWGCWC	3 cut(s)	436, 442, 2821
Alw26I	GTCTC	4 cut(s)	35, 1046, 1692, 3427
Alw44I	GTGCAC	1 cut(s)	438
AlwI	GGATC	9 cut(s)	122, 135, 272, 690, 703, 1307, 1792, 2198, 2211
AlwNI	CAGNNNCTG	2 cut(s)	418, 3223
Ama87I	CYCGRG	1 cut(s)	3080
AoxI	GGCC	7 cut(s)	228, 1351, 1923, 1974, 2544, 3254, 3434
ApaLI	GTGCAC	1 cut(s)	438
ApeKI	GCWGC	9 cut(s)	195, 341, 455, 518, 788, 855, 1140, 1958, 2717
AseI	ATTAAT	2 cut(s)	20, 2723
AsiSI	GCGATCGC	1 cut(s)	3334
Asp700I	GAANNNNTTC	2 cut(s)	2124, 3109
AspLEI	GCGC	3 cut(s)	1812, 1876, 1915
AspS9I	GGNCC	8 cut(s)	139, 169, 273, 568, 1003, 1120, 3255, 3434
AsuC2I	CCSGG	1 cut(s)	1392
AsuII	TTCGAA	1 cut(s)	1613
AsuNHI	GCTAGC	1 cut(s)	521
AvaI	CYCGRG	1 cut(s)	3080
AvaII	GGWCC	6 cut(s)	139, 169, 273, 568, 1003, 1120
AxyI	CCTNAGG	2 cut(s)	802, 2163
BaeGI	GKGCMC	1 cut(s)	442
BalI	TGGCCA	1 cut(s)	1925
BamHI	GGATCC	3 cut(s)	127, 695, 2203
BanI	GGYRCC	1 cut(s)	2884
BanII	GRGCYC	1 cut(s)	1473
BbrPI	CACGTG	1 cut(s)	437
BbsI	GAAGAC	1 cut(s)	1312
Bbv12I	GWGCWC	3 cut(s)	436, 442, 2821
BbvI	GCAGC	9 cut(s)	207, 353, 442, 505, 800, 867, 1127, 1945, 2729
BceAI	ACGGC	1 cut(s)	18
BcgI	CGANNNNNNTGC	2 cut(s)	2324, 2358
BciT130I	CCWGG	3 cut(s)	714, 754, 2217
BclI	TGATCA	5 cut(s)	561, 771, 997, 2005, 3489
BcnI	CCSGG	1 cut(s)	1392
BcoDI	GTCTC	4 cut(s)	35, 1046, 1692, 3427
BcuI	ACTAGT	4 cut(s)	1178, 1376, 2143, 2821
BfaI	CTAG	8 cut(s)	522, 1179, 1218, 1377, 2144, 2822, 3354, 3468
BfmI	CTRYAG	3 cut(s)	1044, 1563, 1860
BfuAI	ACCTGC	3 cut(s)	87, 1246, 3541
BglII	AGATCT	1 cut(s)	1530
BlpI	GCTNAGC	3 cut(s)	613, 986, 3541
Bme1390I	CCNGG	4 cut(s)	714, 754, 1392, 2217
Bme18I	GGWCC	6 cut(s)	139, 169, 273, 568, 1003, 1120
BmeRI	GACNNNNNGTC	2 cut(s)	265, 1675
BmeT110I	CYCGRG	1 cut(s)	3080
BmgT120I	GGNCC	8 cut(s)	139, 169, 273, 568, 1003, 1120, 3255, 3434
BmrFI	CCNGG	4 cut(s)	714, 754, 1392, 2217
BmrI	ACTGGG	1 cut(s)	2686
BmsI	GCATC	9 cut(s)	467, 521, 1987, 2218, 2332, 2515, 2847, 3253, 3354
BmtI	GCTAGC	1 cut(s)	525
BmuI	ACTGGG	1 cut(s)	2686
BpiI	GAAGAC	1 cut(s)	1312
Bpu10I	CCTNAGC	2 cut(s)	244, 3523
Bpu1102I	GCTNAGC	3 cut(s)	613, 986, 3541
Bpu14I	TTCGAA	1 cut(s)	1613
BpuEI	CTTGAG	3 cut(s)	308, 1917, 2796
BpuMI	CCSGG	1 cut(s)	1392
BsaAI	YACGTR	1 cut(s)	437
BsaBI	GATNNNNATC	1 cut(s)	3230
BsaI	GGTCTC	1 cut(s)	1046
BsaJI	CCNNGG	3 cut(s)	2334, 2968, 3081
BsaWI	WCCGGW	1 cut(s)	678
BsaXI	ACNNNNNCTCC	6 cut(s)	694, 724, 827, 857, 2808, 2838
Bsc4I	CCNNNNNNNGG	3 cut(s)	124, 491, 1013
Bse118I	RCCGGY	2 cut(s)	1349, 3575
Bse1I	ACTGG	7 cut(s)	1033, 1405, 1603, 1952, 2512, 2692, 3284
Bse21I	CCTNAGG	2 cut(s)	802, 2163
Bse3DI	GCAATG	5 cut(s)	858, 1719, 2025, 2736, 3603
Bse8I	GATNNNNATC	1 cut(s)	3230
BseBI	CCWGG	3 cut(s)	714, 754, 2217
BseDI	CCNNGG	3 cut(s)	2334, 2968, 3081
BseGI	GGATG	6 cut(s)	512, 1194, 2029, 2173, 3391, 3505
BseJI	GATNNNNATC	1 cut(s)	3230
BseLI	CCNNNNNNNGG	3 cut(s)	124, 491, 1013
BseMI	GCAATG	5 cut(s)	858, 1719, 2025, 2736, 3603
BseMII	CTCAG	9 cut(s)	83, 258, 410, 1746, 1902, 2154, 2406, 2826, 3555
BseNI	ACTGG	7 cut(s)	1033, 1405, 1603, 1952, 2512, 2692, 3284
BseRI	GAGGAG	2 cut(s)	913, 1064
BseSI	GKGCMC	1 cut(s)	442
BseXI	GCAGC	9 cut(s)	207, 353, 442, 505, 800, 867, 1127, 1945, 2729
BseYI	CCCAGC	1 cut(s)	1812
BsgI	GTGCAG	1 cut(s)	2798
Bsh1236I	CGCG	2 cut(s)	123, 1681
Bsh1285I	CGRYCG	1 cut(s)	3334
BshFI	GGCC	7 cut(s)	230, 1353, 1925, 1976, 2546, 3256, 3436
BshNI	GGYRCC	1 cut(s)	2884
BsiEI	CGRYCG	1 cut(s)	3334
BsiHKAI	GWGCWC	3 cut(s)	436, 442, 2821
BsiHKCI	CYCGRG	1 cut(s)	3080
BsiSI	CCGG	6 cut(s)	231, 679, 1350, 1392, 2531, 3576
BslFI	GGGAC	4 cut(s)	581, 989, 2448, 2502
BslI	CCNNNNNNNGG	3 cut(s)	124, 491, 1013
BsmAI	GTCTC	4 cut(s)	35, 1046, 1692, 3427
BsmFI	GGGAC	4 cut(s)	581, 989, 2448, 2502
BsnI	GGCC	7 cut(s)	230, 1353, 1925, 1976, 2546, 3256, 3436
Bso31I	GGTCTC	1 cut(s)	1046
BsoBI	CYCGRG	1 cut(s)	3080
Bsp119I	TTCGAA	1 cut(s)	1613
Bsp1286I	GDGCHC	4 cut(s)	436, 442, 1473, 2821
Bsp1720I	GCTNAGC	3 cut(s)	613, 986, 3541
Bsp68I	TCGCGA	1 cut(s)	1681
BspANI	GGCC	7 cut(s)	230, 1353, 1925, 1976, 2546, 3256, 3436
BspCNI	CTCAG	9 cut(s)	82, 257, 411, 1747, 1901, 2155, 2407, 2827, 3554
BspFNI	CGCG	2 cut(s)	123, 1681
BspMI	ACCTGC	3 cut(s)	87, 1246, 3541
BspOI	GCTAGC	1 cut(s)	525
BspPI	GGATC	9 cut(s)	122, 135, 272, 690, 703, 1307, 1792, 2198, 2211
BspQI	GCTCTTC	1 cut(s)	712
BspT104I	TTCGAA	1 cut(s)	1613
BspT107I	GGYRCC	1 cut(s)	2884
BspTNI	GGTCTC	1 cut(s)	1046
BsrDI	GCAATG	5 cut(s)	858, 1719, 2025, 2736, 3603
BsrFI	RCCGGY	2 cut(s)	1349, 3575
BsrI	ACTGG	7 cut(s)	1033, 1405, 1603, 1952, 2512, 2692, 3284
BssAI	RCCGGY	2 cut(s)	1349, 3575
BssECI	CCNNGG	3 cut(s)	2334, 2968, 3081
Bst2UI	CCWGG	3 cut(s)	714, 754, 2217
Bst4CI	ACNGT	9 cut(s)	119, 296, 572, 1282, 1627, 1864, 2707, 3199, 3515
Bst6I	CTCTTC	4 cut(s)	51, 396, 712, 2365
BstAPI	GCANNNNNTGC	1 cut(s)	518
BstBAI	YACGTR	1 cut(s)	437
BstBI	TTCGAA	1 cut(s)	1613
BstDSI	CCRYGG	1 cut(s)	2968
BstF5I	GGATG	6 cut(s)	512, 1194, 2029, 2173, 3391, 3505
BstFNI	CGCG	2 cut(s)	123, 1681
BstHHI	GCGC	3 cut(s)	1812, 1876, 1915
BstMAI	GTCTC	4 cut(s)	35, 1046, 1692, 3427
BstMCI	CGRYCG	1 cut(s)	3334
BstNI	CCWGG	3 cut(s)	714, 754, 2217
BstNSI	RCATGY	5 cut(s)	584, 1098, 1557, 2495, 3536
BstSCI	CCNGG	4 cut(s)	712, 752, 1390, 2215
BstSFI	CTRYAG	3 cut(s)	1044, 1563, 1860
BstSLI	GKGCMC	1 cut(s)	442
BstUI	CGCG	2 cut(s)	123, 1681
BstV1I	GCAGC	9 cut(s)	207, 353, 442, 505, 800, 867, 1127, 1945, 2729
BstV2I	GAAGAC	1 cut(s)	1312
BstX2I	RGATCY	4 cut(s)	127, 695, 1530, 2203
BstXI	CCANNNNNNTGG	5 cut(s)	1064, 1450, 1519, 1698, 2252
BstYI	RGATCY	4 cut(s)	127, 695, 1530, 2203
Bsu36I	CCTNAGG	2 cut(s)	802, 2163
BsuRI	GGCC	7 cut(s)	230, 1353, 1925, 1976, 2546, 3256, 3436
BtgI	CCRYGG	1 cut(s)	2968
BtsCI	GGATG	6 cut(s)	512, 1194, 2029, 2173, 3391, 3505
BtsI	GCAGTG	4 cut(s)	798, 1747, 2238, 3224
BtuMI	TCGCGA	1 cut(s)	1681
BveI	ACCTGC	3 cut(s)	87, 1246, 3541
CaiI	CAGNNNCTG	2 cut(s)	418, 3223
CfoI	GCGC	3 cut(s)	1812, 1876, 1915
Cfr10I	RCCGGY	2 cut(s)	1349, 3575
Cfr13I	GGNCC	8 cut(s)	139, 169, 273, 568, 1003, 1120, 3255, 3434
DraI	TTTAAA	1 cut(s)	1266
DraIII	CACNNNGTG	1 cut(s)	3404
DriI	GACNNNNNGTC	2 cut(s)	265, 1675
EaeI	YGGCCR	2 cut(s)	1351, 1923
Eam1104I	CTCTTC	4 cut(s)	51, 396, 712, 2365
Eam1105I	GACNNNNNGTC	2 cut(s)	265, 1675
EarI	CTCTTC	4 cut(s)	51, 396, 712, 2365
Eco24I	GRGCYC	1 cut(s)	1473
Eco31I	GGTCTC	1 cut(s)	1046
Eco32I	GATATC	1 cut(s)	2424
Eco47I	GGWCC	6 cut(s)	139, 169, 273, 568, 1003, 1120
Eco57I	CTGAAG	7 cut(s)	153, 1485, 1650, 2498, 2601, 3140, 3582
Eco72I	CACGTG	1 cut(s)	437
Eco81I	CCTNAGG	2 cut(s)	802, 2163
Eco88I	CYCGRG	1 cut(s)	3080
EcoO109I	RGGNCCY	2 cut(s)	1003, 1120
EcoRI	GAATTC	3 cut(s)	355, 648, 3062
EcoRII	CCWGG	3 cut(s)	712, 752, 2215
EcoRV	GATATC	1 cut(s)	2424
EcoT22I	ATGCAT	3 cut(s)	691, 1096, 2270
EcoT38I	GRGCYC	1 cut(s)	1473
FalI	AAGNNNNNCTT	4 cut(s)	330, 362, 3299, 3331
FaqI	GGGAC	4 cut(s)	581, 989, 2448, 2502
FbaI	TGATCA	5 cut(s)	561, 771, 997, 2005, 3489
FblI	GTMKAC	2 cut(s)	934, 1746
FokI	GGATG	6 cut(s)	499, 1201, 2036, 2180, 3398, 3512
FriOI	GRGCYC	1 cut(s)	1473
FspBI	CTAG	8 cut(s)	522, 1179, 1218, 1377, 2144, 2822, 3354, 3468
GlaI	GCGC	3 cut(s)	1811, 1875, 1914
GsaI	CCCAGC	1 cut(s)	1816
HaeIII	GGCC	7 cut(s)	230, 1353, 1925, 1976, 2546, 3256, 3436
HapII	CCGG	6 cut(s)	231, 679, 1350, 1392, 2531, 3576
HhaI	GCGC	3 cut(s)	1812, 1876, 1915
Hin6I	GCGC	3 cut(s)	1810, 1874, 1913
HinP1I	GCGC	3 cut(s)	1810, 1874, 1913
HincII	GTYRAC	1 cut(s)	102
HindII	GTYRAC	1 cut(s)	102
HindIII	AAGCTT	2 cut(s)	2456, 3320
HpaII	CCGG	6 cut(s)	231, 679, 1350, 1392, 2531, 3576
Hpy166II	GTNNAC	9 cut(s)	102, 115, 139, 256, 273, 440, 935, 1105, 1747
Hpy188III	TCNNGA	7 cut(s)	131, 733, 1367, 1680, 2073, 3271, 3383
Hpy8I	GTNNAC	9 cut(s)	102, 115, 139, 256, 273, 440, 935, 1105, 1747
Hpy99I	CGWCG	2 cut(s)	123, 263
HpyAV	CCTTC	9 cut(s)	817, 826, 1097, 1460, 1674, 2360, 2635, 2967, 3310
HpyCH4III	ACNGT	9 cut(s)	119, 296, 572, 1282, 1627, 1864, 2707, 3199, 3515
HpyCH4IV	ACGT	4 cut(s)	252, 436, 1487, 3403
HpySE526I	ACGT	4 cut(s)	252, 436, 1487, 3403
HspAI	GCGC	3 cut(s)	1810, 1874, 1913
KroI	GCCGGC	1 cut(s)	3575
KroNI	GCCGGC	1 cut(s)	3577
Ksp22I	TGATCA	5 cut(s)	561, 771, 997, 2005, 3489
LguI	GCTCTTC	1 cut(s)	712
LmnI	GCTCC	5 cut(s)	1476, 2219, 2533, 2767, 2816
Lsp1109I	GCAGC	9 cut(s)	207, 353, 442, 505, 800, 867, 1127, 1945, 2729
LweI	GCATC	9 cut(s)	467, 521, 1987, 2218, 2332, 2515, 2847, 3253, 3354
MaeI	CTAG	8 cut(s)	522, 1179, 1218, 1377, 2144, 2822, 3354, 3468
MaeII	ACGT	4 cut(s)	252, 436, 1487, 3403
MaeIII	GTNAC	8 cut(s)	182, 572, 1174, 1438, 1483, 2272, 2279, 2503
MfeI	CAATTG	2 cut(s)	2304, 2634
MflI	RGATCY	4 cut(s)	127, 695, 1530, 2203
MhlI	GDGCHC	4 cut(s)	436, 442, 1473, 2821
MlsI	TGGCCA	1 cut(s)	1925
MluNI	TGGCCA	1 cut(s)	1925
MlyI	GAGTC	4 cut(s)	37, 190, 275, 612
MmeI	TCCRAC	7 cut(s)	62, 80, 311, 457, 723, 2153, 2689
Mox20I	TGGCCA	1 cut(s)	1925
Mph1103I	ATGCAT	3 cut(s)	691, 1096, 2270
MroNI	GCCGGC	1 cut(s)	3575
MroXI	GAANNNNTTC	2 cut(s)	2124, 3109
MscI	TGGCCA	1 cut(s)	1925
MslI	CAYNNNNRTG	3 cut(s)	779, 1436, 2801
Msp20I	TGGCCA	1 cut(s)	1925
MspA1I	CMGCKG	1 cut(s)	518
MspI	CCGG	6 cut(s)	231, 679, 1350, 1392, 2531, 3576
MspR9I	CCNGG	4 cut(s)	714, 754, 1392, 2217
MunI	CAATTG	2 cut(s)	2304, 2634
MvaI	CCWGG	3 cut(s)	714, 754, 2217
MvnI	CGCG	2 cut(s)	123, 1681
NaeI	GCCGGC	1 cut(s)	3577
NciI	CCSGG	1 cut(s)	1392
NgoMIV	GCCGGC	1 cut(s)	3575
NheI	GCTAGC	1 cut(s)	521
NmuCI	GTSAC	6 cut(s)	182, 572, 1438, 1483, 2279, 2503
NruI	TCGCGA	1 cut(s)	1681
NsiI	ATGCAT	3 cut(s)	691, 1096, 2270
NspI	RCATGY	5 cut(s)	584, 1098, 1557, 2495, 3536
NspV	TTCGAA	1 cut(s)	1613
PacI	TTAATTAA	1 cut(s)	24
PaeI	GCATGC	2 cut(s)	1557, 3536
PaqCI	CACCTGC	1 cut(s)	1246
PciI	ACATGT	1 cut(s)	2491
PciSI	GCTCTTC	1 cut(s)	712
PcsI	WCGNNNNNNNCGW	4 cut(s)	115, 258, 325, 370
PdiI	GCCGGC	1 cut(s)	3577
PdmI	GAANNNNTTC	2 cut(s)	2124, 3109
PfeI	GAWTC	6 cut(s)	369, 1363, 1856, 2782, 3395, 3587
PfoI	TCCNGGA	1 cut(s)	712
Ple19I	CGATCG	1 cut(s)	3334
PleI	GAGTC	4 cut(s)	37, 189, 274, 611
PmaCI	CACGTG	1 cut(s)	437
PmlI	CACGTG	1 cut(s)	437
PpsI	GAGTC	4 cut(s)	37, 189, 274, 611
Ppu21I	YACGTR	1 cut(s)	437
PpuMI	RGGWCCY	2 cut(s)	1003, 1120
PscI	ACATGT	1 cut(s)	2491
PshBI	ATTAAT	2 cut(s)	20, 2723
Psp5II	RGGWCCY	2 cut(s)	1003, 1120
Psp6I	CCWGG	3 cut(s)	712, 752, 2215
PspCI	CACGTG	1 cut(s)	437
PspFI	CCCAGC	1 cut(s)	1812
PspGI	CCWGG	3 cut(s)	712, 752, 2215
PspPI	GGNCC	8 cut(s)	139, 169, 273, 568, 1003, 1120, 3255, 3434
PspPPI	RGGWCCY	2 cut(s)	1003, 1120
PstNI	CAGNNNCTG	2 cut(s)	418, 3223
PsuI	RGATCY	4 cut(s)	127, 695, 1530, 2203
PvuI	CGATCG	1 cut(s)	3334
RgaI	GCGATCGC	1 cut(s)	3334
RruI	TCGCGA	1 cut(s)	1681
RseI	CAYNNNNRTG	3 cut(s)	779, 1436, 2801
SapI	GCTCTTC	1 cut(s)	712
Sau96I	GGNCC	8 cut(s)	139, 169, 273, 568, 1003, 1120, 3255, 3434
SchI	GAGTC	4 cut(s)	37, 190, 275, 612
ScrFI	CCNGG	4 cut(s)	714, 754, 1392, 2217
SduI	GDGCHC	4 cut(s)	436, 442, 1473, 2821
SfaAI	GCGATCGC	1 cut(s)	3334
SfaNI	GCATC	9 cut(s)	467, 521, 1987, 2218, 2332, 2515, 2847, 3253, 3354
SfcI	CTRYAG	3 cut(s)	1044, 1563, 1860
SfuI	TTCGAA	1 cut(s)	1613
SgfI	GCGATCGC	1 cut(s)	3334
SinI	GGWCC	6 cut(s)	139, 169, 273, 568, 1003, 1120
SmiMI	CAYNNNNRTG	3 cut(s)	779, 1436, 2801
SmlI	CTYRAG	3 cut(s)	323, 1932, 2811
SmoI	CTYRAG	3 cut(s)	323, 1932, 2811
SpeI	ACTAGT	4 cut(s)	1178, 1376, 2143, 2821
SphI	GCATGC	2 cut(s)	1557, 3536
SspI	AATATT	1 cut(s)	2677
SspMI	CTAG	8 cut(s)	522, 1179, 1218, 1377, 2144, 2822, 3354, 3468
StyD4I	CCNGG	4 cut(s)	712, 752, 1390, 2215
TaaI	ACNGT	9 cut(s)	119, 296, 572, 1282, 1627, 1864, 2707, 3199, 3515
TaiI	ACGT	4 cut(s)	255, 439, 1490, 3406
TaqI	TCGA	6 cut(s)	1329, 1613, 2659, 2878, 3073, 3585
TaqII	GACCGA	2 cut(s)	222, 290
TatI	WGTACW	3 cut(s)	3, 349, 1989
TauI	GCSGC	2 cut(s)	3377, 3521
TfiI	GAWTC	6 cut(s)	369, 1363, 1856, 2782, 3395, 3587
TseFI	GTSAC	6 cut(s)	182, 572, 1438, 1483, 2279, 2503
TseI	GCWGC	9 cut(s)	195, 341, 455, 518, 788, 855, 1140, 1958, 2717
Tsp45I	GTSAC	6 cut(s)	182, 572, 1438, 1483, 2279, 2503
TspGWI	ACGGA	2 cut(s)	182, 2985
VneI	GTGCAC	1 cut(s)	438
VpaK11BI	GGWCC	6 cut(s)	139, 169, 273, 568, 1003, 1120
VspI	ATTAAT	2 cut(s)	20, 2723
XceI	RCATGY	5 cut(s)	584, 1098, 1557, 2495, 3536
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2705
XmiI	GTMKAC	2 cut(s)	934, 1746
XmnI	GAANNNNTTC	2 cut(s)	2124, 3109
XspI	CTAG	8 cut(s)	522, 1179, 1218, 1377, 2144, 2822, 3354, 3468
Zsp2I	ATGCAT	3 cut(s)	691, 1096, 2270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.