Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004220.1

Orthogroup ID

OG0005234

Physical Location & Seq

Genomic Coordinates Forward (+)

14688 .. 18324

Gene Structure 3637 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0004220.1_g000005.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

atggtttcgaccattgcattgcaggcagagaaggagccaattttgagtagcttcttgtacgcgagcatcttggcgcatgattgtttagagcaggcgttggggtttgttcttgcaaacaggttgcagaacccgacacttctggctactcagctgatggatatattcactaatgtaatgatgcatgacagagacattcagcgatctgctcggctggacgtgcaggcgtttaaagaacgtgatccagcttgtttgtcttactgttcggctctactgtatctcaagggttaccatgctctgcaagtatatcgagtagctcattcactgtggagccaaggacgcaaagtattggccttggcattacaaagccgagttagcgaggtgtttggagtggacattcaccctgttgttcaggagaaagaaaaggagagctgggaagctgcctctcggggactgaaaacaaagctggaagtcgctgagagcaatcggatatgtgctgaaattgaagtagccaaaatgagaagtcagctggaatcagaggtgtctgcacaaacccgaatgcttgatgcaagagatgctgaacttgcaactgccaaggaggagatcaaccgccttaaaaatgaattttcttcttacaaagtacgtgctcatgcacttctccaaaagaaggatgcagatctagctgcagctaaagactctgaacaagttacagctcttgaggaagcactaaaagaagctgaaaaagaagtttcgtctctgagtgctgaaagagataaggcccttcaggatcttcaggatgccttgactaattataataaagaactagcagaaagagatgcagctcttaatgataccatgcagcagatcaagagcttagaaaaaaagctcgagtctactaatgcccatcaccgatccgccaaagaagcatgggaagttaaccttagtaatttggaagaaacatggcgggcaagatgtgaaacattaagagctcaaaacgaagcatcttctggagaagacataaaaaaagaattggaagagctcaaattacggtataaaaaattgaaggaagaacacagttcatttcgtgatcttgctgatagaatgatggaggagaaggacaaagaaatttctagacttgtagatgataataagaaccttcatcaatctttggagtcaagaccaaaggttgatcataacgataattatagcacaggcatgtataaacaggatccatcaagtttaagcacctctgcgttagaacagcagattctgattctggcaaggcaacaagcgcaaagagaggaagagctagcacagtcacagaggcacattttagcgcttcaagaggaaattgaggagctggagcatgaaaatcgtctgcatagccaacaggtatcaagaagcctggatatttaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000793 GO:0000794 GO:0000795 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005794 GO:0005802 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006810 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0016043 GO:0016192 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0031984 GO:0034641 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048193 GO:0048285 GO:0051179 GO:0051234 GO:0051321 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0098791 GO:0099086 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRIP

Protein Analysis

470

Amino Acids

53.68

Weight (kDa)

5.39

Isoelectric Point (pI)

51.44

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005234)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17640 AT4G35640
fragaria_vesca	FvH4_1g10540 FvH4_1g10540 FvH4_1g10540 FvH4_1g10540
malus_domestica	MD02G1119500.v1.1 MD02G1119600.v1.1 MD15G1233400.v1.1
prunus_persica	Prupe.7G179500_v2.0.a1
pyrus_communis	pycom02g09310 pycom15g20790
rosa_chinensis	RchiOBHm_Chr2g0097471 RchiOBHm_Chr2g0097571 RchiOBHm_Chr2g0097591
rosa_multiflora	Rmu_co8255597.1_g000001 Rmu_co8464327.1_g000001 Rmu_sc0004220.1_g000005 Rmu_sc0005347.1_g000010 Rmu_sc0027623.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256370
rosa_rugosa	Rorug02G0067500 Rorug02G0067600 Rorug02G0067600
rosa_samantha	Rh2AG113900 Rh2BG116700 Rh2CG118700 Rh2DG117700
rosa_wichuraiana	Rw2G008910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	810
AccI	GTMKAC	1 cut(s)	890
AccII	CGCG	1 cut(s)	62
AciI	CCGC	3 cut(s)	607, 912, 961
AclWI	GGATC	5 cut(s)	233, 792, 903, 1220, 1233
AcsI	RAATTY	2 cut(s)	620, 1122
AcuI	CTGAAG	2 cut(s)	764, 773
AfaI	GTAC	2 cut(s)	59, 639
AfeI	AGCGCT	1 cut(s)	1335
AfiI	CCNNNNNNNGG	1 cut(s)	664
AgsI	TTSAA	3 cut(s)	503, 1060, 1340
AjiI	CACGTC	1 cut(s)	217
AjnI	CCWGG	1 cut(s)	1401
Alw21I	GWGCWC	3 cut(s)	646, 988, 1038
Alw26I	GTCTC	2 cut(s)	183, 756
AlwI	GGATC	5 cut(s)	233, 792, 903, 1220, 1233
AlwNI	CAGNNNCTG	1 cut(s)	1267
Ama87I	CYCGRG	2 cut(s)	444, 884
Aor51HI	AGCGCT	1 cut(s)	1335
AoxI	GGCC	2 cut(s)	348, 774
ApeKI	GCWGC	5 cut(s)	437, 680, 683, 836, 856
ApoI	RAATTY	2 cut(s)	620, 1122
AspLEI	GCGC	3 cut(s)	76, 1291, 1336
AspS9I	GGNCC	1 cut(s)	775
AsuHPI	GGTGA	2 cut(s)	389, 896
AsuNHI	GCTAGC	1 cut(s)	1306
AvaI	CYCGRG	2 cut(s)	444, 884
BamHI	GGATCC	1 cut(s)	1225
BanII	GRGCYC	2 cut(s)	988, 1038
BbsI	GAAGAC	1 cut(s)	1017
Bbv12I	GWGCWC	3 cut(s)	646, 988, 1038
BbvI	GCAGC	5 cut(s)	424, 667, 695, 848, 868
BccI	CCATC	4 cut(s)	148, 909, 1096, 1237
BcgI	CGANNNNNNTGC	4 cut(s)	287, 321, 1352, 1386
BciT130I	CCWGG	1 cut(s)	1403
BclI	TGATCA	1 cut(s)	1186
BcoDI	GTCTC	2 cut(s)	183, 756
BfaI	CTAG	4 cut(s)	677, 821, 1128, 1307
BfmI	CTRYAG	1 cut(s)	681
BfoI	RGCGCY	1 cut(s)	1337
BglII	AGATCT	1 cut(s)	673
BisI	GCNGC	5 cut(s)	438, 681, 684, 837, 857
BlsI	GCNGC	5 cut(s)	439, 682, 685, 838, 858
Bme1390I	CCNGG	1 cut(s)	1403
BmeT110I	CYCGRG	2 cut(s)	444, 884
BmgBI	CACGTC	1 cut(s)	217
BmgT120I	GGNCC	1 cut(s)	775
BmiI	GGNNCC	3 cut(s)	36, 329, 1227
BmrFI	CCNGG	1 cut(s)	1403
BmsI	GCATC	8 cut(s)	75, 168, 553, 562, 658, 784, 823, 1007
BmtI	GCTAGC	1 cut(s)	1310
BpiI	GAAGAC	1 cut(s)	1017
BpmI	CTGGAG	2 cut(s)	1026, 1379
BpuEI	CTTGAG	2 cut(s)	263, 734
BsaAI	YACGTR	1 cut(s)	641
BsaBI	GATNNNNATC	1 cut(s)	672
BsaJI	CCNNGG	3 cut(s)	331, 351, 591
Bsc4I	CCNNNNNNNGG	1 cut(s)	664
Bse3DI	GCAATG	2 cut(s)	12, 17
Bse8I	GATNNNNATC	1 cut(s)	672
BseBI	CCWGG	1 cut(s)	1403
BseDI	CCNNGG	3 cut(s)	331, 351, 591
BseGI	GGATG	2 cut(s)	673, 799
BseJI	GATNNNNATC	1 cut(s)	672
BseLI	CCNNNNNNNGG	1 cut(s)	664
BseMI	GCAATG	2 cut(s)	12, 17
BseMII	CTCAG	3 cut(s)	161, 465, 746
BseRI	GAGGAG	3 cut(s)	611, 1121, 1367
BseXI	GCAGC	5 cut(s)	424, 667, 695, 848, 868
BseYI	CCCAGC	1 cut(s)	429
BsgI	GTGCAG	2 cut(s)	239, 528
Bsh1236I	CGCG	1 cut(s)	62
BshFI	GGCC	2 cut(s)	350, 776
BsiHKAI	GWGCWC	3 cut(s)	646, 988, 1038
BsiHKCI	CYCGRG	2 cut(s)	444, 884
BslFI	GGGAC	1 cut(s)	462
BslI	CCNNNNNNNGG	1 cut(s)	664
BsmAI	GTCTC	2 cut(s)	183, 756
BsmBI	CGTCTC	1 cut(s)	756
BsmFI	GGGAC	1 cut(s)	462
BsmI	GAATGC	1 cut(s)	561
BsnI	GGCC	2 cut(s)	350, 776
BsoBI	CYCGRG	2 cut(s)	444, 884
Bsp1286I	GDGCHC	3 cut(s)	646, 988, 1038
BspACI	CCGC	3 cut(s)	607, 912, 961
BspANI	GGCC	2 cut(s)	350, 776
BspCNI	CTCAG	3 cut(s)	160, 466, 747
BspFNI	CGCG	1 cut(s)	62
BspLI	GGNNCC	3 cut(s)	36, 329, 1227
BspMAI	CTGCAG	1 cut(s)	685
BspOI	GCTAGC	1 cut(s)	1310
BspPI	GGATC	5 cut(s)	233, 792, 903, 1220, 1233
BspQI	GCTCTTC	2 cut(s)	1026, 1296
BsrDI	GCAATG	2 cut(s)	12, 17
BssECI	CCNNGG	3 cut(s)	331, 351, 591
BssT1I	CCWWGG	3 cut(s)	331, 351, 591
Bst2UI	CCWGG	1 cut(s)	1403
Bst4CI	ACNGT	6 cut(s)	260, 273, 324, 1047, 1073, 1314
Bst6I	CTCTTC	2 cut(s)	1026, 1296
BstAPI	GCANNNNNTGC	1 cut(s)	572
BstBAI	YACGTR	1 cut(s)	641
BstC8I	GCNNGC	6 cut(s)	24, 64, 93, 222, 963, 1308
BstDEI	CTNAG	5 cut(s)	147, 474, 755, 871, 938
BstEII	GGTNACC	1 cut(s)	284
BstF5I	GGATG	2 cut(s)	673, 799
BstFNI	CGCG	1 cut(s)	62
BstH2I	RGCGCY	1 cut(s)	1337
BstHHI	GCGC	3 cut(s)	76, 1291, 1336
BstMAI	GTCTC	2 cut(s)	183, 756
BstMWI	GCNNNNNNNGC	9 cut(s)	23, 217, 336, 372, 572, 581, 677, 920, 1288
BstNI	CCWGG	1 cut(s)	1403
BstNSI	RCATGY	1 cut(s)	1216
BstPI	GGTNACC	1 cut(s)	284
BstSCI	CCNGG	1 cut(s)	1401
BstSFI	CTRYAG	1 cut(s)	681
BstUI	CGCG	1 cut(s)	62
BstV1I	GCAGC	5 cut(s)	424, 667, 695, 848, 868
BstV2I	GAAGAC	1 cut(s)	1017
BstX2I	RGATCY	3 cut(s)	673, 784, 1225
BstYI	RGATCY	3 cut(s)	673, 784, 1225
BsuRI	GGCC	2 cut(s)	350, 776
BtrI	CACGTC	1 cut(s)	217
BtsCI	GGATG	2 cut(s)	673, 799
BtsIMutI	CAGTG	1 cut(s)	320
Cac8I	GCNNGC	6 cut(s)	24, 64, 93, 222, 963, 1308
CaiI	CAGNNNCTG	1 cut(s)	1267
CfoI	GCGC	3 cut(s)	76, 1291, 1336
Cfr13I	GGNCC	1 cut(s)	775
CseI	GACGC	1 cut(s)	345
Csp6I	GTAC	2 cut(s)	58, 638
CviAII	CATG	9 cut(s)	77, 182, 290, 647, 853, 924, 957, 1213, 1364
CviQI	GTAC	2 cut(s)	58, 638
DdeI	CTNAG	5 cut(s)	147, 474, 755, 871, 938
DraI	TTTAAA	1 cut(s)	229
Eam1104I	CTCTTC	2 cut(s)	1026, 1296
EarI	CTCTTC	2 cut(s)	1026, 1296
EciI	GGCGGA	1 cut(s)	901
Ecl136II	GAGCTC	2 cut(s)	986, 1036
Eco130I	CCWWGG	3 cut(s)	331, 351, 591
Eco24I	GRGCYC	2 cut(s)	988, 1038
Eco47III	AGCGCT	1 cut(s)	1335
Eco53kI	GAGCTC	2 cut(s)	986, 1036
Eco57I	CTGAAG	2 cut(s)	764, 773
Eco88I	CYCGRG	2 cut(s)	444, 884
Eco91I	GGTNACC	1 cut(s)	284
EcoICRI	GAGCTC	2 cut(s)	986, 1036
EcoO109I	RGGNCCY	1 cut(s)	775
EcoO65I	GGTNACC	1 cut(s)	284
EcoRII	CCWGG	1 cut(s)	1401
EcoT14I	CCWWGG	3 cut(s)	331, 351, 591
EcoT22I	ATGCAT	1 cut(s)	183
EcoT38I	GRGCYC	2 cut(s)	988, 1038
ErhI	CCWWGG	3 cut(s)	331, 351, 591
Esp3I	CGTCTC	1 cut(s)	756
FaeI	CATG	9 cut(s)	80, 185, 293, 650, 856, 927, 960, 1216, 1367
FalI	AAGNNNNNCTT	2 cut(s)	921, 953
FaqI	GGGAC	1 cut(s)	462
FatI	CATG	9 cut(s)	76, 181, 289, 646, 852, 923, 956, 1212, 1363
FauI	CCCGC	1 cut(s)	954
FbaI	TGATCA	1 cut(s)	1186
FblI	GTMKAC	1 cut(s)	890
Fnu4HI	GCNGC	5 cut(s)	438, 681, 684, 837, 857
FokI	GGATG	2 cut(s)	680, 806
FriOI	GRGCYC	2 cut(s)	988, 1038
Fsp4HI	GCNGC	5 cut(s)	438, 681, 684, 837, 857
FspBI	CTAG	4 cut(s)	677, 821, 1128, 1307
GlaI	GCGC	3 cut(s)	75, 1290, 1335
GluI	GCNGC	5 cut(s)	438, 681, 684, 837, 857
GsaI	CCCAGC	1 cut(s)	433
GsuI	CTGGAG	2 cut(s)	1026, 1379
HaeII	RGCGCY	1 cut(s)	1337
HaeIII	GGCC	2 cut(s)	350, 776
HgaI	GACGC	1 cut(s)	345
HhaI	GCGC	3 cut(s)	76, 1291, 1336
Hin1II	CATG	9 cut(s)	80, 185, 293, 650, 856, 927, 960, 1216, 1367
Hin6I	GCGC	3 cut(s)	74, 1289, 1334
HinP1I	GCGC	3 cut(s)	74, 1289, 1334
HincII	GTYRAC	1 cut(s)	934
HindII	GTYRAC	1 cut(s)	934
HinfI	GANTC	6 cut(s)	530, 692, 887, 1169, 1264, 1270
HpaI	GTTAAC	1 cut(s)	934
HphI	GGTGA	2 cut(s)	389, 896
Hpy166II	GTNNAC	3 cut(s)	391, 891, 934
Hpy188I	TCNGA	5 cut(s)	486, 535, 697, 756, 1269
Hpy8I	GTNNAC	3 cut(s)	391, 891, 934
HpyAV	CCTTC	6 cut(s)	25, 658, 788, 1054, 1105, 1163
HpyCH4III	ACNGT	6 cut(s)	260, 273, 324, 1047, 1073, 1314
HpyCH4IV	ACGT	3 cut(s)	216, 235, 640
HpyF10VI	GCNNNNNNNGC	9 cut(s)	23, 217, 336, 372, 572, 581, 677, 920, 1288
HpyF3I	CTNAG	5 cut(s)	147, 474, 755, 871, 938
HpySE526I	ACGT	3 cut(s)	216, 235, 640
Hsp92II	CATG	9 cut(s)	80, 185, 293, 650, 856, 927, 960, 1216, 1367
HspAI	GCGC	3 cut(s)	74, 1289, 1334
Ksp22I	TGATCA	1 cut(s)	1186
KspAI	GTTAAC	1 cut(s)	934
LguI	GCTCTTC	2 cut(s)	1026, 1296
LmnI	GCTCC	4 cut(s)	34, 327, 1354, 1360
Lsp1109I	GCAGC	5 cut(s)	424, 667, 695, 848, 868
LweI	GCATC	8 cut(s)	75, 168, 553, 562, 658, 784, 823, 1007
MaeI	CTAG	4 cut(s)	677, 821, 1128, 1307
MaeII	ACGT	3 cut(s)	216, 235, 640
MaeIII	GTNAC	3 cut(s)	284, 703, 1314
MboII	GAAGA	8 cut(s)	618, 779, 962, 993, 1022, 1043, 1076, 1313
MflI	RGATCY	3 cut(s)	673, 784, 1225
MhlI	GDGCHC	3 cut(s)	646, 988, 1038
MlyI	GAGTC	3 cut(s)	686, 896, 1178
Mph1103I	ATGCAT	1 cut(s)	183
MseI	TTAA	7 cut(s)	228, 612, 843, 933, 980, 1238, 1411
MslI	CAYNNNNRTG	1 cut(s)	1211
MspA1I	CMGCKG	2 cut(s)	151, 526
MspR9I	CCNGG	1 cut(s)	1403
Mva1269I	GAATGC	1 cut(s)	561
MvaI	CCWGG	1 cut(s)	1403
MvnI	CGCG	1 cut(s)	62
MwoI	GCNNNNNNNGC	9 cut(s)	23, 217, 336, 372, 572, 581, 677, 920, 1288
NheI	GCTAGC	1 cut(s)	1306
NlaIII	CATG	9 cut(s)	80, 185, 293, 650, 856, 927, 960, 1216, 1367
NlaIV	GGNNCC	3 cut(s)	36, 329, 1227
NmeAIII	GCCGAG	2 cut(s)	187, 392
NmuCI	GTSAC	1 cut(s)	1314
NsiI	ATGCAT	1 cut(s)	183
NspI	RCATGY	1 cut(s)	1216
PaeR7I	CTCGAG	1 cut(s)	884
PciSI	GCTCTTC	2 cut(s)	1026, 1296
PctI	GAATGC	1 cut(s)	561
PfeI	GAWTC	3 cut(s)	530, 1264, 1270
PkrI	GCNGC	5 cut(s)	439, 682, 685, 838, 858
PleI	GAGTC	3 cut(s)	686, 895, 1177
PpsI	GAGTC	3 cut(s)	686, 895, 1177
Ppu21I	YACGTR	1 cut(s)	641
PsiI	TTATAA	1 cut(s)	810
Psp124BI	GAGCTC	2 cut(s)	988, 1038
Psp6I	CCWGG	1 cut(s)	1401
PspEI	GGTNACC	1 cut(s)	284
PspFI	CCCAGC	1 cut(s)	429
PspGI	CCWGG	1 cut(s)	1401
PspN4I	GGNNCC	3 cut(s)	36, 329, 1227
PspPI	GGNCC	1 cut(s)	775
PspXI	VCTCGAGB	1 cut(s)	884
PstI	CTGCAG	1 cut(s)	685
PstNI	CAGNNNCTG	1 cut(s)	1267
PsuI	RGATCY	3 cut(s)	673, 784, 1225
PvuII	CAGCTG	2 cut(s)	151, 526
RsaI	GTAC	2 cut(s)	59, 639
RsaNI	GTAC	2 cut(s)	58, 638
RseI	CAYNNNNRTG	1 cut(s)	1211
SacI	GAGCTC	2 cut(s)	988, 1038
SapI	GCTCTTC	2 cut(s)	1026, 1296
SaqAI	TTAA	7 cut(s)	228, 612, 843, 933, 980, 1238, 1411
SatI	GCNGC	5 cut(s)	438, 681, 684, 837, 857
Sau96I	GGNCC	1 cut(s)	775
SchI	GAGTC	3 cut(s)	686, 896, 1178
ScrFI	CCNGG	1 cut(s)	1403
SduI	GDGCHC	3 cut(s)	646, 988, 1038
SfaNI	GCATC	8 cut(s)	75, 168, 553, 562, 658, 784, 823, 1007
SfcI	CTRYAG	1 cut(s)	681
Sfr274I	CTCGAG	1 cut(s)	884
SlaI	CTCGAG	1 cut(s)	884
SmiMI	CAYNNNNRTG	1 cut(s)	1211
SmlI	CTYRAG	3 cut(s)	278, 713, 884
SmoI	CTYRAG	3 cut(s)	278, 713, 884
SsiI	CCGC	3 cut(s)	607, 912, 961
SspMI	CTAG	4 cut(s)	677, 821, 1128, 1307
SstI	GAGCTC	2 cut(s)	988, 1038
StyD4I	CCNGG	1 cut(s)	1401
StyI	CCWWGG	3 cut(s)	331, 351, 591
TaaI	ACNGT	6 cut(s)	260, 273, 324, 1047, 1073, 1314
TaiI	ACGT	3 cut(s)	219, 238, 643
TaqI	TCGA	3 cut(s)	8, 307, 885
TfiI	GAWTC	3 cut(s)	530, 1264, 1270
Tru1I	TTAA	7 cut(s)	228, 612, 843, 933, 980, 1238, 1411
Tru9I	TTAA	7 cut(s)	228, 612, 843, 933, 980, 1238, 1411
TscAI	CASTG	1 cut(s)	327
TseFI	GTSAC	1 cut(s)	1314
TseI	GCWGC	5 cut(s)	437, 680, 683, 836, 856
Tsp45I	GTSAC	1 cut(s)	1314
TspDTI	ATGAA	4 cut(s)	633, 1065, 1145, 1380
TspRI	CASTG	1 cut(s)	327
XapI	RAATTY	2 cut(s)	620, 1122
XbaI	TCTAGA	1 cut(s)	1127
XceI	RCATGY	1 cut(s)	1216
XhoI	CTCGAG	1 cut(s)	884
XmiI	GTMKAC	1 cut(s)	890
XspI	CTAG	4 cut(s)	677, 821, 1128, 1307
Zsp2I	ATGCAT	1 cut(s)	183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.